BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,002,848	G→T	5.4%	V107F (GTT→TTT)	pldB →	lysophospholipase L2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,002,848	0	G	T	5.4%	1419.1 / 34.9	354	V107F (GTT→TTT)	pldB	lysophospholipase L2
Reads supporting (aligned to +/- strand): ref base G (175/160); new base T (12/7); total (187/167)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 355 total aligned reads displayed.

TAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAGGTGTATG  >  CP009273/4002699‑4002996

tAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATg                                                                                                                                                      >  2:4392264/1‑150 (MQ=255)

 aGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGt                                                                                                                                                     <  1:3454394/150‑1 (MQ=255)

   tCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTa                                                                                                                                                   <  2:1870635/150‑1 (MQ=255)

   tCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTa                                                                                                                                                   <  2:626318/150‑1 (MQ=255)

     aTCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGAGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATTTTAAt                                                                                                                                                 <  1:2037908/150‑1 (MQ=255)

      tCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATc                                                                                                                                                >  1:4942431/1‑150 (MQ=255)

        tGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGc                                                                                                                                              >  1:3280529/1‑150 (MQ=255)

         gCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCt                                                                                                                                             <  1:3408581/150‑1 (MQ=255)

           ccGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCttt                                                                                                                                           <  1:1054274/150‑1 (MQ=255)

           ccGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCttt                                                                                                                                           <  2:3860666/150‑1 (MQ=255)

            cGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAAGCGCTTTa                                                                                                                                          >  2:3208558/1‑150 (MQ=255)

             ggggCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTaa                                                                                                                                         <  2:2924820/150‑1 (MQ=255)

             ggggCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTaa                                                                                                                                         >  2:3648776/1‑150 (MQ=255)

              gggCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAAt                                                                                                                                        >  2:2345444/1‑150 (MQ=255)

               ggCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATg                                                                                                                                       >  1:3192247/1‑150 (MQ=255)

               ggCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATg                                                                                                                                       <  2:2179116/150‑1 (MQ=255)

                 cGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGAt                                                                                                                                     >  1:175487/1‑150 (MQ=255)

                 cGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGAt                                                                                                                                     >  1:4016932/1‑150 (MQ=255)

                 cGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGAt                                                                                                                                     >  2:3510174/1‑150 (MQ=255)

                  gTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGAtt                                                                                                                                    <  1:4412440/150‑1 (MQ=255)

                   tATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTa                                                                                                                                   <  2:4740407/150‑1 (MQ=255)

                    aTTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTAt                                                                                                                                  <  1:159659/150‑1 (MQ=255)

                       gagaGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGtt                                                                                                                               >  2:5032659/1‑150 (MQ=255)

                        agagCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTtg                                                                                                                              <  1:4636723/150‑1 (MQ=255)

                         gagCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATTTTAATCGCTTTAATGATTATGTtga                                                                                                                             <  2:1582678/150‑1 (MQ=255)

                         gagCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTCTGTtga                                                                                                                             >  1:4727787/1‑150 (MQ=255)

                         gagCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTtga                                                                                                                             >  1:2740017/1‑150 (MQ=255)

                         gagCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTtga                                                                                                                             >  2:1664353/1‑150 (MQ=255)

                          agCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTtgat                                                                                                                            <  1:1785608/150‑1 (MQ=255)

                            cTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTtgatga                                                                                                                          <  2:1972090/150‑1 (MQ=255)

                               cGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCt                                                                                                                       <  2:1488574/150‑1 (MQ=255)

                                gTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTg                                                                                                                      <  1:1408738/150‑1 (MQ=255)

                                 tAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTgg                                                                                                                     >  1:1087992/1‑150 (MQ=255)

                                  aaaaTATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTggc                                                                                                                    >  2:4023452/1‑150 (MQ=255)

                                  aaaaTATGCGGAACTGGCCTATGACCTGGTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTggc                                                                                                                    <  2:4000/150‑1 (MQ=255)

                                   aaaTATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTggcg                                                                                                                   >  1:4148891/1‑150 (MQ=255)

                                    aaTATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTggcgg                                                                                                                  >  2:4897986/1‑150 (MQ=255)

                                      tatGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCa                                                                                                                >  1:4274085/1‑150 (MQ=255)

                                       atGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCAt                                                                                                               <  2:3319103/150‑1 (MQ=255)

                                          cGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCt                                                                                                            <  1:2904240/150‑1 (MQ=255)

                                          cGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCt                                                                                                            <  2:1713587/150‑1 (MQ=255)

                                          cGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCt                                                                                                            >  1:2084519/1‑150 (MQ=255)

                                           ggAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTg                                                                                                           >  2:223457/1‑150 (MQ=255)

                                             aaCTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGgc                                                                                                         >  1:4437107/1‑150 (MQ=255)

                                              aCTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGgca                                                                                                        >  2:9705/1‑150 (MQ=255)

                                               cTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGgcag                                                                                                       >  2:646566/1‑150 (MQ=255)

                                                tGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGgcagc                                                                                                      >  1:2566430/1‑150 (MQ=255)

                                                 ggCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGgcagca                                                                                                     <  1:5098572/150‑1 (MQ=255)

                                                 ggCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGgcagca                                                                                                     >  1:2180475/1‑150 (MQ=255)

                                                   ccTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCagg                                                                                                   >  2:3365869/1‑150 (MQ=255)

                                                    cTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCagga                                                                                                  <  1:947226/150‑1 (MQ=255)

                                                      aTGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCaggagg                                                                                                >  1:3944542/1‑150 (MQ=255)

                                                       tGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGt                                                                                               >  2:31077/1‑150 (MQ=255)

                                                          ccTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCa                                                                                            <  1:3806087/150‑1 (MQ=255)

                                                            tGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGc                                                                                          >  2:2866007/1‑150 (MQ=255)

                                                            tGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGc                                                                                          <  1:1924221/150‑1 (MQ=255)

                                                            tGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGc                                                                                          >  2:1123288/1‑150 (MQ=255)

                                                              ttCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGccc                                                                                        <  1:2248136/150‑1 (MQ=255)

                                                                 cATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGt                                                                                     >  2:4237910/1‑150 (MQ=255)

                                                                  aTTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTc                                                                                    <  2:1738187/150‑1 (MQ=255)

                                                                  aTTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGAGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTc                                                                                    <  1:1237106/150‑1 (MQ=255)

                                                                   tttGGGGTTTGATGTCTGAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGAGGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTcc                                                                                   <  2:5090742/150‑1 (MQ=255)

                                                                      ggggTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTg                                                                                >  2:4987061/1‑150 (MQ=255)

                                                                         gTTTGCTGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGAGTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGcn                                                                             <  2:1249156/150‑2 (MQ=255)

                                                                          tttGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGt                                                                            >  2:4586892/1‑150 (MQ=255)

                                                                           ttGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTa                                                                           <  2:1302187/150‑1 (MQ=255)

                                                                                 tCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCt                                                                     >  1:2337972/1‑150 (MQ=255)

                                                                                      atcatcGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCtatata                                                                <  2:561106/150‑1 (MQ=255)

                                                                                       tcatcGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATAc                                                               <  2:3752465/150‑1 (MQ=255)

                                                                                        catcGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACt                                                              >  1:4063822/1‑150 (MQ=255)

                                                                                        catcGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACt                                                              >  1:5042295/1‑150 (MQ=255)

                                                                                                 tCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTc                                                     <  1:555347/150‑1 (MQ=255)

                                                                                                  cgcgGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCg                                                    >  1:531793/1‑150 (MQ=255)

                                                                                                  cgcgGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCg                                                    >  1:2677739/1‑150 (MQ=255)

                                                                                                     gggCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATg                                                 >  1:1170703/1‑150 (MQ=255)

                                                                                                        cAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGc                                              >  1:3645197/1‑150 (MQ=255)

                                                                                                           ggACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGt                                           <  2:3163335/150‑1 (MQ=255)

                                                                                                            gACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTg                                          >  1:4538471/1‑150 (MQ=255)

                                                                                                             aCGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGc                                         <  2:4405712/150‑1 (MQ=255)

                                                                                                              cGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCg                                        <  1:3585886/150‑1 (MQ=255)

                                                                                                               gTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGa                                       >  2:1366621/1‑150 (MQ=255)

                                                                                                                   cGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGAtctc                                   >  1:2514107/1‑150 (MQ=255)

                                                                                                                        gCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACat                              >  1:3303434/1‑150 (MQ=255)

                                                                                                                         ccTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACatt                             <  1:2148402/150‑1 (MQ=255)

                                                                                                                            gTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACattatt                          <  2:1910978/150‑1 (MQ=255)

                                                                                                                              tAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTc                        <  2:260626/150‑1 (MQ=255)

                                                                                                                                gCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTg                      <  2:3452434/150‑1 (MQ=255)

                                                                                                                                 ccGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGc                     >  1:4828790/1‑150 (MQ=255)

                                                                                                                                  cGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCa                    <  2:2677739/150‑1 (MQ=255)

                                                                                                                                   gATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCaa                   <  1:164137/150‑1 (MQ=255)

                                                                                                                                   gATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCaa                   >  1:1300095/1‑150 (MQ=255)

                                                                                                                                   gATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCaa                   >  1:5016114/1‑150 (MQ=255)

                                                                                                                                    aTCCGCATCTCGGGCATTTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAAc                  >  2:390972/1‑150 (MQ=255)

                                                                                                                                     tCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACg                 >  2:2466511/1‑150 (MQ=255)

                                                                                                                                             tcGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAg         >  2:2131268/1‑150 (MQ=255)

                                                                                                                                                 gCATTTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAGgtgt     >  2:1790699/1‑150 (MQ=255)

                                                                                                                                                 gCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAGgtgt     <  1:3065500/150‑1 (MQ=255)

                                                                                                                                                  cATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAGGTGTa    <  2:4148880/150‑1 (MQ=255)

                                                                                                                                                  cATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAGGTGTa    >  2:301239/1‑150 (MQ=255)

                                                                                                                                                   aTGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAGGTGTAt   <  2:3917231/150‑1 (MQ=255)

TAGTCATCTGCCCGGGGCGTATTGAGAGCTACGTAAAATATGCGGAACTGGCCTATGACCTGTTCCATTTGGGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGTTAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAGCAGGAGGTTCAGCCCGGTCCGTGGCGTAAACGCTATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAGGTGTATG  >  CP009273/4002699‑4002996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`