Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,066,133 T→G 100% S267R (AGC→CGC)  yihU ← gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,066,1330TG96.5% 1435.6 / 19.8 371S267R (AGC→CGC) yihUgamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (5/8);  new base G (207/151);  total (212/159)
Fisher's exact test for biased strand distribution p-value = 2.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 375 total aligned reads displayed.

TAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAG  >  CP009273/4065984‑4066279
                                                                                                                                                     |                                                                                                                                                  
tAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCt                                                                                                                                                    <  1:3474352/150‑1 (MQ=255)
   tGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCgata                                                                                                                                                 <  1:434747/150‑1 (MQ=255)
   tGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTAAATTTGCAGTCAACCCCGCACTGACACCGAACAGTTCCAGAAAGGCGGACCAGACCAGGCGACCGCGACCCCCTGCGCGCGCCAGGCgata                                                                                                                                                 >  2:644616/1‑150 (MQ=255)
     tCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTAAATTTTTAATTTTGCAGTCAAACCCGCACAGAAACCGACCAGGTACAGAATGGCGGACCACTCCAGGCGCCCCCGACCCGCGGCGCGCGCCAGGCGCTaaa                                                                                                                                               >  1:1749938/1‑150 (MQ=255)
         tGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTAAAATTTTAATTTTTCAGTCATCCCGGCACTGAAAAGGACCAGTTACAGAAAGTCGGACCCCTCCAGGCGACCCCGACCCCCCGCGCGCGCCAGGCGAAAAAccac                                                                                                                                           >  2:3422610/1‑148 (MQ=255)
           aTGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTccc                                                                                                                                         <  2:520804/150‑1 (MQ=255)
            tgttttACTTATTCATTCATTTATTCCTTTATCCAGTCGTTAAATTTTTACTTTGGCAGTCTTCCCGGCAATGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCTATGCGCTCGCCTGGCGATAAACCTccct                                                                                                                                        <  2:2325159/145‑2 (MQ=255)
            tGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCg                                                                                                                                        <  2:461703/150‑1 (MQ=255)
             ggTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTAATTTTGCAGTAAAACCGGCACAGACACGGACCTGTTACAGAATTGCGGACCAGTCCTGGCGACCGCGACCCCCAGCGCGCGCCAGGCGATAAACCAcccct                                                                                                                                       >  2:583380/1‑148 (MQ=255)
                gtACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAg                                                                                                                                    <  2:4748944/150‑1 (MQ=255)
                       ttcattcaTTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTACAGAATGGCGGTCCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCAGGCGATAAACCACCCGTGGGGCGGccc                                                                                                                             >  2:4542563/1‑150 (MQ=255)
                         cattcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTTGCAGTAATACCGGCACTGACACGGACCTGTTCCAGAATTGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCCTGAGGCGGccccc                                                                                                                           >  2:3876360/1‑150 (MQ=255)
                          attcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCa                                                                                                                          <  2:1397090/150‑1 (MQ=255)
                          attcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCa                                                                                                                          <  1:4122220/150‑1 (MQ=255)
                          attcattTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCa                                                                                                                          <  2:3548549/150‑1 (MQ=255)
                            tcattTATTCCTTTATCCAGTCGTTACATTTTTACATTGGCAGTAATACCCGCACTGACACCGACCAGTTCCACAAAGGCGGACCAGTCCAGGCGACCGCGAACCCCAGCGCGCGCCAGGCGATAAAACACCCGTGAGGCGGCCCCCacc                                                                                                                        >  2:3789010/1‑148 (MQ=255)
                             cattTATTCCTTTATCCAGTCGTTACATTTTTAATTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGCCCCCACCg                                                                                                                       >  2:4776862/1‑150 (MQ=255)
                              attTATTCCTTTATCCAGTCGTTACATTTTTAGTTTTGCCGTCAAACCCGCACAGACACGGACCAGTTCAAGAATTGCGGACCACTCCTGGCGACCGCGACCCCCGGCGCGCGCCAGGGGCTAAACCTCCCCTGAGGCGGCCCCCCGCgg                                                                                                                      >  2:4439054/1‑150 (MQ=255)
                               tttATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGc                                                                                                                     <  1:1018590/150‑1 (MQ=255)
                               tttATTCCTTTATCCAGTCGTTACATTTTTAATTTTGCAGTCAAACCGGCACTGACACGGACCTGTTCCAGAATTGCGGACCAGTACTTGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAAACAACCGTGAGGCGGCCCCCAGCGGc                                                                                                                     >  2:2733816/1‑150 (MQ=255)
                                  aTTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCAtt                                                                                                                  <  2:284486/150‑1 (MQ=255)
                                         tATCCAGTCATTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACAt                                                                                                           <  2:4593637/150‑1 (MQ=255)
                                           tCCAGTCGTGACATTTTTACTTTGGCAGTCATCCCGGCACTGACACCGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCACCCGTGAGGCGGCCCCCACCGGCATTGGCACATGc                                                                                                         >  2:3844094/1‑150 (MQ=255)
                                            ccAGTCGTTACATTTTTACTTTGGCAGTCATACCCGCACAGACACCGACCAGTTCCAGAATGGCGGACCACTCCAGGCGACCGCGACCCGCGGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCAATGGCACAAGCa                                                                                                        >  2:2205589/1‑150 (MQ=255)
                                             cAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAg                                                                                                       >  2:4079502/1‑150 (MQ=255)
                                             cAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAg                                                                                                       <  1:3989084/150‑1 (MQ=255)
                                             cAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCGGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAg                                                                                                       >  2:1340251/1‑150 (MQ=255)
                                                 cGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGTCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTgg                                                                                                   <  2:3082299/150‑1 (MQ=255)
                                                 cGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTgg                                                                                                   >  2:120930/1‑150 (MQ=255)
                                                     aCATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTgg                                                                                               <  1:2191533/150‑1 (MQ=255)
                                                      cATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGc                                                                                              >  1:97015/1‑150 (MQ=255)
                                                       aTTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCg                                                                                             >  2:3353024/1‑150 (MQ=255)
                                                         ttttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGACTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGAc                                                                                           <  1:1025080/150‑1 (MQ=255)
                                                         ttttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGAc                                                                                           >  2:3720998/1‑150 (MQ=255)
                                                         ttatACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGAAAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGAc                                                                                           >  2:1751033/4‑150 (MQ=255)
                                                          tttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACa                                                                                          <  1:4045459/150‑1 (MQ=255)
                                                          tttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACa                                                                                          >  2:2273573/1‑150 (MQ=255)
                                                          tttACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACa                                                                                          >  2:1274307/1‑150 (MQ=255)
                                                            tACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATc                                                                                        >  2:3649391/1‑150 (MQ=255)
                                                            gaCTTTGGCAGTCATCCCGGCACAGACACGGACCTGTTCCAGAATTGCGGACCAGTCCTGTCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATTCATCTTGTTGGCGACATc                                                                                        <  2:2113898/149‑1 (MQ=255)
                                                             aCTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCa                                                                                       >  1:2609598/1‑150 (MQ=255)
                                                              cTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCaa                                                                                      <  1:4289341/150‑1 (MQ=255)
                                                                ttGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAgg                                                                                    <  2:3326389/150‑1 (MQ=255)
                                                                     aGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGAt                                                                               >  2:1132151/1‑150 (MQ=255)
                                                                      gTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATg                                                                              <  2:4652046/150‑1 (MQ=255)
                                                                      gTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATg                                                                              >  2:2402183/1‑150 (MQ=255)
                                                                          tCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACCTCAAGGGCGATGCCaa                                                                          >  2:2914879/1‑150 (MQ=255)
                                                                          tCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCaa                                                                          >  2:735125/1‑150 (MQ=255)
                                                                             cGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGAt                                                                       <  1:2070498/150‑1 (MQ=255)
                                                                              ggCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATc                                                                      >  1:2318245/1‑150 (MQ=255)
                                                                              ggCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATc                                                                      >  2:3162377/1‑150 (MQ=255)
                                                                              ggCACTGACACGGACCGGTTCCAGACTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATc                                                                      <  1:911517/150‑1 (MQ=255)
                                                                                cACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCt                                                                    <  1:2088091/150‑1 (MQ=255)
                                                                                cACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCt                                                                    >  2:4774662/1‑150 (MQ=255)
                                                                                 aCTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATccgt                                                                   >  2:1644141/1‑148 (MQ=255)
                                                                                 aCTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCtt                                                                   >  1:1688511/1‑150 (MQ=255)
                                                                                   tGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                                 <  1:2705839/150‑1 (MQ=255)
                                                                                   tGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTAt                                                                 >  2:2606745/1‑150 (MQ=255)
                                                                                    gACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATg                                                                <  2:868758/150‑1 (MQ=255)
                                                                                       acGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGc                                                             >  2:1547678/1‑150 (MQ=255)
                                                                                       acGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGc                                                             <  1:2932057/150‑1 (MQ=255)
                                                                                        cGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCa                                                            >  1:4565597/1‑150 (MQ=255)
                                                                                         ggACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCaa                                                           >  1:1905109/1‑150 (MQ=255)
                                                                                          gACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAg                                                          <  1:3649214/150‑1 (MQ=255)
                                                                                             cTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatc                                                       >  1:3944540/1‑150 (MQ=255)
                                                                                             cTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatc                                                       >  1:3289708/1‑150 (MQ=255)
                                                                                             cTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatc                                                       <  1:1630203/150‑1 (MQ=255)
                                                                                             cTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatc                                                       >  1:1534030/1‑150 (MQ=255)
                                                                                               gTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcga                                                     <  1:2542476/150‑1 (MQ=255)
                                                                                                 tCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAgatcgatc                                                   >  1:3422288/1‑150 (MQ=255)
                                                                                                    aGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATg                                                <  1:2746612/150‑1 (MQ=255)
                                                                                                      aaTGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGaa                                              >  2:2759402/1‑150 (MQ=255)
                                                                                                        tGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAgg                                            <  2:97015/150‑1 (MQ=255)
                                                                                                            ggACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCggg                                        >  2:1518624/1‑150 (MQ=255)
                                                                                                            ggACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCggg                                        >  2:2049522/1‑150 (MQ=255)
                                                                                                            ggACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCggg                                        <  2:3614697/150‑1 (MQ=255)
                                                                                                            ggACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCggg                                        >  2:2542119/1‑150 (MQ=255)
                                                                                                              aCCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTGATGGGCAAGATCGATCATGAAGGCGGgag                                      >  2:4501594/1‑150 (MQ=255)
                                                                                                                cAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTCTGGGCACGATCGATCATGAAGGCGGGAGaa                                    >  1:782720/1‑150 (MQ=255)
                                                                                                                cAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGaa                                    >  2:1873073/1‑150 (MQ=255)
                                                                                                                cAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGaa                                    <  1:4494131/150‑1 (MQ=255)
                                                                                                                  gTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAg                                  >  2:1919946/1‑150 (MQ=255)
                                                                                                                     cTGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtc                               <  1:3353024/150‑1 (MQ=255)
                                                                                                                      tGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtct                              <  1:2139519/150‑1 (MQ=255)
                                                                                                                      tGGCGACCGCGACCCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGAtct                              >  2:4372764/1‑150 (MQ=255)
                                                                                                                        gcgaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCTTTGGCTCTTGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTcc                            <  1:2407541/150‑1 (MQ=255)
                                                                                                                          gaccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTGAGGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGc                          >  2:3401239/1‑150 (MQ=255)
                                                                                                                           accgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCt                         <  1:3508884/150‑1 (MQ=255)
                                                                                                                            ccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCCAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTg                        >  1:1228750/1‑150 (MQ=255)
                                                                                                                            ccgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTg                        >  2:2136348/1‑150 (MQ=255)
                                                                                                                             cgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCACGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGa                       >  2:1831212/1‑150 (MQ=255)
                                                                                                                             cgcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGa                       <  2:2452807/150‑1 (MQ=255)
                                                                                                                              gcgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAg                      <  1:4650290/150‑1 (MQ=255)
                                                                                                                               cgaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACAGGCAGCTGGTTGGCGACCTCAAGGGCGATGCCAAGATCCTTAGGGGCCCGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAgg                     >  2:1084171/1‑150 (MQ=255)
                                                                                                                                gaccCGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGa                    >  2:2298978/1‑150 (MQ=255)
                                                                                                                                    cGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACttt                <  1:2669066/150‑1 (MQ=255)
                                                                                                                                    cGCTGCGCGCGCCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACttt                <  1:317690/150‑1 (MQ=255)
                                                                                                                                          gcgcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTgg          <  1:515490/150‑1 (MQ=255)
                                                                                                                                           cgcgcCTGGCGATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGc         >  1:2101265/1‑150 (MQ=255)
                                                                                                                                              gcCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAg      >  1:4454569/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTGAGGCGGCCCCCAGCGGCATTGGCACATGCAGCTGGTTGGCGACATCAAGGGCGATGCCAAGATCCTTATGGGCAAGATCGATCATGAAGGCGGGAGAAAGATCTCCGCTGAGGACTTTGTTTGGCCAGGAAG  >  CP009273/4065984‑4066279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑