BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,024,827	G→T	41.9%	D103Y (GAT→TAT)	pepQ →	proline dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,024,827	0	G	T	41.9%	383.5 / inf	351	D103Y (GAT→TAT)	pepQ	proline dipeptidase
Reads supporting (aligned to +/- strand): ref base G (93/110); new base T (61/87); total (154/197)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

ACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATC  >  CP009273/4024678‑4024976

  ccGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGAt                                                                                                                                                     <  1:2844217/150‑1 (MQ=255)

        ttCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGaa                                                                                                                                               <  2:3228730/150‑1 (MQ=255)

        ttCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGaa                                                                                                                                               <  2:3739929/150‑1 (MQ=255)

         taaaaGCTTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCAGAAACTGTGGTTCTATATGCCGGTTGATTAATGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAg                                                                                                                                              >  2:4624485/3‑150 (MQ=255)

          cAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGt                                                                                                                                             >  1:3430539/1‑150 (MQ=255)

            aaGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGa                                                                                                                                           <  2:4293094/150‑1 (MQ=255)

                 tGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATcgcg                                                                                                                                      >  1:1631681/1‑150 (MQ=255)

                 tGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATcgcg                                                                                                                                      <  2:3140287/150‑1 (MQ=255)

                   ggTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCt                                                                                                                                    <  1:3519544/150‑1 (MQ=255)

                   ggTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCt                                                                                                                                    >  1:827862/1‑150 (MQ=255)

                         ggTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGaa                                                                                                                              <  2:1911855/150‑1 (MQ=255)

                         ggTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGATGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGaa                                                                                                                              >  2:1325580/1‑150 (MQ=255)

                          gTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGGTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGaaa                                                                                                                             >  2:1398908/1‑150 (MQ=255)

                              cTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCg                                                                                                                         <  1:1901570/150‑1 (MQ=255)

                              cTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCg                                                                                                                         <  2:1527124/150‑1 (MQ=255)

                              cTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCg                                                                                                                         <  1:1391182/150‑1 (MQ=255)

                              cTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCg                                                                                                                         >  1:1193471/1‑150 (MQ=255)

                                 aGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATg                                                                                                                      <  1:2207739/150‑1 (MQ=255)

                                  ggTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATgg                                                                                                                     >  2:2365191/1‑150 (MQ=255)

                                   gTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGc                                                                                                                    <  1:4357671/150‑1 (MQ=255)

                                         aaCTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGt                                                                                                              >  2:1234316/1‑150 (MQ=255)

                                         aaCTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGt                                                                                                              <  1:2774360/150‑1 (MQ=255)

                                             gctggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAgcc                                                                                                          <  1:5146288/150‑1 (MQ=255)

                                             gctggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAgcc                                                                                                          >  1:2574508/1‑150 (MQ=255)

                                              ctggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAgcct                                                                                                         <  1:647496/150‑1 (MQ=255)

                                                    gctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAgcctgctgcc                                                                                                   <  2:1481591/150‑1 (MQ=255)

                                                              ggCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTgcgcgc                                                                                         >  1:2990311/1‑150 (MQ=255)

                                                              ggCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTgcgcgc                                                                                         >  1:4434944/1‑150 (MQ=255)

                                                               gCGTGACCCAGCCGCACCTGTGGTTCGATCTGCCGGTTGAGGACTGGCACCCCGGCGAACCGCTGCCGACCTCTGTCTGGACGGAAGAGGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGcgcgcg                                                                                        <  2:3469289/150‑1 (MQ=255)

                                                                  tGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCa                                                                                     <  2:2635334/150‑1 (MQ=255)

                                                                  tGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCa                                                                                     >  1:494625/1‑150 (MQ=255)

                                                                   gAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCaa                                                                                    <  1:2788430/150‑1 (MQ=255)

                                                                    aaCAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAat                                                                                   <  1:3656940/150‑1 (MQ=255)

                                                                      cAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAatat                                                                                 <  2:4922186/150‑1 (MQ=255)

                                                                       aaGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATc                                                                                <  2:2772873/150‑1 (MQ=255)

                                                                       aaGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATc                                                                                <  1:4561157/150‑1 (MQ=255)

                                                                       aaGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATc                                                                                >  1:1342512/1‑150 (MQ=255)

                                                                          ccGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGt                                                                             >  2:837902/1‑150 (MQ=255)

                                                                            gAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTta                                                                           <  1:30438/150‑1 (MQ=255)

                                                                             aaaCTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTtat                                                                          >  1:4591498/1‑150 (MQ=255)

                                                                               aCTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTatat                                                                        <  1:2068834/150‑1 (MQ=255)

                                                                                cTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATc                                                                       >  1:3221089/1‑150 (MQ=255)

                                                                                  gtgGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCgg                                                                     >  2:2102068/1‑150 (MQ=255)

                                                                                  gtgGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCgg                                                                     <  2:1414451/150‑1 (MQ=255)

                                                                                  gtgGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCgg                                                                     >  2:1032477/1‑150 (MQ=255)

                                                                                   tgGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGt                                                                    >  1:1314475/1‑150 (MQ=255)

                                                                                   tgGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGt                                                                    <  2:3948598/150‑1 (MQ=255)

                                                                                     gTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTcc                                                                  <  2:4505447/150‑1 (MQ=255)

                                                                                     gTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTCGCCTGCTGCCTGCTGCGCGCGGCACTATCGGTTATATCGGTcc                                                                  >  2:3197736/1‑150 (MQ=255)

                                                                                     gTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTcc                                                                  <  2:4587072/150‑1 (MQ=255)

                                                                                          aTCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATTGGTTATATCGGTCCGGTGc                                                             >  1:2565977/1‑150 (MQ=255)

                                                                                           tCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGcc                                                            <  1:2446629/150‑1 (MQ=255)

                                                                                            cTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCg                                                           <  1:2731369/150‑1 (MQ=255)

                                                                                               ccGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCACTATCGGTTATATCGGTCCGGTGCCGGac                                                        >  2:4764551/1‑149 (MQ=255)

                                                                                               ccGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGaa                                                        >  1:1412850/1‑150 (MQ=255)

                                                                                               ccGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGaa                                                        >  1:4856952/1‑150 (MQ=255)

                                                                                               ccGGTTGAGTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGAGGTGGAAGTGATCGCGCTGCCGAAAGCCGCTGGCATTGGTAGCCTGCGGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGaa                                                        <  2:572749/150‑1 (MQ=255)

                                                                                                cGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAAc                                                       >  1:4521498/1‑150 (MQ=255)

                                                                                                cGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAAc                                                       <  2:2461135/150‑1 (MQ=255)

                                                                                                  gTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGt                                                     >  2:2252919/1‑150 (MQ=255)

                                                                                                     gATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTgcg                                                  <  2:4366336/150‑1 (MQ=255)

                                                                                                     gATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTgcg                                                  >  1:102733/1‑150 (MQ=255)

                                                                                                      aTTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTgcgc                                                 >  1:4990960/1‑150 (MQ=255)

                                                                                                        tACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTg                                               >  2:2580147/1‑150 (MQ=255)

                                                                                                         aCTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGc                                              <  2:178377/150‑1 (MQ=255)

                                                                                                          cTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCa                                             <  2:3552704/150‑1 (MQ=255)

                                                                                                           tGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCaa                                            <  1:1152134/150‑1 (MQ=255)

                                                                                                             gCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACt                                          <  1:1898435/150‑1 (MQ=255)

                                                                                                              cacaACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTg                                         <  2:2026390/150‑1 (MQ=255)

                                                                                                              cacaACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTg                                         <  1:228403/150‑1 (MQ=255)

                                                                                                                 aaCGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTggga                                      >  2:548405/1‑149 (MQ=255)

                                                                                                                 aaCGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGt                                      <  1:3777778/150‑1 (MQ=255)

                                                                                                                 aaCGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGGAGCCTGCTGCCTGCTGCGCGCGGCAATCTCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGt                                      <  1:1852038/150‑1 (MQ=255)

                                                                                                                   cGTCGAACCGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTAt                                    <  1:289115/150‑1 (MQ=255)

                                                                                                                           cGCTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCa                            <  1:1766892/150‑1 (MQ=255)

                                                                                                                           cGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCa                            <  1:3563779/150‑1 (MQ=255)

                                                                                                                             cTGCCGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGc                          >  1:2473962/1‑150 (MQ=255)

                                                                                                                              tGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCa                         >  2:1707186/1‑150 (MQ=255)

                                                                                                                                 cGACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACa                      <  2:2393121/150‑1 (MQ=255)

                                                                                                                                 cGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCACCTGGGTATTGAGGCCAGCAACa                      >  1:740745/1‑150 (MQ=255)

                                                                                                                                  gACCTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACAt                     <  1:403863/150‑1 (MQ=255)

                                                                                                                                  gACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACAt                     >  1:3825597/1‑150 (MQ=255)

                                                                                                                                   aCCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATc                    <  2:1601968/150‑1 (MQ=255)

                                                                                                                                    ccTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCa                   <  2:2917133/150‑1 (MQ=255)

                                                                                                                                    ccTCTTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCa                   <  1:4280133/150‑1 (MQ=255)

                                                                                                                                     ctctTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTCTTGAGGCCAGCAACATCaa                  >  1:4071667/1‑150 (MQ=255)

                                                                                                                                     ctctTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCaa                  >  1:149846/1‑150 (MQ=255)

                                                                                                                                     ctctTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCaa                  >  1:5117827/1‑150 (MQ=255)

                                                                                                                                      tctTTCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAAc                 <  1:3269516/150‑1 (MQ=255)

                                                                                                                                        tgtCTGGTCTGAATATGGGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCTACATCAAccc               <  2:4200618/148‑1 (MQ=255)

                                                                                                                                         ttCTGGACTGAATATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCg              <  2:2733016/150‑1 (MQ=255)

                                                                                                                                         ttCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCg              <  1:4751568/150‑1 (MQ=255)

                                                                                                                                          tCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGa             <  1:1231248/150‑1 (MQ=255)

                                                                                                                                               aCTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAggg        >  1:3378804/1‑150 (MQ=255)

                                                                                                                                               aCTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAggg        <  1:2675418/150‑1 (MQ=255)

                                                                                                                                               aCTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAggg        >  1:54852/1‑150 (MQ=255)

                                                                                                                                                   aaGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGTACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTa    <  1:4177191/150‑1 (MQ=255)

                                                                                                                                                   aaGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTa    <  1:194315/150‑1 (MQ=255)

                                                                                                                                                   aaGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTa    <  2:4106349/150‑1 (MQ=255)

                                                                                                                                                     gATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATc  <  2:4163703/150‑1 (MQ=255)

ACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATC  >  CP009273/4024678‑4024976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`