Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,462,966 A→T 14.9% Y21N (TAT→AAT)  yjgH ← UPF0131 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,462,9660AT14.9% 922.0 / 138.8 316Y21N (TAT→AAT) yjgHUPF0131 family protein
Reads supporting (aligned to +/- strand):  ref base A (121/148);  new base T (30/17);  total (151/165)
Fisher's exact test for biased strand distribution p-value = 1.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

Only 100 of 321 total aligned reads displayed.

AGTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCA  >  CP009273/4462817‑4463115
                                                                                                                                                     |                                                                                                                                                     
aGTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATa                                                                                                                                                       <  2:4372873/150‑1 (MQ=255)
  tGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAAc                                                                                                                                                     <  1:4179984/150‑1 (MQ=255)
   gCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACg                                                                                                                                                    <  2:3226200/150‑1 (MQ=255)
     aTCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGAt                                                                                                                                                  >  2:2405557/1‑150 (MQ=255)
     aTCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGAt                                                                                                                                                  <  2:3576187/150‑1 (MQ=255)
        ccGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGACCGAATAGCCGCAGAATAACGATGCt                                                                                                                                               >  2:3795893/1‑150 (MQ=255)
         cGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTc                                                                                                                                              <  1:217488/150‑1 (MQ=255)
          gCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCa                                                                                                                                             >  2:284001/1‑150 (MQ=255)
           cAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAg                                                                                                                                            >  1:3548687/1‑150 (MQ=255)
            aGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGc                                                                                                                                           >  1:4339615/1‑150 (MQ=255)
             gCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCa                                                                                                                                          <  1:1219361/150‑1 (MQ=255)
                gCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCatat                                                                                                                                       >  2:63412/1‑150 (MQ=255)
                   aGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGt                                                                                                                                    <  2:89788/150‑1 (MQ=255)
                   aGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGt                                                                                                                                    >  1:3528893/1‑150 (MQ=255)
                       tCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAAt                                                                                                                                >  2:1683780/1‑150 (MQ=255)
                          cATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTc                                                                                                                             >  1:1002365/1‑150 (MQ=255)
                             gCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGc                                                                                                                          >  1:145943/1‑150 (MQ=255)
                             gCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGc                                                                                                                          <  1:4859367/150‑1 (MQ=255)
                              cAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGcc                                                                                                                         <  2:2543293/150‑1 (MQ=255)
                               aaaTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCca                                                                                                                        <  1:3060557/150‑1 (MQ=255)
                                aaTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCcag                                                                                                                       >  2:510353/1‑150 (MQ=255)
                                 aTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcagc                                                                                                                      <  2:1935434/150‑1 (MQ=255)
                                  ttATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcagca                                                                                                                     <  2:3196542/150‑1 (MQ=255)
                                       aaaTGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGaa                                                                                                                >  1:2384271/1‑150 (MQ=255)
                                           gCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACa                                                                                                            <  1:2595204/150‑1 (MQ=255)
                                           gCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACa                                                                                                            <  2:4998545/150‑1 (MQ=255)
                                            ccAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAg                                                                                                           <  1:2212217/150‑1 (MQ=255)
                                              aGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCg                                                                                                         <  1:2525569/150‑1 (MQ=255)
                                                 cTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGtt                                                                                                      >  2:3353847/1‑150 (MQ=255)
                                                     cTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttcttt                                                                                                  <  1:4950877/150‑1 (MQ=255)
                                                       tgttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttctttct                                                                                                >  2:4138540/1‑150 (MQ=255)
                                                       tgttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttctttct                                                                                                >  2:3379969/1‑150 (MQ=255)
                                                       tgttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttctttct                                                                                                <  1:4080832/150‑1 (MQ=255)
                                                        gttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTa                                                                                               <  1:1196765/150‑1 (MQ=255)
                                                        gttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTa                                                                                               <  1:3796170/150‑1 (MQ=255)
                                                          tgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAcc                                                                                             <  2:824848/150‑1 (MQ=255)
                                                          tgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAcc                                                                                             <  2:2920330/150‑1 (MQ=255)
                                                          tgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAcc                                                                                             >  1:4587837/1‑150 (MQ=255)
                                                          tgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAcc                                                                                             >  2:4680135/1‑150 (MQ=255)
                                                           gCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCa                                                                                            >  1:892423/1‑150 (MQ=255)
                                                            cTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCAt                                                                                           <  2:1042066/150‑1 (MQ=255)
                                                             tGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCAtt                                                                                          >  1:329715/1‑150 (MQ=255)
                                                              gAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCAttt                                                                                         <  1:2466685/150‑1 (MQ=255)
                                                                     gggTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATc                                                                                  >  2:1161576/1‑150 (MQ=255)
                                                                       gTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCt                                                                                >  1:618239/1‑150 (MQ=255)
                                                                        ttCTGGTGTTCCGTACTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTc                                                                               <  1:2268897/150‑1 (MQ=255)
                                                                          cTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGACAACAGCGGTTCTTTCTACCATTTGGTTATCCTcta                                                                             >  1:3126427/1‑148 (MQ=255)
                                                                          cTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCaa                                                                             <  1:3257582/150‑1 (MQ=255)
                                                                          cTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCAAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCaa                                                                             >  1:4817651/1‑150 (MQ=255)
                                                                             gtgtTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGAt                                                                          <  1:2869563/150‑1 (MQ=255)
                                                                              tgtTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGAtt                                                                         >  1:1642946/1‑150 (MQ=255)
                                                                               gtTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGAttt                                                                        <  2:3719574/150‑1 (MQ=255)
                                                                                 tCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTAc                                                                      <  2:2241568/150‑1 (MQ=255)
                                                                                 tCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTAc                                                                      >  2:1471929/1‑150 (MQ=255)
                                                                                  ccGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACg                                                                     <  1:1574798/150‑1 (MQ=255)
                                                                                  ccGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGAATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACg                                                                     >  1:2223564/1‑150 (MQ=255)
                                                                                  ccGTCCTCTCGACGTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACg                                                                     <  1:4470659/150‑1 (MQ=255)
                                                                                          tcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAAc                                                             >  2:4207937/1‑150 (MQ=255)
                                                                                          tcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAAc                                                             <  2:3962854/150‑1 (MQ=255)
                                                                                            gACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACag                                                           <  1:546928/150‑1 (MQ=255)
                                                                                             aCTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACaga                                                          <  1:4668095/150‑1 (MQ=255)
                                                                                             aCTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACaga                                                          <  1:1761069/150‑1 (MQ=255)
                                                                                                 ccTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGAt                                                      >  2:1867076/1‑150 (MQ=255)
                                                                                                       tGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTctct                                                <  2:1319581/150‑1 (MQ=255)
                                                                                                       tGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTctct                                                >  1:3462698/1‑150 (MQ=255)
                                                                                                         cccGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCttt                                              <  1:2475511/150‑1 (MQ=255)
                                                                                                          ccGGAAACAAACATCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTa                                             <  1:73830/150‑1 (MQ=255)
                                                                                                           cGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTAc                                            <  1:1797072/150‑1 (MQ=255)
                                                                                                           cGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTAc                                            >  1:2810637/1‑150 (MQ=255)
                                                                                                            ggAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGACAACAGCGGTTCTTTCTACCAGTTGGTTATCCTCAAGAGTTACGACATGAACAGAAGATTTCTCTTTAcc                                           >  2:3441840/1‑150 (MQ=255)
                                                                                                             gAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCg                                          <  2:4482669/150‑1 (MQ=255)
                                                                                                              aaacaaacAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCgg                                         <  1:3849105/150‑1 (MQ=255)
                                                                                                              aaacaaacAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCgg                                         <  1:2588489/150‑1 (MQ=255)
                                                                                                               aacaaacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCggg                                        <  2:2872903/150‑1 (MQ=255)
                                                                                                                acaaacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGa                                       <  2:20899/150‑1 (MQ=255)
                                                                                                                  aaacaGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCAGTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGc                                     >  1:2920750/1‑150 (MQ=255)
                                                                                                                        cAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCttt                               >  2:3901428/1‑150 (MQ=255)
                                                                                                                          aaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTa                             <  2:4403047/150‑1 (MQ=255)
                                                                                                                          aaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTa                             <  2:4794549/150‑1 (MQ=255)
                                                                                                                            tCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATAGAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGc                           <  2:3549926/150‑1 (MQ=255)
                                                                                                                             cGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCg                          <  1:3508860/150‑1 (MQ=255)
                                                                                                                              gCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCgg                         >  1:39698/1‑150 (MQ=255)
                                                                                                                               ccGGGACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGga                        <  2:2180070/150‑1 (MQ=255)
                                                                                                                                cGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGgac                       >  2:589975/1‑150 (MQ=255)
                                                                                                                                 ggAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGgacg                      <  1:1274315/150‑1 (MQ=255)
                                                                                                                                       aaTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCACGATTTACGACATGAACAGAAGAGTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGa                <  2:42355/150‑1 (MQ=255)
                                                                                                                                       aaTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGa                >  2:3818340/1‑150 (MQ=255)
                                                                                                                                        aTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAg               <  2:4299620/150‑1 (MQ=255)
                                                                                                                                        aTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAg               >  2:4912710/1‑150 (MQ=255)
                                                                                                                                            ccGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTaaa           <  1:2671272/150‑1 (MQ=255)
                                                                                                                                            ccGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTaaa           >  1:3444136/1‑150 (MQ=255)
                                                                                                                                             cGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAAc          <  2:4566403/150‑1 (MQ=255)
                                                                                                                                             cGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAAc          <  2:1074929/150‑1 (MQ=255)
                                                                                                                                              gCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACa         >  1:3378028/1‑150 (MQ=255)
                                                                                                                                              gCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACa         <  1:4547624/150‑1 (MQ=255)
                                                                                                                                              gCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACa         >  2:774360/1‑150 (MQ=255)
                                                                                                                                               cAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTTCGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACaa        <  1:4236132/150‑1 (MQ=255)
                                                                                                                                               cAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACaa        <  1:1986707/150‑1 (MQ=255)
                                                                                                                                                aGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACaaa       <  2:1288917/150‑1 (MQ=255)
                                                                                                                                                     aaCGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCa  <  2:22811/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCA  >  CP009273/4462817‑4463115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑