Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,759 T→G 6.1% V232G (GTT→GGT)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,7590TG 6.1% 611.1 / 4.8 218V232G (GTT→GGT) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (65/134);  new base G (17/2);  total (82/136)
Fisher's exact test for biased strand distribution p-value = 1.59e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

GCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTAT  >  CP009273/3080611‑3080907
                                                                                                                                                    |                                                                                                                                                    
gCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGgtcgtgtcgt                                                                                                                                                      >  1:1118395/1‑149 (MQ=255)
gCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttcgt                                                                                                                                                      <  2:36319/149‑1 (MQ=255)
gCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttcgt                                                                                                                                                      <  1:1658511/149‑1 (MQ=255)
   gTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt                                                                                                                                                   <  2:4169224/149‑1 (MQ=255)
    tCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAta                                                                                                                                                  >  2:1941324/1‑148 (MQ=255)
      gTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCgg                                                                                                                                                >  2:1474409/1‑149 (MQ=255)
        gCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTg                                                                                                                                              <  2:526301/149‑1 (MQ=255)
        gCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTg                                                                                                                                              >  2:1206106/1‑149 (MQ=255)
            tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGccc                                                                                                                                          <  1:2698368/149‑1 (MQ=255)
            tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGccc                                                                                                                                          <  1:405528/149‑1 (MQ=255)
            tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGccc                                                                                                                                          <  1:1067720/149‑1 (MQ=255)
            tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGccc                                                                                                                                          <  2:444947/149‑1 (MQ=255)
              cACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCaa                                                                                                                                        <  1:3203096/149‑1 (MQ=255)
                 tCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTTGTTTCGTTATCGGTGGCCCAATgg                                                                                                                                     <  1:819335/149‑1 (MQ=255)
                  cAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATggg                                                                                                                                    <  2:3503190/149‑1 (MQ=255)
                  cAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGGTATAGGTGGCCCAATggg                                                                                                                                    >  1:2797190/1‑149 (MQ=255)
                   aGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGt                                                                                                                                   <  2:3360140/149‑1 (MQ=255)
                     cACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGa                                                                                                                                 <  1:1168842/149‑1 (MQ=255)
                     cACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGa                                                                                                                                 <  1:3298104/149‑1 (MQ=255)
                      aCTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGAc                                                                                                                                <  2:741149/149‑1 (MQ=255)
                             agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTc                                                                                                                         <  2:2348859/149‑1 (MQ=255)
                             agagATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTc                                                                                                                         <  1:2526613/149‑1 (MQ=255)
                              gagaTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCt                                                                                                                        <  1:626796/149‑1 (MQ=255)
                                gaTCGACCAGAAATCGCTGCAAGAAGCGGTACTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGa                                                                                                                      <  2:2737773/149‑1 (MQ=255)
                                     aCCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATAGGGGGGCCAAATGGTGACTGGGGGCTGagaggt                                                                                                                 >  1:1339304/1‑144 (MQ=255)
                                         gAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTTTAGGTTGCCCAAAGGGTTGCTGCGGTCTTACTGGTCGTa                                                                                                             >  1:2450111/1‑149 (MQ=255)
                                         gAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATAGGTGGCCCAAAGGGTGACTGCGGTCTGGCTGGTCGTa                                                                                                             >  2:1157607/1‑149 (MQ=255)
                                           aaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCACATTCTTCATCAACCCGACCGGTCGTGGCGGTATAGGTGGGCCCAAGGGTGACGGCGGTCTGGCGGGTCGTgaa                                                                                                           >  1:1319876/1‑149 (MQ=255)
                                           aaTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGGaaa                                                                                                           >  2:1008157/1‑149 (MQ=255)
                                            aTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATAGGTGGCCCAAAGGGTGTCTGCGGGCTGACAGGTGGTgaaa                                                                                                          >  1:3666913/1‑149 (MQ=255)
                                               gCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAAATa                                                                                                       >  1:345020/1‑149 (MQ=255)
                                                cTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTAt                                                                                                      <  2:3144697/149‑1 (MQ=255)
                                                 tGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATc                                                                                                     <  1:2164976/149‑1 (MQ=255)
                                                 tGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGGTATCGGTGGCCCAATGGGTGGCTGCGGTCTGACTGGGCGGAAAAatata                                                                                                     >  1:1477715/1‑148 (MQ=255)
                                                   cAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGt                                                                                                   <  2:76466/149‑1 (MQ=255)
                                                   cAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGt                                                                                                   <  2:1527971/149‑1 (MQ=255)
                                                    aagaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGtt                                                                                                  <  1:1703924/149‑1 (MQ=255)
                                                    aagaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCAATGGGTGACTGCGGTCTGTCTGGTCGTAAAATTATAGtt                                                                                                  >  1:1830110/1‑149 (MQ=255)
                                                    aagaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACAAAATTCTTCATCAACCCGACCGGTCGTTTAGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGtt                                                                                                  <  1:1635344/149‑1 (MQ=255)
                                                     agaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTg                                                                                                 <  1:886561/149‑1 (MQ=255)
                                                     agaagCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTg                                                                                                 <  1:455503/149‑1 (MQ=255)
                                                         gCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGGCCCAAGGGTGTCTGCGGTCTGACGGGTCGTTAAATTATAGTTGATaa                                                                                             >  1:1629900/1‑148 (MQ=255)
                                                          cGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATAcc                                                                                            <  1:3893021/149‑1 (MQ=255)
                                                          cGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATAcc                                                                                            <  2:2813463/149‑1 (MQ=255)
                                                          cGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATAcc                                                                                            <  1:97660/149‑1 (MQ=255)
                                                              aaTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAcg                                                                                        <  2:3454108/149‑1 (MQ=255)
                                                              aaTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAcg                                                                                        <  2:48995/149‑1 (MQ=255)
                                                              aaTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTGGTTATAGGTGGGCCAATGGGTGTCTGCGGTCTGACTGGTCGTAAAAATATCGTTGATAACTaag                                                                                        >  1:1683905/1‑147 (MQ=255)
                                                               aTGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACGGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAcgg                                                                                       >  2:4073663/1‑149 (MQ=255)
                                                                tGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAcggc                                                                                      <  1:864667/149‑1 (MQ=255)
                                                                tGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTAcggc                                                                                      <  2:1541353/149‑1 (MQ=255)
                                                                tGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGGCCCCATGGGTGACGGCGGGCTGACTGGTCGTAAAATTATCGTTTGTAACTAcggc                                                                                      >  2:3884782/1‑149 (MQ=255)
                                                                 ggAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGATAACTAcggcg                                                                                     >  2:2976649/1‑149 (MQ=255)
                                                                    agagATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCa                                                                                  <  1:3138608/149‑1 (MQ=255)
                                                                    agagATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATAGGTGGCCCCATGGGTGTCGGCGGTCTGACAGGTCGTAAAATTATCGGTGATACCAACGGCGGCa                                                                                  >  1:3808444/1‑149 (MQ=255)
                                                                     gagaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCAt                                                                                 <  1:2963152/149‑1 (MQ=255)
                                                                     gagaTCATCAAGCCAAGTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCGTCATCAACCCGACCGGTCGGTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCAt                                                                                 <  2:4520308/149‑1 (MQ=255)
                                                                      agaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATg                                                                                <  2:2992224/149‑1 (MQ=255)
                                                                       gaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTTTAGGTGGCCCAATGGGTGTCTGCGGTCTGTCTGGTCGTGAAATTATAGTTTATACCAACGGCGGCATgg                                                                               >  1:2212290/1‑149 (MQ=255)
                                                                          catcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTTATACCTACGGCGGCATGgcgc                                                                            >  2:700942/1‑149 (MQ=255)
                                                                           atcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGATACCTACGGCGGCATGGcgcg                                                                           >  2:288972/1‑149 (MQ=255)
                                                                           atcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAAGTCTTCATCAACCCGACCGGTCGTTTCGGTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGcgcg                                                                           <  1:2069383/149‑1 (MQ=255)
                                                                            tcaAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGt                                                                          <  2:4401506/149‑1 (MQ=255)
                                                                              aaGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACGTGTCGGAAAAATATCGTTGTTACCTACGGCGGCATGGcgcggcc                                                                        >  1:4498952/1‑146 (MQ=255)
                                                                              aaGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGGTATCGGTGGCCCCATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCAACGGCGGCATGGCGCGGCa                                                                        >  2:2364664/1‑149 (MQ=255)
                                                                               aGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCAc                                                                       >  2:3361615/1‑149 (MQ=255)
                                                                                  cAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGt                                                                    >  2:2993006/1‑149 (MQ=255)
                                                                                  cAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGGGGTTATCGGTGGGCCAAATGGTGTATGCGGTCTGTCTGGTCGTAAAATTATCGTTGATAACTACGGCGGCATGGCGCGTCACggg                                                                    >  2:874456/1‑148 (MQ=255)
                                                                                    aTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCCAAGGGTGACTGCGGTCAGTCTGGTCGTAAAATTATCGTTGATAACAACGGCGGCCTGGCGCGTCACCGTgg                                                                  >  1:4570105/1‑149 (MQ=255)
                                                                                     ttCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAAAGGGTGACTGCGGTCTGTCTGGTCGTAAAATTATAGTTGATACCTAAGGCGGCATGGCGCGTCACGGTGGc                                                                 >  2:2469310/1‑149 (MQ=255)
                                                                                       cTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCgg                                                               <  2:1705124/149‑1 (MQ=255)
                                                                                       cTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCgg                                                               <  2:3790590/149‑1 (MQ=255)
                                                                                         gCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTg                                                             <  1:301389/149‑1 (MQ=255)
                                                                                         gCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATAGTTGATACCAACGGCGGCATGGCGCGTCACGGTGGCGGTg                                                             >  2:961921/1‑149 (MQ=255)
                                                                                            cGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTGATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTACAt                                                          <  1:3208239/149‑1 (MQ=255)
                                                                                               tGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATtct                                                       <  1:1290622/149‑1 (MQ=255)
                                                                                                 aaTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCGCGGTGGCGGTGCATTctct                                                     <  1:3145193/149‑1 (MQ=255)
                                                                                                  aTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTg                                                    <  1:3495511/149‑1 (MQ=255)
                                                                                                  aTGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTg                                                    >  2:1490750/1‑149 (MQ=255)
                                                                                                   tGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCCATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTgg                                                   >  2:2178493/1‑149 (MQ=255)
                                                                                                    ggCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGt                                                  <  1:1486475/149‑1 (MQ=255)
                                                                                                     gCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTa                                                 <  2:3045942/149‑1 (MQ=255)
                                                                                                       tGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTGGTTATCGGTGGGCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTTATAAATAAGGCGGCATGGGGCGTAACGGTGGCGGTGCATTCTATGGTaaa                                               >  2:4372988/1‑149 (MQ=255)
                                                                                                       tGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTaaa                                               <  2:2670514/149‑1 (MQ=255)
                                                                                                       tGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTaaa                                               <  2:4498600/149‑1 (MQ=255)
                                                                                                        gACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAg                                              <  1:3926000/149‑1 (MQ=255)
                                                                                                          cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGAt                                            <  1:2364331/149‑1 (MQ=255)
                                                                                                          cTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCAATTGGTGACTGCGGTCTGACTGGTCGTAAAATTATATTTTGTAACTAAGGCGGCATGGCGCCTCACGGTTGCGGTGCATTCTCTTGTAAatat                                            >  1:2247537/1‑149 (MQ=255)
                                                                                                            tCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATcc                                          <  1:3329547/149‑1 (MQ=255)
                                                                                                              tGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCAt                                        <  2:1326861/149‑1 (MQ=255)
                                                                                                                  accaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATTGGTGACTGCGGTCTGACTGGTCGTAAAAATAACGTTGATACCTACGGCGGCATGGCGCGTCACCGTGGCGGTGCAATCTCTGGTAAAGATCCAtacaa                                    >  2:3751144/1‑145 (MQ=255)
                                                                                                                  accaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATAGGTGGCCCACTGGGTGACTGCGGTCTGACGGGTCGTAAAATTATCGTTGATACCAACGGCGGCCTGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATACATCaaa                                    >  2:3750547/1‑149 (MQ=255)
                                                                                                                   ccaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCaaaa                                   <  2:1283047/149‑1 (MQ=255)
                                                                                                                     aaaTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCATGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGt                                 <  2:990559/149‑1 (MQ=255)
                                                                                                                     aaaTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGt                                 <  2:1810683/149‑1 (MQ=255)
                                                                                                                        ttcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGa                              <  1:151230/149‑1 (MQ=255)
                                                                                                                         tcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAc                             <  1:4366356/149‑1 (MQ=255)
                                                                                                                         tcttcATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGAc                             <  2:3199983/149‑1 (MQ=255)
                                                                                                                             catcaACCCGACCGGTCGTGTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGtt                         <  1:2024666/149‑1 (MQ=255)
                                                                                                                                         cGGTCGTGTCGTTATAGGTGGCCCAAAGGGTGACAGCGGTCTGACTGGTCGTAAAAATATACTTGATAACAAAGGCCGCAAGGCGCGTCACGGTGGCGGTGCAATAACAGGTAAACATCCAAAAAAAGTGGAACCTTCCGCACCCTaac             >  2:2821172/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTAT  >  CP009273/3080611‑3080907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑