Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,757 T→A 9.2% intergenic (‑74/+234) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7570TA 9.2% 571.3 / 3.9 190intergenic (‑74/+234)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base T (114/55);  new base A (5/15);  total (119/71)
Fisher's exact test for biased strand distribution p-value = 3.64e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

TATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCT  >  CP009273/2170611‑2170905
                                                                                                                                                    |                                                                                                                                                    
tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATca                                                                                                                                                    <  1:609988/149‑1 (MQ=255)
      aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATAGAG‑ATAACGATCACaaaaa                                                                                                                                              >  2:4570766/1‑149 (MQ=255)
         ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGa                                                                                                                                           <  2:1343753/149‑1 (MQ=255)
           ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTCAAAATATAATTTAATTATTTTTTTTTAAATATAGAG‑ATAAAGATCAAAAAAAAGaaa                                                                                                                                         >  2:1083198/1‑147 (MQ=255)
            gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTGTTTT‑GTTTTAAAAATATAAATAAAATATTTTTTTTTTTATATATAT‑ATAAAGATAACaaaaaacaaaa                                                                                                                                        >  1:3910486/1‑143 (MQ=255)
            gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTCAAAAAATAATGAAATTATTTTTTTTTAAATATCTAG‑ATAACGATCACAAAAACGACaa                                                                                                                                        >  2:2121173/1‑149 (MQ=255)
              aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTCAAAAAAAAATTTAAATATTTTTTTTTTAATATAGAT‑ATAACGATCACAAAAAAAACaaaa                                                                                                                                      >  1:1039444/1‑147 (MQ=255)
              aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGTTTTAAAAATAAAATTAAAATATTTTTTTTTTAATATAGAGAATAACGAAAACAAAAAcaacaaa                                                                                                                                       >  1:1039302/1‑148 (MQ=255)
                cgaaTAACCGCCGCGCTGTGCGTTGTTCAGCATCTTCTTTTTCGATACCACGTACATTTTCATATCCTGTCTT‑TTGTTTTCTATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCTAG‑ATAACGATCACAAAAACGACAATATg                                                                                                                                    <  2:3751493/146‑1 (MQ=255)
                cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTTAATATAGAG‑ATAAAGATCACAAAAAAGACacaatt                                                                                                                                    >  2:1662503/1‑144 (MQ=255)
                    tAACCGCCGCGCTGTGCGTTGTTCAGAATCTGATTTGTCGATACCACGTAAATTTTCATATCCTGTCGT‑TTTTTTTCTATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAG‑ATAACTATCACAAACACGACAATATGaaaa                                                                                                                                <  1:1808701/149‑1 (MQ=255)
                      aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGGTTTAGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAatt                                                                                                                              >  2:3014365/1‑149 (MQ=255)
                      aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAG‑ATAACGATCACAAAAACGACAATATGaaaaag                                                                                                                              >  2:3014556/1‑147 (MQ=255)
                             cgcTGTGCTTTGTTCAGCATCTGCTTTGTCGATACCCCGTACATTTTCATACCCTGACGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAACACGACAATATGAAAATTATTCgag                                                                                                                       <  2:3944838/149‑1 (MQ=255)
                             cgcTGTGCGTGTTTCCGCATCTGCTTTGTCGCTCCCCCGTACATTTTCATATCCTGTGGT‑TTGTTTTCGATTTCAAAATATAATGAAATTTTTTTTTTTTAAATATCGTT‑ATACCGATCACAAACACGACAATATGAAAATTATTCgag                                                                                                                       <  1:2574347/149‑1 (MQ=255)
                              gcTGTGCGTTGTTCAGCATCTGCTTTGTCTATACCACGTAATTTTTCATATCCTGTCTT‑TTGTTTTCGATTAAAAAATATAATCAATTTTTTTGTTTTTAATTATCGAT‑ATAACGATAAAAAAAACGACAATATGAAAATTATTCgagg                                                                                                                      <  1:2598972/149‑1 (MQ=255)
                               cTGTGTGTTGTTCAGCATCTGCTTTGTCGATACCACGAACATTTTCATATCCTGTCTT‑TTGTTTTCGTTTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCTAG‑ATAACGATCAAAAACACGACAATATGAAAATTATTCgagga                                                                                                                     <  1:2514839/149‑1 (MQ=255)
                                tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAATAAAAAGAAAATATTTTTTTTTTAATATAGAG‑ATAAAAATAACAAAAAACACAAAAATAAAAATATTAgaggag                                                                                                                    >  2:2779335/1‑149 (MQ=255)
                                 gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTCTATTTGAAAATATAAATAAATTATTTTTTTTTAAATATAGAG‑ATAACGATCAAAAAAAAAAAAAAATTAAAATAATTCGAGGAGt                                                                                                                   >  1:2648156/1‑149 (MQ=255)
                                    cGTTGTTCATCATCTGTTTTGTCGACACCACGTAATTTTAAATATCCTGTTTT‑TTTTTTTCTATTAAAAAATATAATTAAATTTTTTGTTTTTAAATATCGAG‑ATACCTAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGaa                                                                                                                <  1:1610032/149‑1 (MQ=255)
                                           ctgCATCTGCTTTGTCGATCCCACGTACATTTTCATAGCCTGTCGT‑TTGTGTTCGATTTCAAAATTTAATGAAATTATTTTTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                                                         <  2:4482274/147‑1 (MQ=255)
                                             gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGAGTTAAAAAAAAAAATAAAATATTTTTTTTTAAATATAGAG‑CTAACCAACACCAAAACCACAAAATGACAATTATTAGAGGCGGGCAAGGCacaaa                                                                                                       >  2:4034707/1‑149 (MQ=255)
                                             gCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGT‑TGGTTTTCGAATTAAAAATAAAATGAAAATAATTTTTTTTAAAAATCCAG‑ATAACCATCACAACAAACACAAAAAGAACATTAATGGCGGAGGGAAACGCaaaaa                                                                                                       >  2:4034812/1‑149 (MQ=255)
                                               aTCTGCTTTGTCTATACCCCGTAATTTTTCATATCCTGTCGT‑TTTTTTTCTATTACAAAATATAATTAAATTATTTTTTTTTAAATATCGAG‑ATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                                                     <  1:3783637/149‑1 (MQ=255)
                                               aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTAAAAAAAAAATTAAAATAATTTTTTTTAAATAAAGAT‑ATAAACAAAAAAAAAAAAAAAAAAATAAAAATATTAGATGTGTGAAATGCaaaaaaa                                                                                                     >  2:482781/1‑149 (MQ=255)
                                               aTCTGCTTTGCCTATACCACGAACATTTACATAACCTGTCGT‑TTTTTTTCGATTAAAAAATATAATTAAATTATTTGTTTTTAAATATCGAT‑ATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                                                     <  1:4501651/149‑1 (MQ=255)
                                                tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGTATTTAAAATATAATTAAAAAATTTTTTTTTAAATATATTT‑ATAAAGAAAAAAAAAAATAAAAAATGAAAATTATTATAGGAGTTATATTCaaaaaaaa                                                                                                    >  1:1599864/1‑148 (MQ=255)
                                                    cTTTGTCTATACCACGTAAATTTTCATATCCTGTCTT‑TTTTTTTCTATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAT‑ATAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGcc                                                                                                <  2:347075/149‑1 (MQ=255)
                                                       tgtctttACCAGTTACTTTTTATTATCCTGTGGT‑TTTTTTTGTTTTTAAAATTATATTTAATTTTTTTTTTTTTAATTATCGTT‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                                                             <  2:517434/143‑1 (MQ=255)
                                                          ctaaaacACTTAAATTTTATTATCTTTTAGT‑TTTTTTTCTATAAAAAAATAAAATTAATTTTTTTTTTTTAAAATATCTAT‑AAAAATATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                          <  1:4257293/143‑1 (MQ=255)
                                                                 aCGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAAAATAATGAAATTATTTTTTTTTAAATATAGAG‑ATAACGATAACAAAAACGACAATATTTAAATTATTCGAGGAGTGAAATGCAAAAAAACCGCCACCCGAGAGGGaa                                                                                   >  2:1836701/1‑149 (MQ=255)
                                                                       ttttaCATATCCTGTCTT‑TTTTTTTCTTTTAAAAAATATAATTAAATTATTTTTTTTTAAATATCTAT‑ATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                             <  1:2276401/148‑1 (MQ=255)
                                                                       aTTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                             <  2:2669969/149‑1 (MQ=255)
                                                                        ttttCATATCCTGTCGT‑TTTTTTTAGATTTCAAAATAAAAATAAATTATTTGTTTTTTAATATAGAG‑ATAAACATAACAAAAAACACAATATTAAAAATATTAGAGGAGTGAAAGGCAAAAAAAACGCCACCCCATAGGGAAGCCGTAg                                                                            >  2:1778090/1‑149 (MQ=255)
                                                                                       gt‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                                                             >  1:2754309/1‑149 (MQ=255)
                                                                                       gt‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                                                             >  1:3132300/1‑149 (MQ=255)
                                                                                        t‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                            >  1:1726926/1‑149 (MQ=255)
                                                                                         gttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGttt                                                            >  1:4032616/2‑149 (MQ=255)
                                                                                          tttttttCGATTAAAAAATAAAATCAATTTTTTTTTTTTTAAATATCTAT‑ATAACTAAAAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                                                           <  1:2355496/146‑1 (MQ=255)
                                                                                            tttttCGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAG‑AAAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                                                         <  2:2436368/148‑1 (MQ=255)
                                                                                             ttttCTATTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCGAT‑TTAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                        <  1:1856024/149‑1 (MQ=255)
                                                                                             ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                        >  1:2138919/1‑149 (MQ=255)
                                                                                              tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                       >  2:418159/1‑149 (MQ=255)
                                                                                               ttCGATTTCAAAATATAATGAATTTATTTTTTTTTAAATATCGTT‑ATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                      <  1:364108/149‑1 (MQ=255)
                                                                                               ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                      >  1:4371498/1‑149 (MQ=255)
                                                                                                  gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                                                   >  1:2587162/1‑149 (MQ=255)
                                                                                                   ttttAAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                                                  <  2:1996988/148‑1 (MQ=255)
                                                                                                   attaaaaaaTATAATTAAATTATTTGTTTAAAAATAACGAT‑AAACCGACCACAAACCCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                                                  <  1:1576627/144‑1 (MQ=255)
                                                                                                   aTTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                                                  <  2:675306/149‑1 (MQ=255)
                                                                                                   aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                                                  >  1:1402993/1‑149 (MQ=255)
                                                                                                   aTTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                                                  <  2:4300893/149‑1 (MQ=255)
                                                                                                    tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                                                 >  2:2903043/1‑149 (MQ=255)
                                                                                                    tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                                                 >  1:1130957/1‑149 (MQ=255)
                                                                                                       cAAAATATAATAAAATTATTTTTTTTTAAATATCGAT‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                              <  1:3739224/149‑1 (MQ=255)
                                                                                                        aaaaTATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGTAGTGAAAGGCAAAAAAACGGCCTCACGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                             >  1:2107623/1‑149 (MQ=255)
                                                                                                        aaaaTATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAAGTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCACGATAGGGAAGCCTTAGCAAAGTGCGCGTGTGTTTATGCCGGATGCgg                                             >  1:1463883/1‑149 (MQ=255)
                                                                                                        aaaaTATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                             <  2:3278633/149‑1 (MQ=255)
                                                                                                          aaTATAATGAAATTTTTTTTTTTTAAATATCGAG‑ATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                           <  1:3399544/149‑1 (MQ=255)
                                                                                                             ataATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                        >  2:193243/1‑149 (MQ=255)
                                                                                                              tacttaaaTTTTTTTTTTTTAAATATCGAG‑AAAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                       <  1:2410832/144‑1 (MQ=255)
                                                                                                              taataaATTTATTTTTTTTAAAATATCGAG‑ATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                       <  1:119362/149‑1 (MQ=255)
                                                                                                              taATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                       >  2:1588756/1‑149 (MQ=255)
                                                                                                                aTGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa                                     >  1:2471376/1‑149 (MQ=255)
                                                                                                                 tGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt                                    >  2:845468/1‑149 (MQ=255)
                                                                                                                  gAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                   >  1:923452/1‑149 (MQ=255)
                                                                                                                   aaaTTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                                  >  1:308041/1‑149 (MQ=255)
                                                                                                                    atttattTTTTTTTAAATAGCGAG‑ATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                                 <  2:1968693/147‑1 (MQ=255)
                                                                                                                    aaTTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                                 >  2:4427694/1‑149 (MQ=255)
                                                                                                                      ttattTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTg                               >  2:4047896/1‑149 (MQ=255)
                                                                                                                         tttGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGTCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCa                            >  2:696876/1‑149 (MQ=255)
                                                                                                                         tttGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGTCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCa                            >  2:3318242/1‑149 (MQ=255)
                                                                                                                            gTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                         >  2:3726981/1‑149 (MQ=255)
                                                                                                                            gTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                         >  2:303785/1‑149 (MQ=255)
                                                                                                                               tttAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa                      <  2:3384999/149‑1 (MQ=255)
                                                                                                                               tttAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa                      >  1:665902/1‑149 (MQ=255)
                                                                                                                                ttAAATATCGAT‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt                     <  2:545331/149‑1 (MQ=255)
                                                                                                                                ttAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt                     >  2:3492199/1‑149 (MQ=255)
                                                                                                                                  aaaTATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGt                   >  1:556627/1‑149 (MQ=255)
                                                                                                                                   aaTATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                  >  2:3370308/1‑149 (MQ=255)
                                                                                                                                   aaTATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                  >  2:3411220/1‑149 (MQ=255)
                                                                                                                                   aaTATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                  >  2:3411610/1‑149 (MQ=255)
                                                                                                                                   aaTAGCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                  <  2:1656179/149‑1 (MQ=255)
                                                                                                                                    atatCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAg                 >  1:318341/1‑149 (MQ=255)
                                                                                                                                    atatCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAg                 >  2:3999904/1‑149 (MQ=255)
                                                                                                                                    atatCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAg                 <  2:2273414/149‑1 (MQ=255)
                                                                                                                                     tatCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAgg                >  1:1386732/1‑149 (MQ=255)
                                                                                                                                      atCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc               >  1:749971/1‑149 (MQ=255)
                                                                                                                                       tCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa              >  1:3565643/1‑149 (MQ=255)
                                                                                                                                       tCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa              >  1:3565855/1‑149 (MQ=255)
                                                                                                                                          ag‑aTAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGa           <  2:2319956/149‑1 (MQ=255)
                                                                                                                                           g‑aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGAt          >  2:2365012/1‑149 (MQ=255)
                                                                                                                                           g‑aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGAt          >  2:2023591/1‑149 (MQ=255)
                                                                                                                                             aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATa         >  2:1622544/1‑149 (MQ=255)
                                                                                                                                              tAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa        >  1:4453029/1‑149 (MQ=255)
                                                                                                                                              tAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa        >  2:903386/1‑149 (MQ=255)
                                                                                                                                                aCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa      >  1:3216095/1‑149 (MQ=255)
                                                                                                                                                aCGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa      <  1:3899167/149‑1 (MQ=255)
                                                                                                                                                 cGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGAc     >  1:652099/1‑149 (MQ=255)
                                                                                                                                                   aTCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTCTGTAGGCATGATAAGACGc   >  1:2012489/1‑149 (MQ=255)
                                                                                                                                                   aTCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc   >  1:1572106/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCT  >  CP009273/2170611‑2170905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑