BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,340	A→C	15.4%	Q12H (CAA→CAC) ‡	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,340	0	A	C	15.4%	548.0 / 22.4	253	Q12H (CAA→CAC) ‡	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (139/68); new base C (13/33); total (152/101)
Fisher's exact test for biased strand distribution p-value = 1.77e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

ATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822193‑3822488

ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                     <  2:3466910/149‑1 (MQ=255)

 taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    >  2:2651811/1‑149 (MQ=255)

     aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                >  2:3176223/1‑149 (MQ=255)

     aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                <  1:3745782/149‑1 (MQ=255)

     aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTAGATGTCTGTTTCAACCCTCGAGTCAGACAATTGGCaaacaact                                                                                                                                                >  1:2419425/1‑143 (MQ=255)

       aGTGCCTTTATCTCAATTAAATATTTAGCTGAGGATGTGGCACACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgc                                                                                                                                              <  1:3853500/149‑1 (MQ=255)

             tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                        >  2:2867723/1‑149 (MQ=255)

             tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGAc                                                                                                                                        >  2:1492300/1‑149 (MQ=255)

             tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTAACACGGGATTGTTTTCGATGTCTGTTTCCACCCACGAGTCAGAAAATTCGCAACCGGGTGCGCAGaa                                                                                                                                        >  2:3018389/1‑148 (MQ=255)

               tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCACATGCGCAGaaac                                                                                                                                      >  1:1553921/1‑146 (MQ=255)

                 tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGGGGCGCACACTCaa                                                                                                                                    >  1:3992194/1‑149 (MQ=255)

                 tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCCCTTGCGCCGAATCaa                                                                                                                                    >  1:1944481/1‑149 (MQ=255)

                   tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACaaaa                                                                                                                                  >  1:2960991/1‑149 (MQ=255)

                    cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGACTGTTTCCACCCTCGAGACAGAAAATGCGCAACACGTAGAGCAAACACaaaaa                                                                                                                                 >  1:1066320/1‑148 (MQ=255)

                      tttAAATATTTAGATGAGGTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAg                                                                                                                               <  2:2121227/149‑1 (MQ=255)

                      tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGATGCGCAGACACaaaaaaa                                                                                                                               >  1:851223/1‑146 (MQ=255)

                      tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACat                                                                                                                               >  2:805111/1‑148 (MQ=255)

                       ttAAATATTTAGTTGTGGATGTGGCAAACGTTTGATTTTCTCTTTCGGTCAGCTAAATTGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                                                                                                              <  2:2717411/149‑1 (MQ=255)

                        tAAATATTTAGATGAGGCTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACcgcg                                                                                                                             >  2:2895229/1‑149 (MQ=255)

                          attatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTGGCTACCAGGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGaa                                                                                                                           <  2:4443567/147‑1 (MQ=255)

                            tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGAAAAAAAAAACCGAAca                                                                                                                         >  1:937995/1‑148 (MQ=255)

                            tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTGTCGCAGACTCAAAACacccaaaa                                                                                                                         >  1:939111/1‑142 (MQ=255)

                             atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACGAAAAAAAGCGAAcag                                                                                                                        >  1:303420/1‑147 (MQ=255)

                              tttAGATGTGTTTGTGTAAAACTTTTTCTTTTCTATGTCGGTCACATAAAATGCCCGCTTTGCTACCCCGGTTTTGTTTTCTATTTTTTTTACCACCCTCGATTCATAATATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                                       <  1:4591014/149‑1 (MQ=255)

                              tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAACCAACTGa                                                                                                                       >  1:3234294/1‑149 (MQ=255)

                                tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGAtt                                                                                                                     >  2:2772869/1‑149 (MQ=255)

                                tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACACCCAACTGAtt                                                                                                                     >  2:1141532/1‑149 (MQ=255)

                                 aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCCGGTGCGCACACACAAAAAACCGaaaagaatt                                                                                                                    >  1:2378351/1‑142 (MQ=255)

                                  gatgtggatgtgGAAAACTTTTGCTTTTCTCTTTCGGTCAGCTAAAATCCCCGCTTTGATACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTAAGAAAATGCGCAACCGTTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                   <  2:139280/149‑1 (MQ=255)

                                   aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATTGGGAACCCGTGGCGCAGACACAAAAAAACGAACAGATTTaa                                                                                                                  >  1:3535789/1‑148 (MQ=255)

                                   aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                  >  2:2205269/1‑149 (MQ=255)

                                    tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAcc                                                                                                                 >  2:4160611/1‑149 (MQ=255)

                                       ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTc                                                                                                              >  2:522861/1‑149 (MQ=255)

                                         aTGTGGCAAACTTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                            <  2:2793928/149‑1 (MQ=255)

                                         aTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGCTTTGCTACCACGGGTTTTTTTTCGTTTTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                            <  2:613991/149‑1 (MQ=255)

                                            tgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                         <  2:2583105/149‑1 (MQ=255)

                                                aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                     >  2:2934447/1‑149 (MQ=255)

                                                     tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                                >  2:1049786/1‑149 (MQ=255)

                                                     tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                                >  2:4406432/1‑149 (MQ=255)

                                                     tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAATGATTTAACGTCTTGAAGATCGTccg                                                                                                >  2:129050/1‑149 (MQ=255)

                                                          ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACCTCTTTAAGATCGTCCGCCGCt                                                                                           >  1:1771703/1‑149 (MQ=255)

                                                            ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTTCAGTCGTGTATATAGTACGCCGCtta                                                                                         >  1:194887/1‑148 (MQ=255)

                                                              ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       >  2:1442491/1‑149 (MQ=255)

                                                              ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTACt                                                                                       >  2:3680837/1‑149 (MQ=255)

                                                               tttttCGGTCAGATAAACTGCCCGCTTGGCTACCGGGGGTTTTTTTTCGATGTGTGTTGCCCCCCGGGAGTCATAAACTGCGCACCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTc                                                                                      <  1:4107757/147‑1 (MQ=255)

                                                                     ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACGCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                >  2:1485727/1‑149 (MQ=255)

                                                                       tCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTg                                                                              <  2:3632326/149‑1 (MQ=255)

                                                                        cAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                             <  2:4316326/149‑1 (MQ=255)

                                                                         aGCTAAAATGCCCGCTTAGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCAGGTTGAGCAGAATCAAAACAGCGAACAGATAAACCGTCTTGAAGATCGTCCGCCGCGTACACAAACACTGtt                                                                            >  2:2342130/1‑149 (MQ=255)

                                                                          gCTAAACGGCCCGCTTTGCTCCCACGGGATTGTTTTCGATTTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  2:1718401/149‑1 (MQ=255)

                                                                           caaaaaGGCCGGTTTTGCTACCAGGGGTTTGTTTTCTTTTTCTGTTACCACCCTCGCGTCAGAAATGGCGCACCGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTg                                                                          <  1:2108622/147‑1 (MQ=255)

                                                                              aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccg                                                                       >  2:3180593/1‑149 (MQ=255)

                                                                              aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCGGTTTgccg                                                                       >  2:1590915/1‑149 (MQ=255)

                                                                               aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                      >  2:350155/1‑149 (MQ=255)

                                                                               aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                      >  2:382780/1‑149 (MQ=255)

                                                                                aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTgccgcc                                                                     >  2:1699606/1‑149 (MQ=255)

                                                                                 tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAAAACCGAACATCTTTAACCTCATTAATATCGGCCCCCCCGTCCCCAAAACCACTTTtccgcca                                                                    >  1:4316701/1‑148 (MQ=255)

                                                                                  gCCCGCTTTGCTACCACGGGTGTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTg                                                                   <  2:699141/149‑1 (MQ=255)

                                                                                    ccGTTTTGCTACCTGGTGTTTTTTTTCGTGGTCTGTACCCACCCCCCACACAAAAAAGGCGCACCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTc                                                                 <  1:2698202/149‑1 (MQ=255)

                                                                                    ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTc                                                                 >  2:1822678/1‑149 (MQ=255)

                                                                                      gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTTCCGCCTGTCAg                                                               >  2:2214845/1‑149 (MQ=255)

                                                                                       cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                              <  2:355302/149‑1 (MQ=255)

                                                                                       atttGCTACCAGGGGAGTGTTTTCGATGTCTGTTTCCACCCACGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                              <  2:2050406/148‑1 (MQ=255)

                                                                                        tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                             <  2:4586789/149‑1 (MQ=255)

                                                                                          tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                           >  2:333976/1‑149 (MQ=255)

                                                                                           gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                          <  2:329128/149‑1 (MQ=255)

                                                                                             tACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCCAACTTATTTACAGTCTTTAAGATCGTCCGCCCCATCCACAAACCCAGTTTTACGCCAGTcatcatattc                                                        >  1:1644406/1‑145 (MQ=255)

                                                                                                 aCGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGCGTCAGAAAAGGCGCACCGGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGc                                                    <  2:2707190/149‑1 (MQ=255)

                                                                                                 aCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGAAACAAAAAAGCCAACAGATTTAACGTCTTTAAAATAGTACGCCGCTTCCTCAAACCCAGTTTTCCGCCAGTCAGCAACTGCTGGc                                                    >  1:500680/1‑149 (MQ=255)

                                                                                                 aCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCAGACACAAAAAAAACAACTGATTTACCGTCTTTAAGATAGTCCGCCGCGTACACCAAACCAGTTTGGCGCCTGTCAGCAACTGCTGGc                                                    >  1:2791868/1‑149 (MQ=255)

                                                                                                   gggTTTGTTTTCGTGGTCTGTTTCCCCCCGCGTGTAAGAAATTGCGCACCCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                  <  1:3617281/149‑1 (MQ=255)

                                                                                                   gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                  >  2:3686001/1‑149 (MQ=255)

                                                                                                   gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                  >  2:4200631/1‑149 (MQ=255)

                                                                                                   gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                  <  2:530219/149‑1 (MQ=255)

                                                                                                    ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGGCCTCAAACCCAGTTTGCCGCCAGTCAGCATCTGCTGGCGAt                                                 >  1:4040658/1‑149 (MQ=255)

                                                                                                     ttttGTTTGCGTTGTCTTTCCCCCCCCTCGAGACCGAACACCCGCCCCCGGGGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                <  1:129067/147‑1 (MQ=255)

                                                                                                      aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                               >  2:1369878/1‑149 (MQ=255)

                                                                                                       ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCCGACACAAAAAAACGAAAAGATTTAAAGTCATTTATATAGTCCGCCGCGTCCACAAACCCGGTTTGGCGCCAGTCAGCATCTGCTGGCGATGtt                                              >  1:264414/1‑149 (MQ=255)

                                                                                                               gATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTGCCTCAAACCCTGGTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                      >  2:4278000/1‑149 (MQ=255)

                                                                                                                 tgtgtgTTTCCCCCCTCGAGTCAGAAAATGCGCAACGGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                    <  2:2406129/149‑1 (MQ=255)

                                                                                                                 tGTCTTTTTCCCCCCTCGTGTAATAAACTGCGCACCCGGTCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                    <  1:2495233/149‑1 (MQ=255)

                                                                                                                   tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  <  2:1478981/149‑1 (MQ=255)

                                                                                                                   tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  <  2:1770474/149‑1 (MQ=255)

                                                                                                                   tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCGCAAACCCTGTTTGCCGCCTGTCAGCATATGCTGGCGATGTTCGTTGCGGTGAt                                  >  2:297209/1‑149 (MQ=255)

                                                                                                                   tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACAGATTTACCGTCTTGAAGATCGTGCGCCGCTTCCTCAAACCCAGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTAGTTGCGGTGAt                                  >  2:2323562/1‑149 (MQ=255)

                                                                                                                   tCTGTTCCCACCCTCGATTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  <  2:2277562/149‑1 (MQ=255)

                                                                                                                     tGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                <  2:2376915/149‑1 (MQ=255)

                                                                                                                      gTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCGGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                               >  2:876444/1‑149 (MQ=255)

                                                                                                                       tttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACg                              >  2:1769368/1‑149 (MQ=255)

                                                                                                                        ttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                             >  2:3969728/1‑149 (MQ=255)

                                                                                                                         tCCACCCTCGCTTAATAATAGGCGCACCGGGTGCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                            <  1:1670689/149‑1 (MQ=255)

                                                                                                                         tCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                            >  2:581846/1‑149 (MQ=255)

                                                                                                                         tCCACCCGCGAGTCAGAAAAGGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                            <  2:4027239/149‑1 (MQ=255)

                                                                                                                               cTCGAGTCAGAAAATGCGCCACCCGGTGCGCCGACACAAAACACCGCACGGATTTACCGTCTTGAAGATCGTCCGCCCCGTCCTCAAACCCAGTTTGCCGCCTGTCAGCAACTGCTGGCGATGTGCGTTGCGGTGATAACGCCACCGCt                      >  1:4275477/1‑149 (MQ=255)

                                                                                                                                      cataaaaTGCGCACCCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc               <  1:3350836/146‑1 (MQ=255)

                                                                                                                                         aaaaGGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc            <  2:2437758/149‑1 (MQ=255)

                                                                                                                                          aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           >  2:3429031/1‑149 (MQ=255)

                                                                                                                                           aaTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          <  2:772740/149‑1 (MQ=255)

                                                                                                                                              gcgcACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc       <  1:219790/149‑1 (MQ=255)

                                                                                                                                                gccaCCGGTCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc     <  1:1383151/146‑1 (MQ=255)

ATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822193‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`