BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,590,074	A→C	27.5%	V240G (GTA→GGA)	dnaC ←	DNA biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,590,074	0	A	C	27.5%	392.5 / 251.8	265	V240G (GTA→GGA)	dnaC	DNA biosynthesis protein
Reads supporting (aligned to +/- strand): ref base A (103/89); new base C (42/31); total (145/120)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

AGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCG  >  CP009273/4589925‑4590222

aGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTa                                                                                                                                                      >  2:876897/1‑150 (MQ=255)

   aCGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTcccc                                                                                                                                                   <  2:3171521/150‑1 (MQ=255)

   aCGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTAccc                                                                                                                                                   <  2:140477/150‑1 (MQ=255)

       agcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCt                                                                                                                                               >  1:1001052/1‑150 (MQ=255)

       agcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCt                                                                                                                                               <  1:1524653/150‑1 (MQ=255)

        gcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTa                                                                                                                                              >  1:4763071/1‑150 (MQ=255)

          aTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACg                                                                                                                                            >  2:2706066/1‑150 (MQ=255)

           tGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACgg                                                                                                                                           <  1:2495818/150‑1 (MQ=255)

              aCCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAg                                                                                                                                        >  2:3740299/1‑150 (MQ=255)

              aCCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAg                                                                                                                                        >  2:574521/1‑150 (MQ=255)

                     tGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATccc                                                                                                                                 >  1:4366025/1‑150 (MQ=255)

                      ggAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCa                                                                                                                                <  1:4351272/150‑1 (MQ=255)

                      ggAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCa                                                                                                                                >  2:795579/1‑150 (MQ=255)

                         aaTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGtt                                                                                                                             >  2:3259839/1‑150 (MQ=255)

                          aTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTg                                                                                                                            >  1:3356640/1‑150 (MQ=255)

                            tAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGaa                                                                                                                          >  1:3389126/1‑150 (MQ=255)

                             aaTGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAg                                                                                                                         <  2:3548196/150‑1 (MQ=255)

                                gcgcTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATc                                                                                                                      <  1:3285389/150‑1 (MQ=255)

                                        ggcAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACaa                                                                                                              >  2:2665341/1‑150 (MQ=255)

                                        ggcAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACaa                                                                                                              >  1:387127/1‑150 (MQ=255)

                                            aTGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTg                                                                                                          <  2:3109804/150‑1 (MQ=255)

                                             tGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGt                                                                                                         <  2:901858/150‑1 (MQ=255)

                                             tGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGt                                                                                                         >  1:2044606/1‑150 (MQ=255)

                                              ggCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGtt                                                                                                        <  2:3815641/150‑1 (MQ=255)

                                                cacagcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAc                                                                                                      <  2:3399289/150‑1 (MQ=255)

                                                cacagcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAc                                                                                                      >  2:4192712/1‑150 (MQ=255)

                                                   agcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCa                                                                                                   >  1:274461/1‑150 (MQ=255)

                                                   agcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCa                                                                                                   <  2:3827832/150‑1 (MQ=255)

                                                     cacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAgg                                                                                                 >  1:4656683/1‑150 (MQ=255)

                                                      acagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGgc                                                                                                >  1:2872997/1‑150 (MQ=255)

                                                      acagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGgc                                                                                                >  2:4506346/1‑150 (MQ=255)

                                                        agaagaTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGgcgc                                                                                              >  2:4574289/1‑150 (MQ=255)

                                                          aagaTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCAt                                                                                            <  1:2460823/150‑1 (MQ=255)

                                                            gaTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATAc                                                                                          >  1:868347/1‑150 (MQ=255)

                                                             aTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACg                                                                                         >  2:718364/1‑150 (MQ=255)

                                                               gTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGt                                                                                       >  2:164390/1‑150 (MQ=255)

                                                               gTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGt                                                                                       >  1:3902535/1‑150 (MQ=255)

                                                                    aCAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATc                                                                                  <  1:3687657/150‑1 (MQ=255)

                                                                      aGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAc                                                                                <  2:2490704/150‑1 (MQ=255)

                                                                      aGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAc                                                                                <  2:2400584/150‑1 (MQ=255)

                                                                        ttttCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAcgc                                                                              >  1:3755532/1‑150 (MQ=255)

                                                                         tttCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAcgcg                                                                             <  2:1359804/150‑1 (MQ=255)

                                                                            catcatcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTc                                                                          <  1:2529321/150‑1 (MQ=255)

                                                                              tcatcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGc                                                                        >  2:4676098/1‑150 (MQ=255)

                                                                               catcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGcc                                                                       >  1:2415080/1‑150 (MQ=255)

                                                                               catcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGcc                                                                       >  2:2815578/1‑150 (MQ=255)

                                                                               catcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGcc                                                                       >  2:148747/1‑150 (MQ=255)

                                                                                atcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGccc                                                                      >  1:3247110/1‑150 (MQ=255)

                                                                                   atTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGc                                                                   >  2:4008479/1‑150 (MQ=255)

                                                                                    ttACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCa                                                                  <  2:1205347/150‑1 (MQ=255)

                                                                                     tACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCaa                                                                 >  1:1051940/1‑150 (MQ=255)

                                                                                     tACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCaa                                                                 <  1:4229627/150‑1 (MQ=255)

                                                                                       cTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACt                                                               >  2:744890/1‑150 (MQ=255)

                                                                                        tCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACtt                                                              <  2:2686590/150‑1 (MQ=255)

                                                                                         cAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTg                                                             >  1:2172460/1‑150 (MQ=255)

                                                                                         cAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTg                                                             <  2:2317387/150‑1 (MQ=255)

                                                                                          aaGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTgg                                                            <  1:2621662/150‑1 (MQ=255)

                                                                                          aaGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTgg                                                            <  1:2493963/150‑1 (MQ=255)

                                                                                            ggTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTc                                                          >  2:1672935/1‑150 (MQ=255)

                                                                                              tGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAt                                                        >  1:1334131/1‑150 (MQ=255)

                                                                                              tGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAt                                                        <  1:965149/150‑1 (MQ=255)

                                                                                                gAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAtct                                                      <  1:3507939/150‑1 (MQ=255)

                                                                                                gAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAtct                                                      <  1:3819361/150‑1 (MQ=255)

                                                                                                   ttGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCtt                                                   <  2:181223/150‑1 (MQ=255)

                                                                                                   ttGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCtt                                                   >  1:1624323/1‑150 (MQ=255)

                                                                                                      tgtCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCa                                                >  2:372006/1‑150 (MQ=255)

                                                                                                        tCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCata                                              <  2:3597370/150‑1 (MQ=255)

                                                                                                        tCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCata                                              >  2:1069781/1‑150 (MQ=255)

                                                                                                         cGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCatat                                             >  1:2911706/1‑150 (MQ=255)

                                                                                                          gCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATAtt                                            <  1:83187/150‑1 (MQ=255)

                                                                                                           cAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTa                                           <  2:4064457/150‑1 (MQ=255)

                                                                                                              tataCCGCCAGAAACGGGGATTAATACTCTGTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTg                                        <  2:4163012/150‑1 (MQ=255)

                                                                                                               ataCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGt                                       >  1:573683/1‑150 (MQ=255)

                                                                                                               ataCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGt                                       >  2:1297774/1‑150 (MQ=255)

                                                                                                               ataCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGt                                       <  2:1265619/150‑1 (MQ=255)

                                                                                                                taCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGtt                                      <  1:1933292/150‑1 (MQ=255)

                                                                                                                 aCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTg                                     <  2:1883254/150‑1 (MQ=255)

                                                                                                                  ccGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTgg                                    <  1:3306176/150‑1 (MQ=255)

                                                                                                                    gCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTc                                  >  2:2572944/1‑150 (MQ=255)

                                                                                                                     ccAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCa                                 <  2:2861094/150‑1 (MQ=255)

                                                                                                                     ccAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCa                                 <  2:4352940/150‑1 (MQ=255)

                                                                                                                         aaaCGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCAt                             <  2:387127/150‑1 (MQ=255)

                                                                                                                            cGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATccc                          <  1:3905287/150‑1 (MQ=255)

                                                                                                                             ggggATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCg                         >  2:1598350/1‑150 (MQ=255)

                                                                                                                              gggATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCgg                        >  2:614518/1‑150 (MQ=255)

                                                                                                                              gggATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCgg                        >  1:3373671/1‑150 (MQ=255)

                                                                                                                                  ttAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCCCAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTgg                    <  2:3989863/150‑1 (MQ=255)

                                                                                                                                  ttAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTgg                    >  1:4528071/1‑150 (MQ=255)

                                                                                                                                   tAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTggg                   >  2:1285724/1‑150 (MQ=255)

                                                                                                                                   tAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTggg                   >  1:4077316/1‑150 (MQ=255)

                                                                                                                                    aaTACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGc                  >  1:3015742/1‑150 (MQ=255)

                                                                                                                                    aaTACTCTTGACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGc                  <  1:774180/150‑1 (MQ=255)

                                                                                                                                      tACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGt                >  2:1579820/1‑150 (MQ=255)

                                                                                                                                          ctTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTgg            <  1:1742080/150‑1 (MQ=255)

                                                                                                                                           tttACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGga           <  2:2044606/150‑1 (MQ=255)

                                                                                                                                           tttACCTGGTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGga           <  2:293030/150‑1 (MQ=255)

                                                                                                                                            ttACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTACATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaa          >  2:598613/1‑150 (MQ=255)

                                                                                                                                              aNNTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaaga        >  2:2475823/1‑150 (MQ=255)

                                                                                                                                               ccTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATATCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaagaa       <  1:1839665/150‑1 (MQ=255)

                                                                                                                                                    tACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAgcg  <  2:4763071/150‑1 (MQ=255)

AGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCG  >  CP009273/4589925‑4590222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`