Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,274,926 A→C 94.8% I72M (ATT→ATG)  yjcH ← DUF485 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,274,9260AC94.8% 1291.9 / 30.3 323I72M (ATT→ATG) yjcHDUF485 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (9/8);  new base C (157/149);  total (166/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 325 total aligned reads displayed.

GTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTG  >  CP009273/4274780‑4275075
                                                                                                                                                  |                                                                                                                                                     
gTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATc                                                                                                                                                    <  2:813291/150‑1 (MQ=255)
gTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATc                                                                                                                                                    >  1:1592643/1‑150 (MQ=255)
   aGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACt                                                                                                                                                 <  1:4276875/150‑1 (MQ=255)
   aGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACt                                                                                                                                                 <  1:3969462/150‑1 (MQ=255)
     cTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTcc                                                                                                                                               <  2:4805528/150‑1 (MQ=255)
      tGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCa                                                                                                                                              >  1:581338/1‑150 (MQ=255)
       gCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCaa                                                                                                                                             <  2:3728611/150‑1 (MQ=255)
        ctaaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAac                                                                                                                                            >  1:806029/3‑150 (MQ=255)
        cGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAac                                                                                                                                            >  2:2392055/1‑150 (MQ=255)
          aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAacac                                                                                                                                          >  1:744984/1‑150 (MQ=255)
                aGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTg                                                                                                                                    <  1:4704584/150‑1 (MQ=255)
                  tgtgGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGa                                                                                                                                  <  2:3998009/150‑1 (MQ=255)
                    tgGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAAt                                                                                                                                <  2:1269066/150‑1 (MQ=255)
                    tgGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAAt                                                                                                                                >  2:1597219/1‑150 (MQ=255)
                     ggcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATa                                                                                                                               <  2:1805510/150‑1 (MQ=255)
                      gcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATAc                                                                                                                              <  1:2479353/150‑1 (MQ=255)
                       cggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATAcc                                                                                                                             <  2:3006439/150‑1 (MQ=255)
                       cggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATAcc                                                                                                                             >  1:5086265/1‑150 (MQ=255)
                       cggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATAcc                                                                                                                             >  2:663343/1‑150 (MQ=255)
                         gcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACcgc                                                                                                                           >  2:4419425/1‑150 (MQ=255)
                         gcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACcgc                                                                                                                           >  1:227607/1‑150 (MQ=255)
                           aaGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCgt                                                                                                                         >  1:3021575/1‑150 (MQ=255)
                           aaGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCgt                                                                                                                         <  1:2129847/150‑1 (MQ=255)
                               gcCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTTGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGa                                                                                                                     <  1:4284319/150‑1 (MQ=255)
                                 cGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACg                                                                                                                   >  2:1682209/1‑150 (MQ=255)
                                  gTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGc                                                                                                                  >  2:1199178/1‑150 (MQ=255)
                                    cAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTg                                                                                                                >  2:4278038/1‑150 (MQ=255)
                                        aCTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtgt                                                                                                            <  2:1068281/150‑1 (MQ=255)
                                        aCTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGgtgt                                                                                                            >  2:2817923/1‑150 (MQ=255)
                                         ctctTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGtt                                                                                                           <  1:286516/150‑1 (MQ=255)
                                          tctTTTCATGATGCCTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTc                                                                                                          <  1:1589248/150‑1 (MQ=255)
                                              ttCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGAt                                                                                                      >  2:2700211/1‑150 (MQ=255)
                                                cATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTc                                                                                                    >  1:884500/1‑150 (MQ=255)
                                                 atgatgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCa                                                                                                   >  2:2291911/1‑150 (MQ=255)
                                                   gatgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgc                                                                                                 >  2:3098636/1‑150 (MQ=255)
                                                    atgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcg                                                                                                <  1:605756/150‑1 (MQ=255)
                                                    atgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcg                                                                                                >  2:1989237/1‑150 (MQ=255)
                                                    atgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcg                                                                                                >  2:15403/1‑150 (MQ=255)
                                                      gCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcggc                                                                                              >  2:3379666/1‑150 (MQ=255)
                                                      gCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcggc                                                                                              >  2:4655418/1‑150 (MQ=255)
                                                      gCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAgcggc                                                                                              >  2:4673052/1‑150 (MQ=255)
                                                         tGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTg                                                                                           >  1:1985871/1‑150 (MQ=255)
                                                           tACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGcc                                                                                         >  2:3229165/1‑150 (MQ=255)
                                                            aCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGccc                                                                                        <  1:2051489/150‑1 (MQ=255)
                                                                  tGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCccagccag                                                                                  <  1:4442817/150‑1 (MQ=255)
                                                                    cAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCagccagcc                                                                                >  2:2931687/1‑150 (MQ=255)
                                                                    cAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCagccagcc                                                                                <  2:2257899/150‑1 (MQ=255)
                                                                    cAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCagccagcc                                                                                >  2:4970985/1‑150 (MQ=255)
                                                                     aGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCg                                                                               >  1:144789/1‑150 (MQ=255)
                                                                       gaattCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCggg                                                                             <  2:5099521/147‑1 (MQ=255)
                                                                       gACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCggg                                                                             >  2:2163912/1‑150 (MQ=255)
                                                                         cTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGgcg                                                                           >  1:3720811/1‑150 (MQ=255)
                                                                           tCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGcgcg                                                                         <  1:331580/150‑1 (MQ=255)
                                                                             attattAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGaa                                                                       <  1:4643311/150‑1 (MQ=255)
                                                                              ttattaAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAg                                                                      <  1:2603068/150‑1 (MQ=255)
                                                                                  taAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGa                                                                  <  1:1030935/150‑1 (MQ=255)
                                                                                     gACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCa                                                               <  1:3468650/150‑1 (MQ=255)
                                                                                     gACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCa                                                               <  1:1926611/150‑1 (MQ=255)
                                                                                     gACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAGACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATGGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCa                                                               <  2:1094700/150‑1 (MQ=255)
                                                                                      aCGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAg                                                              >  1:2694313/1‑150 (MQ=255)
                                                                                       cGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGt                                                             <  1:853508/150‑1 (MQ=255)
                                                                                        ggTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTa                                                            >  2:1509990/1‑150 (MQ=255)
                                                                                        ggTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTa                                                            >  1:4846917/1‑150 (MQ=255)
                                                                                        ggTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTa                                                            >  2:2336050/1‑150 (MQ=255)
                                                                                         gTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTaa                                                           <  2:2512005/150‑1 (MQ=255)
                                                                                           cGAATTCGTCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTaaaa                                                         <  2:3743628/150‑1 (MQ=255)
                                                                                            gAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAAt                                                        >  2:324660/1‑150 (MQ=255)
                                                                                             aaTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATa                                                       >  1:5112558/1‑150 (MQ=255)
                                                                                             aaTTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATa                                                       <  1:301096/150‑1 (MQ=255)
                                                                                                  gCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGcc                                                  <  2:1015940/150‑1 (MQ=255)
                                                                                                    cGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGa                                                >  2:328010/1‑150 (MQ=255)
                                                                                                    cGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGa                                                >  1:4053910/1‑150 (MQ=255)
                                                                                                    cGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGa                                                <  2:4256371/150‑1 (MQ=255)
                                                                                                     gTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGat                                               <  2:54455/150‑1 (MQ=255)
                                                                                                     gTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGat                                               >  1:1492663/1‑150 (MQ=255)
                                                                                                     gTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGat                                               >  2:4305055/1‑150 (MQ=255)
                                                                                                       tCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGAGCAGTAAAATAAAGCCGatat                                             >  1:3641435/1‑150 (MQ=255)
                                                                                                        cgcccgccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGAtata                                            >  1:4219549/1‑150 (MQ=255)
                                                                                                             gccAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTg                                       <  2:416567/150‑1 (MQ=255)
                                                                                                               cAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGcc                                     >  1:1830321/1‑150 (MQ=255)
                                                                                                               cAGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGcc                                     >  2:453577/1‑150 (MQ=255)
                                                                                                                aGATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCa                                    <  1:3655558/150‑1 (MQ=255)
                                                                                                                 gATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGACGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAg                                   >  1:2952933/1‑150 (MQ=255)
                                                                                                                 gATGTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAg                                   >  2:2665649/1‑150 (MQ=255)
                                                                                                                    gTAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCat                                <  1:3767110/150‑1 (MQ=255)
                                                                                                                     tAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCata                               <  2:3614349/150‑1 (MQ=255)
                                                                                                                     tAGATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCata                               <  1:4955157/150‑1 (MQ=255)
                                                                                                                       gATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataat                             <  2:3455534/150‑1 (MQ=255)
                                                                                                                       gATACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCataat                             >  2:2065574/1‑150 (MQ=255)
                                                                                                                         tACCGGTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCAtaataa                           >  1:2391935/1‑150 (MQ=255)
                                                                                                                             ggTGAGAACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGa                       <  2:697292/150‑1 (MQ=255)
                                                                                                                                 agaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAgg                   >  2:3867305/1‑150 (MQ=255)
                                                                                                                                  gaACAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGa                  <  2:2466959/150‑1 (MQ=255)
                                                                                                                                    aCAAAGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtg                <  2:581338/150‑1 (MQ=255)
                                                                                                                                      aaaGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt              >  1:1190982/1‑150 (MQ=255)
                                                                                                                                      aaaGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggt              <  1:3081359/150‑1 (MQ=255)
                                                                                                                                       aaGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggtg             <  2:953876/150‑1 (MQ=255)
                                                                                                                                        aGGAGATCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGAtggtgg            <  1:1322895/150‑1 (MQ=255)
                                                                                                                                             aTCACCATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAAc       <  2:3571280/150‑1 (MQ=255)
                                                                                                                                                 caATCACTCCAACCCCAATTGGAATCCCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGtt   <  1:1215899/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTG  >  CP009273/4274780‑4275075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑