Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,062,424 G→T 93.0% I71I (ATC→ATA)  yihQ ← alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,062,4240GT93.0% 792.3 / 48.0 257I71I (ATC→ATA) yihQalpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base G (3/15);  new base T (87/152);  total (90/167)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 257 total aligned reads displayed.

CGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTA  >  CP009273/4062278‑4062572
                                                                                                                                                  |                                                                                                                                                    
cgcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATg                                                                                                                                                   <  1:4660141/150‑1 (MQ=255)
 gcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATgg                                                                                                                                                  <  1:3980357/150‑1 (MQ=255)
 gcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATgg                                                                                                                                                  <  1:2508944/150‑1 (MQ=255)
  cAGCCAGATACGTTTGTGGTTATGGTTTTCGTTTGGTAGGTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGc                                                                                                                                                 <  2:8199/150‑1 (MQ=255)
  cAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGc                                                                                                                                                 <  2:525507/150‑1 (MQ=255)
  cAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGc                                                                                                                                                 <  2:3822849/150‑1 (MQ=255)
    gCCAGATAGGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGt                                                                                                                                               <  1:3632743/150‑1 (MQ=255)
     ccAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTc                                                                                                                                              <  1:3722876/150‑1 (MQ=255)
      cAGATACGGTTGTGGTTCAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCg                                                                                                                                             >  2:2333700/1‑150 (MQ=255)
      cAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCg                                                                                                                                             >  2:429758/1‑150 (MQ=255)
       aGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCgg                                                                                                                                            <  2:1330598/150‑1 (MQ=255)
       aGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCgg                                                                                                                                            <  2:1053795/150‑1 (MQ=255)
        gATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGt                                                                                                                                           <  2:1677822/150‑1 (MQ=255)
        gATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGt                                                                                                                                           <  2:4083016/150‑1 (MQ=255)
          tACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTaa                                                                                                                                         <  1:1524642/150‑1 (MQ=255)
            gggTTGTGGTTAAGGGTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgc                                                                                                                                       <  1:3350003/149‑1 (MQ=255)
             ggTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAgcg                                                                                                                                      <  1:1457562/150‑1 (MQ=255)
                tgtgGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCaa                                                                                                                                   <  2:1857944/150‑1 (MQ=255)
                  tgtttAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtt                                                                                                                                 <  1:2659557/147‑1 (MQ=255)
                  tgGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATACACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtt                                                                                                                                 <  1:4484453/150‑1 (MQ=255)
                    gTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtttt                                                                                                                               >  1:869132/1‑150 (MQ=255)
                    gTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtttt                                                                                                                               >  2:1018611/1‑150 (MQ=255)
                    gTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAAtttt                                                                                                                               <  2:346965/150‑1 (MQ=255)
                     ttAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTtc                                                                                                                              >  1:1746334/1‑150 (MQ=255)
                      tAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTtct                                                                                                                             <  1:1398607/150‑1 (MQ=255)
                      tAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTtct                                                                                                                             >  2:2677960/1‑150 (MQ=255)
                      tAAGGTTGTCGTATTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTtct                                                                                                                             >  1:1676097/1‑150 (MQ=255)
                         ggTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCt                                                                                                                          >  1:4777236/1‑150 (MQ=255)
                           ttGTCGTTTTGTAGTTCCAGCAATACACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTgg                                                                                                                        <  1:2125414/150‑2 (MQ=255)
                           ttGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGt                                                                                                                        >  1:2721268/1‑150 (MQ=255)
                             gTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAg                                                                                                                      <  1:2988372/150‑1 (MQ=255)
                              tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGt                                                                                                                     <  2:1474253/150‑1 (MQ=255)
                              tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGt                                                                                                                     <  2:4593812/150‑1 (MQ=255)
                              tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGt                                                                                                                     <  2:3743434/150‑1 (MQ=255)
                               cGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGtt                                                                                                                    <  2:1778498/150‑1 (MQ=255)
                                 ttttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTa                                                                                                                  >  1:1844023/1‑150 (MQ=255)
                                  tttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTAt                                                                                                                 <  1:1300440/150‑1 (MQ=255)
                                   ttGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATc                                                                                                                >  1:188024/1‑150 (MQ=255)
                                      tAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCttt                                                                                                             <  2:123852/150‑1 (MQ=255)
                                          tCCAGAAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATg                                                                                                         <  1:2431278/150‑1 (MQ=255)
                                           ccAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGc                                                                                                        >  1:802416/1‑150 (MQ=255)
                                            cAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCt                                                                                                       >  1:660070/1‑150 (MQ=255)
                                             aGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTg                                                                                                      <  1:1306497/150‑1 (MQ=255)
                                               cAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaa                                                                                                    <  2:935157/150‑1 (MQ=255)
                                                  tAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAtt                                                                                                 <  2:3140936/150‑1 (MQ=255)
                                                      cGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTACCCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcg                                                                                             <  2:3434741/150‑1 (MQ=255)
                                                      cGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcg                                                                                             >  2:4524796/1‑150 (MQ=255)
                                                      cGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcg                                                                                             <  1:2817605/150‑1 (MQ=255)
                                                       gCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcgc                                                                                            <  1:2682389/150‑1 (MQ=255)
                                                              aTCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACat                                                                                     <  1:457296/150‑1 (MQ=255)
                                                                cgtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACatat                                                                                   >  2:1757130/1‑150 (MQ=255)
                                                                 gtcgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATc                                                                                  >  1:474810/1‑150 (MQ=255)
                                                                      cAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGNAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTNAAATTACCGCGGAACATATCGatat                                                                             >  1:501424/1‑150 (MQ=255)
                                                                      cAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGatat                                                                             >  2:4440991/1‑150 (MQ=255)
                                                                        gagaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATCTCGATATcc                                                                           >  2:4425388/1‑150 (MQ=255)
                                                                         agaTATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCg                                                                          <  2:3345713/150‑1 (MQ=255)
                                                                           atatTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCt                                                                        <  1:2611582/150‑1 (MQ=255)
                                                                             atTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCtat                                                                      >  1:3851334/1‑150 (MQ=255)
                                                                                cAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAcc                                                                   <  1:239227/150‑1 (MQ=255)
                                                                                 aGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCt                                                                  <  2:4220077/150‑1 (MQ=255)
                                                                                        cgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCaa                                                           >  1:1070192/1‑150 (MQ=255)
                                                                                        cgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCaa                                                           >  1:3549128/1‑150 (MQ=255)
                                                                                        cgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCaa                                                           <  1:1290469/150‑1 (MQ=255)
                                                                                         gcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAt                                                          <  2:1039429/150‑1 (MQ=255)
                                                                                          cTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATc                                                         >  2:4358458/1‑150 (MQ=255)
                                                                                           tAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATcc                                                        <  2:3912647/150‑1 (MQ=255)
                                                                                           tAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGAGTCGGTAAGCGCAATTTTCTCCTGGAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCAATACCTGAGCCACTcc                                                        >  2:3091670/1‑150 (MQ=255)
                                                                                            aaTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCa                                                       <  2:1069353/150‑1 (MQ=255)
                                                                                             aTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCAt                                                      <  2:4460149/150‑1 (MQ=255)
                                                                                             aTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCAt                                                      <  1:1756196/150‑1 (MQ=255)
                                                                                              tGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATa                                                     <  2:3243038/150‑1 (MQ=255)
                                                                                              tGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATa                                                     <  2:188024/150‑1 (MQ=255)
                                                                                              tGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATa                                                     <  1:1132856/150‑1 (MQ=255)
                                                                                                tCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATaaa                                                   >  2:868666/1‑150 (MQ=255)
                                                                                                  aGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACa                                                 <  1:1651880/150‑1 (MQ=255)
                                                                                                   gAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACaa                                                >  2:2465657/1‑150 (MQ=255)
                                                                                                    aaCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAg                                               <  2:4479005/150‑1 (MQ=255)
                                                                                                     aCCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAgg                                              <  2:474810/150‑1 (MQ=255)
                                                                                                      ccACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGa                                             >  2:3952007/1‑150 (MQ=255)
                                                                                                       cACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGAt                                            <  1:683894/150‑1 (MQ=255)
                                                                                                       cACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGAt                                            <  2:720064/150‑1 (MQ=255)
                                                                                                        aCGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGAtt                                           >  1:1226656/1‑150 (MQ=255)
                                                                                                        aCGGCGGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGGAGTTTATCGTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGAtt                                           >  1:2903084/1‑150 (MQ=255)
                                                                                                         cGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGATCCAATCCATAAACAAGGATTa                                          >  1:2021025/1‑150 (MQ=255)
                                                                                                         cGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTa                                          <  2:672166/150‑1 (MQ=255)
                                                                                                          ggCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTAt                                         <  2:58432/150‑1 (MQ=255)
                                                                                                             tGAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATACATAAACAAGGATTATCtt                                      >  2:2858085/1‑150 (MQ=255)
                                                                                                              gAAATGAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCttt                                     <  1:4173601/150‑1 (MQ=255)
                                                                                                                   gAATTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTAt                                <  2:1648122/150‑1 (MQ=255)
                                                                                                                    aaTTAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATg                               <  2:3993268/150‑1 (MQ=255)
                                                                                                                       tAACCAACCATCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGt                            <  2:2036571/150‑1 (MQ=255)
                                                                                                                            aaccaTCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTaaaa                       <  1:3180082/150‑1 (MQ=255)
                                                                                                                              ccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATa                     <  2:369189/150‑1 (MQ=255)
                                                                                                                              ccaTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATa                     >  1:4218296/1‑150 (MQ=255)
                                                                                                                               caTCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATaa                    <  1:4440991/150‑1 (MQ=255)
                                                                                                                                 tCCGGTGACTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGa                  <  1:948930/150‑1 (MQ=255)
                                                                                                                                         cTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGttgttg          <  1:1018611/150‑1 (MQ=255)
                                                                                                                                         cTGGCTGACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGttgttg          >  1:1038508/1‑150 (MQ=255)
                                                                                                                                               gACTATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATg    <  2:354783/150‑1 (MQ=255)
                                                                                                                                                 cGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTTAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTa  >  1:1812083/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGGTTAAAATAAGACGTTGTTGAAAATGTA  >  CP009273/4062278‑4062572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑