Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 385,815 G→T 50.9% pseudogene (109/1458 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273385,8150GT50.9% 42.1 / inf 204pseudogene (109/1458 nt)yaiTpseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base G (59/41);  new base T (63/41);  total (122/82)
Fisher's exact test for biased strand distribution p-value = 8.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 207 total aligned reads displayed.

TTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCA  >  CP009273/385666‑385962
                                                                                                                                                     |                                                                                                                                                   
ttGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCg                                                                                                                                                     <  2:1728332/150‑1 (MQ=255)
   aTGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCtata                                                                                                                                                  >  2:2566320/1‑150 (MQ=255)
   aTGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATa                                                                                                                                                  >  1:928171/1‑150 (MQ=255)
      gATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATc                                                                                                                                               <  2:3556984/150‑1 (MQ=255)
      gATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATACAGCAAACTATGATACCTGGACTTATATCGATAATc                                                                                                                                               <  2:1216109/150‑1 (MQ=255)
           aCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGtt                                                                                                                                          <  2:1406297/150‑1 (MQ=255)
           aCTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGtt                                                                                                                                          >  2:2668494/1‑150 (MQ=255)
              tattaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACa                                                                                                                                       >  1:295284/1‑150 (MQ=255)
               attaCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAg                                                                                                                                      >  1:974428/1‑150 (MQ=255)
                 taCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCTCAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCa                                                                                                                                    >  1:338773/1‑150 (MQ=255)
                 taCGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCa                                                                                                                                    <  1:1187725/150‑1 (MQ=255)
                    gTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACtt                                                                                                                                 <  1:4036445/150‑1 (MQ=255)
                      cccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGa                                                                                                                               <  1:2440770/150‑1 (MQ=255)
                       ccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGAt                                                                                                                              >  2:24914/1‑150 (MQ=255)
                       ccTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGAt                                                                                                                              >  1:884502/1‑150 (MQ=255)
                         tGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTg                                                                                                                            <  2:4477215/150‑1 (MQ=255)
                                ggATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCat                                                                                                                     >  2:4231148/1‑150 (MQ=255)
                                 gATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCata                                                                                                                    >  2:255111/1‑150 (MQ=255)
                                    gACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATgg                                                                                                                 >  2:832377/1‑150 (MQ=255)
                                    gACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATgg                                                                                                                 >  1:3107681/1‑150 (MQ=255)
                                     aCAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGa                                                                                                                >  2:3772695/1‑150 (MQ=255)
                                      cAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGAt                                                                                                               <  1:4562153/150‑1 (MQ=255)
                                      cAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGAt                                                                                                               <  2:3049761/150‑1 (MQ=255)
                                       aaaTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATa                                                                                                              <  1:3652110/150‑1 (MQ=255)
                                       aaaTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATa                                                                                                              >  2:1053237/1‑150 (MQ=255)
                                       aaaTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATa                                                                                                              >  1:1344220/1‑150 (MQ=255)
                                       aaaTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATa                                                                                                              >  1:1344056/1‑150 (MQ=255)
                                         aTGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAag                                                                                                            >  1:4368238/1‑150 (MQ=255)
                                           gCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAaggc                                                                                                          >  2:1189792/1‑150 (MQ=255)
                                                  tGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACt                                                                                                   <  1:1305459/150‑1 (MQ=255)
                                                     aaaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTa                                                                                                >  2:491926/1‑150 (MQ=255)
                                                      aaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAg                                                                                               >  2:2129143/1‑150 (MQ=255)
                                                      aaaaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAg                                                                                               >  2:2662625/1‑150 (MQ=255)
                                                        aaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAt                                                                                             <  1:2343277/150‑1 (MQ=255)
                                                        aaGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGAt                                                                                             <  2:4109929/150‑1 (MQ=255)
                                                         aGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATg                                                                                            >  1:100881/1‑150 (MQ=255)
                                                         aGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATg                                                                                            <  1:4568430/150‑1 (MQ=255)
                                                              cTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAAc                                                                                       <  1:110620/150‑1 (MQ=255)
                                                               ttGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACt                                                                                      <  1:425604/150‑1 (MQ=255)
                                                                tGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTa                                                                                     >  1:1901148/1‑150 (MQ=255)
                                                                  tagtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATg                                                                                   >  2:4723651/1‑150 (MQ=255)
                                                                  tagtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATg                                                                                   >  1:1398804/1‑150 (MQ=255)
                                                                    gtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTa                                                                                 <  2:2894257/150‑1 (MQ=255)
                                                                    gtaTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTa                                                                                 >  2:1949604/1‑150 (MQ=255)
                                                                     taTCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTaa                                                                                <  2:884485/150‑1 (MQ=255)
                                                                       tCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAAc                                                                              >  1:3058626/1‑150 (MQ=255)
                                                                        cacaATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACt                                                                             >  1:1376324/1‑150 (MQ=255)
                                                                          caATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACtat                                                                           >  2:4312869/1‑150 (MQ=255)
                                                                             ttAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGt                                                                        <  1:3300056/150‑1 (MQ=255)
                                                                                gCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGt                                                                     >  2:2216225/1‑150 (MQ=255)
                                                                                 cATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGtt                                                                    <  1:2373961/150‑1 (MQ=255)
                                                                                  aTTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGttt                                                                   <  1:1647511/150‑1 (MQ=255)
                                                                                  aTTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGttt                                                                   >  1:3950126/1‑150 (MQ=255)
                                                                                       cTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCt                                                              >  1:4401595/1‑150 (MQ=255)
                                                                                            aCTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTac                                                         >  2:1365107/1‑150 (MQ=255)
                                                                                            aCTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTac                                                         >  2:2733409/1‑150 (MQ=255)
                                                                                              tctGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaa                                                       <  1:1288636/150‑1 (MQ=255)
                                                                                                 ggCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaacaa                                                    >  2:2880384/1‑150 (MQ=255)
                                                                                                  gCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTacaacaac                                                   >  2:3848756/1‑150 (MQ=255)
                                                                                                     aTCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACAcc                                                >  2:1373335/1‑150 (MQ=255)
                                                                                                       cACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCaa                                              >  1:1378969/1‑150 (MQ=255)
                                                                                                       cACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCaa                                              >  2:3238247/1‑150 (MQ=255)
                                                                                                        aCCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAAt                                             >  2:3846920/1‑150 (MQ=255)
                                                                                                          ctctCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATgg                                           >  1:859039/1‑150 (MQ=255)
                                                                                                            ctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTg                                         <  2:2780291/150‑1 (MQ=255)
                                                                                                            ctcAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTg                                         <  2:2273290/150‑1 (MQ=255)
                                                                                                               aGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAtt                                      >  2:3711607/1‑150 (MQ=255)
                                                                                                                ggCTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAttt                                     <  1:4696462/150‑1 (MQ=255)
                                                                                                                ggCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGAttt                                     <  2:1671082/150‑1 (MQ=255)
                                                                                                                  cTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCg                                   >  2:4427996/1‑150 (MQ=255)
                                                                                                                  cTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCg                                   <  1:671023/150‑1 (MQ=255)
                                                                                                                  cTAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCg                                   >  2:2989580/1‑150 (MQ=255)
                                                                                                                   tAATGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGa                                  <  2:1829011/150‑1 (MQ=255)
                                                                                                                      tGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATCGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCa                               >  2:561912/1‑150 (MQ=255)
                                                                                                                      tGCAGCAAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCa                               >  2:2952373/1‑150 (MQ=255)
                                                                                                                         agcaAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTc                            >  2:584773/1‑150 (MQ=255)
                                                                                                                         agcaAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTc                            >  2:2788666/1‑150 (MQ=255)
                                                                                                                         agcaAACTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTc                            >  2:399537/1‑150 (MQ=255)
                                                                                                                         agcaAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTc                            <  2:209462/150‑1 (MQ=255)
                                                                                                                            aaaCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCtt                         >  1:3787277/1‑150 (MQ=255)
                                                                                                                            aaaCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCtt                         >  1:334694/1‑150 (MQ=255)
                                                                                                                             aaCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCttt                        >  1:293811/1‑150 (MQ=255)
                                                                                                                              aCTATGATACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTa                       <  1:4483858/150‑1 (MQ=255)
                                                                                                                                  tGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACgg                   <  2:4073936/150‑1 (MQ=255)
                                                                                                                                    aTACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCg                 >  1:4695158/1‑150 (MQ=255)
                                                                                                                                    aTACCTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCg                 <  1:2129143/150‑1 (MQ=255)
                                                                                                                                     tACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGa                >  1:2009862/1‑150 (MQ=255)
                                                                                                                                       ccTGTAATTATATCTATAATCCCGTTACTGACCTTGATTGGGATCATATGGATAAGGCAGGCACTTTAGATGGCAAATATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCATTGGTGATTTCGATCAGTCCTTTAACGGCGATa              <  1:2778374/150‑1 (MQ=255)
                                                                                                                                       ccTGGACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATa              <  2:241298/150‑1 (MQ=255)
                                                                                                                                           gACTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGt          >  2:4589799/1‑150 (MQ=255)
                                                                                                                                            aCTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGtt         <  1:1376441/150‑1 (MQ=255)
                                                                                                                                             cTTATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTATATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTa        <  2:3201008/150‑1 (MQ=255)
                                                                                                                                              ttATATCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTaa       <  1:3845965/150‑1 (MQ=255)
                                                                                                                                              ttATATCGATACTCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTaa       <  1:4312869/150‑1 (MQ=255)
                                                                                                                                              ttATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTaa       <  1:2641007/150‑1 (MQ=255)
                                                                                                                                               tataTCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAAc      <  1:1970661/150‑1 (MQ=255)
                                                                                                                                                atatCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTATATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACg     <  1:24914/150‑1 (MQ=255)
                                                                                                                                                atatCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACg     >  2:3629685/1‑150 (MQ=255)
                                                                                                                                                  atCTATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGc   >  1:897820/1‑150 (MQ=255)
                                                                                                                                                   tCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCa  >  1:886002/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTGATGGATCAACTTATTACGTCCCTGAGGAGGGATGACAAATGCACTCCTGGAAAAAGAAACTTGTAGTATCACAATTAGCATTGGCTTGCACTCTGGCTATCACCTCTCAGGCTAATGCAGCAAACTATGATACCTGGACTTATATCGATAATCCCGTTACAGCACTTGATTGGGATCATATGGATAAGGCAGGCACTGTAGATGGCAACTATGTAAACTATAGTGGTTTTGTCTATTACAACAACACCAATGGTGATTTCGATCAGTCCTTTAACGGCGATACCGTTAACGGCA  >  CP009273/385666‑385962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑