Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,514,591 G→T 6.4% V79V (GTC→GTA)  yjhH ← putative lyase/synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,514,5910GT 6.4% 1350.9 / 42.0 348V79V (GTC→GTA) yjhHputative lyase/synthase
Reads supporting (aligned to +/- strand):  ref base G (179/146);  new base T (11/12);  total (190/158)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

GTGACGCTACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGGC  >  CP009273/4514442‑4514740
                                                                                                                                                     |                                                                                                                                                     
  gACGCTACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGAc                                                                                                                                                     <  2:4943357/150‑1 (MQ=255)
       tACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCcaa                                                                                                                                                >  1:4823617/1‑150 (MQ=255)
         cGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCTACGCcaatc                                                                                                                                              >  2:3053381/1‑150 (MQ=255)
         cGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCTACGCcaatc                                                                                                                                              >  2:3360149/1‑150 (MQ=255)
         cGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCcaatc                                                                                                                                              >  2:4064619/1‑150 (MQ=255)
          gggCGATCTGCTGGTAATTGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGATGGGGAACCGACGCcaatca                                                                                                                                             <  2:4686035/150‑1 (MQ=255)
          gggCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCcaatca                                                                                                                                             >  1:1205409/1‑150 (MQ=255)
           ggCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCTACGCcaatcaa                                                                                                                                            >  1:3313440/1‑150 (MQ=255)
                  tgctgGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGc                                                                                                                                     >  2:4044616/1‑150 (MQ=255)
                       gTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCg                                                                                                                                >  1:2365762/1‑150 (MQ=255)
                           tAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCg                                                                                                                            <  1:1428283/150‑1 (MQ=255)
                            aGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGt                                                                                                                           >  1:3336336/1‑150 (MQ=255)
                            aGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGt                                                                                                                           >  2:4367705/1‑150 (MQ=255)
                              tcgtcAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCg                                                                                                                         <  2:2689348/150‑1 (MQ=255)
                               cgtcAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGa                                                                                                                        >  1:3834421/1‑150 (MQ=255)
                                gtcAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGAc                                                                                                                       <  2:720121/150‑1 (MQ=255)
                                   aaGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAAt                                                                                                                    >  1:3020820/1‑150 (MQ=255)
                                    aGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCTACGCCAATCAATACCGGCACTCGCCCGTCGACAATg                                                                                                                   <  2:1669829/150‑1 (MQ=255)
                                       tttCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGtt                                                                                                                >  2:3643300/1‑150 (MQ=255)
                                        ttCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTa                                                                                                               <  2:4134820/150‑1 (MQ=255)
                                          cGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCTACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACa                                                                                                             <  1:1765075/150‑1 (MQ=255)
                                          cGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACa                                                                                                             <  1:4658417/150‑1 (MQ=255)
                                          cGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACa                                                                                                             <  1:1004017/150‑1 (MQ=255)
                                            tggtgCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGc                                                                                                           >  1:1839745/1‑150 (MQ=255)
                                                 cGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGcttctt                                                                                                      >  1:2713104/1‑150 (MQ=255)
                                                  gACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCttcttc                                                                                                     <  2:2474451/150‑1 (MQ=255)
                                                  gACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCttcttc                                                                                                     >  1:2955380/1‑150 (MQ=255)
                                                   aCTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCg                                                                                                    <  1:3747486/150‑1 (MQ=255)
                                                   aCTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCg                                                                                                    >  1:4461063/1‑150 (MQ=255)
                                                     tttCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCTACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGc                                                                                                  >  2:2795069/1‑150 (MQ=255)
                                                      ttCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCg                                                                                                 >  2:6609/1‑150 (MQ=255)
                                                                agGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATgc                                                                                       <  1:5098592/150‑1 (MQ=255)
                                                                agGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATgc                                                                                       <  2:4489487/150‑1 (MQ=255)
                                                                   ggTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCt                                                                                    <  2:2853054/150‑1 (MQ=255)
                                                                   ggTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGATTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCt                                                                                    <  2:1252212/150‑1 (MQ=255)
                                                                      tGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTggg                                                                                 >  2:4611174/1‑150 (MQ=255)
                                                                       gATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGc                                                                                <  1:4016445/150‑1 (MQ=255)
                                                                         tGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTg                                                                              <  2:1334346/150‑1 (MQ=255)
                                                                            cgacgaTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTAt                                                                           <  1:4178036/150‑1 (MQ=255)
                                                                            cgacgaTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTAt                                                                           <  1:78910/150‑1 (MQ=255)
                                                                             gacgaTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTAtt                                                                          >  1:495968/1‑150 (MQ=255)
                                                                             gacgaTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTAtt                                                                          >  1:1698310/1‑150 (MQ=255)
                                                                              acgaTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCTACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTc                                                                         <  1:1785518/150‑1 (MQ=255)
                                                                              acgaTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTc                                                                         >  1:1292414/1‑150 (MQ=255)
                                                                               cgaTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCa                                                                        >  2:1086557/1‑150 (MQ=255)
                                                                                gaTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCAt                                                                       >  2:515887/1‑150 (MQ=255)
                                                                                 aTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGTAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGAGCTTGGCTGTATTCAtt                                                                      >  2:4389159/1‑150 (MQ=255)
                                                                                 aTACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCAtt                                                                      >  2:1911489/1‑150 (MQ=255)
                                                                                  tACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCAttt                                                                     <  1:2300055/150‑1 (MQ=255)
                                                                                   aCCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTg                                                                    >  2:4894022/1‑150 (MQ=255)
                                                                                   aCCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTg                                                                    <  2:4424652/150‑1 (MQ=255)
                                                                                   aCCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTg                                                                    >  2:1500054/1‑150 (MQ=255)
                                                                                    ccATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCTACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTgg                                                                   >  1:2151979/1‑150 (MQ=255)
                                                                                    ccATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTgg                                                                   >  1:3120602/1‑150 (MQ=255)
                                                                                      aTCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCt                                                                 >  1:604772/1‑150 (MQ=255)
                                                                                       tCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTa                                                                >  2:2565398/1‑150 (MQ=255)
                                                                                         aGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGACCCGACGCCAATCAATACCGGCACTCGCCCGTCGACACGGGTTACAGCTTCTTCGGCGAGAGCCATGCGCTGGGCTGTAGTCATTTGGCTaaa                                                              >  2:2708582/1‑150 (MQ=255)
                                                                                         aGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTaaa                                                              >  1:5063649/1‑150 (MQ=255)
                                                                                           cgcCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAAtt                                                            <  2:1767046/150‑1 (MQ=255)
                                                                                            gcCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTc                                                           <  2:3418088/150‑1 (MQ=255)
                                                                                             ccGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTca                                                          >  2:3870639/1‑150 (MQ=255)
                                                                                                 aGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTcaccac                                                      <  1:2029414/150‑1 (MQ=255)
                                                                                                 aGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTcaccac                                                      >  1:2527178/1‑150 (MQ=255)
                                                                                                    cTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCgg                                                   <  2:2386395/150‑1 (MQ=255)
                                                                                                    cTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCgg                                                   <  2:4220424/150‑1 (MQ=255)
                                                                                                     ttGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCTACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGt                                                  <  1:2657030/150‑1 (MQ=255)
                                                                                                     ttGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGt                                                  <  2:528569/150‑1 (MQ=255)
                                                                                                         gcATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTAccc                                              >  1:3562529/1‑150 (MQ=255)
                                                                                                          cATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCa                                             <  2:4819271/150‑1 (MQ=255)
                                                                                                           aTGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAg                                            >  1:616027/1‑150 (MQ=255)
                                                                                                           aTGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAg                                            <  1:214378/150‑1 (MQ=255)
                                                                                                           aTGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAg                                            >  2:3229498/1‑150 (MQ=255)
                                                                                                                  gcCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAAc                                     >  2:1608350/1‑150 (MQ=255)
                                                                                                                    cAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAg                                   >  2:455912/1‑150 (MQ=255)
                                                                                                                     aGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGc                                  >  1:1110377/1‑150 (MQ=255)
                                                                                                                     aGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGc                                  >  1:3484689/1‑150 (MQ=255)
                                                                                                                     aGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGc                                  <  2:2365762/150‑1 (MQ=255)
                                                                                                                       tttGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGccc                                >  1:4609780/1‑150 (MQ=255)
                                                                                                                       tttGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGccc                                >  1:3884018/1‑150 (MQ=255)
                                                                                                                         tGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGt                              <  2:3834421/150‑1 (MQ=255)
                                                                                                                          gACCGCTTCGTCAGTGGAAGGGGAACCTACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTc                             >  1:1630925/1‑150 (MQ=255)
                                                                                                                          gACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTc                             <  1:2800655/150‑1 (MQ=255)
                                                                                                                           aCCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCg                            <  1:3442784/150‑1 (MQ=255)
                                                                                                                            ccGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGa                           >  2:1889617/1‑150 (MQ=255)
                                                                                                                              gCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGAcc                         <  2:3722909/150‑1 (MQ=255)
                                                                                                                                 tCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCt                      <  2:552109/150‑1 (MQ=255)
                                                                                                                                  cGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCtt                     <  2:4528660/150‑1 (MQ=255)
                                                                                                                                  cGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCtt                     >  2:4040792/1‑150 (MQ=255)
                                                                                                                                   gTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCttt                    >  1:3271772/1‑150 (MQ=255)
                                                                                                                                   gTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCttt                    >  1:3129015/1‑150 (MQ=255)
                                                                                                                                     cAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTttat                  <  2:272184/150‑1 (MQ=255)
                                                                                                                                           aaGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCa            <  1:2066874/150‑1 (MQ=255)
                                                                                                                                             ggggAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAgg          <  2:2032186/150‑1 (MQ=255)
                                                                                                                                              gggAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGa         <  2:2946941/150‑1 (MQ=255)
                                                                                                                                               ggAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGaa        <  2:2150438/150‑1 (MQ=255)
                                                                                                                                                gAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAg       >  2:2991714/1‑150 (MQ=255)
                                                                                                                                                gAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAg       <  2:3275219/150‑1 (MQ=255)
                                                                                                                                                   ccGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCg    >  1:926987/1‑150 (MQ=255)
                                                                                                                                                   ccGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCg    <  1:4376087/150‑1 (MQ=255)
                                                                                                                                                    cGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCgg   >  1:4336809/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGACGCTACGGGCGATCTGCTGGTAATAGTCGTCAAGATTTCGTGGTGCGACTTTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGGC  >  CP009273/4514442‑4514740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑