BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,494,721	G→A	43.0%	A283T (GCC→ACC)	yjhB →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,494,721	0	G	A	43.0%	87.6 / 211.2	156	A283T (GCC→ACC)	yjhB	putative transporter
Reads supporting (aligned to +/- strand): ref base G (35/53); new base A (22/45); total (58/98)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

Only 100 of 158 total aligned reads displayed.

GGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATA  >  CP009273/4494572‑4494867

ggCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAg                                                                                                                                                    <  2:1493484/150‑1 (MQ=255)

   cGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCt                                                                                                                                                 <  2:4172380/150‑1 (MQ=255)

   cGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCt                                                                                                                                                 <  2:3168873/150‑1 (MQ=255)

      tAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTg                                                                                                                                              <  1:2313437/150‑1 (MQ=255)

      tAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTg                                                                                                                                              <  2:4472348/150‑1 (MQ=255)

      tAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTg                                                                                                                                              <  1:1875797/150‑1 (MQ=255)

       aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGt                                                                                                                                             <  2:1431787/150‑1 (MQ=255)

       aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGt                                                                                                                                             <  2:2897879/150‑1 (MQ=255)

           ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTc                                                                                                                                         <  1:3720897/150‑1 (MQ=255)

           ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTc                                                                                                                                         >  1:4737571/1‑150 (MQ=255)

             actactTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCgg                                                                                                                                       <  2:95073/150‑1 (MQ=255)

                 cttccttcCTACCTGGCAGATAATGGAGTTAATACAGTGGTAATTACAACTCTGATGACAATAGCAGGTATAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGTTGATAATATTGGTGT‑AAAAAAAGACTTTGTATTCGGTCta                                                                                                                                   >  2:4105451/1‑150 (MQ=255)

                  ttccttccTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCtaa                                                                                                                                  >  2:1606091/1‑150 (MQ=255)

                   tccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCtaat                                                                                                                                 <  2:822417/150‑1 (MQ=255)

                    ccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCtaata                                                                                                                                <  1:1285365/150‑1 (MQ=255)

                    ccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCtaata                                                                                                                                >  2:3327315/1‑150 (MQ=255)

                     cttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCtaataa                                                                                                                               <  2:3873636/150‑1 (MQ=255)

                     cttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCtaataa                                                                                                                               >  1:3506482/1‑150 (MQ=255)

                       tcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACt                                                                                                                             <  1:1701488/150‑1 (MQ=255)

                        cctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACtt                                                                                                                            <  2:3778972/150‑1 (MQ=255)

                        cctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACtt                                                                                                                            <  2:1328902/150‑1 (MQ=255)

                        cctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACtt                                                                                                                            <  2:1327405/150‑1 (MQ=255)

                         ctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTc                                                                                                                           <  1:3263724/150‑1 (MQ=255)

                             cTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTAGGTCTTATTACTTCattt                                                                                                                       >  1:2164751/1‑150 (MQ=255)

                              tGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTTGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCattt                                                                                                                       >  1:1641409/1‑150 (MQ=255)

                              tGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCattta                                                                                                                      >  1:4321683/1‑150 (MQ=255)

                               ggCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttat                                                                                                                     <  1:3324662/150‑1 (MQ=255)

                               ggCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttat                                                                                                                     <  1:3324656/150‑1 (MQ=255)

                               ggCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCatttat                                                                                                                     <  1:3324497/150‑1 (MQ=255)

                                gCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCatttatt                                                                                                                    <  1:1866858/150‑1 (MQ=255)

                                   gtttaTGGAGTTAATCCAGGGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTc                                                                                                                 <  1:578754/146‑1 (MQ=255)

                                    aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTcc                                                                                                                >  1:4060335/1‑150 (MQ=255)

                                    aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTcc                                                                                                                >  1:4466763/1‑150 (MQ=255)

                                      aaTGGAGTTAATACAGTGGTCATTTCAACTCTGATGTCAATAGCAGGTTTATGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCAtt                                                                                                              <  2:2062374/150‑1 (MQ=255)

                                        tGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTg                                                                                                            <  1:940569/150‑1 (MQ=255)

                                         ggAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGt                                                                                                           <  1:4288538/150‑1 (MQ=255)

                                         ggAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGt                                                                                                           <  2:3613421/150‑1 (MQ=255)

                                            gTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                                                                                                        <  2:4436756/150‑1 (MQ=255)

                                               aaTACAGTTGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                                                                                                     <  2:1226146/150‑1 (MQ=255)

                                               aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                                                                                                     <  2:786404/150‑1 (MQ=255)

                                               aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                                                                                                     <  2:226753/150‑1 (MQ=255)

                                                 tACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttt                                                                                                   >  1:4555838/1‑150 (MQ=255)

                                                  aCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAAACTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttttt                                                                                                  >  2:3105110/1‑150 (MQ=255)

                                                   cAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttttt                                                                                                 <  2:4579998/150‑1 (MQ=255)

                                                         tCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAttt                                                                                           >  1:867318/1‑150 (MQ=255)

                                                         tCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAtnt                                                                                           <  1:1177624/150‑1 (MQ=255)

                                                           aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                         <  2:2110158/150‑1 (MQ=255)

                                                           aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                         <  2:4359809/150‑1 (MQ=255)

                                                           aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                         <  2:370065/150‑1 (MQ=255)

                                                               cAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGa                                                                                     <  1:3436912/150‑1 (MQ=255)

                                                                aaCTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATACCTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGaa                                                                                    >  1:3541389/1‑150 (MQ=255)

                                                                    ctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAc                                                                                <  1:719516/150‑1 (MQ=255)

                                                                     tgatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAct                                                                               >  1:972419/1‑150 (MQ=255)

                                                                     tgatgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAct                                                                               <  1:731989/150‑1 (MQ=255)

                                                                     tgatgaCAATAGCAGGTTTAGGAACACCGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAct                                                                               >  1:973045/1‑150 (MQ=255)

                                                                          aCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttct                                                                          >  2:255532/1‑150 (MQ=255)

                                                                           cAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctc                                                                         >  1:4091380/1‑150 (MQ=255)

                                                                           cAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctc                                                                         >  1:4089620/1‑150 (MQ=255)

                                                                           cAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctc                                                                         <  2:2990814/150‑1 (MQ=255)

                                                                            aaTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATCACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctct                                                                        >  2:3730415/1‑150 (MQ=255)

                                                                             aTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctctt                                                                       <  2:571224/150‑1 (MQ=255)

                                                                                gCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTtata                                                                    <  1:2321458/150‑1 (MQ=255)

                                                                                   ggTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGa                                                                 >  1:2791418/1‑150 (MQ=255)

                                                                                     tttAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGAtt                                                               <  2:4692319/150‑1 (MQ=255)

                                                                                       tAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTAt                                                             >  2:1317413/1‑150 (MQ=255)

                                                                                        aGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATg                                                            >  2:1672616/1‑150 (MQ=255)

                                                                                           aaCACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtct                                                         <  1:647972/150‑1 (MQ=255)

                                                                                            acacTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtctc                                                        <  2:3668939/150‑1 (MQ=255)

                                                                                              acTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCtt                                                      >  1:2744206/1‑150 (MQ=255)

                                                                                                tGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTg                                                    >  1:1031501/1‑150 (MQ=255)

                                                                                                tGACAGGTACAATATTTTTTAGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                     <  2:1641409/150‑1 (MQ=255)

                                                                                                   catgTACAATATTTTTTGGTTTTGTTGGTTATAAGATTGTTTT‑AAAAAAAGCCTTTGTAGTCGGTCTAATATCTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGTATTATGTCTCTTTGGAt                                                 <  1:2885752/147‑1 (MQ=255)

                                                                                                       tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAAt                                             <  1:3946400/150‑1 (MQ=255)

                                                                                                        aCAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATg                                            <  2:2373401/150‑1 (MQ=255)

                                                                                                          aaTATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGtt                                          <  1:2162574/150‑1 (MQ=255)

                                                                                                           atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGttt                                         >  2:1797671/1‑150 (MQ=255)

                                                                                                           atatTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATTTCTCTTTGGATTAATtttt                                         >  2:70035/1‑150 (MQ=255)

                                                                                                               tttttGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACaa                                     >  1:325315/1‑150 (MQ=255)

                                                                                                                 tttGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAt                                   >  2:1157242/1‑150 (MQ=255)

                                                                                                                       tttGTTGGTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGt                             <  2:4441610/150‑1 (MQ=255)

                                                                                                                          gttgGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                          >  2:2508731/1‑150 (MQ=255)

                                                                                                                           ttgGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTg                         >  2:2032618/1‑150 (MQ=255)

                                                                                                                            tgGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCTGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGc                        <  2:4456683/150‑1 (MQ=255)

                                                                                                                              gTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAg                      <  1:4119313/150‑1 (MQ=255)

                                                                                                                              gTGATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAg                      >  2:1783069/1‑150 (MQ=255)

                                                                                                                                gATAAGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAggg                    >  2:4058470/1‑150 (MQ=255)

                                                                                                                                 aTAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggt                   <  2:4061142/150‑1 (MQ=255)

                                                                                                                                    aGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttgg                <  1:3980983/150‑1 (MQ=255)

                                                                                                                                    aGATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttgg                <  2:2803760/150‑1 (MQ=255)

                                                                                                                                     gATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggt               <  2:2955492/150‑1 (MQ=255)

                                                                                                                                     gATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggt               <  2:2554345/150‑1 (MQ=255)

                                                                                                                                     gATTGGTGT‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggt               <  1:3589855/150‑1 (MQ=255)

                                                                                                                                        tGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTcc            >  1:881953/1‑150 (MQ=255)

                                                                                                                                        tGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTcc            >  1:3272928/1‑150 (MQ=255)

                                                                                                                                         ggTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCa           <  2:4019585/150‑1 (MQ=255)

                                                                                                                                             t‑AAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAt       <  2:1953111/150‑1 (MQ=255)

                                                                                                                                               aaaaaaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAtt      <  2:3283909/150‑1 (MQ=255)

                                                                                                                                                aaaaaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATTGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAttt     <  1:128810/150‑1 (MQ=255)

                                                                                                                                                 aaaaaaCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTta    <  2:3613758/150‑1 (MQ=255)

                                                                                                                                                   aaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTtata  <  1:1483963/150‑1 (MQ=255)

GGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGT‑AAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATA  >  CP009273/4494572‑4494867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`