BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,470,873	G→T	43.4%	R928S (CGT→AGT)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,470,873	0	G	T	43.4%	240.1 / inf	289	R928S (CGT→AGT)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (82/80); new base T (61/63); total (146/143)
Fisher's exact test for biased strand distribution p-value = 9.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

TTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGCCATCG  >  CP009273/4470728‑4471022

 ttACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTACCGCATAGCCTTACAGCTTCTCACTCTCtt                                                                                                                                                  >  1:3803786/1‑150 (MQ=255)

 ttACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTAAAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTACGCATAGCCTTCCAGCTTCTCACTCGCtt                                                                                                                                                  >  1:4574392/1‑150 (MQ=255)

  tACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCttt                                                                                                                                                 <  2:3885582/150‑1 (MQ=255)

   aCCTTTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTc                                                                                                                                                <  1:1022610/150‑1 (MQ=255)

   aCCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCGTTCGCGTCCGCATAGCCTTACAGCTTCTCACTCTCTTTc                                                                                                                                                >  1:885985/1‑150 (MQ=255)

    ccATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTcg                                                                                                                                               >  2:257981/1‑150 (MQ=255)

           tGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGAc                                                                                                                                        >  2:4341604/1‑150 (MQ=255)

            gTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGAcc                                                                                                                                       >  2:2313360/1‑150 (MQ=255)

            gTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGAcc                                                                                                                                       >  2:3254245/1‑150 (MQ=255)

             tAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCg                                                                                                                                      >  2:491884/1‑150 (MQ=255)

             tAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCg                                                                                                                                      >  2:3877666/1‑150 (MQ=255)

              aaGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGc                                                                                                                                     >  2:4387586/1‑150 (MQ=255)

              aaGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCGTCCAGCTTCTCACGCTCTTTCGCGATGACCGc                                                                                                                                     >  1:4436439/1‑150 (MQ=255)

               agagATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCt                                                                                                                                    <  1:869653/150‑1 (MQ=255)

                tagaTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCtt                                                                                                                                   >  2:3299678/2‑150 (MQ=255)

                 agaTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTc                                                                                                                                  <  1:1857636/150‑1 (MQ=255)

                   aTAAAAATGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCg                                                                                                                                >  2:2685029/1‑150 (MQ=255)

                   aTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCg                                                                                                                                <  2:2449796/150‑1 (MQ=255)

                     aaaaaaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGt                                                                                                                              >  2:2461989/1‑150 (MQ=255)

                     aaaaaaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGt                                                                                                                              >  2:898590/1‑150 (MQ=255)

                     aaaaaaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGt                                                                                                                              <  1:1366623/150‑1 (MQ=255)

                     aaaaaaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCCCATAGCCTGCCCGCAAAACACGCACATTCCCGATGACCGCTTCCGGt                                                                                                                              >  1:1199623/1‑150 (MQ=255)

                     aaaaaaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATAACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATAAGTTTAGCTTTCGCTTCCGCAAAGCCTGCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGt                                                                                                                              >  1:4099544/1‑150 (MQ=255)

                      aaaaaGGCCGGAGCATGCTCCGGCCATCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTg                                                                                                                             <  2:1655463/150‑1 (MQ=255)

                          aGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGcgcg                                                                                                                         <  2:4168719/150‑1 (MQ=255)

                            gCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCggg                                                                                                                       >  2:2553336/1‑150 (MQ=255)

                                    aTGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAg                                                                                                               >  2:2034508/1‑150 (MQ=255)

                                        tCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCtt                                                                                                           <  1:2729242/150‑1 (MQ=255)

                                         ccGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttc                                                                                                          <  1:4338710/150‑1 (MQ=255)

                                         ccGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttc                                                                                                          >  2:3216794/1‑150 (MQ=255)

                                         ccGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCATCCAGCATCACACGCTCTTTAGCGATTACCGCTTCCGGTGCGCGGGCGACAAAGccata                                                                                                          >  1:2011063/1‑147 (MQ=255)

                                           ggCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgt                                                                                                        >  2:4703563/1‑150 (MQ=255)

                                           ggCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgt                                                                                                        <  2:156536/150‑1 (MQ=255)

                                                 cGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCa                                                                                                  >  2:3544924/1‑150 (MQ=255)

                                                   ttttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGt                                                                                                <  1:339615/150‑1 (MQ=255)

                                                   ttttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGt                                                                                                >  1:3333028/1‑150 (MQ=255)

                                                     ttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGttt                                                                                              <  2:2884547/150‑1 (MQ=255)

                                                     ttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCGTCTCACTCTCTTTCGCGATGACCGCTTCCGGGGCGCGGGCGACAAAGCCTTCGTTCGCCAGttt                                                                                              <  1:2569612/150‑1 (MQ=255)

                                                       catcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGt                                                                                            >  2:641354/1‑150 (MQ=255)

                                                        atcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGtt                                                                                           <  2:1408119/150‑1 (MQ=255)

                                                          caCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtct                                                                                         <  1:4253299/150‑1 (MQ=255)

                                                          caCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtct                                                                                         >  1:3543145/1‑150 (MQ=255)

                                                           aCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtctc                                                                                        <  2:3458765/150‑1 (MQ=255)

                                                           aCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtctc                                                                                        <  1:2079740/150‑1 (MQ=255)

                                                            cTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCg                                                                                       >  1:3770078/1‑150 (MQ=255)

                                                               tgtTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATa                                                                                    <  2:1797871/150‑1 (MQ=255)

                                                                     gATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTg                                                                              >  1:644672/1‑150 (MQ=255)

                                                                     gATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTg                                                                              <  2:2464711/150‑1 (MQ=255)

                                                                           aGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCa                                                                        >  2:3334709/1‑150 (MQ=255)

                                                                                     tAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCt                                                              <  1:3730323/150‑1 (MQ=255)

                                                                                     tAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCt                                                              <  2:2902149/150‑1 (MQ=255)

                                                                                      aaCAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCtt                                                             >  2:940251/1‑150 (MQ=255)

                                                                                      aaCAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCtt                                                             >  2:752269/1‑150 (MQ=255)

                                                                                      aaCAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCtt                                                             <  2:4586544/150‑1 (MQ=255)

                                                                                       aCAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTc                                                            <  2:1881403/150‑1 (MQ=255)

                                                                                        cAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCg                                                           >  1:2434481/1‑150 (MQ=255)

                                                                                          gCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGcc                                                         >  1:2920890/1‑150 (MQ=255)

                                                                                          gCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGcc                                                         <  2:3986034/150‑1 (MQ=255)

                                                                                           ccTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCa                                                        >  1:4765848/1‑150 (MQ=255)

                                                                                            ctgctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCAc                                                       >  2:3317202/1‑150 (MQ=255)

                                                                                              gctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATTACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActt                                                     >  2:405933/1‑150 (MQ=255)

                                                                                              gctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActt                                                     <  2:4767334/150‑1 (MQ=255)

                                                                                               ctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActtc                                                    >  2:2389528/1‑150 (MQ=255)

                                                                                               ctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActtc                                                    >  2:1364304/1‑150 (MQ=255)

                                                                                                  ttCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCttt                                                 >  2:2983572/1‑150 (MQ=255)

                                                                                                     aaTCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGc                                              >  1:1179481/1‑150 (MQ=255)

                                                                                                       tCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCa                                            >  2:1759787/1‑150 (MQ=255)

                                                                                                        cAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAg                                           <  1:258206/150‑1 (MQ=255)

                                                                                                         aGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCCCTTCTTTCGCCAGa                                          >  2:2602811/1‑150 (MQ=255)

                                                                                                               gctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGcc                                    <  2:4481033/150‑1 (MQ=255)

                                                                                                               gctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGcc                                    >  2:366341/1‑150 (MQ=255)

                                                                                                                ctttcgcttCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCa                                   <  1:4786894/150‑1 (MQ=255)

                                                                                                                ctttcgcttCCGCATAGCCTTCCAGCGTCTCACTCTCTTTCGCGAGGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCa                                   <  2:4040748/150‑1 (MQ=255)

                                                                                                                 tttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAg                                  >  2:4460587/1‑150 (MQ=255)

                                                                                                                 tttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAg                                  >  2:1078021/1‑150 (MQ=255)

                                                                                                                 tttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAg                                  >  1:536978/1‑150 (MQ=255)

                                                                                                                       ttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                            <  2:2723229/150‑1 (MQ=255)

                                                                                                                          cGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtcttc                         >  1:2231178/1‑150 (MQ=255)

                                                                                                                             aTAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCttt                      >  2:309712/1‑150 (MQ=255)

                                                                                                                             aTAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCttt                      <  1:3132399/150‑1 (MQ=255)

                                                                                                                              tAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttg                     >  1:1274108/1‑150 (MQ=255)

                                                                                                                              tAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttg                     <  1:3254241/150‑1 (MQ=255)

                                                                                                                                gCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgtt                   <  1:3590204/150‑1 (MQ=255)

                                                                                                                                gCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgtt                   <  2:3769599/150‑1 (MQ=255)

                                                                                                                                 ccTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgttg                  <  2:2135312/150‑1 (MQ=255)

                                                                                                                                 ccTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgttg                  >  1:2273154/1‑150 (MQ=255)

                                                                                                                                  cTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtga                 <  2:2860130/150‑1 (MQ=255)

                                                                                                                                  cTTCAAGCTTCTCACTCTCTTTCGCGATGACCGCTTCAGGTGCGCTGGCGACAAATCCATCGTTCGAAAGTTAGTAATCGATACGGCTGATTTCACATTCAATCTTAGCCACTTCTATCGACAGACGCGCCAGATCATCTTCTATGTtga                 >  2:539761/1‑150 (MQ=255)

                                                                                                                                   ttCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTGTGTtgat                >  1:1597024/1‑150 (MQ=255)

                                                                                                                                    tCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatg               >  1:3051659/1‑150 (MQ=255)

                                                                                                                                     ccAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatga              <  2:3803786/150‑1 (MQ=255)

                                                                                                                                      cAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAg             <  1:2493331/150‑1 (MQ=255)

                                                                                                                                       aGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAgg            >  1:1690137/1‑150 (MQ=255)

                                                                                                                                       aGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAgg            <  2:1152802/150‑1 (MQ=255)

                                                                                                                                       aGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAgg            >  1:805835/1‑150 (MQ=255)

                                                                                                                                         cTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgcc          <  1:4387549/150‑1 (MQ=255)

                                                                                                                                            ctcACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccagc       >  1:3926108/1‑150 (MQ=255)

                                                                                                                                             tcACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccagcc      <  1:3027505/150‑1 (MQ=255)

                                                                                                                                                 gCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGCCATCg  >  1:3617099/1‑150 (MQ=255)

                                                                                                                                                 gCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGCCATCg  >  2:804502/1‑150 (MQ=255)

TTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGCCATCG  >  CP009273/4470728‑4471022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`