Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 189,874 A→C 12.7% E172D (GAA→GAC)  frr → ribosome recycling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273189,8740AC12.7% 570.0 / 7.5 204E172D (GAA→GAC) frrribosome recycling factor
Reads supporting (aligned to +/- strand):  ref base A (98/73);  new base C (3/30);  total (101/103)
Fisher's exact test for biased strand distribution p-value = 1.86e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

AAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCAC  >  CP009273/189726‑190021
                                                                                                                                                    |                                                                                                                                                   
aaGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGaa                                                                                                                                                     >  2:28029/1‑149 (MQ=255)
aaGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGaa                                                                                                                                                     <  2:142434/149‑1 (MQ=255)
aaGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGaa                                                                                                                                                     >  2:119568/1‑149 (MQ=255)
 aGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAg                                                                                                                                                    >  1:1169709/1‑149 (MQ=255)
 aGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAg                                                                                                                                                    <  1:1004417/149‑1 (MQ=255)
 aGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAg                                                                                                                                                    >  1:3482185/1‑149 (MQ=255)
  gcgcGTGTTGCGGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCAGTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGGACAGAAACTGACTGATGCTGCACTCAAGAATCTTGACgc                                                                                                                                                   >  2:3408226/1‑149 (MQ=255)
  gcgcGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAgc                                                                                                                                                   >  1:962684/1‑149 (MQ=255)
  gcgcGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAgc                                                                                                                                                   >  2:1006780/1‑149 (MQ=255)
          tGCAGTACTTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTTTTGAAATATAAAGAGATCAGCGAAGACGACGATCGCCGTTATCAGGACGATGTACAGAAACTGACTTATGCTGCAATCAAGAAAATTGAAGCGGCGCTgg                                                                                                                                           <  2:669293/149‑1 (MQ=255)
           gCAGTACGTAACGTGCGTCGTGACGCTAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTTAAGCGGCGCTGGc                                                                                                                                          <  2:2184629/149‑1 (MQ=255)
           gCAGTACGTAACGTGCGTCGTGACGCTAACGAAAATGTTAAAGCACTGTTTAAATATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGAAATCAATAAATTTGAAGCGGCGCTGGc                                                                                                                                          <  2:3214824/149‑1 (MQ=255)
           gCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGACGCGGCGCTGGc                                                                                                                                          >  1:1244997/1‑149 (MQ=255)
                  gTAACGTGCTTCGTGACGCGAACGAAAAAGTGAAAGCACGGTTGAAATATAAAGAGATCAGCTAAGACGACGATCGCCGTTCTCAGGACGATGTACATAAACTGACTGATGCTGAAATCAAGAAAATTGAAGCGGCGCTGGCAGACaaa                                                                                                                                   <  2:183414/149‑1 (MQ=255)
                    aaCGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGTAGCGGCGCTGGCAGACAaaga                                                                                                                                 >  2:3962730/1‑149 (MQ=255)
                    aaCGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAaaga                                                                                                                                 >  1:775243/1‑149 (MQ=255)
                       gTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGc                                                                                                                              >  1:2304485/1‑149 (MQ=255)
                        tGCGTCGTGAGGCGAACTACAATGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTAAAGAAACTGACTGATGCTGCAATCAAGAAAATTGACGCGGCGCTGGCAGACAAAGAAGCa                                                                                                                             <  1:2260467/149‑1 (MQ=255)
                           gtggtGACGCTAACTAAAATGTGAAAGCACTTTTGAAAGATAAATAGATCCGCGATGACGACTATCGCCGTTCTCAGGACTTTGTACAGAAACTGACTGATGCTGAAATCAAGAAATTTGAAGCGGCGCTGGCAGACAAAGAAGCAGaa                                                                                                                          <  1:3726613/146‑1 (MQ=255)
                           gtcgtTACGCTAACGACAAATTGAAAGCACTGTTTAATGATAAAGAGATAAGCGAAGACGACGATCGCCGTTCTCAGGACTATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGaa                                                                                                                          <  2:3283478/149‑1 (MQ=255)
                           gtcgtGACGCGAACGACAAAGTTAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAATAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGaa                                                                                                                          <  2:3283940/149‑1 (MQ=255)
                                gACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGAt                                                                                                                     >  2:201862/1‑149 (MQ=255)
                                           cAAAGTGAAAGAACTGTTGAAAGATAAAGAGATCAGCGCAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGAt                                                                                                          <  2:3661951/149‑1 (MQ=255)
                                            aaatttaaaGCACTTTTTAAATATAAATAGAACAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTAAATAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGAtt                                                                                                         <  2:3115522/143‑1 (MQ=255)
                                            aaaGTGAAAGCACTTTTGAAATATAAAGATATCAGCGACGACGACTATCGCCGTTCTCAGGACTATTTACAGAAACTGACTGATGCTGCAAACAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGAtt                                                                                                         <  2:826372/149‑1 (MQ=255)
                                              aGTGAAAGCACTTTTGAAATATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTc                                                                                                       <  2:3976171/149‑1 (MQ=255)
                                              aGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTc                                                                                                       >  2:3614503/1‑149 (MQ=255)
                                                 gAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTg                                                                                                    >  2:3847768/1‑149 (MQ=255)
                                                   aaccACTGTTTAAATAAAAAGAGATCAGCGAACACGACGATCGCCGTACTCAGGACGTTGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGaa                                                                                                  <  2:2341096/146‑1 (MQ=255)
                                                   aaGCACTGTTTAAAGATAAAGAGATCAGCTAAGACGCCGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAATAAATTTGACGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGaa                                                                                                  <  2:1915621/149‑1 (MQ=255)
                                                    aGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAAc                                                                                                 <  2:4481506/149‑1 (MQ=255)
                                                    aGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAAc                                                                                                 <  2:1110553/149‑1 (MQ=255)
                                                     gCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACg                                                                                                >  1:508777/1‑149 (MQ=255)
                                                     gCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACg                                                                                                >  1:2290841/1‑149 (MQ=255)
                                                     gCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACg                                                                                                >  1:4108514/1‑149 (MQ=255)
                                                       aCTTTTGAAATAAAACGCTATCAGCGACGACGACGAGCGCCGTTCTCAGGACGATGAACAGAAACTGACTGCTGCTGCAATCAATAAAATTCACGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGAc                                                                                              <  1:121275/149‑1 (MQ=255)
                                                       aCTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGAc                                                                                              >  2:3825030/1‑149 (MQ=255)
                                                       aCTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGAc                                                                                              >  1:877877/1‑149 (MQ=255)
                                                         tGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGAGTTCTTGAACGACaa                                                                                            >  2:3493345/1‑149 (MQ=255)
                                                            ttaaaTATAACGAGATCAGCGAAGACGACGATCGCGGTTCTCAGGACTTTGTACAGAAACTGACTTATGCTGAAATCAAGAAAATTGACGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACaaaaa                                                                                         <  1:2515555/147‑1 (MQ=255)
                                                            tGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACaaaaa                                                                                         <  2:727015/149‑1 (MQ=255)
                                                             gAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAc                                                                                        >  2:3758091/1‑149 (MQ=255)
                                                                aGATAAAGATATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAACAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAcgcc                                                                                     <  2:4149613/149‑1 (MQ=255)
                                                                   tAAAGAGATCCGCTAAGACGACTATCGCGTTTATACGGACTTTTTACTGAAACTGACTTATGCTGAAATAAAGAAATTTGAAGCGGCGCTGGCAGACAAAGACGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCt                                                                                  <  2:2133728/149‑1 (MQ=255)
                                                                   tAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCt                                                                                  >  1:588547/1‑149 (MQ=255)
                                                                    aaaGAGATCAGCTAAGACGACGATCGCGGTTATCAGGACGTTGTAATTAAACTGACTTATGCTGAAATCAATAAAATTGAAGCGGCGCTGGAATACAAAGAAGCAGAACTGATGCTGTTCTGATTTATTTAACGACAAAACCGCCGCTc                                                                                 <  2:3354431/149‑1 (MQ=255)
                                                                      agagATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAg                                                                               >  1:884927/1‑149 (MQ=255)
                                                                      agagATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAg                                                                               >  1:4275345/1‑149 (MQ=255)
                                                                       gagaTCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGt                                                                              >  2:1331274/1‑149 (MQ=255)
                                                                          aTCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGa                                                                           >  2:1367884/1‑149 (MQ=255)
                                                                            cAGCTAAGACGACTATCGCGGTTCTCTGTACGTTTAACAGAAACTGAGTTTTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATc                                                                         <  2:4347867/149‑1 (MQ=255)
                                                                             aGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAATTTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATcc                                                                        <  2:2653016/149‑1 (MQ=255)
                                                                             aGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATcc                                                                        >  2:732552/1‑149 (MQ=255)
                                                                                 aaGCCGCCGAGCGCGGTTCTCTGGACTTTGAACAGAATCTCTCTGCTGCTGCAACCATTAAATTTGCCGCGGCGCTGGCAGACAACGACGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAACACGCCGCTCAGTAGATCCTTGc                                                                    <  2:3099753/149‑1 (MQ=255)
                                                                                 aaGACGACGATCGCCGTTCTCAGGACGATGTACAGAAAGTGACTGATGCTGAAATAAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGc                                                                    <  2:3544844/149‑1 (MQ=255)
                                                                                   gacgacGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTCGATCCTTGCgg                                                                  >  2:2584319/1‑149 (MQ=255)
                                                                                     cgacgaTCGCCGTTCTCAGGACGATGTACATAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGAt                                                                <  2:2490395/149‑1 (MQ=255)
                                                                                      gacgaGCGCCGTTCTCAGGACTATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATc                                                               <  2:2142703/149‑1 (MQ=255)
                                                                                       acgaTCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCg                                                              >  2:1513123/1‑149 (MQ=255)
                                                                                         gaTCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGc                                                            >  2:4571287/1‑149 (MQ=255)
                                                                                          aTCGCCGTTCTCAGGACGATGTACATAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCt                                                           <  2:2209054/149‑1 (MQ=255)
                                                                                          aTCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCt                                                           >  2:3343392/1‑149 (MQ=255)
                                                                                          aTCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCt                                                           >  2:1795720/1‑149 (MQ=255)
                                                                                          aTCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCt                                                           <  2:4050723/149‑1 (MQ=255)
                                                                                              ccGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTggcg                                                       >  1:1915969/1‑149 (MQ=255)
                                                                                                 ttCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGgcggcg                                                    >  2:3456066/1‑149 (MQ=255)
                                                                                                  tctcCGGACTATGAACAGAAACTGACTTAGGCTGAAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGt                                                   <  2:2015710/149‑1 (MQ=255)
                                                                                                      aGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTGTTg                                               >  2:1545908/1‑149 (MQ=255)
                                                                                                       ggACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTgg                                              >  1:2403907/1‑148 (MQ=255)
                                                                                                        gACGATGTACAGAAACTGACTGATGCTGAAATCAAGAAAATTTAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCAGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCt                                             <  2:1287658/149‑1 (MQ=255)
                                                                                                          ctttGTAAAAAAACTGAGTTATGCTCAAAACAACAAAATTCACGCGGCGCTGGCAGACAACCAAGCCGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCttt                                           <  1:3666182/146‑1 (MQ=255)
                                                                                                           gATGTACAGAAACTGACTGAGGCTGAAATCAATAAAATTGACGCGGCGCGGGCAGACAAAGACGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCtttt                                          <  1:2559223/149‑1 (MQ=255)
                                                                                                              taaccgAAACTGACTGAGGCTGCAATAAATAAAATTGACGCGGCGCGGGCAGACACAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGCCAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTAt                                       <  2:3068166/144‑1 (MQ=255)
                                                                                                                aCATAAACTGACTTATGCTCAAAAAAATAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTc                                     <  2:863315/149‑1 (MQ=255)
                                                                                                                 cataaaCTTAGTTATGCTGAAAACAAGAAATTTTAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCt                                    <  1:1411534/146‑1 (MQ=255)
                                                                                                                  aGACACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTg                                   <  2:3811304/149‑1 (MQ=255)
                                                                                                                    aaaCTGACTTATGCGCCAATCAACAAAATTGAAGCGGCGCGGGCAGACAAAGAAGCAGACCTGATGCATTTCTGATTTCTTGAACGACAAACACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGtc                                 <  1:2932627/149‑1 (MQ=255)
                                                                                                                    aaaCTGACTGATGCTGCAATCAATAAAATTGACGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGtc                                 <  2:3706140/149‑1 (MQ=255)
                                                                                                                     aaCTGCCTGCTGCTCCAATCAACAAAATTGCCGCGGCGCTGGCAGACAACGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGtct                                <  1:2779538/149‑1 (MQ=255)
                                                                                                                            tgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTGTGTCTGCActct                         >  1:3136120/1‑149 (MQ=255)
                                                                                                                            tgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTGTTGGTTTTTTTTATGTGTCaaaact                         >  1:3568653/1‑145 (MQ=255)
                                                                                                                             gaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCGGTCTCAACTCTg                        >  2:764769/1‑149 (MQ=255)
                                                                                                                                  tgcAAGCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGACCTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGt                   <  2:4377976/149‑1 (MQ=255)
                                                                                                                                  tgaaaTCAACAAAATTTACGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGt                   <  1:1823338/146‑1 (MQ=255)
                                                                                                                                   gcAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTGTTGCTTTTTATTATGTCTCAACACGGGATGtt                  >  1:239194/1‑149 (MQ=255)
                                                                                                                                    aaaTCAATAAATTGTACGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGttt                 <  1:120125/148‑1 (MQ=255)
                                                                                                                                     aaTCACGAAATTTGAAGCGGCGCTGGCAGACAAAGACGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTc                <  2:4251072/149‑1 (MQ=255)
                                                                                                                                     aaTCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTc                <  2:164779/149‑1 (MQ=255)
                                                                                                                                       tCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTCTTCTGTCGCAACGCTGGCTGTTTCAt              >  2:417567/1‑149 (MQ=255)
                                                                                                                                       tCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCAt              <  2:337311/149‑1 (MQ=255)
                                                                                                                                        cAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTTCTTTTTTTTCTGTCTCAACACTGGATGTTTCATg             >  1:2842993/1‑149 (MQ=255)
                                                                                                                                          aGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTGTTTGTTTTTTTTATTTATCAACAATGGTTTTTTGATGaa           >  1:2369664/1‑149 (MQ=255)
                                                                                                                                           taaaaTTGAAGCGGCGCTGGCAGACAAAGACGCAGACCTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAg          <  2:87003/148‑1 (MQ=255)
                                                                                                                                             aaaTTTACGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCTGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCa        <  1:4415724/149‑1 (MQ=255)
                                                                                                                                              aaTTGACGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCaa       <  1:4566738/149‑1 (MQ=255)
                                                                                                                                               ttggAAGCGGCGCTGGCAGACAAAGAACCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAAc      <  1:3972398/146‑1 (MQ=255)
                                                                                                                                                  gAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAACACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCa   <  2:552754/149‑1 (MQ=255)
                                                                                                                                                  gAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAAcaca   >  2:1116861/1‑149 (MQ=255)
                                                                                                                                                   aaGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGCAGCAACTCAc  >  2:1291147/1‑149 (MQ=255)
                                                                                                                                                   aaGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCAc  <  2:2857505/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCAC  >  CP009273/189726‑190021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑