BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,893,385	C→A	7.2%	D185Y (GAT→TAT)	yieL ←	putative xylanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,893,385	0	C	A	7.2%	1178.1 / 43.8	305	D185Y (GAT→TAT)	yieL	putative xylanase
Reads supporting (aligned to +/- strand): ref base C (143/140); new base A (13/9); total (156/149)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 307 total aligned reads displayed.

TGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCG  >  CP009273/3893237‑3893534

tGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCa                                                                                                                                                      >  2:4632123/1‑150 (MQ=255)

  aGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCAcc                                                                                                                                                    >  1:205005/1‑150 (MQ=255)

   ggCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCa                                                                                                                                                   >  2:4408341/1‑150 (MQ=255)

   ggCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCAACGGTATAATTTTCCCTTCAGCAAGCAGGATATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCa                                                                                                                                                   >  2:1702523/1‑150 (MQ=255)

    gCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTAACCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCaa                                                                                                                                                  >  1:4263263/1‑150 (MQ=255)

      aCGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAg                                                                                                                                                >  2:4028504/1‑150 (MQ=255)

      aCGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAg                                                                                                                                                >  2:1575594/1‑150 (MQ=255)

            tCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGa                                                                                                                                          <  1:2232511/150‑1 (MQ=255)

              ttCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATa                                                                                                                                        <  2:4377467/150‑1 (MQ=255)

               tCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAg                                                                                                                                       >  2:1707335/1‑150 (MQ=255)

                    aataatGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATa                                                                                                                                  >  1:2979154/1‑150 (MQ=255)

                     ataatGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATag                                                                                                                                 <  2:1310540/150‑1 (MQ=255)

                     ataatGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATag                                                                                                                                 >  1:3403828/1‑150 (MQ=255)

                      taatGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATaga                                                                                                                                <  2:3509729/150‑1 (MQ=255)

                         tGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCa                                                                                                                             <  1:4898388/150‑1 (MQ=255)

                            ccTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTg                                                                                                                          >  1:4463266/1‑150 (MQ=255)

                               tCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCa                                                                                                                       <  2:529770/150‑1 (MQ=255)

                                  cATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAg                                                                                                                    <  1:4187915/150‑1 (MQ=255)

                                  cATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAg                                                                                                                    <  1:4392003/150‑1 (MQ=255)

                                     cGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCt                                                                                                                 <  1:612546/150‑1 (MQ=255)

                                     cGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCt                                                                                                                 <  1:959232/150‑1 (MQ=255)

                                        tttCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGc                                                                                                              >  2:3890969/1‑150 (MQ=255)

                                         ttCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGcc                                                                                                             >  2:3916529/1‑150 (MQ=255)

                                           cTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCa                                                                                                           <  1:824008/150‑1 (MQ=255)

                                               aTCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGcc                                                                                                       <  1:4797245/150‑1 (MQ=255)

                                                   gggATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGgtg                                                                                                   >  2:4885765/1‑150 (MQ=255)

                                                     gATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTa                                                                                                 <  1:4361638/150‑1 (MQ=255)

                                                     gATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTa                                                                                                 >  1:3858780/1‑150 (MQ=255)

                                                     gATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTa                                                                                                 >  1:371803/1‑150 (MQ=255)

                                                     gATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTa                                                                                                 <  2:3383397/150‑1 (MQ=255)

                                                        caccacCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATcc                                                                                              <  1:3492548/150‑1 (MQ=255)

                                                         accaccAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCt                                                                                             <  2:3615682/150‑1 (MQ=255)

                                                         accaccAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCt                                                                                             <  1:4219001/150‑1 (MQ=255)

                                                         accaccAGCATCGGTTTAATTTGCCCTTCCGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCt                                                                                             <  2:1791610/150‑1 (MQ=255)

                                                          ccaccaGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTg                                                                                            <  1:3754548/150‑1 (MQ=255)

                                                            accaGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGc                                                                                          >  2:1223865/1‑150 (MQ=255)

                                                            accaGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGc                                                                                          >  1:2712309/1‑150 (MQ=255)

                                                              caGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCgg                                                                                        >  1:1282278/1‑150 (MQ=255)

                                                              caGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCgg                                                                                        >  1:863062/1‑150 (MQ=255)

                                                                  aTCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTc                                                                                    >  2:2642221/1‑150 (MQ=255)

                                                                    cGGTTTAATGTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCa                                                                                  <  2:2107244/150‑1 (MQ=255)

                                                                     ggTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAg                                                                                 >  2:3405998/1‑150 (MQ=255)

                                                                      gTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGa                                                                                <  2:3557969/150‑1 (MQ=255)

                                                                       tttAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGac                                                                               <  2:375931/150‑1 (MQ=255)

                                                                       tttAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGac                                                                               >  1:2892198/1‑150 (MQ=255)

                                                                       tttAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGac                                                                               <  1:919995/150‑1 (MQ=255)

                                                                         tAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacat                                                                             >  2:2778566/1‑150 (MQ=255)

                                                                         tAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacat                                                                             <  1:4739099/150‑1 (MQ=255)

                                                                          aaTTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacata                                                                            <  2:677736/150‑1 (MQ=255)

                                                                          aaTTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacata                                                                            >  2:2679269/1‑150 (MQ=255)

                                                                           aTTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacatac                                                                           <  2:4216638/150‑1 (MQ=255)

                                                                           aTTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacatac                                                                           >  1:1528144/1‑150 (MQ=255)

                                                                             tttCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGacatacat                                                                         >  2:4559783/1‑150 (MQ=255)

                                                                                 ccTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGa                                                                     >  1:4981889/1‑150 (MQ=255)

                                                                                   ttCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACg                                                                   >  1:1458155/1‑150 (MQ=255)

                                                                                     cAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGtt                                                                 >  1:3809388/1‑150 (MQ=255)

                                                                                      agcaagcaGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTc                                                                >  2:3817230/1‑150 (MQ=255)

                                                                                       gcaagcaGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCa                                                               >  2:1656042/1‑150 (MQ=255)

                                                                                           gcaGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGAtt                                                           <  1:3293967/150‑1 (MQ=255)

                                                                                            caGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTg                                                          >  1:740785/1‑150 (MQ=255)

                                                                                             aGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGc                                                         <  1:1225684/150‑1 (MQ=255)

                                                                                                 tATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATg                                                     >  2:3805842/1‑150 (MQ=255)

                                                                                                 tATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATg                                                     <  1:4673220/150‑1 (MQ=255)

                                                                                                  aTCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGc                                                    >  2:4294532/1‑150 (MQ=255)

                                                                                                  aTCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGc                                                    >  1:3237555/1‑150 (MQ=255)

                                                                                                     cATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGtt                                                 <  1:1899041/150‑1 (MQ=255)

                                                                                                     cATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGtt                                                 <  1:3283684/150‑1 (MQ=255)

                                                                                                     cATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGtt                                                 >  1:878733/1‑150 (MQ=255)

                                                                                                        gATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGa                                              <  1:4028484/150‑1 (MQ=255)

                                                                                                           ttGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTg                                           >  2:3205277/1‑150 (MQ=255)

                                                                                                            tGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTgg                                          >  1:3099937/1‑150 (MQ=255)

                                                                                                              cGGGATACGCCCCTGATCGAGAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTa                                        <  2:498945/150‑1 (MQ=255)

                                                                                                               gggATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTaa                                       >  2:1855438/1‑150 (MQ=255)

                                                                                                               gggATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTaa                                       <  1:3569301/150‑1 (MQ=255)

                                                                                                                    aCGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTAt                                  <  1:2488396/150‑1 (MQ=255)

                                                                                                                      gCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATgg                                <  1:3259807/150‑1 (MQ=255)

                                                                                                                       ccccTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGc                               <  1:1327729/150‑1 (MQ=255)

                                                                                                                        cccTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCg                              >  2:2513747/1‑150 (MQ=255)

                                                                                                                        cccTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGGGGTAAGTTATGGCg                              >  2:3941526/1‑150 (MQ=255)

                                                                                                                          cTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGAt                            <  1:2443545/150‑1 (MQ=255)

                                                                                                                          cTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGAt                            >  1:2004702/1‑150 (MQ=255)

                                                                                                                           tGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATc                           <  2:568013/150‑1 (MQ=255)

                                                                                                                            gATCGATAGCGGATCGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTTGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCa                          <  2:2239821/150‑1 (MQ=255)

                                                                                                                            gATCGATAGCGGAACGTCCGGTATAACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCa                          <  2:4334479/150‑1 (MQ=255)

                                                                                                                             aTCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCaa                         <  2:3021994/150‑1 (MQ=255)

                                                                                                                              tCGATAGCGGAACGTCCGGTATCACCACAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCaaa                        <  2:4778508/150‑1 (MQ=255)

                                                                                                                                gATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATc                      >  1:2023500/1‑150 (MQ=255)

                                                                                                                                gATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATc                      >  1:1216099/1‑150 (MQ=255)

                                                                                                                                  tAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCAc                    >  2:1759871/1‑150 (MQ=255)

                                                                                                                                   aGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCAcc                   >  2:1297905/1‑150 (MQ=255)

                                                                                                                                       gAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATgc               >  2:2430673/1‑150 (MQ=255)

                                                                                                                                           gTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCaa           >  1:4082558/1‑150 (MQ=255)

                                                                                                                                             ccGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACa         <  2:5097109/150‑1 (MQ=255)

                                                                                                                                              cGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAg        <  2:3121862/150‑1 (MQ=255)

                                                                                                                                              cGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAg        >  1:1619746/1‑150 (MQ=255)

                                                                                                                                                 tATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAAc     >  1:3013566/1‑150 (MQ=255)

                                                                                                                                                 tATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAAc     >  1:4744262/1‑150 (MQ=255)

                                                                                                                                                 tATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAAc     >  2:1618395/1‑150 (MQ=255)

                                                                                                                                                    cACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAAcgcg  >  1:432514/1‑150 (MQ=255)

                                                                                                                                                    cACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAAcgcg  <  1:1707335/150‑1 (MQ=255)

TGAGGCACGAAATCTTCGGGAATAATGCCCTTCGCATCGGTTTCTGTATCCGGGATCACCACCAGCATCGGTTTAATTTTCCCTTCAGCAAGCAGGTTATCCATGATTTGCGGGATACGCCCCTGATCGATAGCGGAACGTCCGGTATCACCAAAGCCGTGATAGAAATAGAGCACTGGCAAAGGCTCGCCCATGCCGGTGTATCCTGGCGGGGTCCAGACATACATCTGACGTTCAGATTGCAATGCGTTGGAGTGGTAAGTTATGGCGATCAAATCACCATGCGCAACAGAACGCG  >  CP009273/3893237‑3893534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`