BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,849,854	A→T	5.1%	H27L (CAC→CTC)	yidI →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,849,854	0	A	T	5.1%	1071.7 / 32.8	305	H27L (CAC→CTC)	yidI	inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (169/120); new base T (11/5); total (180/125)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

AGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATA  >  CP009273/3849705‑3850003

   ggTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACtt                                                                                                                                                    >  2:1104752/1‑150 (MQ=255)

    gTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACttt                                                                                                                                                   >  1:778685/1‑150 (MQ=255)

    gTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACttt                                                                                                                                                   <  2:73511/150‑1 (MQ=255)

     tttttttAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACtttt                                                                                                                                                  <  2:541917/150‑1 (MQ=255)

     tttttttAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACtttt                                                                                                                                                  <  1:3677742/150‑1 (MQ=255)

        ttttAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCAt                                                                                                                                               <  1:3353288/150‑1 (MQ=255)

          ttAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTc                                                                                                                                             <  2:2525260/150‑1 (MQ=255)

          ttAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTc                                                                                                                                             <  2:2204620/150‑1 (MQ=255)

           tAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTAGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCg                                                                                                                                            >  2:2832490/1‑150 (MQ=255)

            aaGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCgg                                                                                                                                           >  2:1872143/1‑150 (MQ=255)

            aaGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCgg                                                                                                                                           <  2:2987993/150‑1 (MQ=255)

             aGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGc                                                                                                                                          >  1:4358645/1‑150 (MQ=255)

                aTTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCt                                                                                                                                       >  1:2052946/1‑150 (MQ=255)

                aTTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCt                                                                                                                                       >  1:2574102/1‑150 (MQ=255)

                 tttCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCtt                                                                                                                                      >  2:2141198/1‑150 (MQ=255)

                     gTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTtctc                                                                                                                                  >  1:3896172/1‑150 (MQ=255)

                               ttCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCg                                                                                                                        <  1:2218925/150‑1 (MQ=255)

                               ttCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCg                                                                                                                        >  2:4576035/1‑150 (MQ=255)

                                 ccTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGcc                                                                                                                      >  2:3548979/1‑150 (MQ=255)

                                 ccTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGcc                                                                                                                      >  2:4135990/1‑150 (MQ=255)

                                    gATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAAc                                                                                                                   >  2:1971932/1‑150 (MQ=255)

                                           ttttGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGaa                                                                                                            >  1:1142764/1‑150 (MQ=255)

                                           ttttGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGaa                                                                                                            >  2:3121899/1‑150 (MQ=255)

                                            tttGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGaaa                                                                                                           <  2:2353357/150‑1 (MQ=255)

                                              tGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGc                                                                                                         >  2:749643/1‑150 (MQ=255)

                                               gCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCa                                                                                                        <  2:347854/150‑1 (MQ=255)

                                                ccATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCAt                                                                                                       >  1:4441271/1‑150 (MQ=255)

                                                ccATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCAt                                                                                                       >  1:4760905/1‑150 (MQ=255)

                                                  aTCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCg                                                                                                     >  1:1569616/1‑150 (MQ=255)

                                                   tCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAATATCAGTTCATTTGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGt                                                                                                    <  1:1312237/150‑1 (MQ=255)

                                                   tCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGt                                                                                                    <  1:3455402/150‑1 (MQ=255)

                                                        tACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCgg                                                                                               <  1:3710000/150‑1 (MQ=255)

                                                        tACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCgg                                                                                               >  1:2293609/1‑150 (MQ=255)

                                                        tACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCgg                                                                                               >  2:2315419/1‑150 (MQ=255)

                                                           aaGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACa                                                                                            >  1:1427572/1‑150 (MQ=255)

                                                           aaGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACa                                                                                            >  1:3954779/1‑150 (MQ=255)

                                                           aaGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACa                                                                                            >  2:3302086/1‑150 (MQ=255)

                                                             ggaggTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACaaa                                                                                          >  2:1500270/1‑150 (MQ=255)

                                                               aggTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAAc                                                                                        >  2:4684757/1‑150 (MQ=255)

                                                               aggTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAAc                                                                                        <  1:4390077/150‑1 (MQ=255)

                                                                ggTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACg                                                                                       >  2:897903/1‑150 (MQ=255)

                                                                 gTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACgg                                                                                      <  1:1657924/150‑1 (MQ=255)

                                                                       tGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGaaa                                                                                >  2:1927390/1‑150 (MQ=255)

                                                                        gggTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAAt                                                                               <  2:5057169/150‑1 (MQ=255)

                                                                          gTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTa                                                                             <  2:4514452/150‑1 (MQ=255)

                                                                              cATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAag                                                                         >  2:4729412/1‑150 (MQ=255)

                                                                               aTAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAaga                                                                        >  2:5037762/1‑150 (MQ=255)

                                                                                 aGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAgg                                                                      <  2:4393067/150‑1 (MQ=255)

                                                                                   cTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGtc                                                                    >  1:2813746/1‑150 (MQ=255)

                                                                                    tCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGtct                                                                   <  2:4889359/150‑1 (MQ=255)

                                                                                     cAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGtctt                                                                  >  1:1182893/1‑150 (MQ=255)

                                                                                     cAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGtctt                                                                  >  1:2414603/1‑150 (MQ=255)

                                                                                      aaaataaaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGtcttc                                                                 >  2:1288653/1‑150 (MQ=255)

                                                                                         ataaaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCg                                                              >  2:3574628/1‑150 (MQ=255)

                                                                                          taaaatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGc                                                             >  1:694881/1‑150 (MQ=255)

                                                                                             aatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCgg                                                          >  1:2186801/1‑150 (MQ=255)

                                                                                             aatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCgg                                                          <  2:3192958/150‑1 (MQ=255)

                                                                                             aatCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCgg                                                          >  1:716242/1‑150 (MQ=255)

                                                                                               tCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGaa                                                        >  2:3936342/1‑150 (MQ=255)

                                                                                               tCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGaa                                                        >  2:701070/1‑150 (MQ=255)

                                                                                                    tCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATaaaa                                                   <  1:184159/150‑1 (MQ=255)

                                                                                                      aTTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATAAAAAGAGGTCTTCTCGCCGGAATAAAAgg                                                 >  1:2904727/1‑150 (MQ=255)

                                                                                                      aTTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAgg                                                 >  1:2543921/1‑150 (MQ=255)

                                                                                                       ttGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGc                                                >  1:4103351/1‑150 (MQ=255)

                                                                                                        tGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCg                                               <  1:5079679/150‑1 (MQ=255)

                                                                                                         gggCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGa                                              >  1:1521763/1‑150 (MQ=255)

                                                                                                          ggCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGaa                                             >  1:3636155/1‑150 (MQ=255)

                                                                                                                 tGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTa                                      <  2:4469486/150‑1 (MQ=255)

                                                                                                                 tGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTa                                      >  2:4696923/1‑150 (MQ=255)

                                                                                                                 tGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTa                                      <  1:4257022/150‑1 (MQ=255)

                                                                                                                 tGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTa                                      <  2:1177493/150‑1 (MQ=255)

                                                                                                                  gTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTac                                     <  1:3134988/150‑1 (MQ=255)

                                                                                                                  gTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTac                                     >  2:4333014/1‑150 (MQ=255)

                                                                                                                     cGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTaccac                                  >  1:3849133/1‑150 (MQ=255)

                                                                                                                      ggTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTaccacc                                 >  1:1585637/1‑150 (MQ=255)

                                                                                                                      ggTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTaccacc                                 >  1:4147195/1‑150 (MQ=255)

                                                                                                                        tGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGt                               >  1:4596118/1‑150 (MQ=255)

                                                                                                                          ccATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTgg                             >  1:4726378/1‑150 (MQ=255)

                                                                                                                           cATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGga                            <  2:3698188/150‑1 (MQ=255)

                                                                                                                           cATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGga                            <  1:1104752/150‑1 (MQ=255)

                                                                                                                            aTCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGgag                           <  2:3640369/150‑1 (MQ=255)

                                                                                                                             tCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGgaga                          >  2:782108/1‑150 (MQ=255)

                                                                                                                              cGCTTTTATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGaa                         <  1:2960166/150‑1 (MQ=255)

                                                                                                                                cTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGaaaa                       >  1:3243616/1‑150 (MQ=255)

                                                                                                                                cTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGaaaa                       <  1:917513/150‑1 (MQ=255)

                                                                                                                                 tttAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGaaaaa                      <  1:1971932/150‑1 (MQ=255)

                                                                                                                                 tttAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGaaaaa                      <  2:4351407/150‑1 (MQ=255)

                                                                                                                                   tAATGATGGGGATTATCCTCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAag                    <  2:1480615/150‑1 (MQ=255)

                                                                                                                                   tAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAag                    <  2:176338/150‑1 (MQ=255)

                                                                                                                                        atgGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGAc               >  2:1337940/1‑150 (MQ=255)

                                                                                                                                        atgGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGAc               <  1:4777753/150‑1 (MQ=255)

                                                                                                                                        atgGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGAc               >  1:3002917/1‑150 (MQ=255)

                                                                                                                                           gggATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTa            >  2:643058/1‑150 (MQ=255)

                                                                                                                                            ggATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGACAAAAGAGGACGTAg           >  2:2723340/1‑150 (MQ=255)

                                                                                                                                               ttATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATg        >  1:4901092/1‑150 (MQ=255)

                                                                                                                                                tATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGt       <  1:3819841/150‑1 (MQ=255)

                                                                                                                                                tATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGt       <  1:2374470/150‑1 (MQ=255)

                                                                                                                                                 aTCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTc      >  2:855245/1‑150 (MQ=255)

                                                                                                                                                    cACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGAt   >  1:5021360/1‑150 (MQ=255)

                                                                                                                                                     aCTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATa  <  2:2384259/150‑1 (MQ=255)

AGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGCGAAAAAATTACCACCGTGGAGAAAAAAGAGGACGTAGATGTCGATA  >  CP009273/3849705‑3850003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`