BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,297,485	G→C	39.4%	G44G (GGC→GGG)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,297,485	0	G	C	39.4%	309.2 / inf	302	G44G (GGC→GGG)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand): ref base G (96/87); new base C (58/61); total (154/148)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 310 total aligned reads displayed.

CGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTT  >  CP009273/4297338‑4297634

 gccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCg                                                                                                                                                     <  1:118050/150‑1 (MQ=255)

  ccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCgg                                                                                                                                                    >  2:1335318/1‑150 (MQ=255)

   cgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCggg                                                                                                                                                   <  2:577825/150‑1 (MQ=255)

         tAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAAt                                                                                                                                             >  1:3948611/1‑150 (MQ=255)

            cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGAc                                                                                                                                          >  2:4381159/1‑150 (MQ=255)

             ggCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACt                                                                                                                                         >  1:4971/1‑150 (MQ=255)

                aGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCt                                                                                                                                      <  2:1610224/150‑1 (MQ=255)

                aGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCt                                                                                                                                      <  1:2873126/150‑1 (MQ=255)

                  gTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGc                                                                                                                                    >  2:2127379/1‑150 (MQ=255)

                   ttAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCg                                                                                                                                   >  2:864008/1‑150 (MQ=255)

                        cGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCg                                                                                                                              >  1:2401606/1‑150 (MQ=255)

                         gTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGc                                                                                                                             >  2:4418/1‑150 (MQ=255)

                          tAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑t                                                                                                                           <  1:3362887/150‑1 (MQ=255)

                           aGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑tt                                                                                                                          <  1:1081793/150‑1 (MQ=255)

                           aGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑tt                                                                                                                          >  1:4468086/1‑150 (MQ=255)

                             aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                        >  2:3182440/1‑150 (MQ=255)

                             aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                        >  2:1133777/1‑150 (MQ=255)

                              aataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttt                                                                                                                       <  2:2244958/150‑1 (MQ=255)

                                  atGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCg                                                                                                                   >  2:2819660/1‑150 (MQ=255)

                                    ggTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCa                                                                                                                 <  2:2074437/150‑1 (MQ=255)

                                      tGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAgt                                                                                                               <  2:2571382/150‑1 (MQ=255)

                                         gTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTa                                                                                                            >  2:4686084/1‑150 (MQ=255)

                                           ttATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGc                                                                                                          <  1:2751926/150‑1 (MQ=255)

                                             aTCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGt                                                                                                        >  2:4567596/1‑150 (MQ=255)

                                               cTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGttt                                                                                                      <  2:2464217/150‑1 (MQ=255)

                                               cTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGttt                                                                                                      >  2:4198376/1‑150 (MQ=255)

                                               cTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGttt                                                                                                      >  1:2825710/1‑150 (MQ=255)

                                                tttACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTc                                                                                                     >  2:3109818/1‑150 (MQ=255)

                                                  tACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCAc                                                                                                   >  1:104681/1‑150 (MQ=255)

                                                   aCTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCAcc                                                                                                  >  2:1581154/1‑150 (MQ=255)

                                                    cTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCt                                                                                                 >  2:1962837/1‑150 (MQ=255)

                                                     tGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCtt                                                                                                >  1:4325340/1‑150 (MQ=255)

                                                     tGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCtt                                                                                                <  1:4691762/150‑1 (MQ=255)

                                                       aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTct                                                                                              <  1:1297139/150‑1 (MQ=255)

                                                        ccAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTctg                                                                                             >  2:1696196/1‑150 (MQ=255)

                                                           gcgcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTctgctg                                                                                          <  1:4160664/150‑1 (MQ=255)

                                                               cGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCg                                                                                      >  1:1505402/1‑150 (MQ=255)

                                                               cGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCg                                                                                      >  1:3912138/1‑150 (MQ=255)

                                                                 gAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCa                                                                                    >  1:1697834/1‑150 (MQ=255)

                                                                  aaaTCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAg                                                                                   >  1:876557/1‑150 (MQ=255)

                                                                  aaaTCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAg                                                                                   >  2:1489267/1‑150 (MQ=255)

                                                                    aTCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGAc                                                                                 <  1:4266655/150‑1 (MQ=255)

                                                                    aTCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTAGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGAc                                                                                 >  2:2030123/1‑150 (MQ=255)

                                                                     tCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACa                                                                                <  1:1913900/150‑1 (MQ=255)

                                                                       ccATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACaaa                                                                              <  1:4751733/150‑1 (MQ=255)

                                                                         aTCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAg                                                                            >  2:934672/1‑150 (MQ=255)

                                                                           cACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCg                                                                          <  1:2652467/150‑1 (MQ=255)

                                                                           cACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCg                                                                          <  1:1422239/150‑1 (MQ=255)

                                                                            aCCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCgg                                                                         >  1:3059599/1‑150 (MQ=255)

                                                                            aCCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCAGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCgg                                                                         >  1:3644418/1‑150 (MQ=255)

                                                                                     tGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGtt                                                                >  1:2015746/1‑150 (MQ=255)

                                                                                      gACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTg                                                               >  1:3245082/1‑150 (MQ=255)

                                                                                        cAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACCAAAGCGGATATGCGTTGc                                                              >  1:214638/1‑150 (MQ=255)

                                                                                         agcgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGccc                                                            >  1:256470/1‑150 (MQ=255)

                                                                                            gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCa                                                         >  2:4780658/1‑150 (MQ=255)

                                                                                                 ttAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATcc                                                    <  2:720978/150‑1 (MQ=255)

                                                                                                  tAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCa                                                   <  1:1746794/150‑1 (MQ=255)

                                                                                                  tAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCa                                                   >  1:2089662/1‑150 (MQ=255)

                                                                                                   aaaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCAc                                                  <  2:2408025/150‑1 (MQ=255)

                                                                                                   aaaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCAc                                                  <  1:654118/150‑1 (MQ=255)

                                                                                                   aaaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCAc                                                  >  2:3997832/1‑150 (MQ=255)

                                                                                                    aaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACg                                                 >  1:2671244/1‑150 (MQ=255)

                                                                                                     aGCGCCTGAGTTGCTCGTCAATCATGTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGc                                                <  2:1878799/150‑1 (MQ=255)

                                                                                                      gcgcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGcc                                               <  2:4300485/150‑1 (MQ=255)

                                                                                                         ccTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGc                                            >  2:3477969/1‑150 (MQ=255)

                                                                                                          cTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCt                                           >  2:3845352/1‑150 (MQ=255)

                                                                                                          cTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCt                                           <  1:1622605/150‑1 (MQ=255)

                                                                                                             aGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacg                                        <  1:1869227/150‑1 (MQ=255)

                                                                                                               ttGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgac                                      >  2:2708580/1‑150 (MQ=255)

                                                                                                                 gCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGt                                    <  1:3826001/150‑1 (MQ=255)

                                                                                                                  cTCGTCAATCATTTCGCCGATACCCGACCCCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGtt                                   <  2:1354095/150‑1 (MQ=255)

                                                                                                                   tCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTa                                  >  1:3892535/1‑150 (MQ=255)

                                                                                                                   tCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTa                                  <  2:305300/150‑1 (MQ=255)

                                                                                                                   tCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTa                                  >  2:2649551/1‑150 (MQ=255)

                                                                                                                   tCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTa                                  <  2:2699964/150‑1 (MQ=255)

                                                                                                                    cGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTAt                                 <  2:1958276/150‑1 (MQ=255)

                                                                                                                       cAATCATTTCGCCGATACCCGACACCAGGCAGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCt                              <  2:3644418/150‑1 (MQ=255)

                                                                                                                        aaTCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAATCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTg                             >  1:2463620/1‑150 (MQ=255)

                                                                                                                              ttCGCCGATACCCGACACCAGGCCGGGAGCACTGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTg                       <  2:3418955/150‑1 (MQ=255)

                                                                                                                               tCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGc                      <  2:1505402/150‑1 (MQ=255)

                                                                                                                               tCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGc                      <  2:3912138/150‑1 (MQ=255)

                                                                                                                               tCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGc                      <  1:4060458/150‑1 (MQ=255)

                                                                                                                                 gCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCAt                    <  2:889325/150‑1 (MQ=255)

                                                                                                                                 gCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCAt                    >  1:3184785/1‑150 (MQ=255)

                                                                                                                                  ccGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATg                   <  2:397036/150‑1 (MQ=255)

                                                                                                                                   cGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGa                  <  1:4657988/150‑1 (MQ=255)

                                                                                                                                    gATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAg                 <  1:3442681/150‑1 (MQ=255)

                                                                                                                                     aTACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAgg                <  2:2349273/150‑1 (MQ=255)

                                                                                                                                     aTACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGGTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAgg                >  1:132434/1‑150 (MQ=255)

                                                                                                                                       aCCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCt              <  1:2377779/150‑1 (MQ=255)

                                                                                                                                        cccGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTTCATGAGGCTg             >  1:2182390/1‑150 (MQ=255)

                                                                                                                                        cccGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTg             >  1:2182496/1‑150 (MQ=255)

                                                                                                                                            acacCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTAcc         <  2:1443991/150‑1 (MQ=255)

                                                                                                                                               ccAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCg      >  1:2698363/1‑150 (MQ=255)

                                                                                                                                               ccAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCg      <  1:3716387/150‑1 (MQ=255)

                                                                                                                                                cAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGc     >  2:4434342/1‑150 (MQ=255)

                                                                                                                                                cAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGc     >  2:1705759/1‑150 (MQ=255)

                                                                                                                                                 aGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCt    >  1:1924983/1‑150 (MQ=255)

                                                                                                                                                  ggCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCCTATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCtt   >  1:2945038/1‑150 (MQ=255)

                                                                                                                                                  ggCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGATGCTGTACCTCGCtt   >  1:4430565/1‑150 (MQ=255)

CGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTT  >  CP009273/4297338‑4297634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`