BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,860,715	A→T	5.5%	L23* (TTA→TAA)	ibpA ←	heat shock chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,860,715	0	A	T	5.5%	1057.8 / 35.8	306	L23* (TTA→TAA)	ibpA	heat shock chaperone
Reads supporting (aligned to +/- strand): ref base A (147/142); new base T (9/8); total (156/150)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

TCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCG  >  CP009273/3860566‑3860864

 caccacCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTaa                                                                                                                                                      >  2:1191213/1‑150 (MQ=255)

 caccacCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTaa                                                                                                                                                      >  2:4377668/1‑150 (MQ=255)

  accaccAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAg                                                                                                                                                     <  1:3816301/150‑1 (MQ=255)

     accaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTgg                                                                                                                                                  <  2:914361/150‑1 (MQ=255)

     accaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTgg                                                                                                                                                  >  2:2291127/1‑150 (MQ=255)

      ccaGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGt                                                                                                                                                 >  2:3940175/1‑150 (MQ=255)

       cagcagATTATCCTGTGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGtt                                                                                                                                                <  2:3005769/150‑1 (MQ=255)

       cagcagATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGtt                                                                                                                                                >  1:4552021/1‑150 (MQ=255)

        agcagATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTa                                                                                                                                               <  1:3375603/150‑1 (MQ=255)

        agcagATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTa                                                                                                                                               >  1:5133819/1‑150 (MQ=255)

        agcagATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTa                                                                                                                                               <  2:3281220/150‑1 (MQ=255)

          cagATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaa                                                                                                                                             <  2:2350151/150‑1 (MQ=255)

            gATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaaca                                                                                                                                           <  1:1594810/150‑1 (MQ=255)

              ttATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTaaacaaa                                                                                                                                         >  2:3504149/1‑150 (MQ=255)

               tATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTTAGTGGTTaaacaaac                                                                                                                                        >  2:2779088/1‑150 (MQ=255)

                 tCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACgg                                                                                                                                      <  1:1696497/150‑1 (MQ=255)

                  ccTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGt                                                                                                                                     <  2:611143/150‑1 (MQ=255)

                   cTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTc                                                                                                                                    <  1:78441/150‑1 (MQ=255)

                   cTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTc                                                                                                                                    <  1:4876855/150‑1 (MQ=255)

                     gggCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCaa                                                                                                                                  >  2:4510557/1‑150 (MQ=255)

                       gCGGTAATTTCCTGTGCGCTCTCATCAAAACCATACACAGCGATAGCAATGCTGTTTTGTTTTTTGTATACCATTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTTGCTCTGGTTGTTTTCTATGTGGTTAAACAAACGGTCAAAt                                                                                                                                <  1:694096/150‑1 (MQ=255)

                       gCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTTAGTGGTTAAACAAACGGTCAAAt                                                                                                                                <  2:756214/150‑1 (MQ=255)

                         ggTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATcc                                                                                                                              >  1:4630280/1‑150 (MQ=255)

                          gTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCTATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCa                                                                                                                             <  2:4624542/150‑1 (MQ=255)

                          gTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCa                                                                                                                             >  2:1288208/1‑150 (MQ=255)

                              tttCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAg                                                                                                                         >  1:3370009/1‑150 (MQ=255)

                                 ccAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAg                                                                                                                      >  2:3585349/1‑150 (MQ=255)

                                 ccAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAg                                                                                                                      >  2:3220365/1‑150 (MQ=255)

                                   aGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGaa                                                                                                                    >  1:3670691/1‑150 (MQ=255)

                                    gTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAAc                                                                                                                   <  1:1946678/150‑1 (MQ=255)

                                      tCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACgg                                                                                                                 <  1:1284853/150‑1 (MQ=255)

                                          tctcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTaaa                                                                                                             <  2:36893/150‑1 (MQ=255)

                                           ctcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAg                                                                                                            <  2:3974384/150‑1 (MQ=255)

                                           ctcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAg                                                                                                            <  1:3343880/150‑1 (MQ=255)

                                            tcAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGc                                                                                                           <  1:4086404/150‑1 (MQ=255)

                                              aGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCgg                                                                                                         >  2:1504751/1‑150 (MQ=255)

                                              aGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCgg                                                                                                         >  2:1063156/1‑150 (MQ=255)

                                              aGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCgg                                                                                                         >  2:3860982/1‑150 (MQ=255)

                                                cAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCgggg                                                                                                       >  1:3231208/1‑150 (MQ=255)

                                                 aaaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTTAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGa                                                                                                      >  1:5018024/1‑150 (MQ=255)

                                                 aaaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGa                                                                                                      >  2:4209625/1‑150 (MQ=255)

                                                  aaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGAt                                                                                                     <  2:501693/150‑1 (MQ=255)

                                                  aaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGAt                                                                                                     <  2:2466898/150‑1 (MQ=255)

                                                  aaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGAt                                                                                                     >  2:2223137/1‑150 (MQ=255)

                                                  aaaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGAt                                                                                                     >  2:3743317/1‑150 (MQ=255)

                                                   aaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATa                                                                                                    <  1:4166328/150‑1 (MQ=255)

                                                   aaCCAGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATa                                                                                                    <  2:3070499/150‑1 (MQ=255)

                                                      cAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAAt                                                                                                 <  2:2502688/150‑1 (MQ=255)

                                                       aGCCACAGCGATAGCAATGCGGTATTGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATc                                                                                                <  1:4827772/150‑1 (MQ=255)

                                                       aGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATc                                                                                                <  1:2596113/150‑1 (MQ=255)

                                                       aGCCACAGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATc                                                                                                >  1:247309/1‑150 (MQ=255)

                                                        gCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTTAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCa                                                                                               >  1:4465432/1‑150 (MQ=255)

                                                          cacaGCGATAGCAATGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCaaa                                                                                             <  1:1922037/150‑1 (MQ=255)

                                                                gATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACg                                                                                       >  1:258976/1‑150 (MQ=255)

                                                                   aGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCAt                                                                                    <  1:125043/150‑1 (MQ=255)

                                                                      aaTGCGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAAt                                                                                 >  2:1604211/1‑150 (MQ=255)

                                                                          cGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAAt                                                                             <  2:4549829/150‑1 (MQ=255)

                                                                          cGGTAATGGTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAAt                                                                             <  2:4013196/150‑1 (MQ=255)

                                                                             tAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGc                                                                          >  2:1735777/1‑150 (MQ=255)

                                                                             tAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGc                                                                          <  2:3246143/150‑1 (MQ=255)

                                                                                 ggTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTTAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCt                                                                      >  2:653161/1‑150 (MQ=255)

                                                                                 ggTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCt                                                                      <  2:1369990/150‑1 (MQ=255)

                                                                                 ggTTTTAGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCt                                                                      >  1:1187357/1‑150 (MQ=255)

                                                                                     ttagTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGaaa                                                                  <  2:4404837/147‑1 (MQ=255)

                                                                                      tagTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAAt                                                                 <  1:2306717/148‑1 (MQ=255)

                                                                                       cGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTTAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATc                                                                <  1:2779088/150‑1 (MQ=255)

                                                                                         tCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAg                                                              >  2:686139/1‑150 (MQ=255)

                                                                                          cTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGc                                                             >  2:24419/1‑150 (MQ=255)

                                                                                          cTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGc                                                             >  2:2815917/1‑150 (MQ=255)

                                                                                            aCCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCga                                                           >  1:1175834/1‑150 (MQ=255)

                                                                                              cAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCgaga                                                         >  1:2501858/1‑150 (MQ=255)

                                                                                              cAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGGTACGCATAATCAATAGCTCCTGAAATCAGCgaga                                                         <  1:1423086/150‑1 (MQ=255)

                                                                                               aGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGaa                                                        >  1:4854015/1‑150 (MQ=255)

                                                                                                      cGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGa                                                 >  1:4524457/1‑150 (MQ=255)

                                                                                                       gTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGAc                                                >  2:3453843/1‑150 (MQ=255)

                                                                                                       gTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGAc                                                >  1:3941420/1‑150 (MQ=255)

                                                                                                        ttATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGAcc                                               <  2:2656651/150‑1 (MQ=255)

                                                                                                        ttATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGAcc                                               <  2:229349/150‑1 (MQ=255)

                                                                                                        ttATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGAcc                                               >  2:505115/1‑150 (MQ=255)

                                                                                                         tataCGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCt                                              >  1:1748465/1‑150 (MQ=255)

                                                                                                            aCGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTcc                                           <  1:1383012/150‑1 (MQ=255)

                                                                                                             cGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAATTCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCca                                          >  2:4689087/1‑150 (MQ=255)

                                                                                                                     aGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGAc                                  >  2:2726815/1‑150 (MQ=255)

                                                                                                                     aGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGAc                                  >  1:1497229/1‑150 (MQ=255)

                                                                                                                      gccgccATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGAca                                 <  1:1400296/150‑1 (MQ=255)

                                                                                                                      gccgccATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGAca                                 <  2:2145450/150‑1 (MQ=255)

                                                                                                                      gccgccATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGAca                                 <  1:4352612/150‑1 (MQ=255)

                                                                                                                       ccgccATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGAcag                                >  1:1090905/1‑150 (MQ=255)

                                                                                                                               aCTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTa                        >  2:194719/1‑150 (MQ=255)

                                                                                                                               aCTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTa                        >  2:30935/1‑150 (MQ=255)

                                                                                                                                    ggctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAg                   >  1:2986414/1‑150 (MQ=255)

                                                                                                                                         tggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCGGGTAGCCAGAacac              >  1:920945/1‑150 (MQ=255)

                                                                                                                                         tggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAacac              >  1:2895234/1‑150 (MQ=255)

                                                                                                                                           gttgttTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACAccc            <  1:2124801/150‑1 (MQ=255)

                                                                                                                                              gttTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAATGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACATTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATc         >  2:258728/1‑150 (MQ=255)

                                                                                                                                                tttCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAg       <  1:3540842/150‑1 (MQ=255)

                                                                                                                                                tttCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAg       >  1:2702201/1‑150 (MQ=255)

                                                                                                                                                 ttCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTACCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAgg      >  2:1041112/1‑150 (MQ=255)

                                                                                                                                                  tCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACTGTATAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAgtc     <  2:3033196/150‑3 (MQ=255)

                                                                                                                                                  tCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGgc     <  1:4783166/150‑1 (MQ=255)

TCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCG  >  CP009273/3860566‑3860864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`