Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,795,380 A→T 68.4% V483E (GTA→GAA)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,795,3800AT68.4% 382.8 / 274.1 228V483E (GTA→GAA) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (36/36);  new base T (83/73);  total (119/109)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 239 total aligned reads displayed.

AACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCC  >  CP009273/1795234‑1795527
                                                                                                                                                  |                                                                                                                                                   
aaCCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgttct                                                                                                                                                   >  1:4055782/1‑149 (MQ=255)
   cAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAATGAGAAGTCCAGctgtactgta                                                                                                                                                >  2:833136/1‑149 (MQ=255)
   cAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgttctgta                                                                                                                                                <  2:4447290/149‑1 (MQ=255)
    aGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGACGTCCAGCtgttctgtac                                                                                                                                               >  1:2693209/1‑149 (MQ=255)
    aGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCtgtactgtac                                                                                                                                               >  2:2698583/1‑149 (MQ=255)
         aCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCAc                                                                                                                                          >  1:4277649/1‑149 (MQ=255)
           tctTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTg                                                                                                                                        >  2:1773184/1‑149 (MQ=255)
           tctTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTg                                                                                                                                        >  2:1740328/1‑149 (MQ=255)
             ttCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTAGATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGcc                                                                                                                                      <  2:2945315/149‑1 (MQ=255)
                  tCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCa                                                                                                                                 <  1:3209619/149‑1 (MQ=255)
                     ggATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGa                                                                                                                              >  2:2164680/1‑149 (MQ=255)
                          ccGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAg                                                                                                                         >  1:3155614/1‑149 (MQ=255)
                            gATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGc                                                                                                                       <  1:4106312/149‑1 (MQ=255)
                            gATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGc                                                                                                                       >  1:3623920/1‑149 (MQ=255)
                            gATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGc                                                                                                                       >  1:1313255/1‑149 (MQ=255)
                              tGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAg                                                                                                                     >  2:3199714/1‑149 (MQ=255)
                              tGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAg                                                                                                                     >  2:3199753/1‑149 (MQ=255)
                               gAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGt                                                                                                                    <  1:3245877/149‑1 (MQ=255)
                                 aaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCa                                                                                                                  >  1:2344557/1‑149 (MQ=255)
                                  aCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCAt                                                                                                                 >  2:2604942/1‑149 (MQ=255)
                                   cGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATCAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATa                                                                                                                >  1:2615200/1‑149 (MQ=255)
                                    gTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTGACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATAc                                                                                                               >  1:187171/1‑149 (MQ=255)
                                       ccATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAgg                                                                                                            >  2:2564279/1‑149 (MQ=255)
                                       ccATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAgg                                                                                                            >  2:75012/1‑149 (MQ=255)
                                        cATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAggg                                                                                                           <  1:2175063/149‑1 (MQ=255)
                                            gACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTaaa                                                                                                       <  2:2243872/149‑1 (MQ=255)
                                              ccccAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAAtt                                                                                                     >  1:523694/1‑149 (MQ=255)
                                              ccccAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAAtt                                                                                                     <  1:3939699/149‑1 (MQ=255)
                                                  aGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAAt                                                                                                 <  1:3492638/149‑1 (MQ=255)
                                                  aGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAAt                                                                                                 <  1:4504579/149‑1 (MQ=255)
                                                   gAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAAtt                                                                                                <  1:3166036/149‑1 (MQ=255)
                                                      ttGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTc                                                                                             <  1:2605344/149‑1 (MQ=255)
                                                      ttGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTc                                                                                             >  1:1883828/1‑149 (MQ=255)
                                                      ttGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCGACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCCTGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTc                                                                                             >  2:2517118/1‑149 (MQ=255)
                                                       tGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCg                                                                                            <  2:884522/149‑1 (MQ=255)
                                                         cgcgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGa                                                                                          <  2:2345680/149‑1 (MQ=255)
                                                          gcgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGAc                                                                                         <  1:4429906/149‑1 (MQ=255)
                                                          gcgGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGAc                                                                                         <  2:3516333/149‑1 (MQ=255)
                                                                aaTCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGa                                                                                   >  2:2589523/1‑149 (MQ=255)
                                                                aaTCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGa                                                                                   >  2:3324468/1‑149 (MQ=255)
                                                                  tCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGaaa                                                                                 <  1:77946/149‑1 (MQ=255)
                                                                    aTTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgc                                                                               >  2:214575/1‑149 (MQ=255)
                                                                    aTTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgc                                                                               <  2:636877/149‑1 (MQ=255)
                                                                    aTTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgc                                                                               >  1:1621572/1‑149 (MQ=255)
                                                                     gtACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAgcg                                                                              >  1:3680881/2‑149 (MQ=255)
                                                                       aCCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCATGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGcc                                                                            <  1:1169077/149‑1 (MQ=255)
                                                                       aCCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGcc                                                                            <  1:3313790/149‑1 (MQ=255)
                                                                       aCCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGcc                                                                            <  2:4515414/149‑1 (MQ=255)
                                                                         cGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCtt                                                                          <  1:1850494/149‑1 (MQ=255)
                                                                          ggTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTc                                                                         >  2:3652029/1‑149 (MQ=255)
                                                                           gTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCa                                                                        >  1:2674244/1‑149 (MQ=255)
                                                                             aCTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCAcc                                                                      <  2:2039377/149‑1 (MQ=255)
                                                                             aCTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCAcc                                                                      <  2:1582943/149‑1 (MQ=255)
                                                                              cTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCAccc                                                                     <  2:905369/149‑1 (MQ=255)
                                                                               tttACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCa                                                                    >  1:25257/1‑149 (MQ=255)
                                                                                ttACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAg                                                                   <  1:4375531/149‑1 (MQ=255)
                                                                                 tACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGt                                                                  >  2:121464/1‑149 (MQ=255)
                                                                                  aCGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTCGAAAGCGCCTTCACCCAGtt                                                                 >  2:3142871/1‑149 (MQ=255)
                                                                                  aCGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGtt                                                                 >  2:3142960/1‑149 (MQ=255)
                                                                                    gTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGa                                                               >  1:953312/1‑149 (MQ=255)
                                                                                    gTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGa                                                               >  1:514069/1‑149 (MQ=255)
                                                                                     ttCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGAAGAAAGCGCCTTCACCCAGTTGat                                                              >  2:427328/1‑149 (MQ=255)
                                                                                     ttCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGat                                                              >  1:2835738/1‑149 (MQ=255)
                                                                                      tCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGata                                                             <  2:3623737/149‑1 (MQ=255)
                                                                                       cATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGatat                                                            >  2:3733045/1‑149 (MQ=255)
                                                                                       cATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGatat                                                            <  2:3795268/149‑1 (MQ=255)
                                                                                       cATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGatat                                                            >  2:413856/1‑149 (MQ=255)
                                                                                        aTTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATAtt                                                           >  1:2335236/1‑149 (MQ=255)
                                                                                            tCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCaaa                                                       >  2:1004926/1‑149 (MQ=255)
                                                                                             cTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAAc                                                      <  1:4310979/149‑1 (MQ=255)
                                                                                             cTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAAc                                                      <  1:4310986/149‑1 (MQ=255)
                                                                                             cTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAAc                                                      <  2:1005966/149‑1 (MQ=255)
                                                                                                cGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACggg                                                   >  2:3682958/1‑149 (MQ=255)
                                                                                                cGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACggg                                                   <  2:2489373/149‑1 (MQ=255)
                                                                                                 gCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGa                                                  <  1:2396172/149‑1 (MQ=255)
                                                                                                   cTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtg                                                >  2:2948936/1‑149 (MQ=255)
                                                                                                      cATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGAtgttg                                             >  2:2812713/1‑149 (MQ=255)
                                                                                                         aagaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGttt                                          >  1:198174/1‑149 (MQ=255)
                                                                                                          agaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGtttt                                         >  2:11182/1‑149 (MQ=255)
                                                                                                           gaagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttc                                        <  2:1638612/149‑1 (MQ=255)
                                                                                                            aagCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTttct                                       <  1:2417177/149‑1 (MQ=255)
                                                                                                               cgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTcc                                    <  1:1539901/149‑1 (MQ=255)
                                                                                                               cgcTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTcc                                    <  1:671543/149‑1 (MQ=255)
                                                                                                                   cAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAgcg                                >  2:1278034/1‑149 (MQ=255)
                                                                                                                   cAGACGAGACGGCAAAGAGAAGTCAAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGAGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCAAgcg                                >  2:4362436/1‑149 (MQ=255)
                                                                                                                     gacgagacgGCAAAGAGAAGTCCATCTGTTCTGTACCGCACTGACATGAACGATCGAGGCAGACATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCa                              >  1:818121/1‑149 (MQ=255)
                                                                                                                       cgagacgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAAc                            <  1:2960549/149‑1 (MQ=255)
                                                                                                                        gagacgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAAcc                           >  1:3807984/1‑149 (MQ=255)
                                                                                                                         agacgGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCg                          >  1:1339262/1‑149 (MQ=255)
                                                                                                                                aaaGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTc                   >  1:1609750/1‑149 (MQ=255)
                                                                                                                                    agaAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCATTTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGcc               <  1:3114812/149‑1 (MQ=255)
                                                                                                                                    agaAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGcc               <  1:830191/149‑1 (MQ=255)
                                                                                                                                    agaAGTCCAGCTGGACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGcc               <  2:3147155/149‑1 (MQ=255)
                                                                                                                                     gaAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCt              <  2:3465486/149‑1 (MQ=255)
                                                                                                                                     gaAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCt              <  2:1688466/149‑1 (MQ=255)
                                                                                                                                     gaAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCt              >  1:696295/1‑149 (MQ=255)
                                                                                                                                     gaAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCt              <  2:1833832/149‑1 (MQ=255)
                                                                                                                                        gtacatctgttctgtaCCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATACAAATTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAg           >  1:3065807/7‑149 (MQ=255)
                                                                                                                                              ctgttctgtaCCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGAt     <  2:2447082/149‑1 (MQ=255)
                                                                                                                                                 tactgtacCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGCTGTTGTTTTCTTCCAGCGCAACCGCCCGGTCCGCCTCAGCACGATccc  >  2:1928791/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCATACAGGGTAAATTCAATTTTCGGACCGTAGAAAGCGCCTTCACCCAGTTGATATTCAAACGGGATGTTGTTTTCTTCCAGCGCAACCGCCAGGTCCGCCTCAGCACGATCCC  >  CP009273/1795234‑1795527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑