Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,835,291 T→G 10.9% C315G (TGC→GGC)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,835,2910TG10.9% 679.1 / 6.5 256C315G (TGC→GGC) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (89/128);  new base G (31/3);  total (125/131)
Fisher's exact test for biased strand distribution p-value = 1.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

TATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTA  >  CP009273/2835143‑2835439
                                                                                                                                                    |                                                                                                                                                    
    gATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTgcgc                                                                                                                                                  <  2:643183/149‑1 (MQ=255)
     aTGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGcc                                                                                                                                                 <  1:2812704/149‑1 (MQ=255)
     aTGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTAAGCCTCGCGCTGCGcc                                                                                                                                                 >  2:1741661/1‑149 (MQ=255)
      tgtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCt                                                                                                                                                <  1:3830204/149‑1 (MQ=255)
       gtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTc                                                                                                                                               <  2:1700672/149‑1 (MQ=255)
        tgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCTCTTTGAGCGATTAAGGCTCGCGCTGCGCCTcg                                                                                                                                              >  2:3580972/1‑149 (MQ=255)
          cAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTcggc                                                                                                                                            <  2:2977745/149‑1 (MQ=255)
          cAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTATCTTTCAGCGATTACGCCTCGCGCTGCGCCTcggc                                                                                                                                            >  2:4482543/1‑149 (MQ=255)
            ggCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTcggcgg                                                                                                                                          >  2:4158766/1‑149 (MQ=255)
            ggCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACCCCTCGCGCTGCGCCAcggcgg                                                                                                                                          >  1:2292486/1‑149 (MQ=255)
            ggCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTGAGCTATTACGCCTCGCGCGGCGCCGcggcgg                                                                                                                                          >  2:80007/1‑149 (MQ=255)
               gcgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGgaga                                                                                                                                       <  2:1960853/149‑1 (MQ=255)
               gcgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGgaga                                                                                                                                       <  2:1349324/149‑1 (MQ=255)
               gcgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGgaga                                                                                                                                       <  2:3318065/149‑1 (MQ=255)
                 ggggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATg                                                                                                                                     <  1:485934/149‑1 (MQ=255)
                  gggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGa                                                                                                                                    <  2:2100128/149‑1 (MQ=255)
                   ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGaa                                                                                                                                   <  2:368297/149‑1 (MQ=255)
                   ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGaa                                                                                                                                   <  2:4020076/149‑1 (MQ=255)
                   ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGaa                                                                                                                                   >  2:1059539/1‑149 (MQ=255)
                    gCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCGCGGCGGAGATGAAc                                                                                                                                  >  1:1092276/1‑149 (MQ=255)
                       cGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGcc                                                                                                                               <  2:4154753/149‑1 (MQ=255)
                           tCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTCTTTCAGAAAAAAAGCCTCGCGCGGCGCCACCGCCGCGAAGAAAGCcaaca                                                                                                                           >  1:703097/1‑149 (MQ=255)
                              ggCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAacaaca                                                                                                                        <  1:2624418/149‑1 (MQ=255)
                              ggCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAacaaca                                                                                                                        <  1:334162/149‑1 (MQ=255)
                                 ttACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagca                                                                                                                     <  2:2229222/149‑1 (MQ=255)
                                  tACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                    <  1:3925181/149‑1 (MQ=255)
                                  tACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                    <  1:783390/149‑1 (MQ=255)
                                  tACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCGATTAAGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                    >  2:2474320/1‑149 (MQ=255)
                                   aCTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGt                                                                                                                   <  1:242508/149‑1 (MQ=255)
                                   aCTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGt                                                                                                                   <  1:1013573/149‑1 (MQ=255)
                                      ctGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTgcg                                                                                                                <  2:1323405/149‑1 (MQ=255)
                                       tGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCGTTAAGCTATTAAGCCACGCGCTGCGCCACCGCGGAGATTAACGCCCACAACAGCAGTgcgg                                                                                                               >  1:772416/1‑149 (MQ=255)
                                         cccGCGTATTCCGCGAAAAAGGGGTAACCATCAACAACGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTgcggcg                                                                                                             <  1:535173/149‑1 (MQ=255)
                                            gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTATCTTTCAGCAATTAACCCGCGCGCTGCGCCTCGGCGGAGATTAACGCCAACAAACCCAGTGCGGCGCAc                                                                                                          >  2:4407716/1‑149 (MQ=255)
                                              gTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgt                                                                                                        <  1:3598809/149‑1 (MQ=255)
                                               tATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCCGTGCGGCGAACgtg                                                                                                       >  2:4342316/1‑149 (MQ=255)
                                               tATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtg                                                                                                       <  2:1835764/149‑1 (MQ=255)
                                               tATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCACGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtg                                                                                                       >  2:4081461/1‑149 (MQ=255)
                                                aTTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTCGTTCAGCGATTAACCCGCGCGCAGCCCCCCCGCGGCGATGAACGCCCACAAAACCACAGCGGCGGAccggg                                                                                                      >  1:3193615/1‑145 (MQ=255)
                                                 ttCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtggt                                                                                                     <  2:2168008/149‑1 (MQ=255)
                                                  tCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtggtg                                                                                                    <  2:120416/149‑1 (MQ=255)
                                                   ccGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGa                                                                                                   <  1:1371627/149‑1 (MQ=255)
                                                       gAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTATCTTTCACCTATTACGCCGCCCGCGGCGCCGCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGGGGTGaaaac                                                                                               >  1:1422357/1‑147 (MQ=255)
                                                         aaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGc                                                                                             <  1:611499/149‑1 (MQ=255)
                                                         aaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGc                                                                                             <  1:2949938/149‑1 (MQ=255)
                                                          aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCGCGCGCTGCGCCTCGGCGGAGATGAACCCCAACAACAGCAGTGCGGCGCACGGGGTGAAATAGcg                                                                                            >  2:3041172/1‑148 (MQ=255)
                                                          aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCGATTAAGCCTCGCGCGGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATAGCt                                                                                            >  2:1528133/1‑149 (MQ=255)
                                                            aGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGTCTTTAAGCGATTAAGCCTCGCGCGGCGCCACGGCGGAGTTTAAAGCCCACAACACCAGTGCGGCGCACCTGGGGAAATAGCGGc                                                                                          >  1:1217590/1‑149 (MQ=255)
                                                            aGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTGTCTTTGAGCTATTAAGCCACGCGCGGCGCCGCGGCGGCGCTGAACGCCAACAAAAGCAGAGGGGCGCCCGGGGGGaaaaatcatc                                                                                          >  1:1570346/1‑142 (MQ=255)
                                                                   aCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATcc                                                                                   <  1:3508745/149‑1 (MQ=255)
                                                                   aCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCGATTACGCCGCGCGCTGCGCCACGGCGGCGATGAACCCCAACAACAGCAGTGCGGCGAACCTGGTGAAATCGCGGCGTAATcc                                                                                   >  2:1004039/1‑149 (MQ=255)
                                                                    ccATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATGTTTTGTATTTAAGCTATTACGCCACGCCCGGCGCCGCGGCGGGGATGAAACCCCACAAAAACCGTGCGGCGCCCCTGGTGTAAAAGCTGCGTAATACg                                                                                  >  1:4384362/1‑149 (MQ=255)
                                                                      aTCAACCAAGCCCCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTa                                                                                <  1:854675/149‑1 (MQ=255)
                                                                      aTCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTGTCTTTGAGCGATTACGCCACGCGCTGCGCCGCGGCGGAGATGAAAGCCCACAAAAGCAGTGCGGCGAACGTGGTGAAATAGCTGCGGAATCCGTa                                                                                >  2:4257124/1‑149 (MQ=255)
                                                                      aTCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCTAATTCAGCAATTAAACCGCCCGCAGCGCCACGGCGGAGATTAACGCCAACAACAGCACTTCGGCGAAACTGGTGAAATCGCGGCGTAATCCgga                                                                                >  1:2575744/1‑147 (MQ=255)
                                                                        caacaaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTAATACGCCTCGCGCTGCGCCTCGGCGGTGATGAACGCCAACAACAGCAGTGCGGCGAACGGGGTGAAATCGCTGCGTAATCCGTATc                                                                              >  2:4183049/1‑149 (MQ=255)
                                                                         aacaaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCt                                                                             <  1:2854381/149‑1 (MQ=255)
                                                                          acaaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCGATTAAGCCTCGCGCGGCGCCGCGGCGGCGATGTACGCCAACAACAGCAGTGCGGCGAACGGGGTGTAATCGCGGCGTAAACCGGATCTa                                                                            >  1:4136151/1‑149 (MQ=255)
                                                                           caaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTAAGCCACGCGCGGCGCCACGGCGGGGATGAAAGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATAGCTGCGTAATCCGTATCTAc                                                                           >  1:1111060/1‑149 (MQ=255)
                                                                               gCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGt                                                                       <  1:1937583/149‑1 (MQ=255)
                                                                               gCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGt                                                                       <  1:4241292/149‑1 (MQ=255)
                                                                                 aCCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGa                                                                     <  2:3753518/149‑1 (MQ=255)
                                                                                        gatgatGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActg                                                              <  1:737914/149‑1 (MQ=255)
                                                                                        gatgatGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActg                                                              <  1:1257915/149‑1 (MQ=255)
                                                                                        gatgatGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTAAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATAGCTGCGTAATCCGTATCTACAGGTGAGCGActg                                                              >  2:1869497/1‑149 (MQ=255)
                                                                                        gatgatGAAATCCTGAAAAACACCGTCGAGTTTGTGTCTTTGAGCTATTACGCCGCGCGCGGCGCCACGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGCACGTGGTGTAATAGCTGCGTAATCCGTATCTACACGTGAGCGActg                                                              >  1:1808485/1‑149 (MQ=255)
                                                                                         atgatgAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTTCGCCACGCGCTGCGCCACGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTTTCTACAGGTGAGCGActgg                                                             >  2:3428646/1‑149 (MQ=255)
                                                                                          ttatgaAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGTAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggg                                                            <  1:4304766/147‑1 (MQ=255)
                                                                                           gatgaAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCGGCGCCACGGCGGAGATGTACGCCAACAACAGCAGTGCGGCGAACCGGGTGGAATCGCAGCGGAAACCGTATCTACAGGTGAGCGActgggg                                                           >  2:201894/1‑149 (MQ=255)
                                                                                               aaaTCCTGAAAAACACCGTCGATTTTGTATATTTCAGCGATTACGCCGCGCGCTGCGCCACGGCGGAGATGAACGCCAACAACACCAGTGCGGCGAACGGGGTGAAAAAGCTGCGTAATCCGTATCTAAAGGTGAGCGActggggctgg                                                       >  2:3252381/1‑149 (MQ=255)
                                                                                                aaTCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggctggg                                                      <  2:2632772/149‑1 (MQ=255)
                                                                                                aaTCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggctggg                                                      <  2:708016/149‑1 (MQ=255)
                                                                                                   ccTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGaa                                                   <  2:2690310/149‑1 (MQ=255)
                                                                                                         aaaCACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATc                                             <  2:685064/149‑1 (MQ=255)
                                                                                                         aaaCACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATc                                             <  2:1587799/149‑1 (MQ=255)
                                                                                                         aaaCACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATc                                             <  2:1587110/149‑1 (MQ=255)
                                                                                                            cacCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCAc                                          <  2:3914104/149‑1 (MQ=255)
                                                                                                               cGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCg                                       >  2:2450523/1‑149 (MQ=255)
                                                                                                                 tCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGt                                     >  2:4150090/1‑149 (MQ=255)
                                                                                                                 tCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCACGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATAGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCAATCGGt                                     >  1:793728/1‑149 (MQ=255)
                                                                                                                     ttttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGc                                 >  1:1972344/1‑149 (MQ=255)
                                                                                                                       ttGTCTCTTTCAGCTTTTCCGCCTCGCGCGGCGCCGCGGCGGATATGAACGCCAACACCAGCAGTGCGGCGAACGTGGTGAAAGCGCTGCTTAATCCTTATCTACTGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGt                               <  1:3081354/149‑1 (MQ=255)
                                                                                                                       ttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGt                               >  1:3451166/1‑149 (MQ=255)
                                                                                                                           ctctTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCa                           <  2:1806697/149‑1 (MQ=255)
                                                                                                                              tttCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCa                        <  2:3614418/149‑1 (MQ=255)
                                                                                                                               ttCAGCTATTCCGCCGCGCGCTGCGCCTCGGCGGATATGAACGCCAACAACCGCTGTGCGGCGAACGTGGTGAAATCGCTGCTTAATCCTTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCAt                       <  1:1532303/149‑1 (MQ=255)
                                                                                                                                 cAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGa                     <  2:1378302/149‑1 (MQ=255)
                                                                                                                                 cAGATATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACCGCAGTGCGGCTAACGTGGTTAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGa                     <  1:3287877/149‑1 (MQ=255)
                                                                                                                                  aGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGaa                    >  1:1652459/1‑149 (MQ=255)
                                                                                                                                  aGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGaa                    >  1:3576508/1‑149 (MQ=255)
                                                                                                                                   gCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAat                   >  2:3807584/1‑149 (MQ=255)
                                                                                                                                       ttACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatga               >  1:4044867/1‑149 (MQ=255)
                                                                                                                                       ttACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatga               >  1:1679971/1‑149 (MQ=255)
                                                                                                                                         aCGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgatg             <  2:472465/149‑1 (MQ=255)
                                                                                                                                          cGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGt            <  2:917315/149‑1 (MQ=255)
                                                                                                                                           gCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTa           <  2:39340/149‑1 (MQ=255)
                                                                                                                                           gCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTa           >  1:4431783/1‑149 (MQ=255)
                                                                                                                                              tCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGa        >  2:3135312/1‑149 (MQ=255)
                                                                                                                                                  gcTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGt    <  1:3730045/149‑1 (MQ=255)
                                                                                                                                                    tGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTa  <  1:1111954/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTTA  >  CP009273/2835143‑2835439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑