Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,290,080 G→T 100% I256I (ATC→ATA)  mdtP ← outer membrane factor of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,290,0800GT96.0% 992.0 / 27.8 298I256I (ATC→ATA) mdtPouter membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base G (4/8);  new base T (147/139);  total (151/147)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 300 total aligned reads displayed.

TGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAA  >  CP009273/4289934‑4290229
                                                                                                                                                  |                                                                                                                                                     
 gCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAttt                                                                                                                                                   <  2:3718164/150‑1 (MQ=255)
 gCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAttt                                                                                                                                                   <  1:2176830/150‑1 (MQ=255)
 gCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAttt                                                                                                                                                   <  2:2623800/150‑1 (MQ=255)
   aGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGc                                                                                                                                                 <  1:4047142/150‑1 (MQ=255)
     aTCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGTTTCTCGCGTTTCGGTTATTTGccc                                                                                                                                               >  2:1610479/1‑150 (MQ=255)
     aTCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGccc                                                                                                                                               <  2:876997/150‑1 (MQ=255)
      tCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCt                                                                                                                                              <  2:4439786/150‑1 (MQ=255)
      tCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCt                                                                                                                                              <  1:1223883/150‑1 (MQ=255)
      tCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCt                                                                                                                                              >  2:2736336/1‑150 (MQ=255)
       ccGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCtt                                                                                                                                             <  1:1287319/150‑1 (MQ=255)
       ccGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCtt                                                                                                                                             <  1:4919931/150‑1 (MQ=255)
       ccGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCtt                                                                                                                                             >  1:1191720/1‑150 (MQ=255)
        cGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCttt                                                                                                                                            <  1:3589804/150‑1 (MQ=255)
        cGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCttt                                                                                                                                            <  1:2951532/150‑1 (MQ=255)
         gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                           <  2:4169826/150‑1 (MQ=255)
         gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                           >  2:2101722/1‑150 (MQ=255)
            cGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACg                                                                                                                                        >  2:3882602/1‑150 (MQ=255)
             gTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACgg                                                                                                                                       >  2:3117442/1‑150 (MQ=255)
                  gCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaaca                                                                                                                                  <  2:746707/150‑1 (MQ=255)
                     aGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAAtt                                                                                                                               <  1:1182773/150‑1 (MQ=255)
                      gCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAAttt                                                                                                                              <  2:3980080/150‑1 (MQ=255)
                       cAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTg                                                                                                                             <  1:4983889/150‑1 (MQ=255)
                       cAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTg                                                                                                                             <  1:4873142/150‑1 (MQ=255)
                       cAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTg                                                                                                                             <  2:2882897/150‑1 (MQ=255)
                       cAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGGTTCGGTTATTTGCCCTTTGACGGCAACAATTTg                                                                                                                             >  1:1897145/1‑150 (MQ=255)
                          cTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGttt                                                                                                                          <  2:2525442/150‑1 (MQ=255)
                           tCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTACTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCCATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTa                                                                                                                         <  1:5011110/150‑1 (MQ=255)
                           tCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTa                                                                                                                         <  2:3128444/150‑1 (MQ=255)
                            cATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTAt                                                                                                                        >  1:3316709/1‑150 (MQ=255)
                               aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGa                                                                                                                     <  2:307993/150‑1 (MQ=255)
                               aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGa                                                                                                                     >  1:3949079/1‑150 (MQ=255)
                               aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGa                                                                                                                     >  2:362174/1‑150 (MQ=255)
                               aaGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGa                                                                                                                     <  2:1895733/150‑1 (MQ=255)
                                agagagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGAc                                                                                                                    >  2:3122531/1‑150 (MQ=255)
                                agagagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGAc                                                                                                                    >  2:1762292/1‑150 (MQ=255)
                                agagagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGAc                                                                                                                    >  1:2562356/1‑150 (MQ=255)
                                agagagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGAc                                                                                                                    <  1:1973668/150‑1 (MQ=255)
                                   gagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGc                                                                                                                 <  1:1046708/150‑1 (MQ=255)
                                       gtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGa                                                                                                             <  1:2588222/150‑1 (MQ=255)
                                            cGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtg                                                                                                        >  1:2100136/1‑150 (MQ=255)
                                             ggAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgt                                                                                                       >  1:4763933/1‑150 (MQ=255)
                                             ggAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgt                                                                                                       <  2:2453037/150‑1 (MQ=255)
                                             ggAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGATGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgt                                                                                                       >  2:77389/1‑150 (MQ=255)
                                                aTGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGcc                                                                                                    <  2:1398695/150‑1 (MQ=255)
                                                aTGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGcc                                                                                                    >  1:1579331/1‑150 (MQ=255)
                                                      gTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcgcg                                                                                              <  1:3424797/150‑1 (MQ=255)
                                                          ggACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGt                                                                                          >  2:2190529/1‑150 (MQ=255)
                                                           gACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTg                                                                                         <  1:1268637/150‑1 (MQ=255)
                                                              ttGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGaa                                                                                      <  2:3301641/150‑1 (MQ=255)
                                                              ttGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGaa                                                                                      <  2:4161405/150‑1 (MQ=255)
                                                              ttGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGaa                                                                                      <  2:1955437/150‑1 (MQ=255)
                                                               tGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTAGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGaaa                                                                                     >  2:3862037/1‑150 (MQ=255)
                                                                  ggTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGc                                                                                  >  1:879374/1‑150 (MQ=255)
                                                                       tGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                                                             <  2:3357178/150‑1 (MQ=255)
                                                                         ccACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgcg                                                                           >  1:296192/1‑150 (MQ=255)
                                                                         ccACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgcg                                                                           >  1:1475116/1‑150 (MQ=255)
                                                                          cACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTgcgc                                                                          >  1:3667328/1‑150 (MQ=255)
                                                                           aCCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCt                                                                         <  2:2954407/150‑1 (MQ=255)
                                                                           aCCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCt                                                                         <  2:12899/150‑1 (MQ=255)
                                                                             cGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTc                                                                       <  2:702557/150‑1 (MQ=255)
                                                                               gTCTGATCTCCGGCATATCGCTGCCGCCCGCGCCACTCACTGCACGCAGAGCTTCTCGCGTTTCGTTTATTTGCCCTTCGACGGCAACTATTTGTTTATCGACCGCCAGCATCTGGGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCa                                                                     <  2:5139371/150‑1 (MQ=255)
                                                                                tCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAg                                                                    <  1:2491256/150‑1 (MQ=255)
                                                                                tCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAg                                                                    <  1:3122531/150‑1 (MQ=255)
                                                                                     tctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGt                                                               >  2:4508977/1‑150 (MQ=255)
                                                                                      ctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTg                                                              <  1:456165/150‑1 (MQ=255)
                                                                                      ctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGGTTCGGGTATTTGCCCGTTGACGGCAACAATTTGTGTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGCAAGGCACTTGCGCTTCCAGACCGTg                                                              >  2:137640/1‑150 (MQ=255)
                                                                                       tcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgc                                                             <  2:939193/150‑1 (MQ=255)
                                                                                       tcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgc                                                             <  1:4365282/150‑1 (MQ=255)
                                                                                        ccGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcg                                                            >  1:4435310/1‑150 (MQ=255)
                                                                                         cGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcgc                                                           <  2:5024684/150‑1 (MQ=255)
                                                                                          ggCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGcc                                                          >  2:844329/1‑150 (MQ=255)
                                                                                            cATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCAc                                                        >  2:3168592/1‑150 (MQ=255)
                                                                                               atCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACttt                                                     <  1:2088645/150‑1 (MQ=255)
                                                                                                  gCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTAct                                                  <  2:664710/150‑1 (MQ=255)
                                                                                                   cTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActc                                                 >  2:1499672/1‑150 (MQ=255)
                                                                                                   cTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActc                                                 <  1:5477/150‑1 (MQ=255)
                                                                                                         cccGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTgc                                           <  2:3698169/150‑1 (MQ=255)
                                                                                                         cccGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTgc                                           >  1:5116840/1‑150 (MQ=255)
                                                                                                             cgcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCtt                                       >  1:3823198/1‑150 (MQ=255)
                                                                                                             cgcCAATCAATGCACGCAGAGATACTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATGTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCtt                                       >  1:1143415/1‑150 (MQ=255)
                                                                                                              gcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCttt                                      <  1:3343559/150‑1 (MQ=255)
                                                                                                                  atcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCAcc                                  <  1:4587298/150‑1 (MQ=255)
                                                                                                                      atGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGt                              >  1:1936216/1‑150 (MQ=255)
                                                                                                                       tGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTa                             <  2:821138/150‑1 (MQ=255)
                                                                                                                         cACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaat                           <  2:4692226/150‑1 (MQ=255)
                                                                                                                            gCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaa                        <  2:3271319/150‑1 (MQ=255)
                                                                                                                             cAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaat                       <  1:717393/150‑1 (MQ=255)
                                                                                                                             cAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaat                       >  1:4096523/1‑150 (MQ=255)
                                                                                                                              agagATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaatc                      >  2:4567280/1‑150 (MQ=255)
                                                                                                                                agaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATcac                    >  1:2760756/1‑150 (MQ=255)
                                                                                                                                agaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGAGGAAAGTCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATcac                    >  1:2269250/1‑150 (MQ=255)
                                                                                                                                  aTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACCCTGGTGCGCTTTCACCGCGTAATCAATCACAt                  <  2:543787/150‑1 (MQ=255)
                                                                                                                                  aTTATCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACAt                  >  2:4898659/1‑150 (MQ=255)
                                                                                                                                   ttCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATc                 >  1:1692985/1‑150 (MQ=255)
                                                                                                                                         cgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt           <  2:4809897/150‑1 (MQ=255)
                                                                                                                                         cgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt           <  1:797658/150‑1 (MQ=255)
                                                                                                                                          gTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGtt          >  2:2287345/1‑150 (MQ=255)
                                                                                                                                          gTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGtt          <  2:3369470/150‑1 (MQ=255)
                                                                                                                                           tttCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGttt         <  2:1191720/150‑1 (MQ=255)
                                                                                                                                                  gATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTaa  >  2:1119372/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTCTAA  >  CP009273/4289934‑4290229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑