BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,465,836	C→T	18.8%	L195L (CTA→TTA)	yjgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,465,836	0	C	T	18.8%	350.9 / 68.0	138	L195L (CTA→TTA)	yjgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (46/66); new base T (15/11); total (61/77)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 138 total aligned reads displayed.

TTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACA  >  CP009273/4465688‑4465984

ttCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCt                                                                                                                                                     <  2:3855804/150‑1 (MQ=255)

 tCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTa                                                                                                                                                    <  1:283285/150‑1 (MQ=255)

     cTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGa                                                                                                                                                <  2:3361335/150‑1 (MQ=255)

             tATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAaat                                                                                                                                        <  1:169130/150‑1 (MQ=255)

               tGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAaataa                                                                                                                                      <  1:599812/150‑1 (MQ=255)

               tGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAaataa                                                                                                                                      <  1:2305833/150‑1 (MQ=255)

                   gggAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTa                                                                                                                                  >  1:2236365/1‑150 (MQ=255)

                     gAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAAGAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACa                                                                                                                                <  2:659344/150‑1 (MQ=255)

                       aGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGa                                                                                                                              >  2:1321815/1‑150 (MQ=255)

                           cTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGtt                                                                                                                          >  2:126035/1‑150 (MQ=255)

                           cTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGtt                                                                                                                          >  2:356894/1‑150 (MQ=255)

                            tttGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTa                                                                                                                         <  1:4503912/150‑1 (MQ=255)

                            tttGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTa                                                                                                                         <  2:3833995/150‑1 (MQ=255)

                            tttGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTa                                                                                                                         <  2:1424152/150‑1 (MQ=255)

                              tGAAAGAAAAGATGAGAGTTCATTACACCTTATTACTAACTAGGTTCTGGCGTGTTAACAAATTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTTAATAATCTACAGAAATTAAATAATCTACAGAAGTTaaa                                                                                                                       <  2:1787957/150‑1 (MQ=255)

                              tGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTaaa                                                                                                                       >  2:2773032/1‑150 (MQ=255)

                              tGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATATACAGAAGTTaaa                                                                                                                       >  1:2710574/1‑150 (MQ=255)

                               gaaagaaaAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaat                                                                                                                      <  1:357615/150‑1 (MQ=255)

                                 aagaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaataa                                                                                                                    >  1:1795519/1‑150 (MQ=255)

                                 aagaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaataa                                                                                                                    >  1:1792836/1‑150 (MQ=255)

                                 aagaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaataa                                                                                                                    >  1:3685185/1‑150 (MQ=255)

                                 aagaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaataa                                                                                                                    >  1:3685436/1‑150 (MQ=255)

                                 aagaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAaataa                                                                                                                    >  1:3918475/1‑150 (MQ=255)

                                   gaaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATc                                                                                                                  <  1:1432814/150‑1 (MQ=255)

                                    aaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCt                                                                                                                 >  1:126607/1‑150 (MQ=255)

                                    aaaagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCt                                                                                                                 >  1:126054/1‑150 (MQ=255)

                                      aagaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAACTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTAc                                                                                                               <  1:3543946/150‑1 (MQ=255)

                                        gaTGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTg                                                                                                             <  2:1906860/150‑1 (MQ=255)

                                             gagTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGtt                                                                                                        <  1:1020963/150‑1 (MQ=255)

                                              agTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTa                                                                                                       <  2:1853786/150‑1 (MQ=255)

                                               gTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTaa                                                                                                      <  1:565869/150‑1 (MQ=255)

                                               gTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTaa                                                                                                      >  2:2637694/1‑150 (MQ=255)

                                                ttCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTaaa                                                                                                     <  2:1184180/150‑1 (MQ=255)

                                                ttCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTaaa                                                                                                     <  1:4550951/150‑1 (MQ=255)

                                                 tCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaat                                                                                                    <  1:3308048/150‑1 (MQ=255)

                                                  cATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaata                                                                                                   >  2:347891/1‑150 (MQ=255)

                                                   aTTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATCATCAGGAGTTAAATAATCTATAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaataa                                                                                                  >  2:640944/1‑150 (MQ=255)

                                                   aTTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATCATCAGGAGTTAAATAATCTATAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaataa                                                                                                  >  2:2073788/1‑150 (MQ=255)

                                                   aTTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATCATCAGGAGTTAAATAATCTATAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaataa                                                                                                  >  2:640949/1‑150 (MQ=255)

                                                   aTTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAaataa                                                                                                  <  2:4608546/150‑1 (MQ=255)

                                                      acacCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAatat                                                                                               <  2:3481156/150‑1 (MQ=255)

                                                         ccTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACa                                                                                            <  1:4606512/150‑1 (MQ=255)

                                                                   aaCAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAaat                                                                                  >  2:4491798/1‑150 (MQ=255)

                                                                    aCAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAaata                                                                                 <  1:122258/150‑1 (MQ=255)

                                                                     cAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAaataa                                                                                <  2:496575/150‑1 (MQ=255)

                                                                     cAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAaataa                                                                                >  2:3072878/1‑150 (MQ=255)

                                                                                    ttACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTaaa                                                                 <  1:64320/150‑1 (MQ=255)

                                                                                      aCCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaata                                                               >  1:881571/1‑150 (MQ=255)

                                                                                      aCCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaata                                                               <  2:1007998/150‑1 (MQ=255)

                                                                                      aCCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaata                                                               >  2:3205356/1‑150 (MQ=255)

                                                                                       ccAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaataa                                                              <  2:1053640/150‑1 (MQ=255)

                                                                                         aaaGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATc                                                            >  1:1594808/1‑150 (MQ=255)

                                                                                            gTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGc                                                         >  2:2904242/1‑150 (MQ=255)

                                                                                            gTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGc                                                         <  1:840542/150‑1 (MQ=255)

                                                                                               aaGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGa                                                      <  1:2958567/150‑1 (MQ=255)

                                                                                               aaGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGa                                                      <  1:2958520/150‑1 (MQ=255)

                                                                                                   tAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                                  >  1:1786062/1‑150 (MQ=255)

                                                                                                    aaCAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTa                                                 <  2:3127538/150‑1 (MQ=255)

                                                                                                    aaCAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTa                                                 >  2:3887192/1‑150 (MQ=255)

                                                                                                     aCAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTaa                                                >  1:106467/1‑150 (MQ=255)

                                                                                                              tAGCGCGTCTATTAAATTATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATtctc                                       <  1:945201/150‑1 (MQ=255)

                                                                                                              tAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATtctc                                       >  1:3921393/1‑150 (MQ=255)

                                                                                                              tAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATtctc                                       <  2:555446/150‑1 (MQ=255)

                                                                                                               aGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCa                                      <  2:4108293/150‑1 (MQ=255)

                                                                                                                 cgcgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAgg                                    <  1:1251224/150‑1 (MQ=255)

                                                                                                                  gcgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGa                                   <  2:2993680/150‑1 (MQ=255)

                                                                                                                  gcgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGa                                   >  1:1588468/1‑150 (MQ=255)

                                                                                                                    gTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGt                                 >  2:2323054/1‑150 (MQ=255)

                                                                                                                     tCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGtt                                >  2:2677577/1‑150 (MQ=255)

                                                                                                                      cTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTa                               <  2:3110860/150‑1 (MQ=255)

                                                                                                                        aTTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa                             <  2:3685185/150‑1 (MQ=255)

                                                                                                                        aTTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa                             >  1:2059641/1‑150 (MQ=255)

                                                                                                                        aTTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa                             <  2:1795519/150‑1 (MQ=255)

                                                                                                                        aTTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa                             <  2:1792836/150‑1 (MQ=255)

                                                                                                                          tAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATa                           >  2:2145400/1‑150 (MQ=255)

                                                                                                                           aaaTAATAATCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATaa                          <  1:3678045/150‑1 (MQ=255)

                                                                                                                               aataatCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCg                      >  1:657068/1‑150 (MQ=255)

                                                                                                                               aataatCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCg                      >  2:1527089/1‑150 (MQ=255)

                                                                                                                               aataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCg                      <  2:3918475/150‑1 (MQ=255)

                                                                                                                               aataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCg                      >  2:94154/1‑150 (MQ=255)

                                                                                                                                ataatCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATTATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGc                     >  1:3794805/1‑150 (MQ=255)

                                                                                                                                ataatCAGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGc                     >  1:3794822/1‑150 (MQ=255)

                                                                                                                                 taatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCa                    >  1:24776/1‑150 (MQ=255)

                                                                                                                                 taatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCa                    >  1:4117946/1‑150 (MQ=255)

                                                                                                                                  actCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAg                   <  1:1647404/148‑1 (MQ=255)

                                                                                                                                   atCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAgg                  <  1:1451835/150‑1 (MQ=255)

                                                                                                                                   atCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAgg                  <  1:1451813/150‑1 (MQ=255)

                                                                                                                                    tCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGa                 >  2:754546/1‑150 (MQ=255)

                                                                                                                                      aGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAt               >  1:707226/1‑150 (MQ=255)

                                                                                                                                      aGGAGTTAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAt               >  1:1366512/1‑150 (MQ=255)

                                                                                                                                        gAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTa             <  2:3531691/150‑1 (MQ=255)

                                                                                                                                           ttAAATAATTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAAt          >  2:4491946/1‑150 (MQ=255)

                                                                                                                                              aataatTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGt       >  1:2543061/1‑150 (MQ=255)

                                                                                                                                              aataatCGACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGt       <  1:2844628/150‑1 (MQ=255)

                                                                                                                                               ataatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTc      <  1:1072990/150‑1 (MQ=255)

                                                                                                                                                taatTTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTcc     <  2:1512788/150‑1 (MQ=255)

                                                                                                                                                 aatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCca    <  2:126607/150‑1 (MQ=255)

                                                                                                                                                 aatCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCca    <  2:126054/150‑1 (MQ=255)

                                                                                                                                                   tCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCcaca  >  1:1754878/1‑150 (MQ=255)

                                                                                                                                                   tCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCcaca  <  2:3482166/150‑1 (MQ=255)

TTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACA  >  CP009273/4465688‑4465984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`