| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 361,806 | G→A | 100% | intergenic (‑63/+14) | lacI ← / ← mhpR | DNA‑binding transcriptional repressor/mhp operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 361,806 | 0 | G | A | 95.4% | 21681.9 / inf | 6166 | intergenic (‑63/+14) | lacI/mhpR | DNA‑binding transcriptional repressor/mhp operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (149/135); new base A (2833/3039); total (2985/3181) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 6172 total aligned reads displayed.
GTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTC > CP009273/361657‑361955 | ggctggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGcc > 1:4215655/1‑150 (MQ=255) ggctggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCAcc > 1:435636/1‑150 (MQ=255) tggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt > 1:4445606/1‑150 (MQ=255) ccTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGa < 2:2907425/150‑1 (MQ=255) cTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAt > 2:1931138/1‑150 (MQ=255) ggTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATgg < 2:88569/150‑1 (MQ=255) gTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGgt > 2:3886838/1‑150 (MQ=255) gTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgt < 2:4667510/150‑1 (MQ=255) ttCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtg < 2:4212073/150‑1 (MQ=255) caccacGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTc > 1:3299243/1‑150 (MQ=255) accacGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCa > 1:940429/1‑150 (MQ=255) cACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAt > 2:2471253‑M2/1‑149 (MQ=255) cACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAGTCGATGGTGTCAAt < 1:1425425‑M2/150‑2 (MQ=255) cgGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaat < 1:1900993‑M2/150‑6 (MQ=255) gggAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatt > 2:718174‑M2/1‑144 (MQ=255) gAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAatg < 1:2463897/150‑1 (MQ=255) aaaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaa > 2:2028368‑M2/1‑141 (MQ=255) aaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaag < 1:3920843‑M2/150‑11 (MQ=255) aaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaag > 1:18232‑M2/1‑140 (MQ=255) aaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaag > 1:861063‑M2/1‑140 (MQ=255) ggTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggca > 1:3995759‑M2/1‑137 (MQ=255) ggTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggca > 1:1263644‑M2/1‑137 (MQ=255) ggTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGGGTCAAttaattaaaggca < 1:4655773‑M2/150‑14 (MQ=255) gTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcat < 1:660722‑M2/150‑15 (MQ=255) gTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcat > 2:1827816‑M2/1‑136 (MQ=255) tCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatc < 2:541632‑M2/150‑16 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatca > 2:3163895‑M2/1‑134 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatca < 1:386456‑M2/150‑17 (MQ=255) tGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTCTCATGCCATACCGCGAAAGGTTTTGCACCAGTCGATGGTGTCAAgtaattaaaggcatcaa < 1:1335660‑M2/150‑18 (MQ=255) gATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaa < 2:3799530‑M2/150‑19 (MQ=255) aTAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTc < 1:2620949/150‑1 (MQ=255) aTAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaat > 1:2395200‑M2/1‑131 (MQ=255) tAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaata > 1:2222477‑M2/1‑130 (MQ=255) tAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaata > 2:3712020‑M2/1‑130 (MQ=255) aaGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataa < 1:4567533‑M2/150‑22 (MQ=255) agagACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaa > 1:1586346‑M2/1‑128 (MQ=255) gagaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaa > 1:3042290‑M2/1‑127 (MQ=255) agaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaac > 1:287355‑M2/1‑126 (MQ=255) agaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaac > 2:3556897‑M2/1‑126 (MQ=255) acacCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTcc < 1:2150271/150‑1 (MQ=255) cacCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaa < 1:1087464‑M2/150‑28 (MQ=255) ggCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAGGGTGTCAAttaattaaaggcatcaaataaaacgaaaggc < 2:2381566‑M2/150‑32 (MQ=255) aTACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGccacca > 2:4127875/1‑150 (MQ=255) aTACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGccacca > 1:3775007/1‑150 (MQ=255) tACTCTGCTACATCGTATAACGTTACTGGTTTAACATTCACCACCCTTAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCTTTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcag > 1:3217663‑M2/1‑115 (MQ=255) tACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcag < 1:3707790‑M2/150‑36 (MQ=255) aCTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCCTTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagt < 2:61462‑M2/150‑37 (MQ=255) aCTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagt > 2:1033817‑M2/1‑114 (MQ=255) tctGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcg > 1:3458298‑M2/1‑112 (MQ=255) ctGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcga > 2:2120677‑M2/1‑111 (MQ=255) cGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaag < 2:809760‑M2/150‑43 (MQ=255) gACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggttcagtcgaaaga < 1:3533719‑M2/150‑44 (MQ=255) gACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaaga < 1:763300‑M2/150‑44 (MQ=255) cATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagact > 1:2065164‑M2/1‑105 (MQ=255) tCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgg > 2:4459931‑M2/1‑103 (MQ=255) ataACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct < 2:3645295‑M2/150‑52 (MQ=255) ataACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagacggggcgt < 2:4042655‑M2/150‑52 (MQ=255) taACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt > 2:1351566‑M2/1‑98 (MQ=255) taACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt > 1:2950084‑M2/1‑98 (MQ=255) taACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt < 1:4295948‑M2/150‑53 (MQ=255) aaCGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt < 2:3242270‑M2/150‑54 (MQ=255) cGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg > 2:3081212‑M2/1‑95 (MQ=255) ttACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt > 1:1743752‑M2/1‑93 (MQ=255) ttACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt > 1:1975722‑M2/1‑93 (MQ=255) tACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAtgaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctgtcgttt < 1:4132038‑M2/150‑59 (MQ=255) aCTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcaggcgaaagactgggcctttcgtttt < 1:279101‑M2/150‑60 (MQ=255) tGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat > 2:2986294‑M2/1‑89 (MQ=255) gTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct < 1:2297176‑M2/150‑64 (MQ=255) tCACATTCACCACCCTGAATTGACTCTCGTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt > 2:2723683‑M2/1‑84 (MQ=255) cacaTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaccggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg > 1:4054642‑M2/1‑83 (MQ=255) caTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt < 2:173407‑M2/150‑70 (MQ=255) caTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt < 1:4725361‑M2/150‑70 (MQ=255) ttCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCAAACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaaagaaaggctcagtcgaaagactgggcctttcgttttatatgttgtttg < 1:492641‑M2/150‑72 (MQ=255) caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaacggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc > 2:1136387‑M2/1‑77 (MQ=255) caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc > 2:3447904‑M2/1‑77 (MQ=255) ccaccCTGAATTGACTCTCTTCCGGGCGCTCTCAGGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg > 1:560168‑M2/1‑75 (MQ=255) ccaccCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg < 2:2636569‑M2/150‑76 (MQ=255) accCGGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAtgacttaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttgcgttttatctgttgtttgtcggtg < 2:4302867‑M2/150‑78 (MQ=255) ccTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTAccc > 2:1544063/1‑150 (MQ=255) ccTGAATTGACTCTCTGCCGGGCGCTATCATGCCATACCGCGAAAGGTTTGGCACCATTCGATGGTGTCAAttaattaaaggcatcaaagacaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa > 2:4574064‑M2/1‑71 (MQ=255) cTGACGTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaccgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac > 2:1656312‑M2/1‑70 (MQ=255) cTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac > 2:3353320‑M2/1‑70 (MQ=255) agaATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg > 1:2545328‑M2/2‑69 (MQ=255) aTTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc > 2:221446‑M2/1‑66 (MQ=255) aTTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc < 1:1002095‑M2/150‑85 (MQ=255) aCTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcct < 1:1168604‑M2/150‑89 (MQ=255) ctctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctg < 1:1499780‑M2/150‑90 (MQ=255) tctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGcc > 2:1228733/1‑150 (MQ=255) tctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctga < 1:3375391‑M2/150‑91 (MQ=255) ccGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtagga > 1:1670786‑M2/1‑54 (MQ=255) ggCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaa < 2:2559512‑M2/150‑100 (MQ=255) ggCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaa > 1:2711363‑M2/1‑51 (MQ=255) gcTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtgggttgtcggtgaacgctctcctgagtaggacaaatc < 2:4748828‑M2/150‑103 (MQ=255) tCATGCCATACCGCGAAAGGTTTTGAACCATTCGATGGTGTCAAttaattacaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgcc > 1:3617717‑M2/1‑44 (MQ=255) ccATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccct > 2:281089‑M2/1‑39 (MQ=255) cATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgcccta > 2:3968993‑M2/1‑38 (MQ=255) cATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgcccta < 1:3130929‑M2/150‑113 (MQ=255) cATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgcccta < 2:2298233‑M2/150‑113 (MQ=255) aTACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctag > 2:4380097‑M2/1‑37 (MQ=255) aTACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctag < 2:1458671‑M2/150‑114 (MQ=255) | GTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTC > CP009273/361657‑361955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |