Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,512,693 T→A 92.4% K407* (AAG→TAG)  yjhG ← putative dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,512,6930TA92.4% 1060.9 / 56.4 314K407* (AAG→TAG) yjhGputative dehydratase
Reads supporting (aligned to +/- strand):  ref base T (9/15);  new base A (178/112);  total (187/127)
Fisher's exact test for biased strand distribution p-value = 2.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

TGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTG  >  CP009273/4512546‑4512841
                                                                                                                                                   |                                                                                                                                                    
      tGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCt                                                                                                                                              >  2:3389557/1‑150 (MQ=255)
       ggATTTGATCACCGAACCTTCTGGCGCACTATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTg                                                                                                                                             >  2:3946449/1‑150 (MQ=255)
        gATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGa                                                                                                                                            >  1:1340650/1‑150 (MQ=255)
        gATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGa                                                                                                                                            <  1:811397/150‑1 (MQ=255)
          tttGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACg                                                                                                                                          >  1:1739566/1‑150 (MQ=255)
              aTCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGt                                                                                                                                      >  1:3892400/1‑150 (MQ=255)
                cACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTc                                                                                                                                    <  1:3658834/150‑1 (MQ=255)
                 aCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCg                                                                                                                                   >  1:739342/1‑150 (MQ=255)
                   cGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGa                                                                                                                                 <  2:633788/150‑1 (MQ=255)
                   cGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGa                                                                                                                                 <  2:627369/150‑1 (MQ=255)
                    gAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAg                                                                                                                                <  1:3552307/150‑1 (MQ=255)
                     aaCCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGt                                                                                                                               >  2:782225/1‑150 (MQ=255)
                       ccTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGc                                                                                                                             >  1:1841656/1‑150 (MQ=255)
                       ccTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGc                                                                                                                             <  1:262294/150‑1 (MQ=255)
                       ccTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGc                                                                                                                             >  2:2020343/1‑150 (MQ=255)
                        cTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCt                                                                                                                            <  2:2115871/150‑1 (MQ=255)
                               cgcAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCccacca                                                                                                                     <  1:2362602/150‑1 (MQ=255)
                                gcAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAg                                                                                                                    <  1:2145408/150‑1 (MQ=255)
                                gcAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAg                                                                                                                    >  1:4542335/1‑150 (MQ=255)
                                   atatTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCg                                                                                                                 >  2:1798057/1‑150 (MQ=255)
                                   atatTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCg                                                                                                                 <  2:1353424/150‑1 (MQ=255)
                                     atTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAg                                                                                                               <  1:2188790/150‑1 (MQ=255)
                                      ttGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAgg                                                                                                              >  2:2081797/1‑150 (MQ=255)
                                      ttGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAgg                                                                                                              >  2:4080593/1‑150 (MQ=255)
                                       tGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGt                                                                                                             <  1:1080000/150‑1 (MQ=255)
                                             ccGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctt                                                                                                       <  2:2419166/150‑1 (MQ=255)
                                              cGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttcttt                                                                                                      >  2:2939910/1‑150 (MQ=255)
                                               gggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctttc                                                                                                     <  2:2149861/150‑1 (MQ=255)
                                               gggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctttc                                                                                                     <  1:4082681/150‑1 (MQ=255)
                                                ggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCa                                                                                                    <  2:2837044/150‑1 (MQ=255)
                                                 gAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAg                                                                                                   >  2:1825091/1‑150 (MQ=255)
                                                   aGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCg                                                                                                 >  2:3337467/1‑150 (MQ=255)
                                                      tGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgc                                                                                              >  1:1518545/1‑150 (MQ=255)
                                                       gATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgct                                                                                             <  1:3154093/150‑1 (MQ=255)
                                                       gATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgct                                                                                             >  1:3589316/1‑150 (MQ=255)
                                                       gATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgct                                                                                             >  2:3305206/1‑150 (MQ=255)
                                                       gATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgct                                                                                             <  1:4699065/150‑1 (MQ=255)
                                                        aTAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctg                                                                                            <  2:1721465/150‑1 (MQ=255)
                                                         tAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctgc                                                                                           >  2:3217001/1‑150 (MQ=255)
                                                         tAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctgc                                                                                           >  1:4234795/1‑150 (MQ=255)
                                                          aGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGcc                                                                                          >  2:1341060/1‑150 (MQ=255)
                                                          aGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGcc                                                                                          <  2:3741791/150‑1 (MQ=255)
                                                            ttGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCgg                                                                                        <  1:1189629/150‑1 (MQ=255)
                                                            ttGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCgg                                                                                        >  1:1802571/1‑150 (MQ=255)
                                                            ttGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCgg                                                                                        >  1:4206365/1‑150 (MQ=255)
                                                              gAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTa                                                                                      <  2:1652474/150‑1 (MQ=255)
                                                               aGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTaa                                                                                     >  1:2766987/1‑150 (MQ=255)
                                                                ggTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAAc                                                                                    >  1:1170853/1‑150 (MQ=255)
                                                                    aaTCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTc                                                                                <  2:3689749/150‑1 (MQ=255)
                                                                    aaTCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTc                                                                                >  1:3539305/1‑150 (MQ=255)
                                                                         gcgcgcTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAAc                                                                           >  2:3320912/1‑150 (MQ=255)
                                                                          cgcgcTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACg                                                                          <  1:1653749/150‑1 (MQ=255)
                                                                            cgcTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTc                                                                        >  2:436003/1‑150 (MQ=255)
                                                                             gcTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCt                                                                       >  1:3859813/1‑150 (MQ=255)
                                                                             gcTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCt                                                                       <  2:418977/150‑1 (MQ=255)
                                                                               ttttGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTc                                                                     >  1:4504168/1‑150 (MQ=255)
                                                                               ttttGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTc                                                                     <  1:2905218/150‑1 (MQ=255)
                                                                                 ttgcttgcTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCAt                                                                   >  1:1431423/1‑150 (MQ=255)
                                                                                 ttgcttgcTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCAt                                                                   >  1:2521471/1‑150 (MQ=255)
                                                                                 gtgcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCAt                                                                   <  1:2841396/149‑1 (MQ=255)
                                                                                  tgcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATg                                                                  >  1:2433898/1‑150 (MQ=255)
                                                                                  tgcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATg                                                                  <  2:4260390/150‑1 (MQ=255)
                                                                                     ttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACAGTCATAACGTCTTCATGcaa                                                               >  2:3594206/1‑150 (MQ=255)
                                                                                       gctgcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaaca                                                             <  2:2816071/150‑1 (MQ=255)
                                                                                       gctgcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaaca                                                             <  2:424208/150‑1 (MQ=255)
                                                                                        ctgcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaacaa                                                            >  1:1121312/1‑150 (MQ=255)
                                                                                          gcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATc                                                          <  1:4754804/150‑1 (MQ=255)
                                                                                           cGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATcc                                                         >  1:4050339/1‑150 (MQ=255)
                                                                                                 cATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCt                                                   >  2:3910788/1‑150 (MQ=255)
                                                                                                   tGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTgc                                                 >  1:4026308/1‑150 (MQ=255)
                                                                                                    gATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTgcg                                                >  2:1075370/1‑150 (MQ=255)
                                                                                                    gATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTgcg                                                >  2:2289122/1‑150 (MQ=255)
                                                                                                        aCTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGa                                            >  1:82206/1‑150 (MQ=255)
                                                                                                           tCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGc                                         >  1:2255854/1‑150 (MQ=255)
                                                                                                           tCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGc                                         >  1:4656447/1‑150 (MQ=255)
                                                                                                              tCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAAc                                      <  2:261306/150‑1 (MQ=255)
                                                                                                               cAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACa                                     >  1:2895448/1‑150 (MQ=255)
                                                                                                                    ttGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACt                                >  2:1694892/1‑150 (MQ=255)
                                                                                                                    ttGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACt                                >  2:3730534/1‑150 (MQ=255)
                                                                                                                    ttGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCAGCCGGTAACCGTCATAACGTCTACATGCAACAATCCGAGGCTGCGCACAAGCAACATGAca                                >  2:125705/1‑149 (MQ=255)
                                                                                                                     tGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACtt                               >  2:4495042/1‑150 (MQ=255)
                                                                                                                     tGATTTGTTCCTGATCGAGCAGGAGTGGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACtt                               >  2:3368373/1‑150 (MQ=255)
                                                                                                                       aTTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTcc                             >  1:1603592/1‑150 (MQ=255)
                                                                                                                       aTTAGTTCCTGATCGATCAGGAGTTGCTTGAACCGCTGACGCCGATCTGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTTCTGCCGGTAACCGACATAAAGTCATCATTCAACAAACCGAGGCTGCGCAGATGCAACATGACttac                             >  2:15612/1‑148 (MQ=255)
                                                                                                                        tttGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCg                            <  2:314055/150‑1 (MQ=255)
                                                                                                                          tGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGc                          >  2:700679/1‑150 (MQ=255)
                                                                                                                           gTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGca                         >  2:886183/1‑150 (MQ=255)
                                                                                                                            ttCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGcac                        >  2:3859749/1‑150 (MQ=255)
                                                                                                                            ttCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGcac                        <  1:4013584/150‑1 (MQ=255)
                                                                                                                              ccTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCacac                      <  2:4595331/150‑1 (MQ=255)
                                                                                                                               cTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcacc                     >  2:1005238/1‑150 (MQ=255)
                                                                                                                               cTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcacc                     >  2:2745677/1‑150 (MQ=255)
                                                                                                                                  aTCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACaccacc                  >  1:374679/1‑150 (MQ=255)
                                                                                                                                    cGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTg                <  1:3389557/150‑1 (MQ=255)
                                                                                                                                    cGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTg                >  2:534742/1‑150 (MQ=255)
                                                                                                                                        cAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCAt            >  1:4572341/1‑150 (MQ=255)
                                                                                                                                        cAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCAt            <  2:4322039/150‑1 (MQ=255)
                                                                                                                                         aGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATa           <  1:3188405/150‑1 (MQ=255)
                                                                                                                                           gAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATaaa         <  2:1984995/150‑1 (MQ=255)
                                                                                                                                              ttGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGc      <  1:1050639/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCATTG  >  CP009273/4512546‑4512841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑