Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,402,850 G→T 39.6% L2L (CTG→CTT)  yjfC → ATP‑Grasp family ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,402,8500GT39.6% 295.2 / 310.7 263L2L (CTG→CTT) yjfCATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base G (90/69);  new base T (58/46);  total (148/115)
Fisher's exact test for biased strand distribution p-value = 9.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 269 total aligned reads displayed.

CTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACC  >  CP009273/4402703‑4402999
                                                                                                                                                   |                                                                                                                                                     
cTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTtag                                                                                                                                                     >  2:2587505/1‑150 (MQ=255)
 tGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTtaga                                                                                                                                                    >  2:2202549/1‑150 (MQ=255)
       ttACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAAc                                                                                                                                              >  1:1497043/1‑150 (MQ=255)
            ccTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTGAGACACAACGTTcc                                                                                                                                         >  2:2073344/1‑150 (MQ=255)
            ccTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTcc                                                                                                                                         <  2:4354057/150‑1 (MQ=255)
             cTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTACAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAAAGTTCCt                                                                                                                                        >  2:1201418/1‑150 (MQ=255)
              tGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCtg                                                                                                                                       <  1:562012/150‑1 (MQ=255)
              tGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCtg                                                                                                                                       <  2:1367978/150‑1 (MQ=255)
              tGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTACtg                                                                                                                                       >  1:2076900/1‑150 (MQ=255)
                 cgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGc                                                                                                                                    <  1:894566/150‑1 (MQ=255)
                  gcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGGGGGCCTTGGGGAGGCGAATCATGCTGAGACACAACGTTCCAGTGCg                                                                                                                                   >  1:2418450/1‑150 (MQ=255)
                   cTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCAGTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGa                                                                                                                                  >  1:4388866/1‑150 (MQ=255)
                      cGGTCCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATAATGCTGAGACCCAACCATACAGTGCGACgg                                                                                                                               >  1:3627845/1‑150 (MQ=255)
                       ggTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACggg                                                                                                                              <  1:1600473/150‑1 (MQ=255)
                       ggTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACggg                                                                                                                              <  2:359895/150‑1 (MQ=255)
                          tCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATc                                                                                                                           <  1:1863259/150‑1 (MQ=255)
                           cTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATGGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCt                                                                                                                          >  2:3272989/1‑150 (MQ=255)
                                   aaaGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGa                                                                                                                  >  2:3007493/1‑150 (MQ=255)
                                   aaaGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGa                                                                                                                  <  1:4580087/150‑1 (MQ=255)
                                    aaGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTACAGCGCCCGTGGGCATTTGGGAGGCTAATCATGCTGAGACACAACCTTCCTGTGCGCCGGGATCTGGACCAGAt                                                                                                                 >  1:2129445/1‑150 (MQ=255)
                                     agagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCAGTGGGGGGGCTAATCATGCTGAGACACCACGTTCCTGTGCGACGGGATCTGGACCAGAta                                                                                                                >  1:1299677/1‑149 (MQ=255)
                                      gagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATcg                                                                                                               >  2:3348952/1‑150 (MQ=255)
                                      gagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGATATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATcg                                                                                                               <  1:1024026/150‑1 (MQ=255)
                                      gagTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGATATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATcg                                                                                                               <  1:3424486/150‑1 (MQ=255)
                                            tACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTg                                                                                                         >  2:2522280/1‑150 (MQ=255)
                                             aCTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGa                                                                                                        <  1:4705498/150‑1 (MQ=255)
                                              ctctTCGGGCGGCTTTACCACCAAAAAAGCGTCGACCGTTTCTCGCGGCGGCTATGGTCGGTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGAc                                                                                                       <  2:1170190/150‑1 (MQ=255)
                                               tctTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCGTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACa                                                                                                      >  2:3495097/1‑150 (MQ=255)
                                                 ttCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAAc                                                                                                    <  1:3875061/150‑1 (MQ=255)
                                                 ttCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAAc                                                                                                    <  1:163870/150‑1 (MQ=255)
                                                    gggCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGt                                                                                                 <  2:696649/150‑1 (MQ=255)
                                                    gggCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGt                                                                                                 <  2:1544370/150‑1 (MQ=255)
                                                     ggcggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGtt                                                                                                >  2:3682146/1‑150 (MQ=255)
                                                      gcggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGttt                                                                                               <  1:285907/150‑1 (MQ=255)
                                                        ggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCg                                                                                             <  1:2224705/150‑1 (MQ=255)
                                                        ggcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCGTATACACAACGTTCCTGTGCGCCGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCg                                                                                             >  1:1774821/1‑150 (MQ=255)
                                                         gcTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCCGTGGGGAGGCTAATCATGCTTATACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACACCGGTTTCGa                                                                                            >  1:4162099/1‑150 (MQ=255)
                                                          cTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCCGCGCCCGTGGGCAGTGGGGAGGCTACTCATGCTTAGTCACAACGTTCCTGTGCGCCGGGATCTGGACCAGATCGCCGCTGACACCGGGTTCGag                                                                                           >  2:2019975/1‑149 (MQ=255)
                                                           tttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACt                                                                                          <  2:393386/150‑1 (MQ=255)
                                                            ttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACtt                                                                                         >  2:4770437/1‑150 (MQ=255)
                                                            ttACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTACAGCGCCCGTGGGCCTTGGGGGGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATAGCCGCTGCCAACGGTTTCGCCtt                                                                                         >  1:3237209/1‑150 (MQ=255)
                                                               ccaccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTAGACTTTCa                                                                                      >  1:3044626/1‑150 (MQ=255)
                                                                 accaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCata                                                                                    <  2:1432928/150‑1 (MQ=255)
                                                                 accaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCGTCCAGCGCCCGGGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCata                                                                                    >  1:2588903/1‑150 (MQ=255)
                                                                  ccaAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCatat                                                                                   <  1:607196/150‑1 (MQ=255)
                                                                         aGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGAc                                                                            >  2:4052510/1‑150 (MQ=255)
                                                                         aGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGAc                                                                            >  2:4585962/1‑150 (MQ=255)
                                                                         aGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGAc                                                                            <  1:28617/150‑1 (MQ=255)
                                                                         aGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGAc                                                                            <  1:28547/150‑1 (MQ=255)
                                                                         aGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGAc                                                                            <  2:2662572/150‑1 (MQ=255)
                                                                            gTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAAt                                                                         <  1:4667846/150‑1 (MQ=255)
                                                                            gTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAAt                                                                         >  2:1109294/1‑150 (MQ=255)
                                                                             tCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATACTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATg                                                                        >  1:722160/1‑150 (MQ=255)
                                                                              cTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACCAACCAGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGa                                                                       >  2:3985250/1‑150 (MQ=255)
                                                                               tACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa                                                                      >  1:4261175/1‑150 (MQ=255)
                                                                                aCCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaaa                                                                     >  2:2424740/1‑150 (MQ=255)
                                                                                 ccGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAAt                                                                    <  1:4356101/150‑1 (MQ=255)
                                                                                  cGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGGATTGGGGAGGCTAATAATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATc                                                                   >  2:3219377/1‑150 (MQ=255)
                                                                                  cGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTAATATCATCGACAATGAAAta                                                                   >  2:118398/1‑149 (MQ=255)
                                                                                    tttCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTa                                                                 <  2:4652327/150‑1 (MQ=255)
                                                                                     ttCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCCTGCTGAGACACAACGTTCCAGTGCGACGGGATCTGGACCAGATCGCCGCTGACCACGGTTTCGACTTTCATATCATCGACAATGAAATCTAt                                                                >  2:1302891/1‑150 (MQ=255)
                                                                                        tcGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTgg                                                             <  1:2687481/150‑1 (MQ=255)
                                                                                               ggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATgaga                                                      >  2:25562/1‑150 (MQ=255)
                                                                                                  tATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTc                                                   <  2:560350/150‑1 (MQ=255)
                                                                                                         gTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTa                                            <  1:3216026/150‑1 (MQ=255)
                                                                                                           tcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAcc                                          <  1:2519096/150‑1 (MQ=255)
                                                                                                            cttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCg                                         >  2:4546420/1‑150 (MQ=255)
                                                                                                               ccAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGttt                                      <  2:133545/150‑1 (MQ=255)
                                                                                                                cAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGtttt                                     >  2:4246508/1‑150 (MQ=255)
                                                                                                                cAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGtttt                                     >  2:1619812/1‑150 (MQ=255)
                                                                                                                cAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGtttt                                     >  2:3657424/1‑150 (MQ=255)
                                                                                                                 aGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCTACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCCTCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTa                                    <  1:4452215/150‑1 (MQ=255)
                                                                                                                     cccGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTActct                                >  1:4312097/1‑150 (MQ=255)
                                                                                                                     cccGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTActct                                >  2:1160238/1‑150 (MQ=255)
                                                                                                                        gTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTgcg                             <  2:1879234/150‑1 (MQ=255)
                                                                                                                           ggCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCa                          >  1:4744053/1‑150 (MQ=255)
                                                                                                                           ggCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCa                          >  1:2183526/1‑150 (MQ=255)
                                                                                                                           ggCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGACa                          >  2:4255322/1‑150 (MQ=255)
                                                                                                                            gCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAg                         >  2:2844982/1‑150 (MQ=255)
                                                                                                                             cATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGa                        >  1:972021/1‑150 (MQ=255)
                                                                                                                              aTTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGAt                       >  2:2739726/1‑150 (MQ=255)
                                                                                                                               ttGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGAtt                      >  1:1113958/1‑150 (MQ=255)
                                                                                                                                tGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTg                     >  2:1196514/1‑150 (MQ=255)
                                                                                                                                tGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTg                     <  1:2522307/150‑1 (MQ=255)
                                                                                                                                tGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTg                     >  2:1761439/1‑150 (MQ=255)
                                                                                                                                tGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTg                     >  1:3515899/1‑150 (MQ=255)
                                                                                                                                tGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTg                     >  1:2651098/1‑150 (MQ=255)
                                                                                                                                 ggggAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGa                    <  1:4693276/150‑1 (MQ=255)
                                                                                                                                   ggAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAag                  >  1:3383328/1‑150 (MQ=255)
                                                                                                                                    gAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAaga                 >  2:4547568/1‑150 (MQ=255)
                                                                                                                                     aGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAagag                <  1:1705517/150‑1 (MQ=255)
                                                                                                                                      ggCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGc               <  2:1934395/150‑1 (MQ=255)
                                                                                                                                          aaTCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGAt           >  1:1443668/1‑150 (MQ=255)
                                                                                                                                          aaTCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGAt           >  2:3545233/1‑150 (MQ=255)
                                                                                                                                           aTCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATc          <  1:3778585/150‑1 (MQ=255)
                                                                                                                                           aTCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATc          >  1:1944970/1‑150 (MQ=255)
                                                                                                                                           aTCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATc          <  2:1133995/150‑1 (MQ=255)
                                                                                                                                                  tGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGaacaac   >  2:4793198/1‑150 (MQ=255)
                                                                                                                                                   gagaCACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAAcc  >  2:4407959/1‑150 (MQ=255)
                                                                                                                                                   gagaCACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAAcc  <  2:1692446/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACC  >  CP009273/4402703‑4402999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑