Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,398,152 A→T 8.9% Q561L (CAG→CTG)  rnr → exoribonuclease R, RNase R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,398,1520AT 8.9% 1228.4 / 85.9 368Q561L (CAG→CTG) rnrexoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base A (169/166);  new base T (22/11);  total (191/177)
Fisher's exact test for biased strand distribution p-value = 9.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 373 total aligned reads displayed.

TAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGC  >  CP009273/4398003‑4398301
                                                                                                                                                     |                                                                                                                                                     
 aaCAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAg                                                                                                                                                      <  1:3498027/150‑1 (MQ=255)
    aaGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTcc                                                                                                                                                   >  1:3025925/1‑150 (MQ=255)
      gCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTa                                                                                                                                                 <  1:1633414/150‑1 (MQ=255)
       ccGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTAt                                                                                                                                                <  1:4715098/150‑1 (MQ=255)
         ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTTCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTAttc                                                                                                                                              >  2:4996303/1‑148 (MQ=255)
           aaCCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCTGTCCTATgcgc                                                                                                                                            >  2:2460612/1‑150 (MQ=255)
           aaCCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATgcgc                                                                                                                                            >  2:3508997/1‑150 (MQ=255)
            aCCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCa                                                                                                                                           >  1:1795439/1‑150 (MQ=255)
              cgcgTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCCTTGCTGTCCTATGCGCACt                                                                                                                                         >  1:1780749/1‑150 (MQ=255)
                   gACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACt                                                                                                                                    <  1:4862469/150‑1 (MQ=255)
                    aCTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACtt                                                                                                                                   <  2:4595364/150‑1 (MQ=255)
                     cTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTc                                                                                                                                  <  1:1293155/150‑1 (MQ=255)
                       aCGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCTGTCCTATGCGCACATTACTTCGc                                                                                                                                >  1:1207362/1‑150 (MQ=255)
                       aCGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGc                                                                                                                                <  2:2423494/150‑1 (MQ=255)
                        cgcgGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGcc                                                                                                                               <  2:787641/150‑1 (MQ=255)
                            gAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGAtt                                                                                                                           <  2:1743186/150‑1 (MQ=255)
                              gctgctGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATtcg                                                                                                                         <  2:4259353/150‑1 (MQ=255)
                              gctgctGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATtcg                                                                                                                         <  1:429029/150‑1 (MQ=255)
                               ctgctgGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATtcgt                                                                                                                        <  2:3365494/150‑1 (MQ=255)
                                  ctgGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTcgtcgt                                                                                                                     <  1:2711141/150‑1 (MQ=255)
                                    ggAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTa                                                                                                                   >  1:850682/1‑150 (MQ=255)
                                         cGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCTGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAg                                                                                                              <  1:960942/150‑1 (MQ=255)
                                           gTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGAc                                                                                                            >  1:1915433/1‑150 (MQ=255)
                                           gTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGAc                                                                                                            <  1:2238004/150‑1 (MQ=255)
                                           gTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGAc                                                                                                            >  1:4164548/1‑150 (MQ=255)
                                                cGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGAc                                                                                                       >  2:4328283/1‑150 (MQ=255)
                                                  aTCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGc                                                                                                     >  1:894130/1‑150 (MQ=255)
                                                     gTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGc                                                                                                  <  2:1609868/150‑1 (MQ=255)
                                                      tCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCCGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCa                                                                                                 >  2:1007125/1‑150 (MQ=255)
                                                      tCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCa                                                                                                 >  1:722800/1‑150 (MQ=255)
                                                      tCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCa                                                                                                 >  1:2911403/1‑150 (MQ=255)
                                                        cTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCAcc                                                                                               <  1:640054/150‑1 (MQ=255)
                                                        cTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCAcc                                                                                               >  2:1480081/1‑150 (MQ=255)
                                                        cTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCAcc                                                                                               >  2:1689109/1‑150 (MQ=255)
                                                        cTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCAcc                                                                                               <  2:1997058/150‑1 (MQ=255)
                                                        cTGATGCAGAAATGCCGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCAcc                                                                                               <  2:2555500/150‑1 (MQ=255)
                                                         tGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACcg                                                                                              >  1:1469220/1‑150 (MQ=255)
                                                           aTGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACcgcg                                                                                            >  2:3651143/1‑150 (MQ=255)
                                                           aTGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACcgcg                                                                                            <  2:3441137/150‑1 (MQ=255)
                                                           aTGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACcgcg                                                                                            <  2:3220121/150‑1 (MQ=255)
                                                            tGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCgcgc                                                                                           >  1:4518917/1‑150 (MQ=255)
                                                             gCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGcc                                                                                          <  1:3854160/150‑1 (MQ=255)
                                                               aGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCAt                                                                                        <  1:266029/150‑1 (MQ=255)
                                                                gAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCTGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCAtt                                                                                       >  1:1391316/1‑150 (MQ=255)
                                                                 aaaTGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTa                                                                                      >  2:4865400/1‑150 (MQ=255)
                                                                      ctgcAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCTGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAatat                                                                                 <  1:2287782/150‑1 (MQ=255)
                                                                        gcAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATct                                                                               >  2:2689412/1‑150 (MQ=255)
                                                                         cAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATctg                                                                              <  1:692787/150‑1 (MQ=255)
                                                                          aaaCCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATctgc                                                                             >  2:57533/1‑150 (MQ=255)
                                                                          aaaCCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATctgc                                                                             >  1:2471532/1‑150 (MQ=255)
                                                                           aaCCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATctgct                                                                            >  2:3850061/1‑150 (MQ=255)
                                                                            aCCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATctgctg                                                                           <  1:3088631/150‑1 (MQ=255)
                                                                            aCCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATctgctg                                                                           <  1:1871132/150‑1 (MQ=255)
                                                                             ccATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTgg                                                                          >  1:1697073/1‑150 (MQ=255)
                                                                              cATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGc                                                                         <  2:5086600/150‑1 (MQ=255)
                                                                               aTGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCg                                                                        <  1:4616740/150‑1 (MQ=255)
                                                                                  ctgctgcGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGaaa                                                                     <  1:3835246/150‑1 (MQ=255)
                                                                                   tgctgcGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAag                                                                    >  2:112817/1‑150 (MQ=255)
                                                                                      tgcGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGc                                                                 >  1:3625031/1‑150 (MQ=255)
                                                                                      tgcGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGc                                                                 >  2:3150455/1‑150 (MQ=255)
                                                                                             gATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCa                                                          <  2:2913490/150‑1 (MQ=255)
                                                                                             gATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCa                                                          >  1:2046076/1‑150 (MQ=255)
                                                                                             gATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCCTCGTTATCCAGACCAGACGCTGCACCGCGCCAGTAAATATCTGCTGGCGAAAGAGCAGGGGCa                                                          >  1:5124873/1‑150 (MQ=255)
                                                                                              aTGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCAt                                                         <  1:1060645/150‑1 (MQ=255)
                                                                                              aTGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCAt                                                         >  1:425147/1‑150 (MQ=255)
                                                                                              aTGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCAt                                                         <  1:1260676/150‑1 (MQ=255)
                                                                                                gAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCTGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCa                                                       >  2:3025952/1‑150 (MQ=255)
                                                                                                gAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCa                                                       <  1:4347550/150‑1 (MQ=255)
                                                                                                gAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCa                                                       >  1:2367973/1‑150 (MQ=255)
                                                                                                gAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCa                                                       >  1:586723/1‑150 (MQ=255)
                                                                                                 aaaCAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAg                                                      <  1:5016275/150‑1 (MQ=255)
                                                                                                    cAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGc                                                   <  2:3996833/150‑1 (MQ=255)
                                                                                                        cGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAaca                                               <  2:3074831/150‑1 (MQ=255)
                                                                                                        cGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAaca                                               <  2:3925816/150‑1 (MQ=255)
                                                                                                          aTTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAAcacc                                             >  1:5020934/1‑150 (MQ=255)
                                                                                                           tttACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCTGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAAcacca                                            <  2:1284008/150‑1 (MQ=255)
                                                                                                           tttACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAAcacca                                            <  1:4066333/150‑1 (MQ=255)
                                                                                                           tttACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAAcacca                                            >  2:658466/1‑150 (MQ=255)
                                                                                                             tACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACt                                          <  1:2569197/150‑1 (MQ=255)
                                                                                                                gATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCTGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGaa                                       <  2:1860663/150‑1 (MQ=255)
                                                                                                                 aTCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGaaa                                      >  2:2773631/1‑150 (MQ=255)
                                                                                                                     aGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACcgg                                  <  1:159182/150‑1 (MQ=255)
                                                                                                                      gAAACCCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACcggc                                 <  2:4634370/150‑1 (MQ=255)
                                                                                                                             gTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTAcc                          <  2:2237266/150‑1 (MQ=255)
                                                                                                                             gTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTAcc                          >  1:151427/1‑150 (MQ=255)
                                                                                                                             gTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTAcc                          >  2:300110/1‑150 (MQ=255)
                                                                                                                             gTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACGTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTAcc                          <  2:2839394/150‑1 (MQ=255)
                                                                                                                               ggTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCat                        <  1:3331177/150‑1 (MQ=255)
                                                                                                                                  cACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCattat                     <  2:4430784/150‑1 (MQ=255)
                                                                                                                                  cACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCattat                     <  1:1961658/150‑1 (MQ=255)
                                                                                                                                         gCCTGGCATTGCTGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATgg              >  2:2122964/1‑150 (MQ=255)
                                                                                                                                            tGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAag           >  1:1083160/1‑150 (MQ=255)
                                                                                                                                            tGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAag           <  1:1080224/150‑1 (MQ=255)
                                                                                                                                                aTTGCTGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGAt       >  1:3464680/1‑150 (MQ=255)
                                                                                                                                                 ttGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGAtg      >  2:2607710/1‑150 (MQ=255)
                                                                                                                                                  tGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGAtgc     >  2:453320/1‑150 (MQ=255)
                                                                                                                                                  tGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGAtgc     <  2:192998/150‑1 (MQ=255)
                                                                                                                                                   gCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGAtgct    <  1:3741242/150‑1 (MQ=255)
                                                                                                                                                    cAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGAtgctg   >  2:3979012/1‑150 (MQ=255)
                                                                                                                                                     aGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGACGAGAtgctgc  >  2:4509008/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAAAACCGTGGTCACTTTGGCCTGGCATTGCAGTCCTATGCGCACTTTACTTCGCCGATTCGTCGTTATCCAGACCTGACGCTGCACCGCGCCATTAAATATCTGCTGGCGAAAGAGCAGGGGCATCAGGGCAACACCACTGAAACCGGCGGCTACCATTATTCGATGGAAGAGATGCTGC  >  CP009273/4398003‑4398301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑