BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,407,307	A→T	21.4%	H180L (CAC→CTC)	yjfP →	acyl CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,407,307	0	A	T	21.4%	873.1 / 251.7	374	H180L (CAC→CTC)	yjfP	acyl CoA esterase
Reads supporting (aligned to +/- strand): ref base A (174/120); new base T (44/36); total (218/156)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

Only 100 of 382 total aligned reads displayed.

GGTGAGATGTACCGCCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGC  >  CP009273/4407158‑4407456

ggTGAGATGTACCGCCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAacca                                                                                                                                                       <  2:3992481/150‑1 (MQ=255)

  tGAGATGTACCGCCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAacctcc                                                                                                                                                     >  2:847796/1‑150 (MQ=255)

       tGTACCGCCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGaa                                                                                                                                                >  1:555533/1‑150 (MQ=255)

         tACCGCCAGCTTGTTGGTTTGGGGTTTTTTTACATAAGGCGCCCTTTCTGTGTTTCCACGGCTTATACATTAAAGGGCAGCACAGCAGAATGAATTAAATAACATTGTCGCGCCACTGGCAGTGTGGTAAGCGACAAACCACCTGGaaca                                                                                                                                              <  1:4167972/150‑1 (MQ=255)

         tACCGCCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGaaca                                                                                                                                              <  2:4451163/150‑1 (MQ=255)

            cGCCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACt                                                                                                                                           <  2:1129217/150‑1 (MQ=255)

             gCCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACtt                                                                                                                                          >  1:3891656/1‑150 (MQ=255)

             gCCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACtt                                                                                                                                          >  2:4031408/1‑150 (MQ=255)

                aGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGt                                                                                                                                       >  1:141544/1‑150 (MQ=255)

                aGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGt                                                                                                                                       >  2:199906/1‑150 (MQ=255)

                  cATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGa                                                                                                                                     >  1:3199826/1‑150 (MQ=255)

                  cATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGa                                                                                                                                     <  2:1094919/150‑1 (MQ=255)

                  cATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGa                                                                                                                                     >  2:4354265/1‑150 (MQ=255)

                   atgttgGGATCGGGATATTTTACATCACTCGCCCGTTCACTGTTCCCACCGCGGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGAc                                                                                                                                    <  1:3325914/150‑1 (MQ=255)

                    tgatgGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACa                                                                                                                                   <  2:3473987/150‑1 (MQ=255)

                      atgGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGa                                                                                                                                 >  1:3681900/1‑150 (MQ=255)

                           aTCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACctct                                                                                                                            >  2:780874/1‑150 (MQ=255)

                             cGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTAc                                                                                                                          <  2:4193887/150‑1 (MQ=255)

                              gggCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTAct                                                                                                                         >  1:3780153/1‑150 (MQ=255)

                              gggATATTTTACACAACTCGCCGGTTCAGTTTTTCCACCGCTTATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTAct                                                                                                                         <  1:1643843/150‑1 (MQ=255)

                                   aTTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCtgt                                                                                                                    <  2:4332263/150‑1 (MQ=255)

                                   aTTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCtgt                                                                                                                    <  2:3530166/150‑1 (MQ=255)

                                   aTTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCtgt                                                                                                                    <  1:664880/150‑1 (MQ=255)

                                    ttttACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCtgtg                                                                                                                   <  2:184006/150‑1 (MQ=255)

                                    ttttACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCtgtg                                                                                                                   <  2:1586091/150‑1 (MQ=255)

                                     tttACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTgg                                                                                                                  <  2:1883338/150‑1 (MQ=255)

                                       tACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCa                                                                                                                >  1:3022164/1‑150 (MQ=255)

                                       tACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCa                                                                                                                >  2:4581464/1‑150 (MQ=255)

                                       tACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCa                                                                                                                >  2:3226346/1‑150 (MQ=255)

                                       tACATCACTCGCCCGTTCACTGTTTCCACAGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGATGTGGCa                                                                                                                >  2:2751994/1‑150 (MQ=255)

                                        aCATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGACGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCAt                                                                                                               >  2:4620181/1‑150 (MQ=255)

                                         cATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATg                                                                                                              <  1:2790918/150‑1 (MQ=255)

                                         cATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATg                                                                                                              >  1:3503488/1‑150 (MQ=255)

                                           tCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGc                                                                                                            >  1:3687389/1‑150 (MQ=255)

                                           tCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGc                                                                                                            <  2:3839906/150‑1 (MQ=255)

                                            cACTCGCCGTTTCCGTTTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGTGTGGGACGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGcc                                                                                                           <  1:16687/150‑1 (MQ=255)

                                                cGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTcga                                                                                                       >  2:1315576/1‑150 (MQ=255)

                                                    cGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGAt                                                                                                   <  1:1835059/150‑1 (MQ=255)

                                                    cGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGAt                                                                                                   >  1:4014797/1‑150 (MQ=255)

                                                     gTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATg                                                                                                  >  1:2530507/1‑150 (MQ=255)

                                                      ttCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGa                                                                                                 <  1:3093023/150‑1 (MQ=255)

                                                        cACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACg                                                                                               >  2:4661461/1‑150 (MQ=255)

                                                         aCTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGt                                                                                              >  2:3761579/1‑150 (MQ=255)

                                                         aCTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGt                                                                                              <  1:2159328/150‑1 (MQ=255)

                                                              ttCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgc                                                                                         >  1:3457437/1‑150 (MQ=255)

                                                               tCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgcc                                                                                        <  1:3956519/150‑1 (MQ=255)

                                                               tCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGAGGACGTTGtgcc                                                                                        >  2:3614771/1‑150 (MQ=255)

                                                               cccACCGCTTATACCTCAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgcc                                                                                        <  1:1233791/149‑1 (MQ=255)

                                                                ccACCGCTTATCCCGCACCCGGCAGCACAGCAGAATGAATTCAAAAACATGGTCGCGCCCCTGGCAGAGGGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgcct                                                                                       <  1:425301/150‑1 (MQ=255)

                                                                ccACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgcct                                                                                       <  1:199297/150‑1 (MQ=255)

                                                                 cACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGTGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgcctg                                                                                      >  2:700662/1‑150 (MQ=255)

                                                                  aCCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgcctgc                                                                                     >  1:1135392/1‑150 (MQ=255)

                                                                   ccGCTGATACCTGACACGGCAGCACAGCAGAATGAATACAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgcctgcc                                                                                    <  1:961740/150‑1 (MQ=255)

                                                                   ccGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgcctgcc                                                                                    <  2:2345501/150‑1 (MQ=255)

                                                                      cTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCcgac                                                                                 >  2:57775/1‑150 (MQ=255)

                                                                         aTACCTGACACGGCAGCACAGCATAATGAATTCAATAACTTTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGaa                                                                              <  1:24475/150‑1 (MQ=255)

                                                                         aTACCTGAAACGGCAGCACAGCAGAATGAATTCAATACCATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGaa                                                                              <  1:1943966/150‑1 (MQ=255)

                                                                           aCCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATc                                                                            >  2:4401322/1‑150 (MQ=255)

                                                                           aCCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATc                                                                            >  2:1761442/1‑150 (MQ=255)

                                                                              tGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACt                                                                         <  2:262946/150‑1 (MQ=255)

                                                                                    ggCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGttt                                                                   <  1:589142/150‑1 (MQ=255)

                                                                                     gcagcaCAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTg                                                                  <  2:1167939/150‑1 (MQ=255)

                                                                                       agcacagcaGACTGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTgca                                                                >  1:1504664/1‑150 (MQ=255)

                                                                                       agcacagcaGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGACTCACTACGTTTgca                                                                >  1:4465062/1‑150 (MQ=255)

                                                                                        gcacagcaGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTgcag                                                               <  1:3024888/150‑1 (MQ=255)

                                                                                         cacagcaGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTgcagc                                                              >  1:4622860/1‑150 (MQ=255)

                                                                                          acagcaGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTgcagca                                                             >  2:4730489/1‑150 (MQ=255)

                                                                                           cagcagAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGcagcag                                                            >  2:1443429/1‑150 (MQ=255)

                                                                                           cagcagAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGcagcag                                                            >  2:3068813/1‑150 (MQ=255)

                                                                                           cagcagAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGcagcag                                                            >  2:3546039/1‑150 (MQ=255)

                                                                                            agcagAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAgg                                                           >  1:891192/1‑150 (MQ=255)

                                                                                            agcagAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAgg                                                           >  1:4474193/1‑150 (MQ=255)

                                                                                              cagAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGcc                                                         >  1:2748970/1‑150 (MQ=255)

                                                                                               agAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCt                                                        >  1:3135051/1‑150 (MQ=255)

                                                                                                gaatgaatTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGGCCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCtt                                                       >  1:3235339/1‑150 (MQ=255)

                                                                                                gaatgaatTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCtt                                                       <  1:2356654/150‑1 (MQ=255)

                                                                                                      atTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGACCAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCga                                                 >  1:1240535/1‑150 (MQ=255)

                                                                                                       ttCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCgag                                                >  1:4680445/1‑150 (MQ=255)

                                                                                                        tCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCgaga                                               <  1:2126139/150‑1 (MQ=255)

                                                                                                            tAACATTGTCGCGCCACTGGCAGTGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacggg                                           <  1:3646505/150‑1 (MQ=255)

                                                                                                            tAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacggg                                           <  2:2244377/150‑1 (MQ=255)

                                                                                                             aaCATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacggga                                          >  1:4774633/1‑150 (MQ=255)

                                                                                                               cATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacgggacg                                        <  2:3870487/150‑1 (MQ=255)

                                                                                                               cATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacgggacg                                        >  2:3993377/1‑150 (MQ=255)

                                                                                                               cATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacgggacg                                        >  2:208798/1‑150 (MQ=255)

                                                                                                                aTTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacgggacgg                                       >  1:4205528/1‑150 (MQ=255)

                                                                                                                 ttGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGacgggacggg                                      >  1:4438293/1‑150 (MQ=255)

                                                                                                                   gTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGAt                                    <  2:2933908/150‑1 (MQ=255)

                                                                                                                    tCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATa                                   <  1:3278314/150‑1 (MQ=255)

                                                                                                                       cgcCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAAc                                >  1:2223247/1‑150 (MQ=255)

                                                                                                                       cgcCACGGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTGAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAAc                                <  2:2850492/150‑1 (MQ=255)

                                                                                                                        gcCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACt                               <  1:241256/150‑1 (MQ=255)

                                                                                                                        gcCACTGGCAGAGTGGGAAGCGACAAACCTCCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACt                               >  1:2864617/1‑150 (MQ=255)

                                                                                                                         ccACGGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTg                              <  2:2413628/150‑1 (MQ=255)

                                                                                                                                   agTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGtt                    <  2:4254478/150‑1 (MQ=255)

                                                                                                                                       ggAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATg                >  1:708510/1‑150 (MQ=255)

                                                                                                                                              aCAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCa         >  2:2284596/1‑150 (MQ=255)

                                                                                                                                               cAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGACAg        <  1:4401322/150‑1 (MQ=255)

                                                                                                                                                  accaccTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGgcg     >  2:2391036/1‑150 (MQ=255)

                                                                                                                                                     accTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGgcgtgc  <  1:1013556/150‑1 (MQ=255)

GGTGAGATGTACCGCCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGC  >  CP009273/4407158‑4407456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`