Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,346 T→G 8.5% V14V (GTT→GTG)  xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3460TG 8.5% 743.3 / 4.9 251V14V (GTT→GTG) xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base T (143/72);  new base G (4/26);  total (149/102)
Fisher's exact test for biased strand distribution p-value = 2.74e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

AAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTAC  >  CP009273/3822198‑3822494
                                                                                                                                                     |                                                                                                                                                     
        tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CGGTT‑GCGCAGAc                                                                                                                                              >  2:2865653/1‑149 (MQ=255)
        tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGAc                                                                                                                                              >  2:1492368/1‑149 (MQ=255)
        tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTAACACGGGATTGTTTTCGATGTCTGTTTCCACCCACGAGTCAGAAAATTCGCAAC‑CGGGT‑GCGCAGaa                                                                                                                                              >  2:3018389/1‑148 (MQ=255)
          tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CCGGT‑GCGCacacaa                                                                                                                                            >  1:1553944/1‑147 (MQ=255)
            tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACC‑CCCTT‑GCGCCGAATCaa                                                                                                                                          >  1:1944481/1‑149 (MQ=255)
             ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGACAATTCGCCAC‑CGGGT‑TCGCCCACACaaa                                                                                                                                         >  2:4214844/1‑149 (MQ=255)
               cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGACTGTTTCCACCCTCGAGACAGAAAATGCGCAAC‑ACGTA‑GAGCAAACACaaaaa                                                                                                                                       >  1:1066320/1‑148 (MQ=255)
                aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGGCAC‑CCGTT‑TGGCAGACCAAAAACa                                                                                                                                      >  1:3551278/1‑149 (MQ=255)
                 tttAAATATTTAGATGAGGATGTGGCAAACGTTTGATTTTCGCTATCGGTCAGCTAAAATGCCCGCTTTGCTACAACGGGTTTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAg                                                                                                                                     <  2:2121238/149‑1 (MQ=255)
                  ttAAATATTTAGATGTGGATGTGTCAAACTTTTGCTTTTCTCTTTCGGTCAGCTCAAATGCCCGCTTTGCTACCAGGGTTTTGTTTTCTATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCACC‑CGGTT‑GCGCAGACTCAAAACAGc                                                                                                                                    <  2:56087/149‑1 (MQ=255)
                  ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCTGTCAGCTAAAATGCCCGCTTTGCTAACACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCA‑CCGTA‑GCGCAGACTACAAACAGc                                                                                                                                    >  2:3964229/1‑149 (MQ=255)
                   tAAATATTTAGATGAGGCTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACcgcg                                                                                                                                   >  2:2895229/1‑149 (MQ=255)
                     aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCGAC‑CGGTT‑GCGCAGACTCAAAAAAACGaa                                                                                                                                 >  1:3022565/1‑149 (MQ=255)
                       tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CCGTT‑GCGCAGAAAAAAAAAACCGAAca                                                                                                                               >  1:937995/1‑148 (MQ=255)
                         tttAGATGTGTTTGTGTAAAACTTTTTCTTTTCTATGTCGGTCACATAAAATGCCCGCTTTGCTACCCCGGTTTTGTTTTCTATTTTTTTTACCACCCTCGATTCATAATATGCGCAAC‑GGGTT‑GCGCAGACTCAAAACAGCGAACTGa                                                                                                                             <  1:4591014/149‑1 (MQ=255)
                           tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTGTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACC‑CGGTT‑GCGCAGACTCAAAACAGCGAACAGAtt                                                                                                                           >  2:1141436/1‑149 (MQ=255)
                            aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACC‑CCGGT‑GCGCACACACAAAAAACCGaaaagaatt                                                                                                                          >  1:2378351/1‑142 (MQ=255)
                             gatgtggatgtgGAAAACTTTTGCTTTTCTCTTTCGGTCAGCTAAAATCCCCGCTTTGATACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTAAGAAAATGCGCAAC‑CGTTT‑GCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                         <  2:139280/149‑1 (MQ=255)
                             gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CCGTT‑GCGCAGACACAAAAAAACCAACAGAtttt                                                                                                                         >  1:4408319/1‑148 (MQ=255)
                             gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CCGGT‑GCGCAGACTAAAAACAACGACCAGATTTa                                                                                                                         >  1:3119633/1‑149 (MQ=255)
                               tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTAcc                                                                                                                       >  2:4160611/1‑149 (MQ=255)
                                 aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGGCCC‑CGGGT‑GCGCAGACTCAAAACAGCGAACTGAttttacgt                                                                                                                     >  1:3906347/1‑144 (MQ=255)
                                  ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAAAAGCGAACTGATTTACCGTc                                                                                                                    >  2:522861/1‑149 (MQ=255)
                                    ttgtgGAAATGGTTTGGTTTTGTCTTGCGGTCAGCTACAATGCCCGGTTTGCTACCTGGGGTTTGTTTTCGATGTCTGTTTCCCCCCTGGTGTCTGAATTGGCGCTAG‑GGGTT‑GGGCAGACTCAAAAGTGGGAACTGATTTACCGTCtt                                                                                                                  <  2:2793765/148‑1 (MQ=255)
                                    aTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGCTTTGCTACCACGGGTTTTTTTTCGTTTTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                                  <  2:613991/149‑1 (MQ=255)
                                       tgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                               <  2:2583163/149‑1 (MQ=255)
                                         gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGGT‑GCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTGAAGa                                                                                                             >  2:2705463/1‑149 (MQ=255)
                                              cGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTc                                                                                                        <  2:1756288/149‑1 (MQ=255)
                                                tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTccg                                                                                                      >  2:4406487/1‑149 (MQ=255)
                                                tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAAATGATTTAACGTCTTGAAGATCGTccg                                                                                                      >  2:129050/1‑149 (MQ=255)
                                                 ttGCTTTCCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCCCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCCGAAAAGGCGCAAC‑CGGTG‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgc                                                                                                     <  2:841156/149‑1 (MQ=255)
                                                    cTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCcgccgc                                                                                                  <  2:317073/149‑1 (MQ=255)
                                                      tttCTCTTCCGGTCAGATAAACGGCCCGCTTTGCAACCAGGGTTTGGTTTTGTCTGTCTTTTCCCACCCTCGTGTAATAAATGGCGC‑AC‑CGGTTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                                <  1:1779779/149‑1 (MQ=255)
                                                       ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTTAAGATCGTACGCCCCTTc                                                                                               >  2:791333/1‑149 (MQ=255)
                                                       ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGGT‑GCGCAGACTCAAAACAGCGAACTGATTTTCAGTCGTGTATATAGTACGCCGCtta                                                                                               >  1:194887/1‑148 (MQ=255)
                                                       ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGAGCACCACGGTT‑‑CGCAGACACAAAACAGCGAACAGCTTTTAAGTATTGAAGATCGTCcgcagcata                                                                                               >  1:948898/1‑146 (MQ=255)
                                                         ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                             >  2:1442491/1‑149 (MQ=255)
                                                         ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTACt                                                                                             >  2:3680837/1‑149 (MQ=255)
                                                           ctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCa                                                                                           >  2:750881/1‑149 (MQ=255)
                                                                ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACGCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                      >  2:1485727/1‑149 (MQ=255)
                                                                 gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTAGCGTGTTGAAGATCGTCCGCCGCTTGCTCAAACCCt                                                                                     >  2:428244/1‑149 (MQ=255)
                                                                  tCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTg                                                                                    <  2:3632326/149‑1 (MQ=255)
                                                                   cAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                                   <  2:4316326/149‑1 (MQ=255)
                                                                    agatAAAATGCCCGATTTGCAACCTGGTTTTTTTTTTCTTTTTCTGTTACCACCCTCTAGTCAGAAACTGCGCAAC‑GGGTG‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                                  <  1:1705458/146‑1 (MQ=255)
                                                                    aGCTAAAATGCCCGCTTAGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACC‑AGGTT‑GAGCAGAATCAAAACAGCGAACAGATAAACCGTCTTGAAGATCGTCCGCCGCGTACACAAACACTGtt                                                                                  >  2:2342130/1‑149 (MQ=255)
                                                                     gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CGGTT‑GCGCAGACGCAAAACAGCGAACTGATTTACCGGCTTGAAGATCGTCCGCCGCGTCCACAAACCCGGttt                                                                                 >  1:2626564/1‑149 (MQ=255)
                                                                      caaaaaGGCCGGTTTTGCTACCAGGGGTTTGTTTTCTTTTTCTGTTACCACCCTCGCGTCAGAAATGGCGCACC‑GGGGG‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTg                                                                                <  1:2108622/147‑1 (MQ=255)
                                                                       taatctGCCCGTTTTGCTACCTGGGTTTTTTTTTCGTGTTGTGTTTCCCCCCTCTAGTCAGAAATTGCCCACG‑CGGGT‑GCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                               <  1:3453897/144‑1 (MQ=255)
                                                                       taaatggCCCGCTTGGCGACCTGGGTTTTGTTTTCTTTGTCTGTTTCCACCGTCGAGTCACAAACGGCGCACC‑GGGGG‑GCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                               <  1:76450/143‑1 (MQ=255)
                                                                         aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccg                                                                             >  2:3180593/1‑149 (MQ=255)
                                                                         aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCGGTTTgccg                                                                             >  2:1590915/1‑149 (MQ=255)
                                                                          aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                            >  2:350155/1‑149 (MQ=255)
                                                                           aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACC‑CGGGT‑GCGCAGACACAAAACACCGAACAGATTTACCCGCTTGAAGATCGTCCGCCGCGTCCACAAACCCTGTTTggcgcc                                                                           >  1:922914/1‑149 (MQ=255)
                                                                           aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTgccgcc                                                                           >  2:1699606/1‑149 (MQ=255)
                                                                               ccGTTTTGCTACCTGGTGTTTTTTTTCGTGGTCTGTACCCACCCCCCACACAAAAAAGGCGCACC‑GGGTG‑GCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTc                                                                       <  1:2698202/149‑1 (MQ=255)
                                                                               ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CCGGG‑GCGCACACGCAAAACACCGAACAGATTTAACGGCTTGAAGATCGGCCGCCCCGTCCGCAAAACCAGGTTTCCGCCGGTc                                                                       >  1:2791358/1‑149 (MQ=255)
                                                                                 gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                                     >  2:2934254/1‑149 (MQ=255)
                                                                                 gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTTCCGCCTGTCAg                                                                     >  2:2214845/1‑149 (MQ=255)
                                                                                 gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑ACGTT‑TAGCAGACACAAAAAAACGAACAGATTTAACTACTAGAAGATCATAACCCGCGTACTCAAAACCAGATTGCCGCCAGtaaa                                                                     >  1:233267/1‑146 (MQ=255)
                                                                                  cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                                    >  2:2940764/1‑149 (MQ=255)
                                                                                  atttGCTACCAGGGGAGTGTTTTCGATGTCTGTTTCCACCCACGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                                    <  2:2050406/148‑1 (MQ=255)
                                                                                   tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                                   <  2:4586789/149‑1 (MQ=255)
                                                                                     tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                                 >  2:333976/1‑149 (MQ=255)
                                                                                      gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                                <  2:329128/149‑1 (MQ=255)
                                                                                            ccGGGATGGTTGTCGAGGTCTGTTTCCACCCTCGCGTCAGAAACTGCGCCAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGc                                                          <  2:2708018/148‑1 (MQ=255)
                                                                                              gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                        >  2:3686001/1‑149 (MQ=255)
                                                                                               ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGGCCTCAAACCCAGTTTGCCGCCAGTCAGCATCTGCTGGCGAt                                                       >  1:4040658/1‑149 (MQ=255)
                                                                                               ggAGTGTGTTCGAGGTCTGTTTCCACCCTCGAGGCAGACAATGCGCAAC‑CGGGG‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAt                                                       <  2:2358464/149‑1 (MQ=255)
                                                                                                gATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGAAACAAAACAACGAAATGATTTACCGTCTTGAAGATAGTACGCCGCTTCCACAAAACCTGTTTTCCGCCAGGCAGCAACTGCTGGCGATg                                                      >  1:4122184/1‑149 (MQ=255)
                                                                                                 aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                                     >  2:1369878/1‑149 (MQ=255)
                                                                                                     ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAC‑CGGTT‑GCGCAGACTCAAAAAAGCGAACTGATTTAAAGTCTTGAATATCGTCCGCCGCTTCCTCAAACCCTGTTTTCCGCCGGTCAGCCTCTGCTGGCGATGTTCGt                                                 >  2:1925582/1‑149 (MQ=255)
                                                                                                     ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                                 >  2:2231052/1‑149 (MQ=255)
                                                                                                            tgtctgtcCCCACCCCCGAGTCACAAAAGGCGCACC‑CGGTG‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                          <  1:2493721/149‑1 (MQ=255)
                                                                                                              tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                        <  2:1478981/149‑1 (MQ=255)
                                                                                                              tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCGGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                        <  2:1770488/149‑1 (MQ=255)
                                                                                                              tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAAAAGCGAACAGATTTACCGTCTTGAAGATCGTGCGCCGCTTCCTCAAACCCAGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTAGTTGCGGTGAt                                        >  2:2323562/1‑149 (MQ=255)
                                                                                                                tGTTTCCCCCCTCTAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                      <  2:2378351/149‑1 (MQ=255)
                                                                                                                tGTTCCCACCCTCTATACATAAAAGGCCCAAC‑CGGTT‑GCGCATACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                      <  1:335079/149‑1 (MQ=255)
                                                                                                                 gTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAAACCTGTTTGCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                                     >  2:2772280/1‑149 (MQ=255)
                                                                                                                  tttCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACg                                    >  2:1769368/1‑149 (MQ=255)
                                                                                                                    tCCACCCTCGCTTAATAATAGGCGCACC‑GGGTG‑CCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                                  <  1:1670689/149‑1 (MQ=255)
                                                                                                                    tCCACCCTCGAGTCAGAAAATGCGCACC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                                  >  2:581846/1‑149 (MQ=255)
                                                                                                                    cccACCCCCGAGTCAGAATAGGCGCCAC‑GGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                                  <  2:3551278/148‑1 (MQ=255)
                                                                                                                          cTCGAGTCAGAAAATGCGCCAC‑CCGGT‑GCGCCGACACAAAACACCGCACGGATTTACCGTCTTGAAGATCGTCCGCCCCGTCCTCAAACCCAGTTTGCCGCCTGTCAGCAACTGCTGGCGATGTGCGTTGCGGTGATAACGCCACCGCt                            >  1:4275477/1‑149 (MQ=255)
                                                                                                                           tCGAGTCAGAAACTGCGCCCC‑CGGTG‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                           <  1:495948/149‑1 (MQ=255)
                                                                                                                                tCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                      >  2:4082592/1‑149 (MQ=255)
                                                                                                                                 cAGAAAAGGCGCACC‑CGGTG‑GCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc                     <  1:1323935/149‑1 (MQ=255)
                                                                                                                                  ataaaaTGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGACAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt                    <  2:2419437/147‑1 (MQ=255)
                                                                                                                                    aaaaTGCGCAAC‑CGGTG‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc                  <  2:2441451/149‑1 (MQ=255)
                                                                                                                                     aaaTGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc                 >  2:3429031/1‑149 (MQ=255)
                                                                                                                                     aaaTGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCCCTTCCTCAAACCCTGTTTGCCGCCAGTCAGCATCAGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAACAGcc                 >  1:3662858/1‑149 (MQ=255)
                                                                                                                                           gccaC‑CGGTC‑GCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc           <  1:1383151/146‑1 (MQ=255)
                                                                                                                                              aC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg        <  1:1128574/149‑1 (MQ=255)
                                                                                                                                                 cGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGt      <  2:3824807/149‑1 (MQ=255)
                                                                                                                                                  ggTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGGTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGtt     >  1:3781258/1‑149 (MQ=255)
                                                                                                                                                   gTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGttt    >  2:4462853/1‑149 (MQ=255)
                                                                                                                                                    tt‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCTTCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTa   <  1:2867723/149‑1 (MQ=255)
                                                                                                                                                    tt‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTa   >  1:2777584/1‑149 (MQ=255)
                                                                                                                                                    tt‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTa   >  2:1146666/1‑149 (MQ=255)
                                                                                                                                                     t‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTAc  <  2:3992194/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAC‑CGGTT‑GCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTAC  >  CP009273/3822198‑3822494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑