BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,428,092	G→T	44.5%	G190V (GGA→GTA)	ytfI →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,428,092	0	G	T	44.5%	270.2 / inf	326	G190V (GGA→GTA)	ytfI	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (86/95); new base T (81/64); total (167/159)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 337 total aligned reads displayed.

GATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGA  >  CP009273/4427944‑4428239

gATATTCTACAGAAAAAACAGTCTGACATACCAAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATGGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACAagga                                                                                                                                                    >  1:2704597/1‑150 (MQ=255)

 atatTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCagtag                                                                                                                                                   <  1:4709675/150‑1 (MQ=255)

 atatTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCaggag                                                                                                                                                   >  1:162264/1‑150 (MQ=255)

  tatTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCagtagg                                                                                                                                                  >  2:3042918/1‑150 (MQ=255)

     tCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTgg                                                                                                                                               >  2:2972044/1‑150 (MQ=255)

      cTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGa                                                                                                                                              >  1:448385/1‑150 (MQ=255)

       tACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGaa                                                                                                                                             >  2:4749113/1‑150 (MQ=255)

       tACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAGTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGaa                                                                                                                                             <  1:4303929/150‑1 (MQ=255)

        aCAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAAt                                                                                                                                            >  2:5110853/1‑150 (MQ=255)

            aaaaaaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTaaa                                                                                                                                        <  1:904359/150‑1 (MQ=255)

            aaaaaaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTaaa                                                                                                                                        >  1:2524236/1‑150 (MQ=255)

             aaaaaCAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAg                                                                                                                                       <  2:1459508/150‑1 (MQ=255)

                   aGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCg                                                                                                                                 <  2:3972034/150‑1 (MQ=255)

                     tCTGACATACCGAATAGGATTAAAGGTTATATTCTCCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgat                                                                                                                               <  1:4872589/150‑1 (MQ=255)

                       tGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCgatgg                                                                                                                             >  1:1735876/1‑150 (MQ=255)

                       tGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgatgg                                                                                                                             >  1:3738020/1‑150 (MQ=255)

                          cATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCgatggatg                                                                                                                          <  1:2110702/150‑1 (MQ=255)

                           aTACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGatggatgg                                                                                                                         >  1:4283437/1‑150 (MQ=255)

                             aCCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGta                                                                                                                       <  2:3366207/150‑1 (MQ=255)

                                 aaaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATAtt                                                                                                                   >  2:820099/1‑150 (MQ=255)

                                  aaaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATAttt                                                                                                                  <  2:4056396/150‑1 (MQ=255)

                                   aaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCCGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                                 >  2:3820682/1‑150 (MQ=255)

                                   aaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                                 >  2:3051334/1‑150 (MQ=255)

                                   aaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                                 >  2:3758851/1‑150 (MQ=255)

                                   aaGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTa                                                                                                                 <  2:4774599/150‑1 (MQ=255)

                                    aGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTAc                                                                                                                >  1:1990811/1‑150 (MQ=255)

                                     ggATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACg                                                                                                               <  1:2860479/150‑1 (MQ=255)

                                     ggATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACg                                                                                                               >  1:1306150/1‑150 (MQ=255)

                                      gATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGa                                                                                                              >  2:5094003/1‑150 (MQ=255)

                                       aTTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaa                                                                                                             <  2:5030723/150‑1 (MQ=255)

                                       aTTAAAGGTTATATACACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTCAATTTGAACTCCAAATACCAGTCGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaa                                                                                                             >  2:4447942/1‑150 (MQ=255)

                                           aaGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGaaaaaa                                                                                                         >  1:160883/1‑150 (MQ=255)

                                            aGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaat                                                                                                        <  1:4516908/150‑1 (MQ=255)

                                              gTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaataa                                                                                                      <  2:1828826/150‑1 (MQ=255)

                                              gTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaataa                                                                                                      <  2:2292683/150‑1 (MQ=255)

                                               ttATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAaataat                                                                                                     <  1:496595/150‑1 (MQ=255)

                                                tataTTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAAtt                                                                                                    >  1:3141065/1‑150 (MQ=255)

                                                tataTTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAAtt                                                                                                    >  2:3007408/1‑150 (MQ=255)

                                                 atatTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtg                                                                                                   <  1:1935344/150‑1 (MQ=255)

                                                  tatTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtga                                                                                                  <  1:3661759/150‑1 (MQ=255)

                                                    ttCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATtgatg                                                                                                <  2:4334942/150‑1 (MQ=255)

                                                      cACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTgatgat                                                                                              >  2:649394/1‑150 (MQ=255)

                                                       aCCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATa                                                                                             >  1:3522078/1‑150 (MQ=255)

                                                          aGGCGATTGGTGACGTGTCTTTAGTTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAtt                                                                                          >  1:2219107/1‑150 (MQ=255)

                                                          aGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAAtt                                                                                          <  1:453542/150‑1 (MQ=255)

                                                              gATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGc                                                                                      >  1:2421883/1‑150 (MQ=255)

                                                              gATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGc                                                                                      >  2:2653412/1‑150 (MQ=255)

                                                               aTTGTTTACGTGTCTTTAGGTTATGCATTATTGTAGGATATAGTAAGCAAATTAATACGGGGTAAATTTGAACTCCAAATACCAGTAGGTTTAATTAAAGAATGCGATGTATGGTATATTTACGAAAAAATAATTGATGATAATTTTGcc                                                                                     <  2:157281/150‑1 (MQ=255)

                                                                  ggTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATa                                                                                  >  2:4110470/1‑150 (MQ=255)

                                                                  ggTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATa                                                                                  <  2:683577/150‑1 (MQ=255)

                                                                    tGACGTGTCTTTAGGTTATGCCTTATTGGATGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGt                                                                                <  2:3233222/150‑1 (MQ=255)

                                                                    tGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGt                                                                                >  2:3612265/1‑150 (MQ=255)

                                                                    tGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGt                                                                                >  2:1042535/1‑150 (MQ=255)

                                                                       cGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCAt                                                                             <  1:3780810/150‑1 (MQ=255)

                                                                        gtgtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATa                                                                            <  2:28819/150‑1 (MQ=255)

                                                                        gtgtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATa                                                                            >  1:5109002/1‑150 (MQ=255)

                                                                        gtgtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATa                                                                            >  1:305824/1‑150 (MQ=255)

                                                                          gtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGa                                                                          >  2:4239203/1‑150 (MQ=255)

                                                                          gtCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGa                                                                          >  2:596346/1‑150 (MQ=255)

                                                                          gtCTTGAGGTTATGCCTTCTTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATATGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGa                                                                          <  2:4373340/150‑1 (MQ=255)

                                                                           tCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGaa                                                                         >  1:606618/1‑150 (MQ=255)

                                                                                aGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCttt                                                                    >  2:2621606/1‑150 (MQ=255)

                                                                                 ggTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTg                                                                   <  2:170868/150‑1 (MQ=255)

                                                                                      tGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCtt                                                              <  1:4198022/150‑1 (MQ=255)

                                                                                        ccTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTg                                                            >  2:956848/1‑150 (MQ=255)

                                                                                         cTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgc                                                           >  2:3266617/1‑150 (MQ=255)

                                                                                          ttATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTgcg                                                          >  1:3818670/1‑150 (MQ=255)

                                                                                            aTTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCt                                                        >  2:1066418/1‑150 (MQ=255)

                                                                                              tGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTa                                                      <  2:1155864/150‑1 (MQ=255)

                                                                                                   gaTATAGTAAGCAAATTAACACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATc                                                 <  1:4914110/150‑1 (MQ=255)

                                                                                                      ataGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTAt                                              >  1:4034684/1‑150 (MQ=255)

                                                                                                      ataGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTAt                                              <  1:810184/150‑1 (MQ=255)

                                                                                                       taGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATg                                             <  2:3590063/150‑1 (MQ=255)

                                                                                                       taGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATg                                             <  1:3554007/150‑1 (MQ=255)

                                                                                                       taGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATg                                             <  1:1196338/150‑1 (MQ=255)

                                                                                                        aGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATgg                                            <  2:4235144/150‑1 (MQ=255)

                                                                                                           aaGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAgg                                         <  1:237576/150‑1 (MQ=255)

                                                                                                               aaaTTAAAACGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGAt                                     >  2:3423826/1‑150 (MQ=255)

                                                                                                                  ttAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGCTGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGaa                                  <  2:3913215/150‑1 (MQ=255)

                                                                                                                   tAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAAc                                 >  2:1543600/1‑150 (MQ=255)

                                                                                                                     aaaCGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGt                               >  1:834533/1‑150 (MQ=255)

                                                                                                                      aaCGGGGTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGtt                              <  2:2587276/150‑1 (MQ=255)

                                                                                                                         ggggTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTc                           >  2:1260700/1‑150 (MQ=255)

                                                                                                                         ggggTAAATTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTc                           <  2:1070993/150‑1 (MQ=255)

                                                                                                                           ggTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGt                         >  2:366414/1‑150 (MQ=255)

                                                                                                                              aaaTTTGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAAt                      >  1:3206623/1‑150 (MQ=255)

                                                                                                                                ctttGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATgg                    <  2:4942544/149‑1 (MQ=255)

                                                                                                                                 tttGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGt                   <  2:3506178/150‑1 (MQ=255)

                                                                                                                                   tGAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTc                 >  2:2161226/1‑150 (MQ=255)

                                                                                                                                   tGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTc                 <  1:2265140/150‑1 (MQ=255)

                                                                                                                                    gAACTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTcc                >  2:483141/1‑150 (MQ=255)

                                                                                                                                       cTCCAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCctct             <  1:3225336/150‑1 (MQ=255)

                                                                                                                                         ccAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgt           <  1:5134965/150‑1 (MQ=255)

                                                                                                                                          cAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgtt          >  1:1054325/1‑150 (MQ=255)

                                                                                                                                          cAAATACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgtt          <  2:1735876/150‑1 (MQ=255)

                                                                                                                                           aaaTACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgttg         <  1:2834151/150‑1 (MQ=255)

                                                                                                                                            aaTACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCtgttgt        <  1:3668235/150‑1 (MQ=255)

                                                                                                                                             aTACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTgttgtt       >  1:4801700/1‑150 (MQ=255)

                                                                                                                                              tACCAGTAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGttt      >  1:394310/1‑150 (MQ=255)

                                                                                                                                                  aggaggTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTGTTgga  >  2:4555114/1‑150 (MQ=255)

GATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTGTCTTTAGGTTATGCCTTATTGGAGGATATAGTAAGCAAATTAAAACGGGGTAAATTTGAACTCCAAATACCAGGAGGTGGAATTAAAGAATGCGATGGATGGTATATTTACGAAAAAATAATTGATGATAATTTTGCCATAGTCATAGAATCTTTGGGCTTTGCGCTTAAAATCTATGGAGGGGATGAACGTTTTCGTAATGGTTCCTCTGTTGTTTTGGA  >  CP009273/4427944‑4428239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`