Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,220,948 G→T 29.4% D223Y (GAT→TAT)  rluF → 23S rRNA pseudouridine(2604) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,220,9480GT29.4% 410.0 / 149.7 240D223Y (GAT→TAT) rluF23S rRNA pseudouridine(2604) synthase
Reads supporting (aligned to +/- strand):  ref base G (95/71);  new base T (24/50);  total (119/121)
Fisher's exact test for biased strand distribution p-value = 4.56e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

GCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTC  >  CP009273/4220800‑4221095
                                                                                                                                                    |                                                                                                                                                   
gcCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGa                                                                                                                                                    >  1:2246945/1‑150 (MQ=255)
 ccGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGACTGGCGCGAt                                                                                                                                                   >  2:1357909/1‑150 (MQ=255)
 ccGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTAt                                                                                                                                                   <  2:2685352/150‑1 (MQ=255)
   gTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGAttt                                                                                                                                                 <  1:3003407/150‑1 (MQ=255)
   gTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGAttt                                                                                                                                                 >  2:3096909/1‑150 (MQ=255)
    tttGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTa                                                                                                                                                <  2:1574970/150‑1 (MQ=255)
     ttGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTaa                                                                                                                                               >  2:3109110/1‑150 (MQ=255)
       gTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAAcc                                                                                                                                             >  1:916477/1‑150 (MQ=255)
         cgtCCGCATTACCCTGGTGCAGGGGCTGACCCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCCTGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACcga                                                                                                                                           <  2:3639181/148‑1 (MQ=255)
            ccGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACcgacga                                                                                                                                        >  2:2068205/1‑150 (MQ=255)
            ccGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACcgacga                                                                                                                                        <  2:2110424/150‑1 (MQ=255)
            ccGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACcgacga                                                                                                                                        >  2:1121127/1‑150 (MQ=255)
            ccGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACcgacga                                                                                                                                        >  1:237837/1‑150 (MQ=255)
               cATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGa                                                                                                                                     >  1:2820159/1‑150 (MQ=255)
                aTTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAg                                                                                                                                    >  2:1069384/1‑150 (MQ=255)
                aTTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAg                                                                                                                                    >  1:2270015/1‑150 (MQ=255)
                  tACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGtt                                                                                                                                  >  2:1407261/1‑150 (MQ=255)
                   ccccGGGTGCAGGGGCTGAACCGTCAGAGCCGGCGATTGTGCGAGCATTTCGGCTATGACGTGAAAAAGCTGGAACGCACGCGCATCATGACCGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTa                                                                                                                                 <  1:4042386/149‑1 (MQ=255)
                   aCCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTa                                                                                                                                 >  1:869184/1‑150 (MQ=255)
                      cgggTGCTGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGATATGAAGTTAAAAAGCTGGAACGCACGCGCATCTTGAACGTTCGCTTACGCGGCATTCCGGTGGGGTAATGGCGCTATTTAACCGACGATGAGTTAATc                                                                                                                              <  1:4012472/148‑1 (MQ=255)
                      cTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATc                                                                                                                              <  2:766975/150‑1 (MQ=255)
                       tGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCg                                                                                                                             >  1:3947705/1‑150 (MQ=255)
                          tGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGAcc                                                                                                                          >  1:3652457/1‑150 (MQ=255)
                             aGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGACTGGCGCTATTTAACCGACGATGAGTTAATCGACctct                                                                                                                       >  2:3339838/1‑150 (MQ=255)
                             aGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACctct                                                                                                                       >  1:531618/1‑150 (MQ=255)
                             aGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACctct                                                                                                                       >  2:1455879/1‑150 (MQ=255)
                             aGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACctct                                                                                                                       >  1:2211482/1‑150 (MQ=255)
                             aGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACctct                                                                                                                       >  1:4766280/1‑150 (MQ=255)
                              ggggCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGACCGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCtt                                                                                                                      <  2:88773/150‑1 (MQ=255)
                              ggggCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCtt                                                                                                                      <  2:4289005/150‑1 (MQ=255)
                              ggggCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCtt                                                                                                                      <  1:235598/150‑1 (MQ=255)
                               gggCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCttt                                                                                                                     >  1:4105407/1‑150 (MQ=255)
                                    taaCCGTCAGATCCGGCGCATGTGCTAGAATTCCGGATATTAAGTCAAAAAGCTGGAACGCACGCGAATCATGACCGTTAGCTTACGCGGCATTCCGCTGGGGTAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCt                                                                                                                <  1:3202887/149‑1 (MQ=255)
                                    gAACCGTCAGATCCGGCGCATGTGCTAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCt                                                                                                                <  1:466363/150‑1 (MQ=255)
                                    gAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCt                                                                                                                <  2:2158245/150‑1 (MQ=255)
                                    gAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCt                                                                                                                <  2:1036509/150‑1 (MQ=255)
                                    gAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCAGTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCt                                                                                                                >  2:3913102/1‑150 (MQ=255)
                                     aaCCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTc                                                                                                               >  1:74958/1‑150 (MQ=255)
                                     aaCCGTCAGAGCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTACCCGACGATGAGTTAATCGACCTCTTTAAGCTc                                                                                                               <  1:156954/150‑1 (MQ=255)
                                          tCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGa                                                                                                          <  1:4327720/150‑1 (MQ=255)
                                               tCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAAtt                                                                                                     >  2:1639129/1‑150 (MQ=255)
                                                    cgcATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTACTCGACCTCTGTAAGCTCAGTGAAAATTCctct                                                                                                >  2:4096029/1‑150 (MQ=255)
                                                     gcATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCtt                                                                                               >  2:4072106/1‑150 (MQ=255)
                                                       aTGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTcc                                                                                             >  1:3537658/1‑150 (MQ=255)
                                                        tgtgCGAGCATTTCGGCTATGATGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCAGTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCg                                                                                            <  1:1780793/150‑1 (MQ=255)
                                                              aGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTaa                                                                                      <  2:381163/150‑1 (MQ=255)
                                                                    tCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCGGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTa                                                                                <  1:3396156/150‑1 (MQ=255)
                                                                      ggCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTaaa                                                                              <  2:3691398/150‑1 (MQ=255)
                                                                         tATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCg                                                                           <  1:1735878/150‑1 (MQ=255)
                                                                             aaGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGGTGGGGTATTGGCGATATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAgg                                                                       <  1:3203993/150‑1 (MQ=255)
                                                                               gTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGcc                                                                     <  2:2707660/150‑1 (MQ=255)
                                                                                 gAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCaa                                                                   >  1:1975264/1‑150 (MQ=255)
                                                                                 gAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCaa                                                                   >  1:3617330/1‑150 (MQ=255)
                                                                                 gAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCaa                                                                   >  2:1273749/1‑150 (MQ=255)
                                                                                 gAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCaa                                                                   >  1:3617285/1‑150 (MQ=255)
                                                                                    aaaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAAcc                                                                >  1:1298875/1‑150 (MQ=255)
                                                                                     aaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCg                                                               >  1:4300381/1‑150 (MQ=255)
                                                                                         tGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGaaaa                                                           >  2:4225964/1‑150 (MQ=255)
                                                                                         tGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGaaaa                                                           <  2:4744820/150‑1 (MQ=255)
                                                                                          ttaaggcacgcGAATCATTATGTTTAGATTAAGCGTCTTTCCGATGGGGTATTGGCGCTATTTAACCGACTATGAGTTAATCGACATCTTTAATCTCATTTAAAATTCCTCTTCCGTGGTAAAACCTAAAGCGAAGGCCAAACCGAAAAc                                                          <  1:2459119/145‑1 (MQ=255)
                                                                                          ggAACGCACGCGCATCTTGAACGTTAGCTTAAGCGTCATTCCGCTGGGGTATTGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAAc                                                          <  2:813868/150‑1 (MQ=255)
                                                                                          ggAACGCACGCGAATATTGAACTTTAGCTTAAGCGGCATTCCGCTGGGGGATTGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAAc                                                          <  1:2459163/150‑1 (MQ=255)
                                                                                               gcacgcGCATCATGAACGTTAGATTAAGCGGAATTCCGCTGGGGTAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCgg                                                     <  1:2068205/150‑1 (MQ=255)
                                                                                                cacgcGAATCATGAACGTTATCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCggg                                                    <  1:3545661/150‑1 (MQ=255)
                                                                                                 acgcGAATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGc                                                   <  1:3744728/150‑1 (MQ=255)
                                                                                                   gcgcATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGATTGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCAt                                                 <  2:869184/150‑1 (MQ=255)
                                                                                                     gcATCATGAACGTTAGCTTAAGCGGCAATCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGACAACAGCGGGCATCa                                               >  1:2265123/1‑150 (MQ=255)
                                                                                                       atcatGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCaaa                                             >  1:25028/1‑150 (MQ=255)
                                                                                                        tcatGAACTTTAGCTTAAGGGGCTTGCCGCTGGGGGATTGGCGCTATTAACCCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAAc                                            <  1:460467/150‑1 (MQ=255)
                                                                                                          atGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGt                                          >  2:4630272/1‑150 (MQ=255)
                                                                                                                gTTAGCTTACGCGGCATGCCGCTGGGGTATTGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTc                                    <  2:3354863/150‑1 (MQ=255)
                                                                                                                gTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTc                                    >  2:4724568/1‑150 (MQ=255)
                                                                                                                 ttcgCTTACGCGGCTTTCCGTTGGGGGATTGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCg                                   <  1:1076773/147‑1 (MQ=255)
                                                                                                                  tAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGt                                  >  1:1040956/1‑150 (MQ=255)
                                                                                                                  tAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGt                                  >  2:3830340/1‑150 (MQ=255)
                                                                                                                   aGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGtt                                 <  2:2270015/150‑1 (MQ=255)
                                                                                                                   aGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGtt                                 >  2:4399172/1‑150 (MQ=255)
                                                                                                                     cTTAAGCGGCATTCCGGTGGGGGAATGGCGATATTAAACCGACTATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTaa                               <  1:3318477/150‑1 (MQ=255)
                                                                                                                     cTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTaa                               >  1:1226949/1‑150 (MQ=255)
                                                                                                                     cTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTaa                               <  2:3734894/150‑1 (MQ=255)
                                                                                                                      ttACGCGGAATTCCGGTGGGGGAAGGGCGCGATTAAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAg                              <  1:4170783/150‑1 (MQ=255)
                                                                                                                       tAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGa                             >  2:547722/1‑150 (MQ=255)
                                                                                                                        aaGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGAt                            >  1:2749899/1‑150 (MQ=255)
                                                                                                                         aGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATg                           <  1:3188630/150‑1 (MQ=255)
                                                                                                                         aGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATg                           >  1:2329171/1‑150 (MQ=255)
                                                                                                                            ggaaTTCCGCTGGGGTATTGGCGATATTCACCCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTACCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaa                        <  1:4446389/147‑1 (MQ=255)
                                                                                                                              cATTCCGCTGGGGTAATGGCGCTATTAAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCAAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaaaa                      <  1:997148/150‑1 (MQ=255)
                                                                                                                               aTTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaaaaa                     >  2:1667316/1‑150 (MQ=255)
                                                                                                                                   cGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAACGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcgg                 <  2:2620471/150‑1 (MQ=255)
                                                                                                                                   cGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcgg                 >  2:1906004/1‑150 (MQ=255)
                                                                                                                                     cTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAg                              <  1:4609682/135‑1 (MQ=255)
                                                                                                                                     cTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAg                              >  2:4609682/1‑135 (MQ=255)
                                                                                                                                     cTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcggcg               >  1:2262384/1‑150 (MQ=255)
                                                                                                                                     cTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcggcg               >  2:2037024/1‑150 (MQ=255)
                                                                                                                                      tGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcggcgg              <  2:4156875/150‑1 (MQ=255)
                                                                                                                                      tGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcggcgg              >  2:2830736/1‑150 (MQ=255)
                                                                                                                                          gtattGGCGTTATTAACCCTACGTTTAGTTAATCGACCTATTTAAGCTATTTTAAATTTCCTCTTCCGTGTAAAAACCTAACGCGAAGGCAAACCCGAAAACAGCGGGAATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGaaaa          <  1:1648546/146‑1 (MQ=255)
                                                                                                                                            aaTGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTATGCTGGAAAAAACGGCGGaggagg        >  2:4108276/1‑145 (MQ=255)
                                                                                                                                              tGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGc      >  2:1314991/1‑150 (MQ=255)
                                                                                                                                               ggCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCg     <  2:2584253/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTC  >  CP009273/4220800‑4221095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑