BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,427,761	G→T	9.0%	A80S (GCC→TCC)	ytfI →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,427,761	0	G	T	9.0%	1097.1 / 55.6	306	A80S (GCC→TCC)	ytfI	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (132/145); new base T (11/18); total (143/163)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

TTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTT  >  CP009273/4427612‑4427910

   ttAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCa                                                                                                                                                    >  2:2345920/1‑150 (MQ=255)

    tAACAACATCCATCATGCATTTAAGAAAAAAAATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCAt                                                                                                                                                   <  1:1413415/150‑1 (MQ=255)

     aacaacATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATg                                                                                                                                                  >  1:3213965/1‑150 (MQ=255)

      acaacaTCCATCATGCATTTAAGAAAAAAAATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATgg                                                                                                                                                 <  1:4312203/150‑1 (MQ=255)

          catccatcATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATaa                                                                                                                                             >  1:2022795/1‑150 (MQ=255)

          catccatcATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATaa                                                                                                                                             <  2:4379517/150‑1 (MQ=255)

          catccatcATGCATTTAAGAAAAAAAATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATaa                                                                                                                                             >  2:2893241/1‑150 (MQ=255)

           atccatcATGCATTTAAGAAAAAAAATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAg                                                                                                                                            >  2:1810973/1‑150 (MQ=255)

           atccatcATGCATTTAAGAAAAAAAATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAg                                                                                                                                            <  1:4623975/150‑1 (MQ=255)

            tccatcATGCATTTAAGAAAAAAAATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGt                                                                                                                                           >  2:1252211/1‑150 (MQ=255)

               atcatTCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTATATTTGAGGGAGTTATTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTc                                                                                                                                        <  1:524531/150‑1 (MQ=255)

                tcatGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCt                                                                                                                                       <  1:1757586/150‑1 (MQ=255)

                  atGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTa                                                                                                                                     >  2:581534/1‑150 (MQ=255)

                     cATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCa                                                                                                                                  <  2:4618949/150‑1 (MQ=255)

                     cATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCa                                                                                                                                  <  1:2715103/150‑1 (MQ=255)

                      aTTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAg                                                                                                                                 >  2:4753619/1‑150 (MQ=255)

                       tttAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGt                                                                                                                                >  1:3363372/1‑150 (MQ=255)

                       tttAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATCGATAAGTTTCTTATCAGt                                                                                                                                >  1:1151289/1‑150 (MQ=255)

                          aaGAAAAAAAATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTc                                                                                                                             >  1:3454913/1‑150 (MQ=255)

                               aaaaGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACTCCATGGATAAGTTTCTTATCAGTTTCAGCCt                                                                                                                        <  1:1042820/150‑1 (MQ=255)

                               aaaaGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCt                                                                                                                        >  2:2862591/1‑150 (MQ=255)

                                aaaaATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCtt                                                                                                                       >  1:1169303/1‑150 (MQ=255)

                                   gATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTaaa                                                                                                                    <  1:3597204/150‑1 (MQ=255)

                                       ggATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATa                                                                                                                <  1:5026226/150‑1 (MQ=255)

                                       ggATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATa                                                                                                                >  2:1917643/1‑150 (MQ=255)

                                          tttCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACg                                                                                                             <  2:4558368/150‑1 (MQ=255)

                                           ttCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACTCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACgg                                                                                                            <  1:483863/150‑1 (MQ=255)

                                             cGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACTCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTg                                                                                                          <  1:1332629/150‑1 (MQ=255)

                                             cGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTg                                                                                                          <  2:254387/150‑1 (MQ=255)

                                              gAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGc                                                                                                         <  1:4925886/150‑1 (MQ=255)

                                               aaTTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGcc                                                                                                        >  2:1121106/1‑150 (MQ=255)

                                                aTTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCg                                                                                                       <  1:2101200/150‑1 (MQ=255)

                                                   aTGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTACAGATAACGGTGCCGAat                                                                                                    <  2:4806112/150‑1 (MQ=255)

                                                    tGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAata                                                                                                   >  2:1383831/1‑150 (MQ=255)

                                                    tGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAata                                                                                                   <  2:4918487/150‑1 (MQ=255)

                                                     gATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAatat                                                                                                  <  1:401823/150‑1 (MQ=255)

                                                      aTAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAAtata                                                                                                 <  2:431869/150‑1 (MQ=255)

                                                       tAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACTCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATAc                                                                                                >  2:2262926/1‑150 (MQ=255)

                                                       tAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATAc                                                                                                >  1:1655523/1‑150 (MQ=255)

                                                        aGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACa                                                                                               >  1:3992442/1‑150 (MQ=255)

                                                          ccTGTTATCTTTGAGGGAGTTCTTTCGGTGCGCACCTCAGATTGATAACAGTGGGTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACTCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATACCGGTGCCGAATATACAAt                                                                                             <  2:3051969/150‑1 (MQ=255)

                                                            tGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGa                                                                                           >  1:3843218/1‑150 (MQ=255)

                                                                 aCTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTg                                                                                      <  1:2228762/150‑1 (MQ=255)

                                                                  cTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGc                                                                                     <  1:3194240/150‑1 (MQ=255)

                                                                  cTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGc                                                                                     <  2:4677933/150‑1 (MQ=255)

                                                                  cTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGc                                                                                     <  1:1305545/150‑1 (MQ=255)

                                                                  cTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATCGc                                                                                     <  1:1382057/150‑1 (MQ=255)

                                                                   tttGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCg                                                                                    <  2:3826609/150‑1 (MQ=255)

                                                                      gAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAgg                                                                                 >  1:369933/1‑150 (MQ=255)

                                                                       aGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACAATGCGAGGt                                                                                >  2:2295228/1‑150 (MQ=255)

                                                                          gAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGt                                                                             <  1:2341901/150‑1 (MQ=255)

                                                                           aGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTg                                                                            >  1:2915616/1‑150 (MQ=255)

                                                                               atttCGTTGCGCAACTCAGATTGATAAAAGTGGTTATAAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGAtt                                                                        <  2:4109991/149‑1 (MQ=255)

                                                                                     ttGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATa                                                                  <  2:3520861/150‑1 (MQ=255)

                                                                                      tGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATAc                                                                 <  2:1492787/150‑1 (MQ=255)

                                                                                      tGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATAc                                                                 >  2:496595/1‑150 (MQ=255)

                                                                                         gcAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACgaa                                                              <  2:1608567/150‑1 (MQ=255)

                                                                                          cAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACTCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACgaag                                                             <  2:373543/150‑1 (MQ=255)

                                                                                          cAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACgaag                                                             <  2:3847264/150‑1 (MQ=255)

                                                                                           aaCTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACgaaga                                                            <  2:2022795/150‑1 (MQ=255)

                                                                                             cTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGaaa                                                          <  2:445428/150‑1 (MQ=255)

                                                                                             cTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGaaa                                                          <  1:3177471/150‑1 (MQ=255)

                                                                                              tCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAAt                                                         >  1:44266/1‑150 (MQ=255)

                                                                                                aGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACTCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAc                                                       >  1:4407651/1‑150 (MQ=255)

                                                                                                aGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAc                                                       >  2:1829884/1‑150 (MQ=255)

                                                                                                aGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAc                                                       <  1:4059101/150‑1 (MQ=255)

                                                                                                 gATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAcc                                                      >  1:4977538/1‑150 (MQ=255)

                                                                                                 gATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAcc                                                      >  1:2957499/1‑150 (MQ=255)

                                                                                                  aTTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAccc                                                     <  2:2608955/150‑1 (MQ=255)

                                                                                                  aTTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAccc                                                     <  1:3727222/150‑1 (MQ=255)

                                                                                                  aTTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAccc                                                     <  2:2459295/150‑1 (MQ=255)

                                                                                                  aTTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAccc                                                     >  2:4071168/1‑150 (MQ=255)

                                                                                                      aTAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATaa                                                 >  1:278965/1‑150 (MQ=255)

                                                                                                      aTAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATaa                                                 >  1:2215272/1‑150 (MQ=255)

                                                                                                      aTAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATaa                                                 >  1:2215169/1‑150 (MQ=255)

                                                                                                        aaaaGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACa                                               >  1:4235144/1‑150 (MQ=255)

                                                                                                        aaaaGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACa                                               <  1:1685627/150‑1 (MQ=255)

                                                                                                          aaGTGG‑TATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATa                                            <  2:3963235/150‑1 (MQ=255)

                                                                                                           aGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATa                                            >  1:185195/1‑150 (MQ=255)

                                                                                                             tGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATaaa                                          <  1:859706/150‑1 (MQ=255)

                                                                                                             tGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATaaa                                          <  2:763410/150‑1 (MQ=255)

                                                                                                              ggTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACTCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAAt                                         >  2:810207/1‑150 (MQ=255)

                                                                                                              ggTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAAt                                         >  2:1251307/1‑150 (MQ=255)

                                                                                                                  aTCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAat                                     >  2:904213/1‑150 (MQ=255)

                                                                                                                   tCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAata                                    <  2:1001251/150‑1 (MQ=255)

                                                                                                                   tCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAata                                    >  1:5095375/1‑150 (MQ=255)

                                                                                                                     aGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAAtcta                                  <  2:3712639/150‑1 (MQ=255)

                                                                                                                          cTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACtca                             >  2:2671808/1‑150 (MQ=255)

                                                                                                                             tcatcaAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACtcattc                          <  1:2588294/150‑1 (MQ=255)

                                                                                                                              catcaAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACtcattca                         >  2:3163211/1‑150 (MQ=255)

                                                                                                                                 caAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATgg                      >  1:4784430/1‑150 (MQ=255)

                                                                                                                                   aaaaaTGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGAt                    >  1:1318925/1‑150 (MQ=255)

                                                                                                                                     aaaTGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGAttt                  <  2:5128262/150‑1 (MQ=255)

                                                                                                                                      aaTGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTc                 >  2:4914086/1‑150 (MQ=255)

                                                                                                                                            aCTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTG‑ATACCAATAAATAACCATAACCAAAAATGAATAGAACTCATTAAAGGGTTTCAAAAAcc          >  2:2983035/1‑150 (MQ=255)

                                                                                                                                             cTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAAcc          >  1:247828/1‑150 (MQ=255)

                                                                                                                                               gTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGt        >  2:1693394/1‑150 (MQ=255)

                                                                                                                                                  cACTCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGaa     <  2:4270244/150‑1 (MQ=255)

                                                                                                                                                  cACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACTGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTTAATACGAAGAAATAACCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAAAAGTGaa     >  1:157281/1‑150 (MQ=255)

                                                                                                                                                  cACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGaa     >  1:393434/1‑150 (MQ=255)

TTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTT  >  CP009273/4427612‑4427910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`