BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,428,092	G→T	51.7%	G190V (GGA→GTA)	ytfI →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,428,092	0	G	T	51.7%	43.3 / inf	205	G190V (GGA→GTA)	ytfI	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (48/51); new base T (58/48); total (106/99)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

Only 100 of 215 total aligned reads displayed.

GGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAG  >  CP009273/4427943‑4428240

      tCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTgg                                                                                                                                                <  1:571495/150‑1 (MQ=255)

        tACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGaa                                                                                                                                              <  1:2182954/150‑1 (MQ=255)

         aCAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAAt                                                                                                                                             >  2:780166/1‑150 (MQ=255)

          cAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAAtt                                                                                                                                            <  1:2905243/150‑1 (MQ=255)

            gAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACTTGGCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAATCGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATaaa                                                                                                                                          >  1:3230599/1‑150 (MQ=255)

            gAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTaa                                                                                                                                          >  1:2481539/1‑150 (MQ=255)

                 aaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAAt                                                                                                                                     >  2:3220879/1‑150 (MQ=255)

                  aCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATg                                                                                                                                    >  1:621656/1‑150 (MQ=255)

                    aGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCg                                                                                                                                  <  1:4627726/150‑1 (MQ=255)

                       cTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatg                                                                                                                               <  1:954423/150‑1 (MQ=255)

                       cTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgatg                                                                                                                               <  2:888147/150‑1 (MQ=255)

                       cTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgatg                                                                                                                               <  2:3891640/150‑1 (MQ=255)

                        tGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgatgg                                                                                                                              <  1:1923477/150‑1 (MQ=255)

                        tGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgatgg                                                                                                                              >  2:1490032/1‑150 (MQ=255)

                         gACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgatgga                                                                                                                             >  1:3848869/1‑150 (MQ=255)

                          aCATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatggat                                                                                                                            <  1:4029565/150‑1 (MQ=255)

                          aCATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgatggat                                                                                                                            <  1:3271991/150‑1 (MQ=255)

                             tACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGCGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGt                                                                                                                         <  1:1500350/150‑1 (MQ=255)

                             tACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGt                                                                                                                         <  2:4200542/150‑1 (MQ=255)

                               ccGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGtat                                                                                                                       <  2:1708478/150‑1 (MQ=255)

                                  aaaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATAtt                                                                                                                    <  1:2443831/150‑1 (MQ=255)

                                  aaaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATAtt                                                                                                                    >  2:3010186/1‑150 (MQ=255)

                                  aaaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATAtt                                                                                                                    >  1:2009339/1‑150 (MQ=255)

                                  aaaaGGATTAAAGGTTATATTCACAAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGTGGTAAATTTGAACTCCATATACCAGGAGGTGGAATTAAAGAATGCGATGTATGGTATAtt                                                                                                                    >  2:1968870/1‑150 (MQ=255)

                                   aaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATAttt                                                                                                                   >  2:598400/1‑150 (MQ=255)

                                     aGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTAc                                                                                                                 <  1:2286546/150‑1 (MQ=255)

                                       gATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGa                                                                                                               >  2:1241304/1‑150 (MQ=255)

                                        aTTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaa                                                                                                              >  1:3570254/1‑150 (MQ=255)

                                        aTTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaa                                                                                                              <  2:3230154/150‑1 (MQ=255)

                                         ttAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaaa                                                                                                             >  1:3285869/1‑150 (MQ=255)

                                              ggTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaata                                                                                                        <  1:3079092/150‑1 (MQ=255)

                                              ggTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaata                                                                                                        <  1:3430079/150‑1 (MQ=255)

                                              ggTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaata                                                                                                        <  1:2425576/150‑1 (MQ=255)

                                                 tataTTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGACTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAAtt                                                                                                     <  1:4374497/150‑1 (MQ=255)

                                                   tatTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtga                                                                                                   <  2:4192931/150‑1 (MQ=255)

                                                    atTCACCAGGCGATTGTTGTCGTGTCTTTAGGTTATTCCTTATTGGAGGATATAGTAAGCAAATTAAAACTGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtgat                                                                                                  <  2:4244196/150‑1 (MQ=255)

                                                    atTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtgat                                                                                                  <  1:4441620/150‑1 (MQ=255)

                                                    atTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtgat                                                                                                  <  1:2981348/150‑1 (MQ=255)

                                                       cACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTgatgat                                                                                               <  1:1192296/150‑1 (MQ=255)

                                                       cACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTgatgat                                                                                               >  1:828621/1‑150 (MQ=255)

                                                        aCCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATa                                                                                              <  1:1063056/150‑1 (MQ=255)

                                                          cAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAt                                                                                            <  2:4348157/150‑1 (MQ=255)

                                                           aGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAtt                                                                                           >  1:3658391/1‑150 (MQ=255)

                                                            ggCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAttt                                                                                          <  1:3271932/150‑1 (MQ=255)

                                                            ggCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAttt                                                                                          <  2:4619936/150‑1 (MQ=255)

                                                               gATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGc                                                                                       >  2:709178/1‑150 (MQ=255)

                                                               gATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTga                                                                                       >  1:423507/1‑149 (MQ=255)

                                                                aTTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGcc                                                                                      >  1:3870179/1‑150 (MQ=255)

                                                                   ggTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATATTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATa                                                                                   <  2:2834733/150‑1 (MQ=255)

                                                                    gTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAg                                                                                  <  1:3495280/150‑1 (MQ=255)

                                                                      gACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTc                                                                                >  1:3356810/1‑150 (MQ=255)

                                                                        cGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCAt                                                                              <  1:370031/150‑1 (MQ=255)

                                                                           gtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGa                                                                           >  1:1242645/1‑150 (MQ=255)

                                                                           gtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGa                                                                           >  1:1242581/1‑150 (MQ=255)

                                                                                 aGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCttt                                                                     <  2:3898111/150‑1 (MQ=255)

                                                                                 aGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCttt                                                                     <  2:1153664/150‑1 (MQ=255)

                                                                                   gTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTgg                                                                   >  2:3584219/1‑150 (MQ=255)

                                                                                        gCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCttt                                                              <  1:2117200/150‑1 (MQ=255)

                                                                                           ttATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgcg                                                           >  2:265978/1‑150 (MQ=255)

                                                                                           ttATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgcg                                                           >  2:266469/1‑150 (MQ=255)

                                                                                             aTTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCt                                                         >  1:480362/1‑150 (MQ=255)

                                                                                              ttGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCtt                                                        >  1:2770139/1‑150 (MQ=255)

                                                                                              ttGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCtt                                                        >  1:505843/1‑150 (MQ=255)

                                                                                              ttGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCtt                                                        >  2:1068737/1‑150 (MQ=255)

                                                                                               tGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTa                                                       >  2:2151941/1‑150 (MQ=255)

                                                                                               tGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTa                                                       >  1:4312758/1‑150 (MQ=255)

                                                                                                 gaggaTATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTaaa                                                     >  2:3792258/1‑150 (MQ=255)

                                                                                                  aggaTATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTaaaa                                                    >  1:1134094/1‑150 (MQ=255)

                                                                                                  aggaTATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTaaaa                                                    >  2:260250/1‑150 (MQ=255)

                                                                                                       ataGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTAt                                               >  1:1894569/1‑150 (MQ=255)

                                                                                                         aGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATgg                                             >  2:486031/1‑150 (MQ=255)

                                                                                                           tAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAg                                           >  1:1095379/1‑150 (MQ=255)

                                                                                                            aaGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAgg                                          <  2:3496495/150‑1 (MQ=255)

                                                                                                            aaGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAgg                                          >  2:3532045/1‑150 (MQ=255)

                                                                                                            aaGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAgg                                          <  2:1454530/150‑1 (MQ=255)

                                                                                                                aaaTTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGAt                                      <  2:4715988/150‑1 (MQ=255)

                                                                                                                 aaTTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATg                                     >  2:2908292/1‑150 (MQ=255)

                                                                                                                   ttAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGaa                                   <  2:2209504/150‑1 (MQ=255)

                                                                                                                    tAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAAc                                  <  2:1770282/150‑1 (MQ=255)

                                                                                                                         cGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGtttt                             <  1:3220879/150‑1 (MQ=255)

                                                                                                                          ggggTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTc                            <  2:3887109/150‑1 (MQ=255)

                                                                                                                          ggggTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTc                            <  2:232925/150‑1 (MQ=255)

                                                                                                                            ggTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGt                          <  2:1347472/150‑1 (MQ=255)

                                                                                                                              tAAATTTGAACTCCAAATACCCGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTaa                        >  2:4529280/1‑150 (MQ=255)

                                                                                                                              tAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTaa                        <  1:780166/150‑1 (MQ=255)

                                                                                                                                 aTTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATgg                     <  2:4124763/150‑1 (MQ=255)

                                                                                                                                 aTTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATgg                     <  2:2877830/150‑1 (MQ=255)

                                                                                                                                     gAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTcc                 >  1:2876089/1‑150 (MQ=255)

                                                                                                                                      aaCTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCct                >  2:544504/1‑150 (MQ=255)

                                                                                                                                        cTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCctct              >  2:2235761/1‑150 (MQ=255)

                                                                                                                                         tCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtg             <  2:3841342/150‑1 (MQ=255)

                                                                                                                                             aaTACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgttgt         >  2:2077188/1‑150 (MQ=255)

                                                                                                                                             aaTACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgttgt         >  1:798010/1‑150 (MQ=255)

                                                                                                                                               tACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGttt       >  2:3928576/1‑150 (MQ=255)

                                                                                                                                                aCCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGtttt      <  2:3870179/150‑1 (MQ=255)

                                                                                                                                                   aggaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTgga   <  2:3285822/150‑1 (MQ=255)

                                                                                                                                                   aggaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTgga   >  2:1341807/1‑150 (MQ=255)

                                                                                                                                                   aggaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTgga   >  2:3753544/1‑150 (MQ=255)

                                                                                                                                                   aggaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTgga   >  2:3093098/1‑150 (MQ=255)

                                                                                                                                                    ggaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTggag  <  2:1557492/150‑1 (MQ=255)

GGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGAG  >  CP009273/4427943‑4428240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`