BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,834,958	G→T	5.5%	Q48K (CAG→AAG)	nepI ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,834,958	0	G	T	5.5%	1099.7 / 26.3	287	Q48K (CAG→AAG)	nepI	putative transporter
Reads supporting (aligned to +/- strand): ref base G (158/112); new base T (13/4); total (171/116)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

GAGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTA  >  CP009273/3834809‑3835106

 agCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTgg                                                                                                                                                     <  1:2586284/150‑1 (MQ=255)

    gTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGcc                                                                                                                                                  <  2:16323/150‑1 (MQ=255)

    gTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGcc                                                                                                                                                  >  2:2081847/1‑150 (MQ=255)

          aaaaCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGc                                                                                                                                            >  1:4331359/1‑150 (MQ=255)

          aaaaCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGc                                                                                                                                            >  1:4508093/1‑150 (MQ=255)

          aaaaCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGc                                                                                                                                            >  1:1954596/1‑150 (MQ=255)

           aaaCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCg                                                                                                                                           <  1:2086747/150‑1 (MQ=255)

           aaaCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGCGTAATAAACAAACTGGCAAAAATTGCCACAAAGGCGGTCACGGTCACCGATTGCACGGCAACCCCTTCCGAAATGCCTAAATACTGGGCCATTGGCg                                                                                                                                           >  2:875982/1‑150 (MQ=255)

             aCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTc                                                                                                                                         >  1:1893389/1‑150 (MQ=255)

               atcaaaaaaaATAACAACTTAGCGGCGTTAAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACTGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTcaa                                                                                                                                       >  2:3476204/3‑150 (MQ=255)

                      aaaaTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTg                                                                                                                                <  1:1025501/150‑1 (MQ=255)

                         aTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACg                                                                                                                             <  1:4016683/150‑1 (MQ=255)

                         aTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACg                                                                                                                             <  2:2190987/150‑1 (MQ=255)

                           aacaacGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACggg                                                                                                                           <  1:512436/150‑1 (MQ=255)

                           aacaacGTAGCGGCGGTCAGTAGCCTGAAGTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACggg                                                                                                                           <  2:3708369/150‑1 (MQ=255)

                           aaaaacGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATACTGGGACATTGGCGTCAACAAACTGACggg                                                                                                                           >  1:2734133/4‑150 (MQ=255)

                               acGGAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATACACAAACTGGCAAACAGTGCCACACAGGCGGTCACGGTCACCGAGTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCaaa                                                                                                                       <  2:4141577/150‑1 (MQ=255)

                                 gTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCaaaaa                                                                                                                     >  1:3138504/1‑150 (MQ=255)

                                  tAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAAc                                                                                                                    >  2:1379483/1‑150 (MQ=255)

                                     cggcggTCAGTAGCCTGAATTGTCTGGGTAATAAACAACCTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCa                                                                                                                 <  2:1543485/150‑1 (MQ=255)

                                     cggcggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCa                                                                                                                 >  2:4922239/1‑150 (MQ=255)

                                      ggcggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCaa                                                                                                                >  2:819434/1‑150 (MQ=255)

                                      ggcggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCaa                                                                                                                <  1:3376952/150‑1 (MQ=255)

                                      ggcggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCaa                                                                                                                >  2:3720130/1‑150 (MQ=255)

                                       gcggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAAc                                                                                                               <  1:3544799/150‑1 (MQ=255)

                                        cggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACg                                                                                                              <  2:192547/150‑1 (MQ=255)

                                        cggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACg                                                                                                              >  2:4029553/1‑150 (MQ=255)

                                         ggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGa                                                                                                             >  1:364859/1‑150 (MQ=255)

                                            cAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATaa                                                                                                          >  1:1082729/1‑150 (MQ=255)

                                             aGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAAt                                                                                                         <  1:3038981/150‑1 (MQ=255)

                                               tAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCa                                                                                                       <  2:2003822/150‑1 (MQ=255)

                                                     gAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAgg                                                                                                 >  2:2486638/1‑150 (MQ=255)

                                                       aTTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCg                                                                                               >  2:2390723/1‑150 (MQ=255)

                                                       aTTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATAGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCg                                                                                               >  2:2452287/1‑150 (MQ=255)

                                                        ttGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGa                                                                                              <  1:5073759/150‑1 (MQ=255)

                                                         tGTCTGGGTAATAAACACACTGGCAAACATTGCCACACAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAACTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATACTCAGACAGGCGac                                                                                             <  2:4539230/150‑1 (MQ=255)

                                                         tGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGac                                                                                             >  1:2457969/1‑150 (MQ=255)

                                                           tCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGacac                                                                                           >  2:2996934/1‑150 (MQ=255)

                                                           tCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGacac                                                                                           >  2:2718673/1‑150 (MQ=255)

                                                           tCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGacac                                                                                           >  1:4272806/1‑150 (MQ=255)

                                                           tCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCAGGCAACCCCTTACGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACTGGCAAAAAATCAACGATAATCAGACAGGCGacac                                                                                           >  2:4404633/1‑150 (MQ=255)

                                                            cTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGAcaca                                                                                          >  1:637394/1‑150 (MQ=255)

                                                             tGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACaa                                                                                         >  2:3054790/1‑150 (MQ=255)

                                                                 taataaACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACg                                                                                     <  2:4487500/150‑1 (MQ=255)

                                                                   ataaACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGcc                                                                                   >  2:3456187/1‑150 (MQ=255)

                                                                     aaacacacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCACCCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCAc                                                                                 >  2:2769333/1‑150 (MQ=255)

                                                                     aaacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCAc                                                                                 >  1:4589591/1‑150 (MQ=255)

                                                                     aaacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCAc                                                                                 <  1:550153/150‑1 (MQ=255)

                                                                      aacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCAcc                                                                                <  2:1100176/150‑1 (MQ=255)

                                                                       acaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCg                                                                               <  1:3783564/150‑1 (MQ=255)

                                                                          aacAGGCAAACATTGCCACAAAGTCTGTCACGGTCACAGAATGCCCGGCAACCCATACCGAAATGCCTAAATCCTGCGCCAATGGCGTCAAAAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCgaga                                                                            >  2:3592682/1‑150 (MQ=255)

                                                                              ggCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAAc                                                                        >  1:4543058/1‑150 (MQ=255)

                                                                                cAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACgg                                                                      >  2:3309418/1‑150 (MQ=255)

                                                                                 aaaCATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGc                                                                     >  1:4816540/1‑150 (MQ=255)

                                                                                  aaCATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCt                                                                    <  1:3895528/150‑1 (MQ=255)

                                                                                    cATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGa                                                                  >  1:2314800/1‑150 (MQ=255)

                                                                                     aTTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGAc                                                                 >  1:1138933/1‑150 (MQ=255)

                                                                                      ttGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGAcc                                                                <  1:5011689/150‑1 (MQ=255)

                                                                                            caAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCCGTgcg                                                          >  2:2909086/1‑150 (MQ=255)

                                                                                            caAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcg                                                          <  1:2997088/150‑1 (MQ=255)

                                                                                               aGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtc                                                       >  1:2741019/1‑150 (MQ=255)

                                                                                               aGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtc                                                       >  2:4558852/1‑150 (MQ=255)

                                                                                                ggCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtcg                                                      <  1:498648/150‑1 (MQ=255)

                                                                                                 gCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtcgg                                                     >  2:4006356/1‑150 (MQ=255)

                                                                                                   ggtcacggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGt                                                   >  1:1328160/1‑150 (MQ=255)

                                                                                                   ggtcacggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGCCCATTGGCGACAACAAACTGACGGGCAAAAACTCAACGATAATCATACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGt                                                   <  2:1125872/150‑1 (MQ=255)

                                                                                                    gtcacggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTg                                                  >  1:4180657/1‑150 (MQ=255)

                                                                                                       acggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATg                                               <  1:1404168/150‑1 (MQ=255)

                                                                                                       acggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATg                                               >  2:3358606/1‑150 (MQ=255)

                                                                                                        cggtcacCGAGTGCCCGGCAACCCCTTCCGAACTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATgg                                              <  2:1194081/150‑1 (MQ=255)

                                                                                                         ggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGc                                             >  1:3147300/1‑150 (MQ=255)

                                                                                                           gcacCGCTGGCCCGGCAACCCCGTCCGACCGGCCGACCTCCGGGGCCAGTGGCGGCAACACCCGGCCGGGCAAACACTCACCGAGACTCAGACAGGCGACACCCCACGCCACCGAGAAACCGGCTGACCAGTTCGGTCGGGTGATGGCAt                                           <  2:4047755/149‑1 (MQ=255)

                                                                                                             acCGCTCGCCCCGCAACCCCCTCCCACCCGCCTAACCCCCGCGCCACCGGCGTCAACCACCTGACGGGCACCAACTCACCGATAATCAGACAGGCGACACCACCCGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATcc                                         <  2:392800/150‑1 (MQ=255)

                                                                                                              ccGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccg                                        >  1:278038/1‑150 (MQ=255)

                                                                                                                gATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcg                                      >  2:4741623/1‑150 (MQ=255)

                                                                                                                gATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcg                                      <  1:1854484/150‑1 (MQ=255)

                                                                                                                gATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAAAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcg                                      <  1:2124976/150‑1 (MQ=255)

                                                                                                                  ttGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcgcc                                    >  2:2820382/1‑150 (MQ=255)

                                                                                                                   tGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcgccg                                   >  2:4955614/1‑150 (MQ=255)

                                                                                                                   tGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcgccg                                   >  1:4315558/1‑150 (MQ=255)

                                                                                                                     cccGGCAACCCCCTCCGAACTGCCCAACTCCTGGGCCAATGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcgccgcg                                 <  2:5015897/150‑1 (MQ=255)

                                                                                                                         gCAACCCCTTCCGAACTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGttt                             <  2:1757386/150‑1 (MQ=255)

                                                                                                                         gCAACCCCGTCCGAAATGCCTAAATCCTGGGCCAGTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAACACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGttt                             <  2:4316808/150‑1 (MQ=255)

                                                                                                                          cAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGtttt                            >  2:1363798/1‑150 (MQ=255)

                                                                                                                          cAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGtttt                            <  1:1689641/150‑1 (MQ=255)

                                                                                                                              cccTTCCGAAATGCCTAAATCATGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGc                        >  2:3549411/1‑150 (MQ=255)

                                                                                                                               ccTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCa                       >  1:475289/1‑150 (MQ=255)

                                                                                                                                    cGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATaaa                  >  1:835402/1‑150 (MQ=255)

                                                                                                                                    cGAAAGGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATaaa                  <  2:5121983/150‑1 (MQ=255)

                                                                                                                                     gAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAAt                 >  2:1895192/1‑150 (MQ=255)

                                                                                                                                      aaaTGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAAtt                >  1:2813519/1‑150 (MQ=255)

                                                                                                                                      aaaTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAAtt                <  2:4472050/150‑1 (MQ=255)

                                                                                                                                       aaTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTc               >  2:1402509/1‑150 (MQ=255)

                                                                                                                                          gCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACt            >  2:2674902/1‑150 (MQ=255)

                                                                                                                                           ccTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCACAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGGTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTc           >  2:73654/1‑150 (MQ=255)

                                                                                                                                             tAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCAt         >  1:1438608/1‑150 (MQ=255)

                                                                                                                                              aaaTCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATg        >  2:1835448/1‑150 (MQ=255)

                                                                                                                                                aTCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGgt      <  2:3198808/150‑1 (MQ=255)

                                                                                                                                                 tCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGgtg     <  1:3624275/150‑1 (MQ=255)

                                                                                                                                                  ccTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGgtgt    >  1:1282873/1‑150 (MQ=255)

GAGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTA  >  CP009273/3834809‑3835106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`