Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,960,036 G→A 93.2% R87H (CGC→CAC)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,960,0360GA93.2% 1006.8 / 47.9 293R87H (CGC→CAC) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base G (8/12);  new base A (137/136);  total (145/148)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 299 total aligned reads displayed.

TCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACT  >  CP009273/3959889‑3960182
                                                                                                                                                   |                                                                                                                                                  
tCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccacc                                                                                                                                                  >  1:3581810/1‑150 (MQ=255)
 ccTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccaccg                                                                                                                                                 >  1:308797/1‑150 (MQ=255)
 ccTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccaccg                                                                                                                                                 <  2:692710/150‑1 (MQ=255)
     aaGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTc                                                                                                                                             >  1:1110758/1‑150 (MQ=255)
      agcagcACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCa                                                                                                                                            >  1:949423/1‑150 (MQ=255)
       gcagcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCaa                                                                                                                                           >  1:801524/1‑150 (MQ=255)
       gcagcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCaa                                                                                                                                           >  1:1439273/1‑150 (MQ=255)
          gcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCt                                                                                                                                        <  2:808646/150‑1 (MQ=255)
          gcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCt                                                                                                                                        >  1:1033943/1‑150 (MQ=255)
           caCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTc                                                                                                                                       <  2:1019296/150‑1 (MQ=255)
           caCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTc                                                                                                                                       >  1:5092829/1‑150 (MQ=255)
           caCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTc                                                                                                                                       >  1:1311420/1‑150 (MQ=255)
            aCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTcc                                                                                                                                      >  1:4171852/1‑150 (MQ=255)
             cGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCg                                                                                                                                     <  2:3779139/150‑1 (MQ=255)
              gCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGc                                                                                                                                    >  2:1893556/1‑150 (MQ=255)
                aaaGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCAc                                                                                                                                  >  1:4724076/1‑150 (MQ=255)
                aaaGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCAc                                                                                                                                  >  2:3222808/1‑150 (MQ=255)
                  agagTGGCGAAGATATCTTTGGTTATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTg                                                                                                                                >  1:2392219/1‑150 (MQ=255)
                   gagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTgg                                                                                                                               <  1:2141850/150‑1 (MQ=255)
                    agTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGt                                                                                                                              <  2:2021446/150‑1 (MQ=255)
                        gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATa                                                                                                                          <  1:4164853/150‑1 (MQ=255)
                         cGAAGATATCTTTGGTGATGGCGTACTGGATATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATAc                                                                                                                         >  2:4005454/1‑150 (MQ=255)
                         cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATAc                                                                                                                         >  2:4510821/1‑150 (MQ=255)
                          gAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                        <  2:3972582/150‑1 (MQ=255)
                           aaGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCa                                                                                                                       <  1:2888590/150‑1 (MQ=255)
                           aaGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCa                                                                                                                       >  2:851899/1‑150 (MQ=255)
                                  cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTgg                                                                                                                <  2:1439101/150‑1 (MQ=255)
                                  cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTgg                                                                                                                <  2:3196742/150‑1 (MQ=255)
                                  cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTgg                                                                                                                <  2:2747062/150‑1 (MQ=255)
                                   tttGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGt                                                                                                               >  2:787182/1‑150 (MQ=255)
                                    ttGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCGCCTAGCCAAATCCACCGTTTCATCCTCCGCACTGGTGATACCATCTCTGGTa                                                                                                              <  1:3473933/150‑1 (MQ=255)
                                    ttGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTa                                                                                                              >  1:4919726/1‑150 (MQ=255)
                                     tGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTaa                                                                                                             <  1:115478/150‑1 (MQ=255)
                                       gTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGa                                                                                                           <  1:3681796/150‑1 (MQ=255)
                                         gATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt                                                                                                         >  1:5033752/1‑150 (MQ=255)
                                            ggCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgc                                                                                                      <  1:2496228/150‑1 (MQ=255)
                                                tACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgc                                                                                                  <  2:5078867/150‑1 (MQ=255)
                                                tACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgc                                                                                                  >  1:2115086/1‑150 (MQ=255)
                                                 aCTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgcc                                                                                                 <  2:4590353/150‑1 (MQ=255)
                                                  cTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCccgccg                                                                                                <  2:4961573/150‑1 (MQ=255)
                                                   tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGa                                                                                               >  1:924754/1‑150 (MQ=255)
                                                   tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGa                                                                                               >  2:2130134/1‑150 (MQ=255)
                                                    ggAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGaa                                                                                              >  2:2364708/1‑150 (MQ=255)
                                                     gagaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACATCGCCGTTACTGATGACATCTACGTTTCACCTAGCCAAATCCACCGTTTCAACATCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCTCCGaaa                                                                                             >  2:1616287/1‑150 (MQ=255)
                                                         tatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaagaag                                                                                         >  2:3713597/1‑150 (MQ=255)
                                                         tatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaagaag                                                                                         <  1:3696347/150‑1 (MQ=255)
                                                          atTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAgg                                                                                        >  2:3489620/1‑150 (MQ=255)
                                                          atTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAgg                                                                                        <  2:4724076/150‑1 (MQ=255)
                                                             gCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGa                                                                                     >  2:1961679/1‑150 (MQ=255)
                                                              cAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGaa                                                                                    <  1:202584/150‑1 (MQ=255)
                                                               aGGATGGTTTTGGTTTCCTCGGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAAc                                                                                   <  2:3025072/150‑1 (MQ=255)
                                                                ggatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACg                                                                                  <  1:4770496/150‑1 (MQ=255)
                                                                  atggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCt                                                                                >  2:1502767/1‑150 (MQ=255)
                                                                    ggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAt                                                                              <  1:222043/150‑1 (MQ=255)
                                                                     gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTTCCTCGCAGTTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAtt                                                                             <  2:681899/150‑1 (MQ=255)
                                                                     gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAtt                                                                             <  2:2656006/150‑1 (MQ=255)
                                                                       tttGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAtttt                                                                           <  2:340116/150‑1 (MQ=255)
                                                                          ggTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcg                                                                        <  1:1377899/150‑1 (MQ=255)
                                                                           gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                       <  2:129816/150‑1 (MQ=255)
                                                                            tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgct                                                                      <  2:3807909/150‑1 (MQ=255)
                                                                              tcctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgc                                                                    >  2:3431127/1‑150 (MQ=255)
                                                                              tcctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgc                                                                    >  2:4432838/1‑150 (MQ=255)
                                                                              tcctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgc                                                                    <  1:5060892/150‑1 (MQ=255)
                                                                               cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                   <  2:1748478/150‑1 (MQ=255)
                                                                                  ccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaa                                                                >  1:3250791/1‑150 (MQ=255)
                                                                                  ccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaa                                                                >  2:2590402/1‑150 (MQ=255)
                                                                                  ccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaa                                                                <  2:4587408/150‑1 (MQ=255)
                                                                                   cGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                               <  1:4392800/150‑1 (MQ=255)
                                                                                   cGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                               <  2:3597633/150‑1 (MQ=255)
                                                                                    gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAg                                                              >  1:4554750/1‑150 (MQ=255)
                                                                                     ttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                             <  1:3518210/150‑1 (MQ=255)
                                                                                     ttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                             <  1:4120891/150‑1 (MQ=255)
                                                                                          cAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACg                                                        <  1:3340768/150‑1 (MQ=255)
                                                                                          cAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACg                                                        <  1:2387411/150‑1 (MQ=255)
                                                                                             aCAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                                     <  2:4313917/150‑1 (MQ=255)
                                                                                               aGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGtt                                                   <  1:1271702/150‑1 (MQ=255)
                                                                                                gCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTa                                                  <  1:1842978/150‑1 (MQ=255)
                                                                                                   ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACt                                               <  2:2836234/150‑1 (MQ=255)
                                                                                                   ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACt                                               <  2:1033943/150‑1 (MQ=255)
                                                                                                            ccGGTCCTGATGACATCTACGTGTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACGGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAANc                                      <  1:3925952/150‑1 (MQ=255)
                                                                                                               gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                   >  1:2312273/1‑150 (MQ=255)
                                                                                                                tCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGa                                  >  1:3205906/1‑150 (MQ=255)
                                                                                                                tCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGa                                  <  1:3641761/150‑1 (MQ=255)
                                                                                                                 ccTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaa                                 <  1:2255082/150‑1 (MQ=255)
                                                                                                                  cTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaa                                >  2:4108465/1‑150 (MQ=255)
                                                                                                                     atgaCATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                             <  1:1062560/150‑1 (MQ=255)
                                                                                                                       gaCATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc                           >  2:522676/1‑150 (MQ=255)
                                                                                                                             tACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                     >  1:3414281/1‑150 (MQ=255)
                                                                                                                             tACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCAGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                     >  1:5136699/1‑150 (MQ=255)
                                                                                                                                 tttCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa                 >  2:536158/1‑150 (MQ=255)
                                                                                                                                   tCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATAACATCTCTGTTATGATTCGCCCGCCTAAAGATGGTTAACGATTTTTTGCGCTGCTTAAATTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATc               <  1:2364708/150‑1 (MQ=255)
                                                                                                                                      ccTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctc            >  1:2555321/1‑150 (MQ=255)
                                                                                                                                        tAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt          >  1:3645871/1‑150 (MQ=255)
                                                                                                                                           ccAAATCCACCGTTTCAACCTCCGCCCTGGTGATACCATCTCTGGTAAGTTTCGCCCGCCGAAAGATGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGACAACGCCCGCAACAAAATCCTCTTTga       <  1:2204032/150‑1 (MQ=255)
                                                                                                                                            cAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag      >  2:3111558/1‑150 (MQ=255)
                                                                                                                                            cAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag      <  1:1975098/150‑1 (MQ=255)
                                                                                                                                             aaaTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga     >  2:2173071/1‑150 (MQ=255)
                                                                                                                                             aaaTCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga     >  2:2620653/1‑150 (MQ=255)
                                                                                                                                             aaaTCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga     >  1:880975/1‑150 (MQ=255)
                                                                                                                                             aaaTCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga     >  1:2720929/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACT  >  CP009273/3959889‑3960182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑