Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 396,883 A→G 85.2% Q14Q (CAA→CAG)  iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273396,8830AG85.2% 603.5 / 77.3 209Q14Q (CAA→CAG) iraPanti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base A (13/18);  new base G (113/65);  total (126/83)
Fisher's exact test for biased strand distribution p-value = 2.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

ATTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  >  CP009273/396736‑397031
                                                                                                                                                   |                                                                                                                                                    
    gcgcAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAaagaa                                                                                                                                                <  2:3296370/150‑1 (MQ=255)
      gcAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGAGAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaagaaga                                                                                                                                              >  2:4505531/1‑150 (MQ=255)
        aaaGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaagaagaag                                                                                                                                            >  2:4328270/1‑150 (MQ=255)
            tATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTc                                                                                                                                        >  2:2151416/1‑150 (MQ=255)
              tttCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGa                                                                                                                                      >  2:2607801/1‑150 (MQ=255)
              tttCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGa                                                                                                                                      <  2:3272789/150‑1 (MQ=255)
              tttCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGa                                                                                                                                      >  1:4331886/1‑150 (MQ=255)
                tCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGaaa                                                                                                                                    >  2:2095865/1‑150 (MQ=255)
                tCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGaaa                                                                                                                                    >  2:2095554/1‑150 (MQ=255)
                   tttGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGaa                                                                                                                                 >  2:3455228/1‑150 (MQ=255)
                    ttGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAAc                                                                                                                                <  2:275419/150‑1 (MQ=255)
                     tGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACt                                                                                                                               <  1:3909429/150‑1 (MQ=255)
                      gTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtg                                                                                                                              >  1:4535097/1‑150 (MQ=255)
                      gTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtg                                                                                                                              >  2:2321119/1‑150 (MQ=255)
                      gTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtg                                                                                                                              >  1:832213/1‑150 (MQ=255)
                                  ataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTa                                                                                                                  <  2:4677520/150‑1 (MQ=255)
                                  ataATTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTa                                                                                                                  <  2:4677786/150‑1 (MQ=255)
                                   taCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAg                                                                                                                 <  2:2476658/150‑1 (MQ=255)
                                   taCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAg                                                                                                                 >  2:381378/1‑150 (MQ=255)
                                    aCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGa                                                                                                                <  2:1424368/150‑1 (MQ=255)
                                      ttCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAg                                                                                                              >  1:2240676/1‑150 (MQ=255)
                                       tCCAGACACTATGAAGTTGTGAAACATACTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                                                             <  1:3045417/150‑1 (MQ=255)
                                       tCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                                                             <  1:1067477/150‑1 (MQ=255)
                                          aGACACTATGAAGTTGTTAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCttt                                                                                                          <  1:4670597/150‑1 (MQ=255)
                                           gACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTg                                                                                                         >  2:4272666/1‑150 (MQ=255)
                                            acacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGATGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTgg                                                                                                        <  2:2229168/150‑1 (MQ=255)
                                            acacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTgg                                                                                                        <  2:3369760/150‑1 (MQ=255)
                                             cacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                                                       <  2:2915985/150‑1 (MQ=255)
                                             cacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                                                       <  2:2915995/150‑1 (MQ=255)
                                               cTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgaga                                                                                                     >  1:3729422/1‑150 (MQ=255)
                                                tATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                                                    >  2:348575/1‑150 (MQ=255)
                                                tATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                                                    >  2:347371/1‑150 (MQ=255)
                                                 aTGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAtt                                                                                                   >  2:3115506/1‑150 (MQ=255)
                                                 aTGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAtt                                                                                                   <  1:3420603/150‑1 (MQ=255)
                                                   gAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTAt                                                                                                 <  1:4472629/150‑1 (MQ=255)
                                                   gAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTAt                                                                                                 <  1:1265631/150‑1 (MQ=255)
                                                    aaGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATc                                                                                                <  2:185925/150‑1 (MQ=255)
                                                        tgtgAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCa                                                                                            >  1:3139740/1‑150 (MQ=255)
                                                           gAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTg                                                                                         >  2:660072/1‑150 (MQ=255)
                                                           gAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTg                                                                                         >  2:1372331/1‑150 (MQ=255)
                                                            aaaCATAATGTTAACTTCTTCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGc                                                                                        <  2:1776217/150‑1 (MQ=255)
                                                            aaaCATAATGTTAACGTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGc                                                                                        <  2:1774919/150‑1 (MQ=255)
                                                                aTAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATg                                                                                    >  2:1566672/1‑150 (MQ=255)
                                                                  aaTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCt                                                                                  >  1:4030031/1‑150 (MQ=255)
                                                                  aaTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCt                                                                                  <  2:4100034/150‑1 (MQ=255)
                                                                    tGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTc                                                                                <  1:693452/150‑1 (MQ=255)
                                                                      ttAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGc                                                                              >  1:2953277/1‑150 (MQ=255)
                                                                      ttAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGc                                                                              >  1:210863/1‑150 (MQ=255)
                                                                      ttAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGc                                                                              >  1:210868/1‑150 (MQ=255)
                                                                        aaCTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCaa                                                                            >  2:1836108/1‑150 (MQ=255)
                                                                         aCTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAat                                                                           >  1:2920144/1‑150 (MQ=255)
                                                                          cTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAata                                                                          <  2:126538/150‑1 (MQ=255)
                                                                          cTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAata                                                                          <  2:127106/150‑1 (MQ=255)
                                                                            tctcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATg                                                                        >  1:2610711/1‑150 (MQ=255)
                                                                              tcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgc                                                                      <  1:4328270/150‑1 (MQ=255)
                                                                                cATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgcgc                                                                    >  2:501816/1‑150 (MQ=255)
                                                                                 aTACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCa                                                                   >  2:3373155/1‑150 (MQ=255)
                                                                                 aTACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCa                                                                   >  2:3513936/1‑150 (MQ=255)
                                                                                      ttGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATg                                                              >  2:737856/1‑150 (MQ=255)
                                                                                      ttGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATg                                                              >  1:3399211/1‑150 (MQ=255)
                                                                                       tGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGa                                                             >  1:351021/1‑150 (MQ=255)
                                                                                        ggATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGGCACTGCAATGCGTCGCAATATGGCGCAAAATGAc                                                            >  1:1148363/1‑150 (MQ=255)
                                                                                         gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAcc                                                           >  2:4004244/1‑150 (MQ=255)
                                                                                          aTAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCa                                                          <  1:1463280/150‑1 (MQ=255)
                                                                                          aTAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCa                                                          <  1:4086910/150‑1 (MQ=255)
                                                                                              ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAg                                                      <  2:3633294/150‑1 (MQ=255)
                                                                                                aaaTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCg                                                    >  1:3483816/1‑150 (MQ=255)
                                                                                                aaaTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCg                                                    >  1:1584036/1‑150 (MQ=255)
                                                                                                  aTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGt                                                  >  2:3656938/1‑150 (MQ=255)
                                                                                                     cAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttga                                               <  2:4773726/150‑1 (MQ=255)
                                                                                                       gACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgatt                                             <  1:2501698/150‑1 (MQ=255)
                                                                                                       gACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgatt                                             <  1:107410/150‑1 (MQ=255)
                                                                                                         cATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattga                                           <  1:100038/150‑1 (MQ=255)
                                                                                                         cATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattga                                           >  2:1828015/1‑150 (MQ=255)
                                                                                                          aTGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTtgattgat                                          <  2:169751/150‑1 (MQ=255)
                                                                                                            gAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTTCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCa                                        <  1:2805532/150‑1 (MQ=255)
                                                                                                             aaaaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAg                                       >  1:1749921/1‑150 (MQ=255)
                                                                                                               aaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGt                                     >  2:4017977/1‑150 (MQ=255)
                                                                                                               aaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGt                                     <  2:3525232/150‑1 (MQ=255)
                                                                                                               aaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGt                                     <  2:3525949/150‑1 (MQ=255)
                                                                                                                    tcATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAgg                                >  2:2500928/1‑150 (MQ=255)
                                                                                                                         gCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGgcg                           <  2:657514/150‑1 (MQ=255)
                                                                                                                         gCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGgcg                           >  2:771744/1‑150 (MQ=255)
                                                                                                                           tGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCt                         <  2:3961068/150‑1 (MQ=255)
                                                                                                                              gttgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTa                      <  2:4217915/150‑1 (MQ=255)
                                                                                                                              gttgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTa                      <  1:2762347/150‑1 (MQ=255)
                                                                                                                               ttgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTAc                     >  1:937228/1‑150 (MQ=255)
                                                                                                                                tgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACg                    >  1:2646744/1‑150 (MQ=255)
                                                                                                                                 gttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGa                   <  1:1566672/150‑1 (MQ=255)
                                                                                                                                 gttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGa                   >  1:538617/1‑150 (MQ=255)
                                                                                                                                 gttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGa                   >  2:4681775/1‑150 (MQ=255)
                                                                                                                                      ttAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTaa              <  1:4505531/150‑1 (MQ=255)
                                                                                                                                        aaGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAg            >  1:802924/1‑150 (MQ=255)
                                                                                                                                         aGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGc           >  1:1201258/1‑150 (MQ=255)
                                                                                                                                          gCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGcc          <  1:764589/150‑1 (MQ=255)
                                                                                                                                           cTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGccc         <  1:2095851/150‑1 (MQ=255)
                                                                                                                                            ttGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCg        >  1:3259458/1‑150 (MQ=255)
                                                                                                                                            ttGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCg        >  2:3086565/1‑150 (MQ=255)
                                                                                                                                              gCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGAt      <  2:4299885/150‑1 (MQ=255)
                                                                                                                                                ccAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGc    >  2:2380191/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  >  CP009273/396736‑397031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑