Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,382,399 A→C 5.8% intergenic (+35/‑1) glyX → / → glyY tRNA‑Gly/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,382,3990AC 5.8% 428.4 / 9.0 162intergenic (+35/‑1)glyX/glyYtRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (107/42);  new base C (4/7);  total (112/50)
Fisher's exact test for biased strand distribution p-value = 3.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

CAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTC  >  CP009273/4382251‑4382548
                                                                                                                                                     |                                                                                                                                                     
cAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTT‑AAAGTGCTGAAAGGCACAGACCACCCAAg                                                                                                                                                      >  1:2300500/1‑150 (MQ=255)
cAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTT‑AAAGTGATTTAATGAACAAACCCCCCCAg                                                                                                                                                      >  2:1940157/1‑150 (MQ=14)
   aaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AACGTGCTGTAAGGACCAGACCACCCAAGCgg                                                                                                                                                   >  2:3509426/1‑150 (MQ=255)
      ttGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGaa                                                                                                                                                <  1:2569829/150‑1 (MQ=255)
       tGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGGGCTGTAAGGCCCAGACCACCCACGCGGGAAt                                                                                                                                               >  2:2409224/1‑150 (MQ=255)
            aGTGCTGCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTc                                                                                                                                          <  2:1506304/150‑1 (MQ=255)
               gctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTTTG‑AAAGTGCTGAAAGGCACACACCACCCAAGAGGGAAAAGCTCAGt                                                                                                                                       >  2:1255197/1‑150 (MQ=17)
                 tgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATG‑AAAGAGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTg                                                                                                                                     >  2:3285478/1‑150 (MQ=255)
                   cAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGt                                                                                                                                   >  1:1012282/1‑150 (MQ=255)
                   cAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATG‑AAAGTGCTGTAAGGCACAGACCACCCCAGCGGGAATAGCGCCGTTGGt                                                                                                                                   >  2:946204/1‑150 (MQ=255)
                          cttaacaccCCTGCGGGAATAGCTCAGTTGGTAGATCACGAACTTGCCAAGGTCGGGTTCGCGAGTTCGAGTCTAGTTTCACGCTCCAAAATTTG‑AAAGTTCTGTAAGGCACAGACCACCCTAGCGGGAATAGCTCAGTTGGTAGAGCAc                                                                                                                            <  1:580444/145‑1 (MQ=14)
                          caGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCAc                                                                                                                            <  1:3915198/150‑1 (MQ=255)
                            gACCACCCAAGCGGGAATAGCTCAGTTGTTAGAGCACGACCTTGCAAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCTCTCCAAAATTTG‑AAAGTGCTGTAAGGCAAAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGa                                                                                                                          <  1:2447751/150‑1 (MQ=255)
                             accaccCAAGCGGGACTAGCTCAGTTGGTCGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCGGTAAGGCACAGACCACCCACGCGGGCATAGCTCAGTTGGTAGAGCACGAc                                                                                                                         >  2:4046117/1‑150 (MQ=14)
                             accaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATCTT‑AAAGATCTCTAAGGCACAGACCACCCAACAGGGAATAGCACAGTTGGTAGAGCACGAc                                                                                                                         >  2:3435124/1‑150 (MQ=14)
                              ccaccCCCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACTGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGAcc                                                                                                                        <  2:17050/150‑1 (MQ=255)
                               caccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTT‑AAAGTGCTGTAATTAACAGACCACCCAAGCGGGAATAGCTCAGTTGGTATAGCAAGAcca                                                                                                                       >  2:2797177/1‑149 (MQ=14)
                               caccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCaccaccg                                                                                                                       >  1:3662947/1‑149 (MQ=255)
                                 cccAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                                                                                     >  1:3521271/1‑150 (MQ=255)
                                   cAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc                                                                                                                   >  1:73137/1‑150 (MQ=40)
                                    aaGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                                  >  1:2712487/1‑150 (MQ=40)
                                    aaGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCa                                                                                                                  >  1:2297376/1‑150 (MQ=40)
                                      gCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACCACCTAGCCAAg                                                                                                                >  1:1906530/1‑150 (MQ=255)
                                       cGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCGCAGTTGGTAGAGCACGACCTTGCCaatg                                                                                                               >  2:3637689/1‑148 (MQ=255)
                                        gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATATCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                                                                                                              >  2:636209/1‑150 (MQ=38)
                                          gAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                            >  1:527058/1‑150 (MQ=38)
                                              aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGTCACAGACCACCCAAGCGGGAATATCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggga                                                                                                        >  2:2978192/1‑149 (MQ=255)
                                               gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACATTGCCAAGGTCGGGGTc                                                                                                       >  2:2588577/1‑150 (MQ=255)
                                                 tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTACGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                                                                                     >  1:1480390/1‑150 (MQ=38)
                                                      tGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGATGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                                >  1:1120493/1‑150 (MQ=38)
                                                        gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCg                                                                                              <  1:2626541/150‑1 (MQ=38)
                                                        gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCg                                                                                              <  2:1716405/150‑1 (MQ=38)
                                                          agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTG‑AAAGCGCTGTAATACCCACACCACCCCACCGGGAAAAGCAACGGTGCGACAGCCCGACCGTGCCCAGCTCGGGGTCCCGAGttaggg                                                                                            >  2:688313/1‑146 (MQ=2)
                                                          agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCGCCAAAATATG‑AAAGTGCTGTAAGGCCCAGACCACCCAAGCGGGAATAGCTCAGGTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAg                                                                                            >  2:3925266/1‑150 (MQ=255)
                                                            agCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtc                                                                                          >  1:2948581/1‑150 (MQ=38)
                                                              cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCACCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc                                                                                        <  1:1452440/150‑1 (MQ=38)
                                                               aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                                                       <  2:4109857/150‑1 (MQ=38)
                                                               aCGACCGGGCCCCGGGCGGGGGCGCGAGTTCGAGTCTCGTTTCCCGCTCCACCAGTTG‑ACAGTGCGGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTGGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                                                       <  2:3834067/150‑1 (MQ=11)
                                                                 gACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTTTAAGGCACAGACCACCCAATCGGGAATAGCTCAGTTGGTAGAGCACGACCTTTCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt                                                                                     >  1:3729919/1‑150 (MQ=255)
                                                                 gACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtg                                                                                     >  2:3871018/1‑149 (MQ=255)
                                                                   ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                                   >  1:2084517/1‑150 (MQ=38)
                                                                     ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTACAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                 <  2:3142153/150‑1 (MQ=38)
                                                                       gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGc                                                                               >  2:1168634/1‑150 (MQ=38)
                                                                       gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGAGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTGGGTAGAGCACGACCTTGCCAAGGTCGGGGACGCGAGTTCGAGTCTCGTTTCCCGc                                                                               >  2:4213119/1‑150 (MQ=255)
                                                                          aaGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc                                                                            >  2:3743736/1‑150 (MQ=38)
                                                                          aaGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTcc                                                                            >  2:1116220/1‑150 (MQ=38)
                                                                           aGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCACCa                                                                           >  1:3360104/1‑150 (MQ=38)
                                                                           aGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTGCGAGTCTCGTTTCCCGCTCCa                                                                           >  2:3060881/1‑150 (MQ=38)
                                                                           aGGTCGGGGTATCGTGTTGTAGTTACGTTACCCGCCCAAAATTTTG‑AAAGTGCTGCAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa                                                                           <  2:1212325/150‑1 (MQ=11)
                                                                               cGGGGTTGCGAGTTCTAGTCACTTTTCCCTCTCCAAACTTTT‑AAAGTGCTGTAAGGAACAGAACAACCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAtt                                                                       <  2:4554302/150‑1 (MQ=14)
                                                                                    tCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATtcttct                                                                  >  1:1298002/1‑150 (MQ=255)
                                                                                    tCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATtcttct                                                                  >  2:2723882/1‑150 (MQ=255)
                                                                                    tCGCGAGTTCGAGTCTCGTTTCCAGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTACCGCTCCAAATtcttct                                                                  >  1:1297640/1‑150 (MQ=255)
                                                                                      gctattgcGACTCTCGTTACCCGCTCCAAATTTTT‑AAAGTGCTGTAACTAACAGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctct                                                                <  1:941401/143‑1 (MQ=14)
                                                                                       cgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGTGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctctc                                                               >  1:2034799/1‑150 (MQ=255)
                                                                                       cgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTtctctc                                                               >  1:325588/1‑150 (MQ=255)
                                                                                        gAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCa                                                              >  1:3813118/1‑150 (MQ=255)
                                                                                           ttCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATa                                                           >  2:493582/1‑150 (MQ=255)
                                                                                             cGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATaa                                                          >  1:532254/1‑150 (MQ=255)
                                                                                                gTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAata                                                      >  2:2419649/1‑150 (MQ=255)
                                                                                                   tcGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATcc                                                   >  1:3130748/1‑150 (MQ=255)
                                                                                                   tcGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATcc                                                   >  1:636566/1‑150 (MQ=255)
                                                                                                    cGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCca                                                  >  1:3704170/1‑150 (MQ=255)
                                                                                                     gTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcac                                                 >  2:4626740/1‑150 (MQ=255)
                                                                                                     gTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcac                                                 >  2:1966807/1‑150 (MQ=255)
                                                                                                     gTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcac                                                 >  2:3224139/1‑150 (MQ=255)
                                                                                                     gTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcac                                                 >  2:1517678/1‑150 (MQ=255)
                                                                                                      tttCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCcaca                                                >  2:4588574/1‑150 (MQ=255)
                                                                                                       ttCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAg                                               >  2:1839095/1‑150 (MQ=255)
                                                                                                        tCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGc                                              >  1:1637322/1‑150 (MQ=255)
                                                                                                        tCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGc                                              >  1:1638114/1‑150 (MQ=255)
                                                                                                        tCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGc                                              >  2:2966009/1‑150 (MQ=255)
                                                                                                        tCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATACACAGc                                              >  1:4045864/1‑150 (MQ=255)
                                                                                                               ccAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGa                                       >  1:1593993/1‑150 (MQ=255)
                                                                                                               ccAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGa                                       >  2:231845/1‑150 (MQ=255)
                                                                                                                  aaaTTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGc                                    >  1:3558228/1‑150 (MQ=255)
                                                                                                                  aaaTTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGc                                    >  1:3557864/1‑150 (MQ=255)
                                                                                                                       tG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTAt                               <  2:532289/150‑1 (MQ=255)
                                                                                                                       tG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTAt                               >  2:1400089/1‑150 (MQ=255)
                                                                                                                        gaaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTAt                               <  2:532254/148‑1 (MQ=255)
                                                                                                                        g‑AATGTGCTGTTAGGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTAtt                              <  1:2785117/150‑1 (MQ=255)
                                                                                                                          aaaGTGCTGAAAGGCACAGACCACCCAAGCGGGAATAGCTCTGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCTTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTg                             <  1:410223/150‑1 (MQ=255)
                                                                                                                            aGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCt                           >  1:4336437/1‑150 (MQ=255)
                                                                                                                              tGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTgg                         >  1:4431891/1‑150 (MQ=255)
                                                                                                                              tGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTgg                         >  1:4431493/1‑150 (MQ=255)
                                                                                                                                  gTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGtttt                     <  2:73137/150‑1 (MQ=255)
                                                                                                                                  gTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGtttt                     >  1:1277168/1‑150 (MQ=255)
                                                                                                                                   tAAGGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCCCAGCGACGCGATGCGTTATTGCTGGttttt                    <  1:4046117/150‑1 (MQ=255)
                                                                                                                                   tAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGttttt                    <  1:3435124/150‑1 (MQ=255)
                                                                                                                                     aGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgt                  >  2:4414201/1‑150 (MQ=255)
                                                                                                                                      cgcactcaccaccCATGGGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgtt                 <  1:96605/143‑1 (MQ=255)
                                                                                                                                       gCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgttg                <  2:2712487/150‑1 (MQ=255)
                                                                                                                                       gCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgttg                >  2:1555301/1‑150 (MQ=255)
                                                                                                                                       gCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgttg                <  1:3927742/150‑1 (MQ=255)
                                                                                                                                            gACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTctct           <  2:2297376/150‑1 (MQ=255)
                                                                                                                                             accaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTg          >  2:4352079/1‑150 (MQ=255)
                                                                                                                                              ccaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCCCAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGa         <  1:2848107/150‑1 (MQ=255)
                                                                                                                                                accCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACa       >  1:4479674/1‑150 (MQ=255)
                                                                                                                                                accCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTGATTGCTGGTTTTTGTTGTCTCTGACa       >  1:4479143/1‑150 (MQ=255)
                                                                                                                                                     aGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAActc  >  1:808892/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTC  >  CP009273/4382251‑4382548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑