Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,355,606 G→T 5.6% H329N (CAT→AAT)  dcuA ← C4‑dicarboxylate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,355,6060GT 5.6% 1309.1 / 32.7 337H329N (CAT→AAT) dcuAC4‑dicarboxylate antiporter
Reads supporting (aligned to +/- strand):  ref base G (138/180);  new base T (9/10);  total (147/190)
Fisher's exact test for biased strand distribution p-value = 8.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 340 total aligned reads displayed.

CAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGA  >  CP009273/4355457‑4355755
                                                                                                                                                     |                                                                                                                                                     
cagaccagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATg                                                                                                                                                       <  2:3414876/150‑1 (MQ=255)
  gaccagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGAc                                                                                                                                                     <  2:1265999/150‑1 (MQ=255)
     cagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCt                                                                                                                                                  >  2:2233140/1‑150 (MQ=255)
      agacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTg                                                                                                                                                 <  2:4498385/150‑1 (MQ=255)
      agacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTg                                                                                                                                                 <  1:2261029/150‑1 (MQ=255)
          acCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATTACCCTGAATc                                                                                                                                             >  1:2071582/1‑150 (MQ=255)
             gCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACt                                                                                                                                          <  2:3396833/150‑1 (MQ=255)
                  gAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCAcc                                                                                                                                     >  2:863954/1‑150 (MQ=255)
                  gAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCAcc                                                                                                                                     >  2:651848/1‑150 (MQ=255)
                    aagaagCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAg                                                                                                                                   >  1:2505878/1‑150 (MQ=255)
                     agaagCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGc                                                                                                                                  >  1:159285/1‑150 (MQ=255)
                     agaagCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGc                                                                                                                                  >  1:3100581/1‑150 (MQ=255)
                       aagCACCAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCgg                                                                                                                                <  2:552867/150‑1 (MQ=255)
                        agCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCACAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGt                                                                                                                               >  2:2511528/1‑150 (MQ=255)
                         gCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTa                                                                                                                              >  2:4710032/1‑150 (MQ=255)
                         gCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTa                                                                                                                              <  2:2098048/150‑1 (MQ=255)
                          cAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTAt                                                                                                                             >  1:4753140/1‑150 (MQ=255)
                                cggtcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGa                                                                                                                       <  1:4188417/150‑1 (MQ=255)
                                 ggtcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGAt                                                                                                                      <  2:2056275/150‑1 (MQ=255)
                                 ggtcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGAt                                                                                                                      <  2:3758994/150‑1 (MQ=255)
                                  gtcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGACCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATc                                                                                                                     >  2:729690/1‑150 (MQ=255)
                                   tcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATcc                                                                                                                    >  1:274636/1‑150 (MQ=255)
                                    cagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCa                                                                                                                   >  1:3124577/1‑150 (MQ=255)
                                    cagaggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCa                                                                                                                   <  1:4093434/150‑1 (MQ=255)
                                     agcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAg                                                                                                                  >  2:1531223/1‑150 (MQ=255)
                                        ggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCg                                                                                                               <  2:835337/150‑1 (MQ=255)
                                        ggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCg                                                                                                               <  2:1058457/150‑1 (MQ=255)
                                          tGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGAt                                                                                                             >  1:3331371/1‑150 (MQ=255)
                                           gAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGAtg                                                                                                            <  2:319528/150‑1 (MQ=255)
                                            aaaCGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGAtgt                                                                                                           <  1:1147234/150‑1 (MQ=255)
                                              aCGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGAtgttg                                                                                                         <  1:3419720/150‑1 (MQ=255)
                                                gTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATgttgtt                                                                                                       >  1:3003389/1‑150 (MQ=255)
                                                gTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATgttgtt                                                                                                       <  2:3974518/150‑1 (MQ=255)
                                                 ttCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGttgttg                                                                                                      <  1:3252164/150‑1 (MQ=255)
                                                    aGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGaa                                                                                                   <  1:4486033/150‑1 (MQ=255)
                                                      tGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATTACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAAc                                                                                                 <  1:3910242/150‑1 (MQ=255)
                                                      tGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAAc                                                                                                 <  2:3456510/150‑1 (MQ=255)
                                                        ccAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATTACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGa                                                                                               >  2:1377767/1‑150 (MQ=255)
                                                            agCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGt                                                                                           >  2:2288185/1‑150 (MQ=255)
                                                              ccATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTAt                                                                                         <  1:3597049/150‑1 (MQ=255)
                                                               cATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAACGTATc                                                                                        >  2:1101382/1‑150 (MQ=255)
                                                                aTCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCg                                                                                       >  2:2328120/1‑150 (MQ=255)
                                                                 tCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGc                                                                                      >  2:3131311/1‑150 (MQ=255)
                                                                   ggCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGccc                                                                                    >  2:327936/1‑150 (MQ=255)
                                                                       tCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGcc                                                                                <  1:671764/150‑1 (MQ=255)
                                                                        cAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCa                                                                               >  2:509684/1‑150 (MQ=255)
                                                                        cAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCa                                                                               >  1:1114228/1‑150 (MQ=255)
                                                                         aGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCAc                                                                              >  2:2884240/1‑150 (MQ=255)
                                                                          gTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACg                                                                             >  2:4179460/1‑150 (MQ=255)
                                                                              ttttGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAac                                                                         <  1:866798/150‑1 (MQ=255)
                                                                               tttGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAaca                                                                        >  1:987199/1‑150 (MQ=255)
                                                                               tttGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAaca                                                                        <  2:4038061/150‑1 (MQ=255)
                                                                               tttGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAaca                                                                        <  1:4252050/150‑1 (MQ=255)
                                                                               tttGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAaca                                                                        <  2:1809323/150‑1 (MQ=255)
                                                                                         cAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATAc                                                              >  2:426498/1‑150 (MQ=255)
                                                                                              tGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATTACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATa                                                         >  1:2947177/1‑150 (MQ=255)
                                                                                              tGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATa                                                         >  2:4441514/1‑150 (MQ=255)
                                                                                               gagaGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCATCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATAc                                                        <  2:3242317/150‑1 (MQ=255)
                                                                                                 gagTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAg                                                      >  2:1690504/1‑150 (MQ=255)
                                                                                                 gagTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAg                                                      >  2:3068441/1‑150 (MQ=255)
                                                                                                 gagTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAg                                                      >  1:1797469/1‑150 (MQ=255)
                                                                                                 gagTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGGTGGAAACGAAAGTATCGCCCAGCCACGCCACACCCAGGATACAAATACAg                                                      >  1:1363069/1‑150 (MQ=255)
                                                                                                   gTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgc                                                    <  1:941862/150‑1 (MQ=255)
                                                                                                   gTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgc                                                    <  1:1333097/150‑1 (MQ=255)
                                                                                                     aCAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgcgc                                                  <  1:2850000/150‑1 (MQ=255)
                                                                                                         cagagcagaAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCAt                                              <  2:4753140/150‑1 (MQ=255)
                                                                                                         cagagcagaAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCAt                                              <  2:1185951/150‑1 (MQ=255)
                                                                                                         cagagcagaAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCAt                                              <  1:3938099/150‑1 (MQ=255)
                                                                                                          agagcagaAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATa                                             <  1:604893/150‑1 (MQ=255)
                                                                                                          agagcagaAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATa                                             <  1:604458/150‑1 (MQ=255)
                                                                                                           gagcagaAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAc                                            <  2:797824/150‑1 (MQ=255)
                                                                                                            agcagaAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAcc                                           <  1:4637200/150‑1 (MQ=255)
                                                                                                               agaAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGc                                        <  1:4209830/150‑1 (MQ=255)
                                                                                                                 aaGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCtt                                      <  1:748487/150‑1 (MQ=255)
                                                                                                                  aGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATTACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCttt                                     >  1:3498054/1‑150 (MQ=255)
                                                                                                                        gaagaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGt                               <  2:3431162/150‑1 (MQ=255)
                                                                                                                            aagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTg                           >  2:4630944/1‑150 (MQ=255)
                                                                                                                            aagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTg                           >  1:4300536/1‑150 (MQ=255)
                                                                                                                             agaTGACGGCCAGCAGCCACGGATGACCCTGAATCTCTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATCCAGGCGCTCATACCTGCTTTGAAGGTGCTgg                          <  2:4344966/150‑1 (MQ=255)
                                                                                                                               aTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAg                        >  2:2758306/1‑150 (MQ=255)
                                                                                                                               aTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAg                        <  1:4751291/150‑1 (MQ=255)
                                                                                                                                    ggCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAg                   <  2:903490/150‑1 (MQ=255)
                                                                                                                                     gCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAgg                  >  2:613213/1‑150 (MQ=255)
                                                                                                                                     gCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAgg                  >  2:2556763/1‑150 (MQ=255)
                                                                                                                                       cagcagCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAt                <  1:1577737/150‑1 (MQ=255)
                                                                                                                                        agcagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtg               >  2:4083688/1‑150 (MQ=255)
                                                                                                                                         gcagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtgt              <  2:2940755/150‑1 (MQ=255)
                                                                                                                                           agcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtgttg            <  1:2569307/150‑1 (MQ=255)
                                                                                                                                           agcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtgttg            <  1:1243457/150‑1 (MQ=255)
                                                                                                                                            gcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGAtgttgt           <  1:888541/150‑1 (MQ=255)
                                                                                                                                             ccACGGATTACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTc          <  1:1423227/150‑1 (MQ=255)
                                                                                                                                               aCGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCgg        <  2:4726458/150‑1 (MQ=255)
                                                                                                                                                 ggATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTa      >  1:1967107/1‑150 (MQ=255)
                                                                                                                                                 ggATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTa      <  1:1276304/150‑1 (MQ=255)
                                                                                                                                                  gATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGTGGATGTTTTCGGTAt     >  1:4403161/1‑150 (MQ=255)
                                                                                                                                                  gATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGtgt     <  1:3197191/150‑3 (MQ=255)
                                                                                                                                                   aTGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATc    >  2:1295567/1‑150 (MQ=255)
                                                                                                                                                   aTGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATc    >  2:360635/1‑150 (MQ=255)
                                                                                                                                                    tGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCg   >  2:19808/1‑150 (MQ=255)
                                                                                                                                                    tGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCg   <  1:154699/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGA  >  CP009273/4355457‑4355755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑