BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,107,450	A→G	5.2%	I190T (ATT→ACT)	glpF ←	glycerol facilitator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,107,450	0	A	G	5.2%	988.2 / 33.1	289	I190T (ATT→ACT)	glpF	glycerol facilitator
Reads supporting (aligned to +/- strand): ref base A (130/143); new base G (10/5); total (140/149)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 293 total aligned reads displayed.

GGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGA  >  CP009273/4107301‑4107599

 gCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAAt                                                                                                                                                      >  1:1518785/1‑150 (MQ=255)

     aaaaGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGAcc                                                                                                                                                  >  2:3036791/1‑150 (MQ=255)

      aaaGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCg                                                                                                                                                 >  1:2029467/1‑150 (MQ=255)

       aaGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGc                                                                                                                                                >  2:1229782/1‑150 (MQ=255)

        aGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAGTGACCGCa                                                                                                                                               >  2:1622930/1‑150 (MQ=255)

        aGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCa                                                                                                                                               <  1:4025153/150‑1 (MQ=255)

        aGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCa                                                                                                                                               <  1:1463572/150‑1 (MQ=255)

        aGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCa                                                                                                                                               >  2:1655597/1‑150 (MQ=255)

          cGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAAt                                                                                                                                             >  2:288312/1‑150 (MQ=255)

           ggCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATc                                                                                                                                            <  2:475633/150‑1 (MQ=255)

            gCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCa                                                                                                                                           <  1:3650208/150‑1 (MQ=255)

              aCCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGt                                                                                                                                         >  2:1479066/1‑150 (MQ=255)

               ccAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTa                                                                                                                                        >  2:4289309/1‑150 (MQ=255)

                 aGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGa                                                                                                                                      <  1:4518649/150‑1 (MQ=255)

                 aGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGa                                                                                                                                      <  1:932107/150‑1 (MQ=255)

                  ggAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGAc                                                                                                                                     <  2:2000448/150‑1 (MQ=255)

                  ggAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGAc                                                                                                                                     >  2:1675980/1‑150 (MQ=255)

                    aaGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCa                                                                                                                                   >  2:2112139/1‑150 (MQ=255)

                       tAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATc                                                                                                                                >  2:1101039/1‑150 (MQ=255)

                        aaGGAATGTCTCTGCCGCCGGTAAAGGCGACAGTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCa                                                                                                                               <  2:4734714/150‑1 (MQ=255)

                            aaTGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCaa                                                                                                                           >  2:3432804/1‑150 (MQ=255)

                              tGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAgg                                                                                                                         <  1:2517986/150‑1 (MQ=255)

                               gTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAggg                                                                                                                        >  1:866392/1‑150 (MQ=255)

                                tctcTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGa                                                                                                                       >  2:2741465/1‑150 (MQ=255)

                                 ctctGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAg                                                                                                                      <  2:3854221/150‑1 (MQ=255)

                                   ctGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcc                                                                                                                    <  1:3108593/150‑1 (MQ=255)

                                   ctGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGcc                                                                                                                    >  1:2729960/1‑150 (MQ=255)

                                       cgccgGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAg                                                                                                                >  1:1817093/1‑150 (MQ=255)

                                       cgccgGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAg                                                                                                                >  1:5062948/1‑150 (MQ=255)

                                        gccgGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAgg                                                                                                               >  1:4314278/1‑150 (MQ=255)

                                          cgGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGc                                                                                                             <  2:5104553/150‑1 (MQ=255)

                                           ggTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGcc                                                                                                            <  1:65749/150‑1 (MQ=255)

                                           ggTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGcc                                                                                                            >  2:70197/1‑150 (MQ=255)

                                            gTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCcg                                                                                                           >  2:1772380/1‑150 (MQ=255)

                                             tAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCcgc                                                                                                          >  1:611152/1‑150 (MQ=255)

                                             tAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACAGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCcgc                                                                                                          >  1:167713/1‑150 (MQ=255)

                                               aaGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAGTGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGt                                                                                                        >  1:522050/1‑150 (MQ=255)

                                               aaGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGt                                                                                                        >  2:815969/1‑150 (MQ=255)

                                                   cGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGACACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTa                                                                                                    <  1:4815619/150‑1 (MQ=255)

                                                    gACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTac                                                                                                   <  1:2743597/150‑1 (MQ=255)

                                                     aCATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTaca                                                                                                  >  1:3526646/1‑150 (MQ=255)

                                                        ttGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCg                                                                                               >  2:2457445/1‑150 (MQ=255)

                                                        ttGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCg                                                                                               <  1:2151097/150‑1 (MQ=255)

                                                           ccccAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTg                                                                                            <  2:4229484/150‑1 (MQ=255)

                                                                 cccGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCg                                                                                      <  2:1756391/150‑1 (MQ=255)

                                                                      cagccagGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGt                                                                                 >  1:4316422/1‑150 (MQ=255)

                                                                      cagccagGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGt                                                                                 <  1:3999742/150‑1 (MQ=255)

                                                                       agccagGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGtt                                                                                <  1:1287213/150‑1 (MQ=255)

                                                                         ccagGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTaa                                                                              >  1:2278700/1‑150 (MQ=255)

                                                                           agGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACg                                                                            <  2:9077/150‑1 (MQ=255)

                                                                              cAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCa                                                                         <  1:717625/150‑1 (MQ=255)

                                                                              cAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCa                                                                         <  1:4819548/150‑1 (MQ=255)

                                                                               aaaGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAg                                                                        >  2:4084543/1‑150 (MQ=255)

                                                                                   aCTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATc                                                                    <  1:2244911/150‑1 (MQ=255)

                                                                                     tttCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAg                                                                  <  2:1437163/150‑1 (MQ=255)

                                                                                      ttCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGc                                                                 <  2:3196101/150‑1 (MQ=255)

                                                                                         ggACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGcccc                                                              <  1:700283/150‑1 (MQ=255)

                                                                                         ggACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGcccc                                                              <  1:2652585/150‑1 (MQ=255)

                                                                                           aCCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCTCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCAt                                                            <  2:2305529/150‑1 (MQ=255)

                                                                                            ccGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATc                                                           <  1:4990330/150‑1 (MQ=255)

                                                                                              gAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAAAGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAg                                                         >  1:4210433/1‑150 (MQ=255)

                                                                                                tgTCACGCGCTGGGTTCTTGGCAAAACCTGTCTATGGGCCCTTAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGaa                                                       <  1:2229740/149‑1 (MQ=255)

                                                                                                aGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGac                                                       >  2:5038237/1‑149 (MQ=255)

                                                                                                 gTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAAt                                                      >  1:2342438/1‑150 (MQ=255)

                                                                                                  tCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATa                                                     >  1:2874443/1‑150 (MQ=255)

                                                                                                       cgcTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGt                                                <  2:3349475/150‑1 (MQ=255)

                                                                                                           gggTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATc                                            >  2:4505096/1‑150 (MQ=255)

                                                                                                           gggTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATc                                            <  2:4648659/150‑1 (MQ=255)

                                                                                                            ggTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCa                                           >  1:3028724/1‑150 (MQ=255)

                                                                                                            ggTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCa                                           <  1:4673368/150‑1 (MQ=255)

                                                                                                             gtacATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCat                                          <  2:4153568/147‑2 (MQ=255)

                                                                                                             gTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCAc                                          >  1:1193864/1‑150 (MQ=255)

                                                                                                             gTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCAc                                          >  1:556835/1‑150 (MQ=255)

                                                                                                              ttCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCAcc                                         <  2:1551093/150‑1 (MQ=255)

                                                                                                                 aTGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCAtc                                      >  1:1862915/1‑150 (MQ=255)

                                                                                                                  tGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCAtct                                     >  2:3981650/1‑150 (MQ=255)

                                                                                                                   ggCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCAtctc                                    <  2:4879905/150‑1 (MQ=255)

                                                                                                                   ggCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCAtctc                                    <  2:2710784/150‑1 (MQ=255)

                                                                                                                    gaaaaaCCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCa                                   >  1:1667015/3‑150 (MQ=255)

                                                                                                                    gCAAATCCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCa                                   <  1:344963/150‑1 (MQ=255)

                                                                                                                              tCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAg                         <  2:4950955/150‑1 (MQ=255)

                                                                                                                                aaTGGGCCCATAGATGCGCCACTGAGCGCAATCAGTAGACCAATCAGCTAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCTGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGcc                       <  2:3561179/150‑1 (MQ=255)

                                                                                                                                aaTGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGcc                       >  1:3895122/1‑150 (MQ=255)

                                                                                                                                aaTGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGcc                       >  2:3921228/1‑150 (MQ=255)

                                                                                                                                  tGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTg                     >  2:2344831/1‑150 (MQ=255)

                                                                                                                                   gggCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGc                    >  2:2955589/1‑150 (MQ=255)

                                                                                                                                   gggCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGc                    <  1:4947160/150‑1 (MQ=255)

                                                                                                                                     gCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGcac                  <  2:4195158/150‑1 (MQ=255)

                                                                                                                                     gCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGcac                  <  2:3590212/150‑1 (MQ=255)

                                                                                                                                          tAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAAt             <  1:5129612/150‑1 (MQ=255)

                                                                                                                                           aGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAAtt            <  1:4083377/150‑1 (MQ=255)

                                                                                                                                             aTGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGa          <  1:1017785/150‑1 (MQ=255)

                                                                                                                                             aTGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGa          >  2:326880/1‑150 (MQ=255)

                                                                                                                                              tGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGat         >  2:1453225/1‑150 (MQ=255)

                                                                                                                                              tGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGat         <  2:570630/150‑1 (MQ=255)

                                                                                                                                               gcgcCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGata        >  2:455967/1‑150 (MQ=255)

                                                                                                                                               gcgcCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGata        <  1:2481581/150‑1 (MQ=255)

                                                                                                                                               gcgcCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGata        >  2:1524054/1‑150 (MQ=255)

                                                                                                                                                cgcCAGTGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGatat       >  2:1267279/1‑150 (MQ=255)

                                                                                                                                                 gcCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATg      <  1:2457445/150‑1 (MQ=255)

GGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGA  >  CP009273/4107301‑4107599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`