Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,829,715 T→G 5.2% intergenic (+39/‑70) selC → / → yicT tRNA‑Sec/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,829,7150TG 5.2% 872.4 / 35.3 271intergenic (+39/‑70)selC/yicTtRNA‑Sec/pseudogene
Reads supporting (aligned to +/- strand):  ref base T (126/131);  new base G (7/7);  total (133/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 282 total aligned reads displayed.

GTAGCAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCG  >  CP009273/3829566‑3829864
                                                                                                                                                     |                                                                                                                                                     
gTAGCAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACt                                                                                                                                                       <  2:1309439/150‑1 (MQ=255)
gTAGCAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACt                                                                                                                                                       >  1:2159824/1‑150 (MQ=255)
gTAGCAATAGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACt                                                                                                                                                       <  1:284136/150‑1 (MQ=255)
   gCAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTc                                                                                                                                                    <  1:3347865/150‑1 (MQ=255)
     aaTCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCaa                                                                                                                                                  >  1:1108738/1‑150 (MQ=255)
       tCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATc                                                                                                                                                <  2:4884514/150‑1 (MQ=255)
        cGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATcc                                                                                                                                               <  1:1388578/150‑1 (MQ=255)
          aGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAg                                                                                                                                             >  2:393825/1‑150 (MQ=255)
          aGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAg                                                                                                                                             <  2:3477157/150‑1 (MQ=255)
          aGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAg                                                                                                                                             >  2:2518822/1‑150 (MQ=255)
           ggCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGa                                                                                                                                            >  1:58767/1‑150 (MQ=255)
             cgcgGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGata                                                                                                                                          >  1:5133451/1‑150 (MQ=255)
              gcgGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGatat                                                                                                                                         <  1:132853/150‑1 (MQ=255)
              gcgGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGatat                                                                                                                                         <  1:4574972/150‑1 (MQ=255)
               cgGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATAtt                                                                                                                                        >  1:2622726/1‑150 (MQ=255)
                ggAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATAttt                                                                                                                                       <  2:2061058/150‑1 (MQ=255)
                   aGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCt                                                                                                                                    <  1:1967230/150‑1 (MQ=255)
                   aGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCt                                                                                                                                    <  2:4440107/150‑1 (MQ=255)
                   aGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCt                                                                                                                                    >  1:4394736/1‑150 (MQ=255)
                      tcgtcgTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACt                                                                                                                                 <  1:2349501/150‑1 (MQ=255)
                         tcgtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCt                                                                                                                              <  1:1951508/150‑1 (MQ=255)
                         tcgtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAACGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCt                                                                                                                              <  2:1324909/150‑1 (MQ=255)
                          cgtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTc                                                                                                                             >  2:1135862/1‑150 (MQ=255)
                          cgtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTc                                                                                                                             <  2:5055932/150‑1 (MQ=255)
                           gtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCa                                                                                                                            <  2:2422902/150‑1 (MQ=255)
                           gtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCa                                                                                                                            >  1:4786046/1‑150 (MQ=255)
                           gtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCa                                                                                                                            >  1:4256289/1‑150 (MQ=255)
                           gtcTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCa                                                                                                                            >  2:2212503/1‑150 (MQ=255)
                                cGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATAtt                                                                                                                       <  2:1577648/150‑1 (MQ=255)
                                cGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATAtt                                                                                                                       >  1:4355235/1‑150 (MQ=255)
                                  gTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCa                                                                                                                     <  1:4321652/150‑1 (MQ=255)
                                   tGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCaa                                                                                                                    >  1:1777624/1‑150 (MQ=255)
                                    gAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAAt                                                                                                                   <  1:1010809/150‑1 (MQ=255)
                                     aGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATc                                                                                                                  <  2:1393596/150‑1 (MQ=255)
                                      ggcggcTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCg                                                                                                                 <  1:5069862/150‑1 (MQ=255)
                                      ggcggcTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCg                                                                                                                 <  2:5083022/150‑1 (MQ=255)
                                         ggcTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCt                                                                                                              >  2:3730672/1‑150 (MQ=255)
                                         ggcTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCt                                                                                                              >  2:1476434/1‑150 (MQ=255)
                                               aCTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGa                                                                                                        <  2:1555675/150‑1 (MQ=255)
                                                 ttCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAAt                                                                                                      >  2:2992513/1‑150 (MQ=255)
                                                 ttCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAAt                                                                                                      <  2:307321/150‑1 (MQ=255)
                                                    aaaTCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATAcc                                                                                                   >  1:2701469/1‑150 (MQ=255)
                                                    aaaTCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATAcc                                                                                                   >  1:712076/1‑150 (MQ=255)
                                                     aaTCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATAcat                                                                                                  <  1:3424242/150‑3 (MQ=255)
                                                      aTCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTg                                                                                                 >  1:4839840/1‑150 (MQ=255)
                                                        ccAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGt                                                                                               >  1:1243453/1‑150 (MQ=255)
                                                              ggggCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAgg                                                                                         >  2:3590104/1‑150 (MQ=255)
                                                              ggggCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAgg                                                                                         <  2:2346288/150‑1 (MQ=255)
                                                               gggCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAggg                                                                                        >  1:3656518/1‑149 (MQ=255)
                                                                   cgccAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTtg                                                                                    <  2:712076/150‑1 (MQ=255)
                                                                   cgccAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTtg                                                                                    <  2:511655/150‑1 (MQ=255)
                                                                     ccAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTtgtg                                                                                  <  1:1457065/150‑1 (MQ=255)
                                                                       aGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGAt                                                                                >  1:4504338/1‑150 (MQ=255)
                                                                       aGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGAt                                                                                >  1:1998246/1‑150 (MQ=255)
                                                                       aGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGAt                                                                                <  1:4099870/150‑1 (MQ=255)
                                                                          ggTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAACTGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGa                                                                             <  2:4968591/150‑1 (MQ=255)
                                                                          ggTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGa                                                                             >  1:3855475/1‑150 (MQ=255)
                                                                           gTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGAc                                                                            >  1:2051554/1‑150 (MQ=255)
                                                                              ccGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGAt                                                                         >  2:3673770/1‑150 (MQ=255)
                                                                               cGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATg                                                                        >  2:710733/1‑150 (MQ=255)
                                                                               cGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATg                                                                        <  2:760193/150‑1 (MQ=255)
                                                                                  gCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGc                                                                     <  1:4750885/150‑1 (MQ=255)
                                                                                  gCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCACAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGTGTATGTTATGGACGAAGAGc                                                                     >  1:4688806/1‑150 (MQ=255)
                                                                                   cAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCg                                                                    <  1:4012706/150‑1 (MQ=255)
                                                                                   cAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCg                                                                    <  1:1894053/150‑1 (MQ=255)
                                                                                    aGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCgg                                                                   <  1:867955/150‑1 (MQ=255)
                                                                                     ggTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGc                                                                  <  1:3191230/150‑1 (MQ=255)
                                                                                         cGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTgaa                                                              <  2:1557775/150‑1 (MQ=255)
                                                                                         cGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTgaa                                                              <  1:2828085/150‑1 (MQ=255)
                                                                                          gACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTgaag                                                             >  2:4396044/1‑150 (MQ=255)
                                                                                           aCTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTgaaga                                                            <  2:4089959/150‑1 (MQ=255)
                                                                                           aCTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTgaaga                                                            <  1:307777/150‑1 (MQ=255)
                                                                                                  tgATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGcc                                                     >  2:3360821/1‑150 (MQ=255)
                                                                                                   gATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCt                                                    >  1:1455892/1‑150 (MQ=255)
                                                                                                      cTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTc                                                 >  2:3220946/1‑150 (MQ=255)
                                                                                                       ttCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCg                                                <  1:4199618/150‑1 (MQ=255)
                                                                                                       ttCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCg                                                <  2:206893/150‑1 (MQ=255)
                                                                                                         ccGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGt                                              >  2:2759716/1‑150 (MQ=255)
                                                                                                            ccAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGttct                                           <  1:497079/150‑1 (MQ=255)
                                                                                                                aaTGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTg                                       <  1:218176/150‑1 (MQ=255)
                                                                                                                aaTGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTg                                       >  1:1707825/1‑150 (MQ=255)
                                                                                                                 aTGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGt                                      <  2:2945517/150‑1 (MQ=255)
                                                                                                                    ccTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCt                                   <  2:58767/150‑1 (MQ=255)
                                                                                                                     ctctCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTc                                  <  1:930427/150‑1 (MQ=255)
                                                                                                                        tcCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGaa                               <  1:2251999/150‑1 (MQ=255)
                                                                                                                         ccTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAAc                              <  1:2518822/150‑1 (MQ=255)
                                                                                                                         ccTGACGTTCTCCAACCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAAc                              <  1:3034818/150‑1 (MQ=255)
                                                                                                                          cTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACg                             >  2:4427280/1‑150 (MQ=255)
                                                                                                                           tGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGc                            >  1:2568302/1‑150 (MQ=255)
                                                                                                                               gTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATcc                        <  2:451908/150‑1 (MQ=255)
                                                                                                                                    ccAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGat                   >  2:429533/1‑150 (MQ=255)
                                                                                                                                     cAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGata                  >  1:2569244/1‑150 (MQ=255)
                                                                                                                                      aaCCCCTTGTAATACGGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGatat                 >  2:4307534/1‑150 (MQ=255)
                                                                                                                                        ccccTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTc               >  1:3798020/1‑150 (MQ=255)
                                                                                                                                             tGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGcc          <  2:3287915/150‑1 (MQ=255)
                                                                                                                                                aaTACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGt       <  2:1794573/150‑1 (MQ=255)
                                                                                                                                                   aCTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATg    <  2:1108738/150‑1 (MQ=255)
                                                                                                                                                    cTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATgc   <  1:1781424/150‑1 (MQ=255)
                                                                                                                                                     tGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATgcg  >  2:1882134/1‑150 (MQ=255)
                                                                                                                                                     tGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATgcg  <  1:1452627/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTAGCAATCGAGGCGCGGAAGATCGTCGTCTCCGGTGAGGCGGCTGGACTTCAAATCCAGTTGGGGCCGCCAGCGGTCCCGGGCAGGTTCGACTCCTGTGATCTTCCGCCAAAATGCCTCTCCTGACGTTCTCCAACCCCTTGTAATACTGTCAATCCAGATATTTTCTACTGCTCAATATTCAATCGGCTTCAGGAATACCTGGTCTAAGGTTTTGTGATGGACGATGAGCGGCTGAAGAATCCGCCTGTCGGTTCTTCTGTCCTCGAACGCATCCGCGATATTCACGCCAGTATGCG  >  CP009273/3829566‑3829864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑