Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,333 A→T 9.4% intergenic (+88/‑256) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,3330AT 9.4% 674.7 / 5.8 225intergenic (+88/‑256)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base A (64/128);  new base T (13/3);  total (94/131)
Fisher's exact test for biased strand distribution p-value = 2.31e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGT  >  CP009273/450185‑450481
                                                                                                                                                    |                                                                                                                                                        
aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGa                                                                                                                                                          <  1:3939713/149‑1 (MQ=255)
 gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATTTTTCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAt                                                                                                                                                         <  2:266377/149‑1 (MQ=255)
   gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGAttt                                                                                                                                                       >  2:3107716/1‑149 (MQ=255)
     tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑g                                                                                                                                                    <  2:3003695/149‑1 (MQ=255)
     tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑g                                                                                                                                                    >  2:716717/1‑149 (MQ=255)
      gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGAtttttc                                                                                                                                                    >  2:1620374/1‑147 (MQ=255)
        aGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑gcgc                                                                                                                                                 >  2:1446885/1‑149 (MQ=255)
        aGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTGTGTGGGtttttggg                                                                                                                                                  >  1:480177/1‑145 (MQ=255)
             acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑ttttt                                                                                                                                           <  2:3641652/149‑1 (MQ=255)
              ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGATTTT‑GAGC‑tttttt                                                                                                                                          >  1:1792276/1‑148 (MQ=255)
                 tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGGTTTT‑TGGC‑ttttttacc                                                                                                                                       >  2:2784280/1‑145 (MQ=255)
                 tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGAGTTT‑GCGG‑ttttttgcc                                                                                                                                       >  2:2779092/1‑145 (MQ=255)
                   tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGCTTTT‑GGGC‑ttttttgcccg                                                                                                                                     >  1:4506087/1‑143 (MQ=255)
                   tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGGTTTTT‑TCGC‑TTTTTTGCCAg                                                                                                                                     >  1:2658924/1‑149 (MQ=255)
                     tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTTGGTGGATTTT‑TGGC‑TTTTTTCGCAGcc                                                                                                                                   >  2:789350/1‑148 (MQ=255)
                         tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGGTTTGTTTTTTGTGGGTTTT‑TCGG‑TTTTTTGCCAGCAttaa                                                                                                                               >  1:2891179/1‑147 (MQ=255)
                           tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑a                                                                                                                            <  1:2171705/149‑1 (MQ=255)
                            cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTTGTTTTT‑GGGC‑GTTTTTGCCAGCATTAAG‑Ac                                                                                                                           >  1:4151321/1‑149 (MQ=255)
                               cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑aa                                                                                                                       >  2:4435479/1‑149 (MQ=255)
                                 cTGTCGTGGTGCAGGAAGCTTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCTGTATGTTGCTAATGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑aaaa                                                                                                                     <  1:3717773/149‑1 (MQ=255)
                                  tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAAt                                                                                                                    <  1:3228492/149‑1 (MQ=255)
                                  tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAAt                                                                                                                    <  1:3300607/149‑1 (MQ=255)
                                  tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAAt                                                                                                                    <  1:1001314/149‑1 (MQ=255)
                                  tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAAt                                                                                                                    <  2:3995458/149‑1 (MQ=255)
                                    tCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGGATTTT‑GGGC‑TTTTTGGCCAGCAATCAG‑ACG‑AAAATTg                                                                                                                  >  2:1975016/1‑149 (MQ=255)
                                        ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCg                                                                                                              <  2:3735854/149‑1 (MQ=255)
                                            cAGGAAGCATCGCGTAAACACGCATTTGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGaa                                                                                                          <  2:2333834/149‑1 (MQ=255)
                                             aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAAt                                                                                                         <  2:29420/149‑1 (MQ=255)
                                             aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTTGTTTTT‑GAGC‑TTTTTGCCCACCATTAAG‑ACG‑AAAATTTCCCGGGAAt                                                                                                         >  2:531856/1‑149 (MQ=255)
                                             aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGGGGTGTTTTTTTTGGGTTTT‑GGGG‑TTTTTTGCCCCCAATCAG‑AAGAAAAATTGCCCgggct                                                                                                          >  1:1705260/1‑147 (MQ=255)
                                                  gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGATTTT‑TGGC‑TTTTTTGCCAGCATTAAG‑ACG‑AAACTTTGCCGGGGATtgtgt                                                                                                    >  2:2375588/1‑148 (MQ=255)
                                                  gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGa                                                                                                    <  1:897688/149‑1 (MQ=255)
                                                       gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTTTGGTTTTT‑TGGG‑TTTTTTGCCAGCCTTAAG‑ACG‑AACATTGGGCGGGGATTGTGtgaaaa                                                                                               >  2:416170/1‑149 (MQ=255)
                                                        cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAAt                                                                                              <  1:1697880/149‑1 (MQ=255)
                                                          tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATAc                                                                                            <  2:2286636/149‑1 (MQ=255)
                                                          tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAAGTTCCCGGGAATTGTGAAAAAATaa                                                                                            >  2:2142473/1‑148 (MQ=255)
                                                           aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTT‑GCGC‑TTTTTTCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATAcg                                                                                           >  2:4459818/1‑149 (MQ=255)
                                                            aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgc                                                                                          <  2:2867689/149‑1 (MQ=255)
                                                               aCGCATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGAc                                                                                       <  2:1996692/149‑1 (MQ=255)
                                                               aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGGTTTGTTTTTTATGGTTTTT‑TGGCGTTTTTGCCC‑GCATTAAG‑ACC‑AAAATTTGCCGGGGATTGTGTAAAAATAagcggc                                                                                       >  2:1533084/1‑147 (MQ=255)
                                                               aCGCATTTGCAACTGTCGGCGCATTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGAc                                                                                       <  2:319997/149‑1 (MQ=255)
                                                                 gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGGTTTTGGGGG‑TTTTTGCCC‑GCCATGAG‑ACG‑AAAAATGCCCCGGACTTTTGTAAAAATAAGCGACAg                                                                                     >  1:1845003/1‑149 (MQ=255)
                                                                 gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGGTTTT‑TGGG‑GTTTTTACCAGCCTTCAG‑ACG‑AAAAATGCCCGGGAAATGTGAAAAAATAAACGACac                                                                                     >  2:4338891/1‑148 (MQ=255)
                                                                            tgtgGGCGCTTTTCCAGTTTGTTGATAAATTTTTTTTTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                                                                          <  1:82882/149‑1 (MQ=255)
                                                                            tGTCGGCGCTTTACAAGTATTTTTCAAAATATTTTATTAAAAACGGCCTGCGGGCCTTTTTGTTTTGTCTGTATTTT‑GCGC‑TTTTTGCCCAGAATTCAG‑ACT‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                                                                          <  1:4274645/149‑1 (MQ=255)
                                                                                 gcgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtc                                                                     <  2:2391191/149‑1 (MQ=255)
                                                                                  cgcTTTTCCTTTATGTTGCTAATTTTTTTATTAAAAACGGCCTGCGGGCCTTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                                                                    <  1:1212446/149‑1 (MQ=255)
                                                                                    cTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                                  <  2:1064262/149‑1 (MQ=255)
                                                                                     ttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑TCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATAAGCGTCAGCGCGCAAAAACCGTTCTCGa                                                                 >  2:3029488/1‑149 (MQ=255)
                                                                                      tttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                                                                <  2:2836149/149‑1 (MQ=255)
                                                                                      cttCAAGAATGTTGCTAAAGATTTTTTGACAAACGGCCTGCGGGCCGTTCTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATCCAG‑ACG‑AAAATTGCCCGGGCATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                                                                <  1:2978227/148‑1 (MQ=255)
                                                                                        tCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc                                                              <  2:142222/149‑1 (MQ=255)
                                                                                        tCCAGTATGTTGATAAATATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc                                                              <  1:342558/149‑1 (MQ=255)
                                                                                           aGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGGATTTT‑TCGC‑ATTTTTCCCACCAAACAGAACA‑AAAATGGCCCGGAAATT‑TGAAAAAATAATCGACACCGCGCAAAAACCCTTCGCGAcaaaat                                                           >  1:4141825/1‑144 (MQ=255)
                                                                                                gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                      <  1:3962554/149‑1 (MQ=255)
                                                                                                gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCAATCAG‑ACG‑AAAATTGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAAACGTTCTCGACTCATAAAAGt                                                      >  2:2980949/1‑149 (MQ=255)
                                                                                                  ggCTAATGATTTTTTGAAAAAGGGCCTGCGGGCCGTTTTGTTTTGTCTGGTTTTT‑GCGC‑TTTTGGCCCAGCATTCAG‑ACG‑AAATTTGCCCGGGATTGGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa                                                    <  1:1621819/148‑1 (MQ=255)
                                                                                                    cTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTT‑TCGC‑TTTTTTCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTTAAAAAAAAAGCGACACCGCGCAATAACCGTTATCGACACAAAAAAGTTATg                                                  >  2:4028203/1‑149 (MQ=255)
                                                                                                    cTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑TCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg                                                  >  2:763719/1‑149 (MQ=255)
                                                                                                     taaatttttaATTAAAAACGGCCTGGGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                 <  1:1161863/143‑1 (MQ=255)
                                                                                                        aGATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTATGGATTTT‑TCGC‑TTTTTGCCCAGCATTAAG‑AAG‑AAAAATGCCCGGGTATTGTGAAAAAATAAACGCCACCGCGCAAAAAACCTTATCGACAACTAAAATTGATTCCGc                                              >  2:623273/1‑149 (MQ=255)
                                                                                                         gATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCt                                             <  1:1051054/149‑1 (MQ=255)
                                                                                                          aTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGTTTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGCATTGTGAAAAAATACGCGACAGCGCGCAATAACCCTTCTCGACTCCTAAAAGTGATGCCGcga                                            >  2:3410862/1‑147 (MQ=255)
                                                                                                          aTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                                            <  2:2633101/149‑1 (MQ=255)
                                                                                                          aTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                                            <  1:3796971/149‑1 (MQ=255)
                                                                                                             ttATCAAAAACGGCCTGCGGGCCGTTTTTTTTTGTCTGGTTTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATaa                                         <  1:2428604/149‑1 (MQ=255)
                                                                                                               aTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATg                                       <  2:82147/149‑1 (MQ=255)
                                                                                                                tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGc                                      <  1:1055365/149‑1 (MQ=255)
                                                                                                                 gAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGCTTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTTCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAAACGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                                     >  2:1421000/1‑149 (MQ=255)
                                                                                                                 gAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                                     <  2:1057861/149‑1 (MQ=255)
                                                                                                                   aaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc                                   <  1:1489417/149‑1 (MQ=255)
                                                                                                                          ccTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAt                            <  2:495615/149‑1 (MQ=255)
                                                                                                                           cTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAtt                           <  1:2761867/149‑1 (MQ=255)
                                                                                                                           cTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TATTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAtt                           <  1:3677432/149‑1 (MQ=255)
                                                                                                                           cTGCGGGCCGTGTTGTTTTTTCTGGATTTT‑TCGC‑GTTTTTACCAGCCTTCAG‑ACC‑AAAAATGCCCCGGAAATGTGAAAAAATACGCGACACCGCCCAATAACCCGTCTCGACTCATAAAAGTGATGCCGCTAAAATGCCGCCTccactt                           >  2:2452941/1‑145 (MQ=255)
                                                                                                                            tGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt                          <  2:4563840/149‑1 (MQ=255)
                                                                                                                             gCGGGCCTTTTTGTTTTGTGTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACT‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTg                         <  1:3487724/149‑1 (MQ=255)
                                                                                                                              cGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGa                        <  1:3180690/149‑1 (MQ=255)
                                                                                                                               gggCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa                       <  2:2259227/149‑1 (MQ=255)
                                                                                                                                   cGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCt                   >  1:2276140/1‑149 (MQ=255)
                                                                                                                                    gttttgttttGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                  <  2:748116/149‑1 (MQ=255)
                                                                                                                                     ttttgttttgTCTGGATTTT‑GCGA‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt                 <  1:4338986/149‑1 (MQ=255)
                                                                                                                                      tttgttttgtCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                >  1:4446685/1‑149 (MQ=255)
                                                                                                                                         gttttgtCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCggg             >  1:3840661/1‑149 (MQ=255)
                                                                                                                                          ttttgtCTGTATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga            <  1:1922617/149‑1 (MQ=255)
                                                                                                                                          ttttgtCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga            <  1:126407/149‑1 (MQ=255)
                                                                                                                                          ttttgtCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga            <  2:3534747/149‑1 (MQ=255)
                                                                                                                                          ttttgtCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga            <  2:2556294/149‑1 (MQ=255)
                                                                                                                                           tttgtCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat           <  2:3285675/149‑1 (MQ=255)
                                                                                                                                               tCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt       >  2:3688388/1‑149 (MQ=255)
                                                                                                                                               tCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt       >  2:1508671/1‑149 (MQ=255)
                                                                                                                                               tCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt       >  1:120172/1‑149 (MQ=255)
                                                                                                                                               tCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt       >  2:2063731/1‑149 (MQ=255)
                                                                                                                                                cTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTc      <  2:1257284/149‑1 (MQ=255)
                                                                                                                                                cTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTc      <  2:2777358/149‑1 (MQ=255)
                                                                                                                                                 tGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt     <  1:1689212/149‑1 (MQ=255)
                                                                                                                                                 tGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt     >  1:1489842/1‑149 (MQ=255)
                                                                                                                                                  ggATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg    >  1:3261428/1‑149 (MQ=255)
                                                                                                                                                   gATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTgg   <  2:1204753/149‑1 (MQ=255)
                                                                                                                                                    aTTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGt  >  1:3815873/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                        
AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT‑GCGC‑TTTTTGCCCAGCATTCAG‑ACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGT  >  CP009273/450185‑450481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑