Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,473,750 G→T 9.5% F116L (TTC→TTA)  holC ← DNA polymerase III, chi subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,473,7500GT 9.5% 1269.1 / 73.6 353F116L (TTC→TTA) holCDNA polymerase III, chi subunit
Reads supporting (aligned to +/- strand):  ref base G (165/154);  new base T (22/12);  total (187/166)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 364 total aligned reads displayed.

CCAGTGCTCGTAAAGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTAAATTATG  >  CP009273/4473601‑4473899
                                                                                                                                                     |                                                                                                                                                     
ccAGTGCTCGTAAAGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACg                                                                                                                                                       <  2:4059011/150‑1 (MQ=255)
    tGCTCGTAAAGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGt                                                                                                                                                   <  2:2718901/150‑1 (MQ=255)
      cTCGTAAAGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCt                                                                                                                                                 <  2:696212/150‑1 (MQ=255)
         gTAAAGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTAcc                                                                                                                                              <  1:3532840/150‑1 (MQ=255)
           aaaGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCAc                                                                                                                                            >  1:2314088/1‑150 (MQ=255)
            aaGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACt                                                                                                                                           >  2:1609644/1‑150 (MQ=255)
            aaGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACt                                                                                                                                           >  1:2855491/1‑150 (MQ=255)
                ggCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCtg                                                                                                                                       <  1:2218444/150‑1 (MQ=255)
                      tCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAg                                                                                                                                 >  2:87388/1‑150 (MQ=255)
                      tCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAg                                                                                                                                 >  2:1113486/1‑150 (MQ=255)
                       cGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGc                                                                                                                                <  1:3119066/150‑1 (MQ=255)
                         atatCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCgg                                                                                                                              >  2:622789/1‑150 (MQ=255)
                           atCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTg                                                                                                                            >  1:753401/1‑150 (MQ=255)
                              ttGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCa                                                                                                                         <  1:1785739/150‑1 (MQ=255)
                                gtgGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGATTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCaaa                                                                                                                       >  1:372345/1‑150 (MQ=255)
                                  gggTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAAt                                                                                                                     >  2:3380049/1‑150 (MQ=255)
                                   ggTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATc                                                                                                                    >  1:1709260/1‑150 (MQ=255)
                                    gTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCt                                                                                                                   <  1:2585904/150‑1 (MQ=255)
                                    gTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCt                                                                                                                   <  1:1859365/150‑1 (MQ=255)
                                     ttATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTg                                                                                                                  <  2:3734386/150‑1 (MQ=255)
                                     ttATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTg                                                                                                                  <  1:4694954/150‑1 (MQ=255)
                                     ttATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTg                                                                                                                  >  1:4302811/1‑150 (MQ=255)
                                     ttATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTg                                                                                                                  <  1:3838921/150‑1 (MQ=255)
                                       atatGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCa                                                                                                                >  1:4092287/1‑150 (MQ=255)
                                       atatGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCa                                                                                                                >  2:2914092/1‑150 (MQ=255)
                                        tatGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCaa                                                                                                               >  2:750441/1‑150 (MQ=255)
                                        tatGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCaa                                                                                                               >  2:3136691/1‑150 (MQ=255)
                                          tGTCTTTTCCATTATTTCCTGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAg                                                                                                             <  2:2514299/150‑1 (MQ=255)
                                           gTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGc                                                                                                            <  1:1096400/150‑1 (MQ=255)
                                             cTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCtt                                                                                                          <  2:3688668/150‑1 (MQ=255)
                                               tttCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGt                                                                                                        >  2:1997135/1‑150 (MQ=255)
                                                 tCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTc                                                                                                      <  2:4709985/150‑1 (MQ=255)
                                                  ccATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCg                                                                                                     <  2:2821769/150‑1 (MQ=255)
                                                    attattTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCa                                                                                                   >  1:615322/1‑150 (MQ=255)
                                                      tattTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGa                                                                                                 >  2:1932869/1‑150 (MQ=255)
                                                      tattTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGa                                                                                                 >  1:4519683/1‑150 (MQ=255)
                                                      tattTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGa                                                                                                 >  1:4391871/1‑150 (MQ=255)
                                                      tattTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGa                                                                                                 >  1:2721836/1‑150 (MQ=255)
                                                       attTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGAc                                                                                                >  1:2058901/1‑150 (MQ=255)
                                                       attTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGAc                                                                                                <  1:3386492/150‑1 (MQ=255)
                                                          tCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTaa                                                                                             <  2:4204999/150‑1 (MQ=255)
                                                           ccAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAAt                                                                                            >  2:3211505/1‑150 (MQ=255)
                                                            cAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATc                                                                                           <  1:992974/150‑1 (MQ=255)
                                                            cAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCACAGACTAATc                                                                                           <  2:1709260/150‑1 (MQ=255)
                                                             aGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCGACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCa                                                                                          <  2:3593315/150‑1 (MQ=255)
                                                              ggTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCaa                                                                                         <  1:1725720/150‑1 (MQ=255)
                                                                ttGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAata                                                                                       <  2:4259540/150‑1 (MQ=255)
                                                                  gCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAatata                                                                                     <  1:2226250/150‑1 (MQ=255)
                                                                   ccGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAatatat                                                                                    >  2:4478725/1‑150 (MQ=255)
                                                                    cGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATc                                                                                   >  2:2381181/1‑150 (MQ=255)
                                                                     gTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATcg                                                                                  <  2:2869101/150‑1 (MQ=255)
                                                                      tATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATCAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATcgc                                                                                 >  1:3632019/1‑150 (MQ=255)
                                                                      tATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATcgc                                                                                 >  1:3006077/1‑150 (MQ=255)
                                                                      tATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATcgc                                                                                 >  2:2327341/1‑150 (MQ=255)
                                                                       aTTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATcgcg                                                                                <  1:4265967/150‑1 (MQ=255)
                                                                         tCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGcc                                                                              <  2:2686359/150‑1 (MQ=255)
                                                                          cAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCg                                                                             >  2:3854003/1‑150 (MQ=255)
                                                                            ggTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGgc                                                                           >  1:3132032/1‑150 (MQ=255)
                                                                             gTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGgct                                                                          >  2:393942/1‑150 (MQ=255)
                                                                             gTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGgct                                                                          >  1:144586/1‑150 (MQ=255)
                                                                               tGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGgctgc                                                                        >  2:1301897/1‑150 (MQ=255)
                                                                                gAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGgctgct                                                                       <  2:4027345/150‑1 (MQ=255)
                                                                                    ccAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTa                                                                   <  2:1720070/150‑1 (MQ=255)
                                                                                      aGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACg                                                                 >  1:1681256/1‑150 (MQ=255)
                                                                                          aCGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCttt                                                             <  1:2493413/150‑1 (MQ=255)
                                                                                           cgcgGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCtttt                                                            <  2:3988438/150‑1 (MQ=255)
                                                                                           cgcgGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCtttt                                                            <  1:3873452/150‑1 (MQ=255)
                                                                                            gcgGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTg                                                           >  2:1341159/1‑150 (MQ=255)
                                                                                              ggTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTCATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCg                                                         >  1:2403935/1‑150 (MQ=255)
                                                                                              ggTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCg                                                         >  1:4150407/1‑150 (MQ=255)
                                                                                                  ggCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCa                                                     >  2:1548963/1‑150 (MQ=255)
                                                                                                    cTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAgg                                                   >  1:1913821/1‑150 (MQ=255)
                                                                                                      ttATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCg                                                 <  1:1246296/150‑1 (MQ=255)
                                                                                                       tataGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGa                                                >  2:1056195/1‑150 (MQ=255)
                                                                                                          aGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGAtct                                             >  1:1618038/1‑150 (MQ=255)
                                                                                                           gCGTTCGCGCGCCAGTTGTTTCAGAGTATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCTCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGAtctc                                            <  1:3642886/150‑1 (MQ=255)
                                                                                                            cGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATACGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTcc                                           <  2:4654409/150‑1 (MQ=255)
                                                                                                            cGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTcc                                           <  2:4541136/150‑1 (MQ=255)
                                                                                                             gTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCa                                          >  2:2984147/1‑150 (MQ=255)
                                                                                                                  cgcgcCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGt                                     <  2:4125805/150‑1 (MQ=255)
                                                                                                                     gcCAGTTGTTTCAGAGAATCTTCATAAGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCa                                  >  2:1779995/1‑150 (MQ=255)
                                                                                                                     gcCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGAGGCCAGGCGATCTCCACCGGTGCa                                  >  1:1012307/1‑150 (MQ=255)
                                                                                                                           tgttTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCAccgccg                            <  1:817900/150‑1 (MQ=255)
                                                                                                                            gttTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACcgccgc                           >  2:2697764/1‑150 (MQ=255)
                                                                                                                              ttCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCcgcgc                         <  2:4751339/150‑1 (MQ=255)
                                                                                                                                    gaATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCt                   <  1:194598/150‑1 (MQ=255)
                                                                                                                                      aTCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCTGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTtc                 >  1:1487994/1‑150 (MQ=255)
                                                                                                                                         ttCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTcc              <  1:1262222/150‑1 (MQ=255)
                                                                                                                                          tCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTccc             <  2:3463899/150‑1 (MQ=255)
                                                                                                                                          tCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTccc             <  2:1571562/150‑1 (MQ=255)
                                                                                                                                             tAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCt          >  2:1244420/1‑150 (MQ=255)
                                                                                                                                              aaGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTa         >  2:4420516/1‑150 (MQ=255)
                                                                                                                                               aGGAACTAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTaa        >  2:2163767/1‑150 (MQ=255)
                                                                                                                                                ggAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTaaa       >  1:3232492/1‑150 (MQ=255)
                                                                                                                                                 gAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTAAAt      >  1:1862316/1‑150 (MQ=255)
                                                                                                                                                 gAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTAAAt      >  2:2345977/1‑150 (MQ=255)
                                                                                                                                                  aaCGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTCAAtt     >  2:520059/1‑150 (MQ=255)
                                                                                                                                                    cGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTAAATTAt   <  2:4162816/150‑1 (MQ=255)
                                                                                                                                                     gAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTAAATTATg  <  1:498302/150‑1 (MQ=255)
                                                                                                                                                     gAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTAAATTATg  >  2:2304707/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGTGCTCGTAAAGCGGCTGTTCGATATCTTGTGGGTTATATGTCTTTTCCATTATTTCCAGGTTGCCGTATTCAGGTTGAAACCAGCCACGCGGTAGGCTTTATAGCGTTCGCGCGCCAGTTGTTTCAGAGAATCTTCATAAGGAACGAAGTCTACCACTTCTGTGAAAGCGGTGGCAAAATCTGCAAAGCTTGTTCGCAGACTAATCAATATATCGCGCCGGCTGCTGCTACGCTTTTGCGGCCAGGCGATCTCCACCGGTGCACCGCCGCGCGGTCCTTCTCCCGCTAAATTATG  >  CP009273/4473601‑4473899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑