BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,970,026	C→A	21.0%	A130D (GCC→GAC)	wzxE →	O‑antigen translocase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,970,026	0	C	A	21.0%	1058.4 / 213.0	389	A130D (GCC→GAC)	wzxE	O‑antigen translocase
Reads supporting (aligned to +/- strand): ref base C (156/151); new base A (30/52); total (186/203)
Fisher's exact test for biased strand distribution p-value = 2.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 397 total aligned reads displayed.

CGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGG  >  CP009273/3969877‑3970175

cGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCACATGGGGATCGc                                                                                                                                                       >  2:2761179/1‑150 (MQ=255)

   cACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTg                                                                                                                                                    <  2:3993780/150‑1 (MQ=255)

    aCTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgg                                                                                                                                                   <  1:2808809/150‑1 (MQ=255)

    aCTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgg                                                                                                                                                   <  2:4441582/150‑1 (MQ=255)

     cTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTggg                                                                                                                                                  >  2:3019523/1‑150 (MQ=255)

     cTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTggg                                                                                                                                                  <  1:4227485/150‑1 (MQ=255)

        catcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCa                                                                                                                                               <  1:444259/150‑1 (MQ=255)

          tcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAAc                                                                                                                                             <  1:2772252/150‑1 (MQ=255)

          tcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAAc                                                                                                                                             <  2:4019108/150‑1 (MQ=255)

          tcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAAc                                                                                                                                             >  2:1047236/1‑150 (MQ=255)

             gCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTg                                                                                                                                          >  1:3070319/1‑150 (MQ=255)

               gATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGtt                                                                                                                                        <  1:347594/150‑1 (MQ=255)

               gATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGtt                                                                                                                                        <  2:155513/150‑1 (MQ=255)

                 tGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCACCCTGTTAc                                                                                                                                      >  2:2602541/1‑150 (MQ=255)

                   gTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTg                                                                                                                                    <  1:493589/150‑1 (MQ=255)

                        tGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCt                                                                                                                               >  1:1705678/1‑150 (MQ=255)

                         ggTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCtg                                                                                                                              >  2:4361162/1‑150 (MQ=255)

                            ttCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCtgatg                                                                                                                           >  1:576798/1‑150 (MQ=255)

                             tctcTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCtgatga                                                                                                                          >  1:4605616/1‑150 (MQ=255)

                              ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                                                                                                         >  2:1218469/1‑150 (MQ=255)

                               tctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaaa                                                                                                                        <  2:4497465/150‑1 (MQ=255)

                                ctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAg                                                                                                                       >  1:3007455/1‑150 (MQ=255)

                                 tACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAgg                                                                                                                      <  2:1518972/150‑1 (MQ=255)

                                 tACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAgg                                                                                                                      <  2:3885295/150‑1 (MQ=255)

                                   cGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCt                                                                                                                    <  1:1932614/150‑1 (MQ=255)

                                     cTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCttt                                                                                                                  <  2:4620458/150‑1 (MQ=255)

                                     cTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCttt                                                                                                                  >  1:4363779/1‑150 (MQ=255)

                                       gATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcg                                                                                                                >  2:3823132/1‑150 (MQ=255)

                                       gATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcg                                                                                                                <  2:1119276/150‑1 (MQ=255)

                                             gcTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGc                                                                                                          <  1:3106137/150‑1 (MQ=255)

                                              cTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGcc                                                                                                         <  2:2709337/150‑1 (MQ=255)

                                               tGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCg                                                                                                        <  1:2356653/150‑1 (MQ=255)

                                                   ttttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAgg                                                                                                    <  2:2942355/150‑1 (MQ=255)

                                                    tttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGt                                                                                                   <  2:1975863/150‑1 (MQ=255)

                                                     ttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTa                                                                                                  <  2:2224766/150‑1 (MQ=255)

                                                      tgtgCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTaa                                                                                                 >  2:745376/1‑150 (MQ=255)

                                                      tgtgCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTaa                                                                                                 >  2:2164865/1‑150 (MQ=255)

                                                         gCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGc                                                                                              <  1:3849656/150‑1 (MQ=255)

                                                         gCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGc                                                                                              <  1:478853/150‑1 (MQ=255)

                                                              cAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTAt                                                                                         <  1:82044/150‑1 (MQ=255)

                                                               aGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATc                                                                                        <  1:4086350/150‑1 (MQ=255)

                                                               aGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATc                                                                                        <  1:2572785/150‑1 (MQ=255)

                                                                 cTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGc                                                                                      >  1:3168366/1‑150 (MQ=255)

                                                                     gcCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGAt                                                                                  <  2:2589031/150‑1 (MQ=255)

                                                                      ccAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGAtt                                                                                 <  1:337267/150‑1 (MQ=255)

                                                                      ccAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGAtt                                                                                 >  1:4314791/1‑150 (MQ=255)

                                                                        aaTCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGt                                                                               >  1:3522794/1‑150 (MQ=255)

                                                                         aTCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTc                                                                              >  2:603725/1‑150 (MQ=255)

                                                                         aTCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTc                                                                              <  2:3187926/150‑1 (MQ=255)

                                                                         aTCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTc                                                                              <  2:3352149/150‑1 (MQ=255)

                                                                         aTCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGGTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTc                                                                              >  1:1355092/1‑150 (MQ=255)

                                                                            aGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGc                                                                           >  1:3306009/1‑150 (MQ=255)

                                                                             gCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCa                                                                          <  2:4152739/150‑1 (MQ=255)

                                                                              ccAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAg                                                                         <  1:1664443/150‑1 (MQ=255)

                                                                              ccAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAg                                                                         <  1:2286119/150‑1 (MQ=255)

                                                                               cAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGc                                                                        >  1:2044404/1‑150 (MQ=255)

                                                                               cAGGGATTGTTTGGTAATACCGACTATCAGGGGCGGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGc                                                                        >  2:1096139/1‑150 (MQ=255)

                                                                                aGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCt                                                                       >  2:1443471/1‑150 (MQ=255)

                                                                                   gATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGa                                                                    >  1:2155266/1‑150 (MQ=255)

                                                                                     ttGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGAtt                                                                  <  2:1590085/150‑1 (MQ=255)

                                                                                      tGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTg                                                                 <  1:4155897/150‑1 (MQ=255)

                                                                                       gTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTgg                                                                >  1:1855606/1‑150 (MQ=255)

                                                                                        tttGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGc                                                               <  2:3310899/150‑1 (MQ=255)

                                                                                            gTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTtc                                                           <  1:3844298/150‑1 (MQ=255)

                                                                                             tAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTtct                                                          >  2:2344429/1‑150 (MQ=255)

                                                                                              aaTACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTtctc                                                         <  2:3841668/150‑1 (MQ=255)

                                                                                                tACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTcgc                                                       >  2:869221/1‑150 (MQ=255)

                                                                                                 aCCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTcgcg                                                      >  1:725449/1‑150 (MQ=255)

                                                                                                   cGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGta                                                    <  2:4301812/150‑1 (MQ=255)

                                                                                                       tATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGtactac                                                >  1:2213716/1‑150 (MQ=255)

                                                                                                           aGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGCTCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCa                                            >  2:3349160/1‑150 (MQ=255)

                                                                                                           aGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCa                                            >  2:345543/1‑150 (MQ=255)

                                                                                                           aGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCa                                            <  2:1624426/150‑1 (MQ=255)

                                                                                                              ggCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGtt                                         <  2:2840289/150‑1 (MQ=255)

                                                                                                                cTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTAc                                       <  2:280919/150‑1 (MQ=255)

                                                                                                                cTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTAc                                       <  2:369365/150‑1 (MQ=255)

                                                                                                                 tGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTAcc                                      >  2:2544017/1‑150 (MQ=255)

                                                                                                                 tGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTAcc                                      >  1:2484186/1‑150 (MQ=255)

                                                                                                                 tGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTAcc                                      >  1:91689/1‑150 (MQ=255)

                                                                                                                 tGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTAcc                                      >  2:1691851/1‑150 (MQ=255)

                                                                                                                  ggTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCg                                     >  2:2523074/1‑150 (MQ=255)

                                                                                                                   gTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGt                                    >  1:3712266/1‑150 (MQ=255)

                                                                                                                      cGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTg                                 <  1:150023/150‑1 (MQ=255)

                                                                                                                       gTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTgg                                >  1:1889437/1‑150 (MQ=255)

                                                                                                                       gTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTgg                                >  2:3114187/1‑150 (MQ=255)

                                                                                                                        tttAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTggg                               >  2:2018815/1‑150 (MQ=255)

                                                                                                                           aGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCgg                            >  1:4514033/1‑150 (MQ=255)

                                                                                                                              ggCGCTGGTGCAACTGGGGATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTa                         <  1:105919/150‑1 (MQ=255)

                                                                                                                               gcgcTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTAt                        <  2:4737157/150‑1 (MQ=255)

                                                                                                                                      tGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAgggg                 >  1:1429340/1‑150 (MQ=255)

                                                                                                                                         aaaTGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGt              <  2:3799524/150‑1 (MQ=255)

                                                                                                                                         aaaTGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGt              >  2:562555/1‑150 (MQ=255)

                                                                                                                                          aaTGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGtt             <  1:4662290/150‑1 (MQ=255)

                                                                                                                                             ggggATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCt          >  1:3506603/1‑150 (MQ=255)

                                                                                                                                             ggggATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCt          >  1:1105738/1‑150 (MQ=255)

                                                                                                                                                gATCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTggg       >  2:3964879/1‑150 (MQ=255)

                                                                                                                                                 aTCGACTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGt      >  1:3204058/1‑150 (MQ=255)

                                                                                                                                                     ccTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTgg  <  2:4234316/150‑1 (MQ=255)

                                                                                                                                                     ccTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTgg  >  2:133051/1‑150 (MQ=255)

                                                                                                                                                     ccTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTgg  <  2:1829410/150‑1 (MQ=255)

CGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGG  >  CP009273/3969877‑3970175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`