BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,205,855	A→T	7.8%	A150A (GCA→GCT)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,205,855	0	A	T	7.8%	1074.7 / 63.2	320	A150A (GCA→GCT)	aceB	malate synthase A
Reads supporting (aligned to +/- strand): ref base A (144/151); new base T (13/12); total (157/163)
Fisher's exact test for biased strand distribution p-value = 8.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 324 total aligned reads displayed.

AAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCA  >  CP009273/4205706‑4206004

aaGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCa                                                                                                                                                       <  2:2548501/150‑1 (MQ=255)

   aTGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGc                                                                                                                                                    <  2:2485555/150‑1 (MQ=255)

   aTGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAAACTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGc                                                                                                                                                    >  1:3248075/1‑150 (MQ=255)

     ggTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCaa                                                                                                                                                  <  1:1696406/150‑1 (MQ=255)

       ttatcatcGCGCTCTACGCCAATGTGAAAGTCTTTTTGTCCGATTTCGAAGATTCACTGGCACAAGACTGGATCAAAGTGATCGACGGGCATATTAACCTGCGTGATGCGGTTTTGGGCACCTTCTGTTACTCCACTGAAGCAGGCaaaa                                                                                                                                                <  2:1448768/148‑1 (MQ=255)

        gATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAAt                                                                                                                                               <  1:4916177/150‑1 (MQ=255)

          tCACCGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAAttt                                                                                                                                             <  2:3759444/150‑1 (MQ=255)

               gcgcTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGTCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAg                                                                                                                                        >  2:2706968/1‑150 (MQ=255)

                 gcTCAACGCCAATGTGAAAGTCTTTATGGCCGAGTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGcn                                                                                                                                      <  2:1911646/150‑2 (MQ=255)

                   tCAACGCCAATGTTAATGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCa                                                                                                                                    <  1:1799416/150‑1 (MQ=255)

                      aCGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGc                                                                                                                                 >  1:573298/1‑150 (MQ=255)

                       cGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGcc                                                                                                                                <  1:4734689/150‑1 (MQ=255)

                        gCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGccc                                                                                                                               <  1:4834498/150‑1 (MQ=255)

                         ccAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCa                                                                                                                              >  2:3479701/1‑150 (MQ=255)

                         ccAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCa                                                                                                                              >  2:2795838/1‑150 (MQ=255)

                            aTGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATc                                                                                                                           <  1:196855/150‑1 (MQ=255)

                            aTGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATc                                                                                                                           >  1:1618666/1‑150 (MQ=255)

                              gtgAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCa                                                                                                                         >  1:1841710/1‑150 (MQ=255)

                               tgAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAg                                                                                                                        >  2:2935366/1‑150 (MQ=255)

                                 aaaGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCg                                                                                                                      <  2:4628051/150‑1 (MQ=255)

                                  aaGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCgg                                                                                                                     >  1:3875200/1‑150 (MQ=255)

                                      cTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGtttt                                                                                                                 <  2:1281264/150‑1 (MQ=255)

                                       tttATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTg                                                                                                                <  2:607705/150‑1 (MQ=255)

                                          aTGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGAtt                                                                                                             >  2:3584609/1‑150 (MQ=255)

                                           tGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGAttt                                                                                                            <  1:2988679/150‑1 (MQ=255)

                                             gCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGt                                                                                                          >  2:4054808/1‑150 (MQ=255)

                                               cGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCg                                                                                                        <  2:4323521/150‑1 (MQ=255)

                                                  tttCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGt                                                                                                     <  1:3602894/150‑1 (MQ=255)

                                                     cGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTAcg                                                                                                  <  2:3904999/150‑1 (MQ=255)

                                                     cGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTAcg                                                                                                  >  1:31484/1‑150 (MQ=255)

                                                     cGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTAcg                                                                                                  >  2:4197741/1‑150 (MQ=255)

                                                      gAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTAcgc                                                                                                 >  2:5007721/1‑150 (MQ=255)

                                                      gAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTAcgc                                                                                                 >  2:57050/1‑150 (MQ=255)

                                                      gAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTCcgc                                                                                                 >  2:4993474/1‑150 (MQ=255)

                                                      gAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTAcgc                                                                                                 >  2:3557409/1‑150 (MQ=255)

                                                      gAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTAcgc                                                                                                 <  2:1749886/150‑1 (MQ=255)

                                                       aaGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTAcgcg                                                                                                <  1:3795988/150‑1 (MQ=255)

                                                        aGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCgg                                                                                               >  1:4297078/1‑150 (MQ=255)

                                                         gATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGt                                                                                              <  2:2175705/150‑1 (MQ=255)

                                                          aTTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTc                                                                                             <  2:2021096/150‑1 (MQ=255)

                                                           ttCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGGCt                                                                                            >  2:1185040/1‑150 (MQ=255)

                                                               cTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCAc                                                                                        >  1:4375394/1‑150 (MQ=255)

                                                               cTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCAc                                                                                        <  1:3964041/150‑1 (MQ=255)

                                                               cTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGAGTTGTCGGGTACGCGGTCTGCAc                                                                                        >  2:776492/1‑150 (MQ=255)

                                                                tGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACt                                                                                       <  2:4711110/150‑1 (MQ=255)

                                                                 ggCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACtt                                                                                      <  1:841122/150‑1 (MQ=255)

                                                                 ggCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACtt                                                                                      <  2:1244359/150‑1 (MQ=255)

                                                                  gCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACAATCAGTTACACCAATGAAGAAGGCAAAATTTACAAGCTCAAGCCCAATCAAGCGGTTTTGATTTGTCGGGTAAGCGGTCTGCACTTg                                                                                     >  1:2425838/1‑150 (MQ=255)

                                                                     ccAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCg                                                                                  >  1:1097300/1‑150 (MQ=255)

                                                                      cAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCgg                                                                                 <  1:136307/150‑1 (MQ=255)

                                                                        gACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGaa                                                                               >  2:663679/1‑150 (MQ=255)

                                                                        gACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGaa                                                                               <  1:2583938/150‑1 (MQ=255)

                                                                         aCTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGaaa                                                                              <  2:3229336/150‑1 (MQ=255)

                                                                         aCTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGaaa                                                                              <  2:4893457/150‑1 (MQ=255)

                                                                         aCTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGaaa                                                                              <  1:3137459/150‑1 (MQ=255)

                                                                           tGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGaaaaa                                                                            <  1:2203435/150‑1 (MQ=255)

                                                                             gAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACa                                                                          <  2:4104571/150‑1 (MQ=255)

                                                                              aaCAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACAt                                                                         >  2:1822176/1‑150 (MQ=255)

                                                                               aCAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATg                                                                        >  1:954783/1‑150 (MQ=255)

                                                                               aCAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATg                                                                        >  2:2881011/1‑150 (MQ=255)

                                                                                cAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGt                                                                       >  2:327536/1‑150 (MQ=255)

                                                                                       aTCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTgg                                                                >  1:1529713/1‑150 (MQ=255)

                                                                                         cGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCg                                                              <  2:2664750/150‑1 (MQ=255)

                                                                                         cGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCg                                                              <  2:1764497/150‑1 (MQ=255)

                                                                                             gggCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCgtggt                                                          >  1:3323656/1‑150 (MQ=255)

                                                                                                  aaTTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGc                                                     >  1:4958642/1‑150 (MQ=255)

                                                                                                  aaTTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGc                                                     >  1:2846376/1‑150 (MQ=255)

                                                                                                     tAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAAt                                                  >  1:5033497/1‑150 (MQ=255)

                                                                                                     tAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAAt                                                  >  1:2136566/1‑150 (MQ=255)

                                                                                                     tAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAAt                                                  >  1:677076/1‑150 (MQ=255)

                                                                                                      aaCCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATc                                                 >  1:1244946/1‑150 (MQ=255)

                                                                                                         cTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATcccc                                              <  1:4689099/150‑1 (MQ=255)

                                                                                                         cTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATcccc                                              <  1:5050109/150‑1 (MQ=255)

                                                                                                         cTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATcccc                                              >  1:642036/1‑150 (MQ=255)

                                                                                                          tGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCACTCCCCg                                             >  2:1328550/1‑150 (MQ=255)

                                                                                                          tGCGTGATGCGGTTAACGGCAACATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCATCGGTTTTGATTTGTCGGGTACGCGGTCTTCACTTGCCGGTAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCg                                             <  1:4403026/150‑1 (MQ=255)

                                                                                                           gCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCTGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCgg                                            <  2:1676686/150‑1 (MQ=255)

                                                                                                             gTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCa                                          >  1:760367/1‑150 (MQ=255)

                                                                                                                 tGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCt                                      <  1:2699550/150‑1 (MQ=255)

                                                                                                                     gTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGttt                                  <  2:2693208/150‑1 (MQ=255)

                                                                                                                      ttAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTg                                 >  2:4516937/1‑150 (MQ=255)

                                                                                                                       tAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGa                                >  1:1028584/1‑150 (MQ=255)

                                                                                                                        aaCGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGAt                               <  2:163121/150‑1 (MQ=255)

                                                                                                                          cGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGAttt                             <  1:1197338/150‑1 (MQ=255)

                                                                                                                              aCCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTgcg                         >  1:1315464/1‑150 (MQ=255)

                                                                                                                                 aTCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGctc                      >  1:4030636/1‑150 (MQ=255)

                                                                                                                                      ttACACCAAGGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTAttt                 <  2:3185798/150‑1 (MQ=255)

                                                                                                                                       tACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATttc                <  2:4900183/150‑1 (MQ=255)

                                                                                                                                        acacCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATttct               <  1:921722/150‑1 (MQ=255)

                                                                                                                                        acacCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATttct               <  1:1791959/150‑1 (MQ=255)

                                                                                                                                         cacCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATttctt              <  1:2199192/150‑1 (MQ=255)

                                                                                                                                           ccAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTcc            <  2:4727362/150‑1 (MQ=255)

                                                                                                                                             aaTGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCcac          >  1:3100290/1‑150 (MQ=255)

                                                                                                                                             aaTGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCcac          <  1:3693961/150‑1 (MQ=255)

                                                                                                                                              aTGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCcaca         <  2:1618666/150‑1 (MQ=255)

                                                                                                                                              aTGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCcaca         <  2:1162387/150‑1 (MQ=255)

                                                                                                                                                gAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAAc       <  2:1097300/150‑1 (MQ=255)

                                                                                                                                                 aaGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACt      >  2:1943695/1‑150 (MQ=255)

                                                                                                                                                  aGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTa     >  2:359755/1‑150 (MQ=255)

                                                                                                                                                     aGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCa  <  2:1021979/150‑1 (MQ=255)

AAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCA  >  CP009273/4205706‑4206004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`