Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,220,948 G→T 14.7% D223Y (GAT→TAT)  rluF → 23S rRNA pseudouridine(2604) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,220,9480GT14.7% 984.3 / 105.8 330D223Y (GAT→TAT) rluF23S rRNA pseudouridine(2604) synthase
Reads supporting (aligned to +/- strand):  ref base G (128/152);  new base T (18/32);  total (146/184)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 344 total aligned reads displayed.

CGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGC  >  CP009273/4220799‑4221097
                                                                                                                                                     |                                                                                                                                                     
cgcCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGcgcg                                                                                                                                                       >  2:215327/1‑150 (MQ=255)
   cGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGAtt                                                                                                                                                    <  2:4448512/150‑1 (MQ=255)
    gTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGAttt                                                                                                                                                   <  2:4769565/150‑1 (MQ=255)
     tttGTCTTCCGCATTACCCTTGTGCTGGTGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATTAAGTGAAAAAGCTGGAACGCACGCGCTTCATGTACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTa                                                                                                                                                  <  1:5061735/150‑1 (MQ=255)
          cTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACcga                                                                                                                                             <  1:888162/150‑1 (MQ=255)
          cTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACcga                                                                                                                                             <  1:3841007/150‑1 (MQ=255)
           ttCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACcgac                                                                                                                                            >  2:1305359/1‑150 (MQ=255)
              cGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGAt                                                                                                                                         >  1:675796/1‑150 (MQ=255)
               gCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATg                                                                                                                                        >  1:1820814/1‑150 (MQ=255)
               gCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATg                                                                                                                                        >  2:720408/1‑150 (MQ=255)
                cagtACCCTGGGGCAGGGGCTGAACCGTCAGCTCCGGCGCTTGTGCGAGCCTTTCGGCTATGACGGGAAAAAGCTGGACCGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGTAATGGCGCGATTTAACCGACGATGa                                                                                                                                       <  2:4142529/147‑1 (MQ=255)
                  ttACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGt                                                                                                                                     >  2:3344510/1‑150 (MQ=255)
                   tACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGtt                                                                                                                                    <  2:612383/150‑1 (MQ=255)
                   tACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGtt                                                                                                                                    <  2:3807438/150‑1 (MQ=255)
                     cccTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTaa                                                                                                                                  <  1:3261840/150‑1 (MQ=255)
                       cTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATc                                                                                                                                >  1:1434604/1‑150 (MQ=255)
                        tGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCg                                                                                                                               <  1:3943941/150‑1 (MQ=255)
                           tGCTGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGATGTTAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGATTGGCGGGATTTAACCGACGATGAGTTAATCGAcc                                                                                                                            <  1:514386/150‑1 (MQ=255)
                           tGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGACTGGCGCTATTTAACCGACGATGAGTTAATCGAcc                                                                                                                            >  1:3498058/1‑150 (MQ=255)
                           tGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGAcc                                                                                                                            <  1:301072/150‑1 (MQ=255)
                            gCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACct                                                                                                                           <  2:240771/150‑1 (MQ=255)
                             cAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACctc                                                                                                                          >  2:3068567/1‑150 (MQ=255)
                              aGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACctct                                                                                                                         <  1:4969688/150‑1 (MQ=255)
                                gggCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCttt                                                                                                                       >  1:3596898/1‑150 (MQ=255)
                                  gCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTaa                                                                                                                     <  2:1365946/150‑1 (MQ=255)
                                  gCTGAACCGGCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTaa                                                                                                                     <  1:4474429/150‑1 (MQ=255)
                                      acccGTCAGTTCCGGCGCTTGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGACCGTTCGCTTAAGCGGCTTTCCGGTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTc                                                                                                                 <  1:15334/148‑1 (MQ=255)
                                           tCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGa                                                                                                            <  2:1573956/150‑1 (MQ=255)
                                           tCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGa                                                                                                            >  1:3341468/1‑150 (MQ=255)
                                              gATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGaaaa                                                                                                         <  2:1128639/150‑1 (MQ=255)
                                                  cGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTcc                                                                                                     >  2:2740460/1‑150 (MQ=255)
                                                   ggCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAATCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCct                                                                                                    >  1:643301/1‑150 (MQ=255)
                                                   ggCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCct                                                                                                    >  1:1660299/1‑150 (MQ=255)
                                                   ggCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCct                                                                                                    >  1:2270471/1‑150 (MQ=255)
                                                     cgcATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCctct                                                                                                  <  1:4940840/150‑1 (MQ=255)
                                                      gcATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCtt                                                                                                 <  2:4739462/150‑1 (MQ=255)
                                                      gcATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCtt                                                                                                 <  2:4590152/150‑1 (MQ=255)
                                                        aTGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTcc                                                                                               <  2:2031036/150‑1 (MQ=255)
                                                          gtgCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGa                                                                                             >  2:2274148/1‑150 (MQ=255)
                                                           tgCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAg                                                                                            <  1:977881/150‑1 (MQ=255)
                                                             cGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGt                                                                                          <  1:4284897/150‑1 (MQ=255)
                                                             cGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGt                                                                                          >  2:1423303/1‑150 (MQ=255)
                                                                       ggCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTaaa                                                                                >  2:4672033/1‑150 (MQ=255)
                                                                         cTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGc                                                                              <  2:1594720/150‑1 (MQ=255)
                                                                          tATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCg                                                                             >  1:4627571/1‑150 (MQ=255)
                                                                            tGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTTGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGaa                                                                           <  1:1061184/150‑1 (MQ=255)
                                                                              aaGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAgg                                                                         <  1:792324/150‑1 (MQ=255)
                                                                              aaGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAgg                                                                         >  1:4918218/1‑150 (MQ=255)
                                                                                 tGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCa                                                                      >  2:4269868/1‑150 (MQ=255)
                                                                                     aaaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAAcc                                                                  <  1:3668125/150‑1 (MQ=255)
                                                                                      aaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCg                                                                 >  2:4016476/1‑150 (MQ=255)
                                                                                         cTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGaaa                                                              <  2:859555/150‑1 (MQ=255)
                                                                                         cTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGaaa                                                              <  1:4569584/150‑1 (MQ=255)
                                                                                          tGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGACTGGCGCTATTTAACCGACGATGAGTTACTCGACCTCTTTAAGCTCAGTGAAAATTCCTCTTCCGAGGTACAACCTCAAGCGAAGGCCAAACCGcaaa                                                             >  2:5004124/1‑150 (MQ=255)
                                                                                          tGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGaaaa                                                             <  1:1853691/150‑1 (MQ=255)
                                                                                           ggAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAAc                                                            <  2:2655591/150‑1 (MQ=255)
                                                                                           ggAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCCAAAGCGACGGCCAAACCGAAAAc                                                            >  2:4935926/1‑150 (MQ=255)
                                                                                            gAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACa                                                           >  2:261131/1‑150 (MQ=255)
                                                                                              acgcacgcGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGc                                                         <  1:2421037/150‑1 (MQ=255)
                                                                                                 cacgcGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCggg                                                      <  1:246408/150‑1 (MQ=255)
                                                                                                     cgcATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATc                                                  <  1:4729700/150‑1 (MQ=255)
                                                                                                       catcatGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCaa                                                >  1:4079369/1‑150 (MQ=255)
                                                                                                         tcatGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAAc                                              <  2:684383/150‑1 (MQ=255)
                                                                                                          catGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACg                                             >  1:4209841/1‑150 (MQ=255)
                                                                                                           atGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGt                                            <  2:792637/150‑1 (MQ=255)
                                                                                                                cGTTAGCTTAAGCGGCTTTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGt                                       <  1:3761839/150‑1 (MQ=255)
                                                                                                                cGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCCTCAAACGTCCAGt                                       >  1:2788378/1‑150 (MQ=255)
                                                                                                                 gTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTc                                      >  1:2223580/1‑150 (MQ=255)
                                                                                                                    aGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGtt                                   <  2:3838466/150‑1 (MQ=255)
                                                                                                                     gCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTCAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTa                                  >  2:4213845/1‑150 (MQ=255)
                                                                                                                         atgCGGCTTTCCGCTGGGGGAATGGCGCGTTTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCTAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGAt                              <  1:1337802/148‑1 (MQ=255)
                                                                                                                          aGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATg                             <  1:1298943/150‑1 (MQ=255)
                                                                                                                          aGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATg                             >  2:1638240/1‑150 (MQ=255)
                                                                                                                            cGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGAGGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGa                           >  2:1916979/1‑150 (MQ=255)
                                                                                                                             ggCATTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaa                          >  1:1214236/1‑150 (MQ=255)
                                                                                                                             ggCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaa                          >  1:4795221/1‑150 (MQ=255)
                                                                                                                             ggCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaa                          >  1:2653189/1‑150 (MQ=255)
                                                                                                                               cATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaaaa                        >  1:291911/1‑150 (MQ=255)
                                                                                                                                aTTCCGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaaaaa                       >  1:2906401/1‑150 (MQ=255)
                                                                                                                                  tCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAc                     >  2:2571132/1‑150 (MQ=255)
                                                                                                                                   ccGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcg                    >  1:3762549/1‑150 (MQ=255)
                                                                                                                                    cGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcgg                   >  1:1076405/1‑150 (MQ=255)
                                                                                                                                    cGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcgg                   >  1:3115190/1‑150 (MQ=255)
                                                                                                                                    cGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTGAAGATGGAAAAAAcgg                   >  2:2891365/1‑150 (MQ=255)
                                                                                                                                      cTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcggcg                 <  1:257206/150‑1 (MQ=255)
                                                                                                                                       tGGGGGAAGGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcggcgg                <  1:1421706/150‑1 (MQ=255)
                                                                                                                                        gggggAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGa               <  1:1639682/150‑1 (MQ=255)
                                                                                                                                         ggggAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGaa              <  2:2938898/150‑1 (MQ=255)
                                                                                                                                         ggggAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGaa              >  2:231488/1‑150 (MQ=255)
                                                                                                                                           ggAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGaaaa            <  2:2224598/150‑1 (MQ=255)
                                                                                                                                             aaTGGCGCTATTTAACCGACTATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAg          <  2:3252931/150‑1 (MQ=255)
                                                                                                                                             aaTGGCGCTATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAg          <  2:2533655/150‑1 (MQ=255)
                                                                                                                                             aaTGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAg          <  2:4821226/150‑1 (MQ=255)
                                                                                                                                             aaTGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAg          <  2:646561/150‑1 (MQ=255)
                                                                                                                                               tGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGc        <  2:1395403/150‑1 (MQ=255)
                                                                                                                                               tGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGc        <  2:1713843/150‑1 (MQ=255)
                                                                                                                                                  cgcgATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGACAAAGGCGGt     >  2:3705277/1‑150 (MQ=255)
                                                                                                                                                     gATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGc  >  1:4407259/1‑150 (MQ=255)
                                                                                                                                                     gATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGc  >  2:3438729/1‑150 (MQ=255)
                                                                                                                                                     gATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGc  >  2:1095204/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGC  >  CP009273/4220799‑4221097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑