Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,823,835 A→C 7.6% W53G (TGG→GGG)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,823,8350AC 7.6% 719.0 / 4.4 272W53G (TGG→GGG) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (162/78);  new base C (3/27);  total (165/107)
Fisher's exact test for biased strand distribution p-value = 1.10e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAT  >  CP009273/1823688‑1823983
                                                                                                                                                   |                                                                                                                                                    
tcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAg                                                                                                                                                     >  1:3045837/1‑149 (MQ=255)
tcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGGTGCTCTGGCACTCATCGCGGGGCATTCGGCTTTAGTGCTTTCCAGCAGTGCGGCACAGCGTTCGACAACGGCATGACGTTCAGCAAGTGCTAGCCGCGCCCAg                                                                                                                                                     >  2:1577755/1‑149 (MQ=255)
 catcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgc                                                                                                                                                    <  1:448880/149‑1 (MQ=255)
   tcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAgcgc                                                                                                                                                  >  1:223642/1‑149 (MQ=255)
     gCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCgg                                                                                                                                                >  1:4178547/1‑149 (MQ=255)
      ccGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGa                                                                                                                                               >  1:2857641/1‑149 (MQ=255)
       cGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaa                                                                                                                                              >  2:1949293/1‑149 (MQ=255)
        gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaaa                                                                                                                                             >  1:1902099/1‑149 (MQ=255)
        gTCACTGCGGTGGCCGCTTCCCAGCGCGGCGTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACCGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGaaa                                                                                                                                             <  1:2005840/149‑1 (MQ=255)
         tCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                            >  2:2461864/1‑149 (MQ=255)
          cACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcg                                                                                                                                           >  2:1994038/1‑149 (MQ=255)
           aCTTGGGTTGCCGCTTCCCAGCGGGGATTACCCGTTTATCTGGCATTAATCGGGTTTATTTCGTATTTATTGCTTTCCAGCTGTGCGGCAAAGCGTTCGACAACGGAATTACGTTAAGAAAATGAAAGCCGCGCCCAGCGCGGAAAcgc                                                                                                                                          <  1:182830/149‑1 (MQ=255)
             ttCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgccg                                                                                                                                        >  2:4557539/1‑149 (MQ=255)
             ttCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAAcgccg                                                                                                                                        >  2:4557469/1‑149 (MQ=255)
                ggTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCAc                                                                                                                                     >  1:4095826/1‑149 (MQ=255)
                        cTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGcc                                                                                                                             >  2:3966574/1‑149 (MQ=255)
                              aGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACaa                                                                                                                       >  1:4144137/1‑149 (MQ=255)
                                 gcgGCTTACCCTTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGATTTCCAGCAGTGCGGCAAAGCGTTCGACACCGGCATGACGTTCAGCAAATCAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGcc                                                                                                                    <  2:4022629/149‑1 (MQ=255)
                                    gCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTACCCGACAAGCCTGc                                                                                                                 >  2:2063632/1‑149 (MQ=255)
                                    gCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                                                                 >  1:52952/1‑149 (MQ=255)
                                    gCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                                                                 >  1:3720093/1‑149 (MQ=255)
                                    gCTTACCCGTTTCTCGGGCAATAATCGGGTTTAATTCGGATTTATTGCTTTCCAGCTGTGCGGCAAAGCGTTCGACAAGGGCATGACGTTCAGCAAATTAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                                                                 <  2:223642/149‑1 (MQ=255)
                                      ttACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTc                                                                                                               >  1:57212/1‑149 (MQ=255)
                                       tACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCg                                                                                                              >  1:4091323/1‑149 (MQ=255)
                                            gTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCTGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTg                                                                                                         <  2:3777639/149‑1 (MQ=255)
                                            gTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTg                                                                                                         >  2:1489243/1‑149 (MQ=255)
                                                   tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggca                                                                                                  >  2:1660215/1‑149 (MQ=255)
                                                    ggCAATAAGCGGGGTTATTTCGGCTTTATGGCTTTCCAGCAGTGCGGAAATGCGTTCGACACCGGCATGACGTTAAGAAAATTAAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcat                                                                                                 <  2:940885/149‑1 (MQ=255)
                                                     gCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACACCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatc                                                                                                <  2:3896276/149‑1 (MQ=255)
                                                     gCAAAAAGCGCGGGTAAGTCGGCTGTATGGCTTTCCCGCCGGGCGGCAACCCGTTCGACCACGCCATGACGGTCAGCACCTGACCGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGCCCTGAGcggcatc                                                                                                <  2:4269290/149‑1 (MQ=255)
                                                         taatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggca                                                                                            >  1:1587725/1‑149 (MQ=255)
                                                          aatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                                                           >  1:1464210/1‑149 (MQ=255)
                                                             ggcgGTTATTTCGGCTTTATTGCTTCCCCGCTGGGCGGCAATGGTTTCTACACGGGCATGACGTTCAGAAATTGAACGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                                                                        <  2:3485357/148‑1 (MQ=255)
                                                             cgcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAACGCGTTCGCCAACGGCATGAGGTTCAGCAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                                                                        <  2:2158959/149‑1 (MQ=255)
                                                             cgcgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                                                                        >  2:1094190/1‑149 (MQ=255)
                                                               cgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttg                                                                                      >  2:4336612/1‑149 (MQ=255)
                                                                  ttAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                                                                   >  2:2369662/1‑149 (MQ=255)
                                                                   tAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcctt                                                                                  >  1:916260/1‑149 (MQ=255)
                                                                      ttCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                                                               >  2:776531/1‑149 (MQ=255)
                                                                              ttttGGTTTCCTGCTGTGCGTCAATGGGTTCTACACCGGCTTGACGTTCACCAAATGAACGCGGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACAcc                                                                       <  1:2523649/147‑1 (MQ=255)
                                                                               aTTGCTTTCCAGCAGTGCGGCAACGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                      <  2:4164695/149‑1 (MQ=255)
                                                                               aTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                      >  2:1694553/1‑149 (MQ=255)
                                                                               aTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                      >  1:1659245/1‑149 (MQ=255)
                                                                               aTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                      >  1:132937/1‑149 (MQ=255)
                                                                               aTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                      >  1:650038/1‑149 (MQ=255)
                                                                                  gcgttCCAGCCGTGCGGCAAAGCGTTCGACACCGGCAGGACGTTCAGCAAATGAACGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGc                                                                   <  2:1299224/146‑1 (MQ=255)
                                                                                     ttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCg                                                                >  1:2436761/1‑149 (MQ=255)
                                                                                     ttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCg                                                                >  1:1698901/1‑149 (MQ=255)
                                                                                       ccAGCTGTGCGGAAATGCGTTCTACACCGGCTTGACTTACAGAAAATCAAAGCCGCGCCCCGCGCGCAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                                                              <  1:3614431/149‑1 (MQ=255)
                                                                                        cagcagTGCGGCAACGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa                                                             <  2:1023229/149‑1 (MQ=255)
                                                                                        cagcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa                                                             <  2:1075341/149‑1 (MQ=255)
                                                                                          gcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc                                                           >  2:2402562/1‑149 (MQ=255)
                                                                                          gcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc                                                           >  1:3979089/1‑149 (MQ=255)
                                                                                           cagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCg                                                          <  2:2886239/149‑1 (MQ=255)
                                                                                                 ggCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAACCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTAc                                                    <  1:287895/149‑1 (MQ=255)
                                                                                                  gCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACg                                                   >  1:2669024/1‑149 (MQ=255)
                                                                                                  gCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACg                                                   >  2:558130/1‑149 (MQ=255)
                                                                                                    aaaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt                                                 <  2:2749935/149‑1 (MQ=255)
                                                                                                    aaaGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt                                                 >  1:3345820/1‑149 (MQ=255)
                                                                                                      tggtttCTACAACGGATTGACTTTCAGAAATTGAAAGCGGCCCCCCGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTc                                               <  1:3226131/145‑1 (MQ=255)
                                                                                                      aGCGTTCGACAACGGCATTACGTTCAGCAAATTAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTc                                               <  1:2109356/149‑1 (MQ=255)
                                                                                                          ttCGACACCGGCTTGACTTACAGAAATTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCacac                                           <  1:1761953/149‑1 (MQ=255)
                                                                                                            cGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACAcgc                                         <  2:4455961/149‑1 (MQ=255)
                                                                                                               cAAGGGCATCACTTTCACAAAATTAAAGCCGCCCCCCGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGAt                                      <  1:107922/149‑1 (MQ=255)
                                                                                                               cAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGAt                                      >  1:1858662/1‑149 (MQ=255)
                                                                                                                aaCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATg                                     >  2:1749162/1‑149 (MQ=255)
                                                                                                                aaCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATg                                     >  2:2944085/1‑149 (MQ=255)
                                                                                                                aaCGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATg                                     >  2:2943956/1‑149 (MQ=255)
                                                                                                                     cATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCACGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGccc                                >  1:1379544/1‑149 (MQ=255)
                                                                                                                       tGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTg                              >  2:2813695/1‑149 (MQ=255)
                                                                                                                        gACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTgg                             >  2:3650144/1‑149 (MQ=255)
                                                                                                                         aCGTTCAGCAAATGAAAGCCGCGCCCAGCGCAGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGc                            <  1:1895802/149‑1 (MQ=255)
                                                                                                                           gTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGccc                          >  1:804143/1‑149 (MQ=255)
                                                                                                                             tcacaaaaTCAAAGCCGCGCCCAGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGt                        <  1:23102/144‑1 (MQ=255)
                                                                                                                               aGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTa                      >  2:2705638/1‑149 (MQ=255)
                                                                                                                               aGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTa                      >  2:3106212/1‑149 (MQ=255)
                                                                                                                                gCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTAt                     >  2:458009/1‑149 (MQ=255)
                                                                                                                                gCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTAt                     >  1:3292946/1‑149 (MQ=255)
                                                                                                                                gCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTAt                     >  1:3293246/1‑149 (MQ=255)
                                                                                                                                   aaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                  >  1:298929/1‑149 (MQ=255)
                                                                                                                                   aaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                  >  1:45377/1‑149 (MQ=255)
                                                                                                                                    ttcaacgccgccCCCCGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                 <  1:1255584/143‑1 (MQ=255)
                                                                                                                                    aTGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                 <  1:4450930/149‑1 (MQ=255)
                                                                                                                                    aTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                 <  1:4557539/149‑1 (MQ=255)
                                                                                                                                     ttaacgCCGCGCCCTGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGt                <  1:4289314/144‑1 (MQ=255)
                                                                                                                                     tGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGt                >  1:235984/1‑149 (MQ=255)
                                                                                                                                     tGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGt                <  2:3735482/149‑1 (MQ=255)
                                                                                                                                     tGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGt                >  2:3426733/1‑149 (MQ=255)
                                                                                                                                      gAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTc               <  1:3798869/149‑1 (MQ=255)
                                                                                                                                       aaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCa              >  2:1188088/1‑149 (MQ=255)
                                                                                                                                        aaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAc             >  2:867964/1‑149 (MQ=255)
                                                                                                                                        aaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAc             >  1:2500537/1‑149 (MQ=255)
                                                                                                                                           ccGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGt          <  2:33300/149‑1 (MQ=255)
                                                                                                                                           ccGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGt          <  2:4144137/149‑1 (MQ=255)
                                                                                                                                                cccAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATc     <  2:44607/149‑1 (MQ=255)
                                                                                                                                                 ccAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATcc    >  2:1787897/1‑149 (MQ=255)
                                                                                                                                                 ccAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATcc    >  1:2032750/1‑149 (MQ=255)
                                                                                                                                                 ccAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATcc    >  2:4095729/1‑149 (MQ=255)
                                                                                                                                                 ccAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATcc    <  2:1436546/149‑1 (MQ=255)
                                                                                                                                                   aGCGCGGAAACGCCGCACTGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAt  >  2:616826/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAT  >  CP009273/1823688‑1823983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑