Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,525,232 T→A 12.0% L189F (TTA→TTT)  yhgE ← DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,525,2320TA12.0% 774.5 / 10.0 256L189F (TTA→TTT) yhgEDUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (72/136);  new base A (36/2);  total (118/138)
Fisher's exact test for biased strand distribution p-value = 8.41e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

TAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTAT  >  CP009273/3525084‑3525380
                                                                                                                                                    |                                                                                                                                                    
tAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCt                                                                                                                                                      <  1:843631/149‑1 (MQ=255)
 aaaCGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACAGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCCGACCTa                                                                                                                                                     >  1:3010985/1‑149 (MQ=255)
  aaCGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCCCCCGCGCGGTAACCAGACCaaa                                                                                                                                                    >  1:2261718/1‑149 (MQ=255)
  aaCGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGGGAAAGTTTTCGCGCGAGGAACACCCCCCGCGCGGTAAACCGACCaaa                                                                                                                                                    >  1:4351249/1‑149 (MQ=255)
  aaCGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACCTGACAGTGTTCCCGCGACGATCACCGCCCCCCCGGTAAACAGACCaaa                                                                                                                                                    >  1:4561803/1‑149 (MQ=255)
    cGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACg                                                                                                                                                  <  2:770485/149‑1 (MQ=255)
     ggCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGt                                                                                                                                                 <  2:3971640/149‑1 (MQ=255)
       cAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTga                                                                                                                                               <  1:3279639/149‑1 (MQ=255)
       cAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTga                                                                                                                                               <  1:3042437/149‑1 (MQ=255)
        aGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGTGCCTGTGTTCGCGCGAGGATCACCCCCAGCGCGGGAACCACACCTAACGTgag                                                                                                                                              >  2:4238249/1‑149 (MQ=255)
         gggTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCAAAAGTgaga                                                                                                                                             >  2:2510130/1‑149 (MQ=255)
          ggTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGat                                                                                                                                            <  2:930378/149‑1 (MQ=255)
          ggTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGat                                                                                                                                            <  2:2796221/149‑1 (MQ=255)
          ggTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGat                                                                                                                                            <  1:3544926/149‑1 (MQ=255)
           gTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGata                                                                                                                                           <  1:3086289/149‑1 (MQ=255)
           gTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGata                                                                                                                                           <  2:4035512/149‑1 (MQ=255)
              atgatAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAACTGACAGTGTTAGCGCGAGGATCACCGCCACCGCGGTAACCACACCAAAACTGGTataaat                                                                                                                                        >  1:4131454/1‑149 (MQ=255)
               tgatAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGataaata                                                                                                                                       <  2:2736945/149‑1 (MQ=255)
               tgatAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACAGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAAACAGACCTAAAGTGAGataaata                                                                                                                                       >  1:2606527/1‑149 (MQ=255)
                gatAAACATCATGGTTAGCAATGATACTAACGGCAGCAACCACTTGGCGATTAACGTGAACAACTATTTAATAGAGTCGATTAAAAGTTAAAATGTTCGCGCCAGGATAACAGCCAGCGCGGTAAACAGAACAAACTTGAGattaataa                                                                                                                                      >  1:1830413/1‑149 (MQ=255)
                gatAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGataaataa                                                                                                                                      <  1:3430246/149‑1 (MQ=255)
                 atAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGAtaaataaa                                                                                                                                     <  2:47242/149‑1 (MQ=255)
                   aaaCATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAAAGTGTCTGGGGTCGCGCGAGGATCACCCCCCGCGCGGGAAACAGACCGAAAGTGAGAtaaataaaac                                                                                                                                   >  1:4259767/1‑147 (MQ=255)
                     aCATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTAGCGCCAGGATCACCCCCCCCGCGGTAAACACACCAAACGTGAGAtaaaaaaaaccc                                                                                                                                 >  1:4322907/1‑145 (MQ=255)
                        tCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCg                                                                                                                              >  2:2947049/1‑149 (MQ=255)
                          aGGGTTAGCAATTATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATTGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGATATAAATAAACCAGTCGGt                                                                                                                            <  1:165043/149‑1 (MQ=255)
                          aGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGt                                                                                                                            <  2:2666126/149‑1 (MQ=255)
                           gggTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCAAACCTGAGATAAATAAACCAGTCGGtt                                                                                                                           >  2:2490951/1‑149 (MQ=255)
                                  cAATTATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCaaaaa                                                                                                                    <  2:1550787/149‑1 (MQ=255)
                                   aaTGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAAAGTGACTGTGTTCGCGCGAGGATCACCGCCCGCGCGGTAACCAGACCTAACGTGAGATAAATAAAACAGTCGGGTGCaaaaaa                                                                                                                   >  2:1653571/1‑149 (MQ=255)
                                      gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAagag                                                                                                                <  2:1938901/149‑1 (MQ=255)
                                       aTACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAACTGAATGTGTTCGCGCGAGGATAACCGCCCGCGCGGTAACCAGACCTAACGGGAGATTAATAAAACAGTCGGTTGCAAAAAAGAGc                                                                                                               >  1:2257939/1‑149 (MQ=255)
                                           tAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCACCGCGGTAAACCCACCTAACCTGAGATAAATAAACCAGGCGGGTGCAAAAAAAAGCGGAt                                                                                                           >  2:70399/1‑149 (MQ=255)
                                           tAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTTTTCGCGCGAGGATAACCGCCCCCGCGGTAACCAGACCAAACCTGAGATAAAAAAAACACTCGGGTGCAAAAAAGAGCGTAt                                                                                                           >  1:2565976/1‑149 (MQ=255)
                                            aaCGGCAGCAACCCCGTGGCTATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTAtt                                                                                                          <  2:2372329/149‑1 (MQ=255)
                                            aaCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTAtt                                                                                                          <  2:3261884/149‑1 (MQ=255)
                                            aaCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTTAAAGTGTCTGTGTTCGCGCGAGGATCACCCCCAGCGCGGTTACCACACCGAAACTGAGGTAAATAAAACAATAGGTTGCAAAAAAGAGCGTAtt                                                                                                          >  1:1255694/1‑149 (MQ=255)
                                             aCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACCTGACTGTGGTCGCGCGCGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGGTGCAAAAAAGAGCGTATTg                                                                                                         >  2:2127741/1‑149 (MQ=255)
                                                tcagcaTCCGCTTGGCGATTAACGTTAACAACTTTTGAATATAGTAGATTACACGTGACTGTGTTCTCGGGAGGATCACCGCCATCGCTGTAACAAGACCTAACTTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGaaa                                                                                                      <  2:1702341/148‑1 (MQ=255)
                                                gcagcaACGCCGTGGCGATTAACGTGAACAACTTTTGTATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATCAACCAGTCGGTTGCAAACAAGAGCGTATTGaaa                                                                                                      <  2:4007947/149‑1 (MQ=255)
                                                   gcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTTAACGTGACTGTGTTCGCGCGAGGATCACCGCCCGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAAc                                                                                                   >  2:629305/1‑149 (MQ=255)
                                                        cccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGGGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGAt                                                                                              <  1:3514040/149‑1 (MQ=255)
                                                           gTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGcc                                                                                           <  2:2839859/149‑1 (MQ=255)
                                                               cGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCcaaca                                                                                       <  2:1078809/149‑1 (MQ=255)
                                                                 aTTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAg                                                                                     <  1:1012291/149‑1 (MQ=255)
                                                                 aTTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACAGTGTTCGCGCGAGGATAACCCCCAGCGCGGTAACCAGACCAAAAGTGAGAAAAAAAAACCAGTCGGTTTCAAAAAAGATCGTATTGAAAAAAGTGATGCcaaaaaa                                                                                     >  2:1462703/1‑148 (MQ=255)
                                                                  ttAACGTGAACAACTTTTGAATAGAGTCGAGTAAAACTGACAGTGTTGGCGCGAGGATCACCCCCCCCCCCGTAAACACACCAAAAGGGAGATAAAAAAAACACTCGGTTGGAAAAAATAACGGATTTAAAAAACTGATTCcaacaaaa                                                                                    >  1:4399217/1‑147 (MQ=255)
                                                                   tAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGtt                                                                                   <  1:610033/149‑1 (MQ=255)
                                                                   tAACGTGAAAAACTTTTGAATAGAGTCTATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACATAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGtt                                                                                   <  1:13221/149‑1 (MQ=255)
                                                                    aaCGTGAACAACTTTTTAATAGAGTCTATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGttt                                                                                  <  1:2395453/149‑1 (MQ=255)
                                                                     aCGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACAGTGTTCGCGCGAGGATAAACGCCAGCGCGGTAACCACACCAAACGTGAGATAAATAAAACAGTCGGTTTCAAAAAAGAGCGGATTGAAAAACGTTTTGCCAAAAAGTTTa                                                                                 >  2:2615192/1‑149 (MQ=255)
                                                                        tGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAaac                                                                              <  2:370625/149‑1 (MQ=255)
                                                                           acaacTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAaacaac                                                                           <  1:2004641/149‑1 (MQ=255)
                                                                            caacTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAACCAGACCAAACGTGAGATAAAAAAAACAGTCGGGTGCAAAAAAGAGCGTATTTAAAAACCTGATGCCAACAACATTAAAAAact                                                                          >  2:4178389/1‑149 (MQ=255)
                                                                               cTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACTTTAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTTATGCCAACAAGTTTAAACAactcac                                                                       <  1:2515326/149‑1 (MQ=255)
                                                                                ttttGAATAGAGTCGATTAAAACTGACAGTGTTCGCGCGAGGATCACCCCCACCGCGGTAAACAGACCAAAACTGAGATAAAAAAAACACACGGGTTCAAAAAAAAGCGTATTTAAAAAAATGATGCCAACAAATTTAaacaaaaaaca                                                                      >  2:3480999/1‑143 (MQ=255)
                                                                                 tttGAATAGAGTCTATTAAACGTGACTGTGTTCGCGCGAGTATCACCCCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAactcactc                                                                     <  2:1308708/149‑1 (MQ=255)
                                                                                 tttGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAactcactc                                                                     <  1:315922/149‑1 (MQ=255)
                                                                                     aaTAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGTATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCcaca                                                                 <  1:891121/149‑1 (MQ=255)
                                                                                      aTAGAGTCGATTAAAAGTTAATGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAAAGTGAGATAAATAAAACAGTCGGTTGCAAAAAATATCGTATTGAAAAACGTGATTACAACAAGTTTAAACAAAACACACCACAg                                                                >  2:2261589/1‑149 (MQ=255)
                                                                                             cGATTAACCGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGTTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGc                                                         <  1:4054891/149‑1 (MQ=255)
                                                                                             cGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGc                                                         >  2:2576029/1‑149 (MQ=255)
                                                                                               aTTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTACCGTGAGATAAATAAACCCGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAc                                                       <  2:1174753/149‑1 (MQ=255)
                                                                                               aTTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCAATCCACAGAAAAATCAc                                                       >  1:445565/1‑149 (MQ=255)
                                                                                                ttAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAcc                                                      >  2:3930911/1‑149 (MQ=255)
                                                                                                ttAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAcc                                                      >  2:2525776/1‑149 (MQ=255)
                                                                                                  aaaCGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAg                                                    >  1:2327283/1‑149 (MQ=255)
                                                                                                    aCGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGcc                                                  <  2:2178026/149‑1 (MQ=255)
                                                                                                      gTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCAc                                                >  1:4198234/1‑149 (MQ=255)
                                                                                                      gTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCAc                                                >  1:1301677/1‑149 (MQ=255)
                                                                                                         aCTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTc                                             >  2:3616571/1‑149 (MQ=255)
                                                                                                          cTGTGTTCGCGCGAGGATCACCGCCCGCGCGGAAACCAGACCTAACGTGTGATAAATACCCCACTCGGTTTCAAAAAATACCGTATTGAAAAACGTTATGACAACACGTTTAAACAACTCACTCCACAGTAAAAGCCCCAGCCACGTCa                                            <  1:932468/149‑1 (MQ=255)
                                                                                                           tgtgTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCaa                                           >  2:155976/1‑149 (MQ=255)
                                                                                                           tgtgTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCaa                                           >  2:3886418/1‑149 (MQ=255)
                                                                                                                tCGCGCGAGTATCACCGCCAGCGCGGTAACCAGACATAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCAt                                      <  1:3693037/149‑1 (MQ=255)
                                                                                                                  gcgctcggATCACCGCCAGCGCGTTAACCAGACCTAACGTGATATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTg                                    <  1:3858905/143‑1 (MQ=255)
                                                                                                                  gcgcgTGGATCACCGCCAGCGCGTTAACCAGACCTAAGGTGAGATAAAAAAACCAGTGGGTTGCAAAAAAGAGCGTTTTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTg                                    <  1:145878/149‑1 (MQ=255)
                                                                                                                   cgcgAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTgg                                   <  2:3184239/149‑1 (MQ=255)
                                                                                                                       aGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCgag                               >  1:4178294/1‑149 (MQ=255)
                                                                                                                            cACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaaaaa                          >  1:1086688/1‑149 (MQ=255)
                                                                                                                              ccGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATc                        >  1:3961071/1‑149 (MQ=255)
                                                                                                                                gCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCAc                      >  2:1756936/1‑149 (MQ=255)
                                                                                                                                 ccAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCAcc                     <  2:4403295/149‑1 (MQ=255)
                                                                                                                                 ccAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCAcc                     <  1:2535510/149‑1 (MQ=255)
                                                                                                                                   aGCGCGGTAACCAGACCTAACGTGATATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                   <  1:3254181/149‑1 (MQ=255)
                                                                                                                                   aGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                   >  2:2086306/1‑149 (MQ=255)
                                                                                                                                   aGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                   <  1:1664640/149‑1 (MQ=255)
                                                                                                                                     cgcgGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTa                 <  1:381264/149‑1 (MQ=255)
                                                                                                                                     cgcgGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTa                 <  1:2669934/149‑1 (MQ=255)
                                                                                                                                      gcgGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTaa                <  2:3509003/149‑1 (MQ=255)
                                                                                                                                        ggTAACCAGACCTAACGTTATATAAATAAACCAGTCGGTTTCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAata              <  2:3443646/149‑1 (MQ=255)
                                                                                                                                        ggTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAata              <  1:990252/149‑1 (MQ=255)
                                                                                                                                        ggTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAata              >  1:1861184/1‑149 (MQ=255)
                                                                                                                                          tAACCAGACCAAACGTTAGATAAATAACCCAGTCGGTTGCAAAAAATAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAAtata            <  1:1613109/149‑1 (MQ=255)
                                                                                                                                            aCCAGACCTAACGTTATATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAg          <  1:3636489/149‑1 (MQ=255)
                                                                                                                                            aCCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCCGTAATATAAg          >  1:462903/1‑149 (MQ=255)
                                                                                                                                             ccAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGt         <  2:3058288/149‑1 (MQ=255)
                                                                                                                                               aGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTAc       <  2:1417509/149‑1 (MQ=255)
                                                                                                                                                gACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACg      >  1:3424218/1‑149 (MQ=255)
                                                                                                                                                gACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACg      >  2:3343064/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTAT  >  CP009273/3525084‑3525380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑