Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,604,966 G→T 7.9% A132E (GCG→GAG)  yjjW ← putative pyruvate formate lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,604,9660GT 7.9% 1175.3 / 50.6 315A132E (GCG→GAG) yjjWputative pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base G (138/152);  new base T (15/10);  total (153/162)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 323 total aligned reads displayed.

ATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGG  >  CP009273/4604817‑4605115
                                                                                                                                                     |                                                                                                                                                     
aTTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAgcg                                                                                                                                                       <  2:2956147/150‑1 (MQ=255)
  tCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCTcc                                                                                                                                                     >  1:4587902/1‑150 (MQ=255)
  tCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGcc                                                                                                                                                     <  2:5040084/150‑1 (MQ=255)
      tCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGccacca                                                                                                                                                 <  2:4077480/150‑1 (MQ=255)
          ccACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCTCCACCACaaa                                                                                                                                             >  2:3252321/1‑150 (MQ=255)
           cACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAAc                                                                                                                                            <  2:4341840/150‑1 (MQ=255)
           cACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAAc                                                                                                                                            <  1:1309248/150‑1 (MQ=255)
           cACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTGGATAGCAGTAAACCGCGCCACCACAAAc                                                                                                                                            >  2:4299331/1‑150 (MQ=255)
            aCGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACg                                                                                                                                           >  1:89701/1‑150 (MQ=255)
            aCGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACg                                                                                                                                           <  1:4934894/150‑1 (MQ=255)
              gCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGc                                                                                                                                         <  1:4972861/150‑1 (MQ=255)
               cTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCa                                                                                                                                        >  2:4893972/1‑150 (MQ=255)
                tttGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAg                                                                                                                                       <  2:2320540/150‑1 (MQ=255)
                  tGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCt                                                                                                                                     >  1:436514/1‑150 (MQ=255)
                       tCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTc                                                                                                                                <  1:350067/150‑1 (MQ=255)
                         gAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCTCCACCACAAACGGCAGCTGGGTCGt                                                                                                                              >  2:2412684/1‑150 (MQ=255)
                         gAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGt                                                                                                                              >  1:4633518/1‑150 (MQ=255)
                          aGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTg                                                                                                                             <  1:1860925/150‑1 (MQ=255)
                             aTTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCt                                                                                                                          <  2:2938812/150‑1 (MQ=255)
                             aTTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCt                                                                                                                          >  1:1824040/1‑150 (MQ=255)
                             aTTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCt                                                                                                                          >  1:3729286/1‑150 (MQ=255)
                             aTTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCt                                                                                                                          >  1:4379142/1‑150 (MQ=255)
                               tGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTc                                                                                                                        <  2:2117520/150‑1 (MQ=255)
                                 cgcCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCAc                                                                                                                      >  2:1862825/1‑150 (MQ=255)
                                  gcCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCAcc                                                                                                                     <  2:2014848/150‑1 (MQ=255)
                                  gcCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCAcc                                                                                                                     <  2:1662258/150‑1 (MQ=255)
                                   ccGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCg                                                                                                                    <  1:3659512/150‑1 (MQ=255)
                                    cGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGc                                                                                                                   >  1:1634519/1‑150 (MQ=255)
                                      tCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCa                                                                                                                 >  2:1197920/1‑150 (MQ=255)
                                       cGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCAc                                                                                                                >  2:3088187/1‑150 (MQ=255)
                                       cGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCAc                                                                                                                <  1:4323270/150‑1 (MQ=255)
                                        gCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACt                                                                                                               >  2:5006064/1‑150 (MQ=255)
                                          acacCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCa                                                                                                             >  2:1313817/1‑150 (MQ=255)
                                            acCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCAcc                                                                                                           >  2:1264990/1‑150 (MQ=255)
                                               gggAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGtt                                                                                                        >  2:3319506/1‑150 (MQ=255)
                                                  aGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCTCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATc                                                                                                     <  2:277433/150‑1 (MQ=255)
                                                      aTTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCtt                                                                                                 >  1:2726187/1‑150 (MQ=255)
                                                          ttCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCTCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGAt                                                                                             >  1:2089379/1‑150 (MQ=255)
                                                          ttCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGAt                                                                                             <  2:3828887/150‑1 (MQ=255)
                                                          ttCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGAt                                                                                             <  2:1368032/150‑1 (MQ=255)
                                                          ttCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGAt                                                                                             <  2:3828685/150‑1 (MQ=255)
                                                           tCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATa                                                                                            <  2:3709093/150‑1 (MQ=255)
                                                            cccAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCTCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATaa                                                                                           >  1:1250556/1‑150 (MQ=255)
                                                             ccAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATaaa                                                                                          <  2:3532231/150‑1 (MQ=255)
                                                               aGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACa                                                                                        <  2:4689966/150‑1 (MQ=255)
                                                               aGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACa                                                                                        >  1:1363965/1‑150 (MQ=255)
                                                                gCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAg                                                                                       >  1:2349187/1‑150 (MQ=255)
                                                                  cGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCa                                                                                     >  1:4515530/1‑150 (MQ=255)
                                                                   ggTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCAc                                                                                    >  1:1752343/1‑150 (MQ=255)
                                                                   ggTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGGTATCCCTTCGATAAACAGCAc                                                                                    >  2:630619/1‑150 (MQ=255)
                                                                    gTTTCTCTCCACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCTTTTTTGATAGAAGTAAACAGCGCCACCACATACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACt                                                                                   <  1:4332959/150‑1 (MQ=255)
                                                                    gTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACt                                                                                   <  2:4727600/150‑1 (MQ=255)
                                                                     tttCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTg                                                                                  >  1:4723021/1‑150 (MQ=255)
                                                                         gCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCttt                                                                              >  2:2354045/1‑150 (MQ=255)
                                                                           tCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGc                                                                            <  1:4703207/150‑1 (MQ=255)
                                                                             aaCATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCgg                                                                          >  2:3201316/1‑150 (MQ=255)
                                                                              aCATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGa                                                                         <  1:2965135/150‑1 (MQ=255)
                                                                              aCATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGa                                                                         <  1:1713519/150‑1 (MQ=255)
                                                                                 tGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACAt                                                                      <  2:4274565/150‑1 (MQ=255)
                                                                                 tGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACAt                                                                      >  2:4568804/1‑150 (MQ=255)
                                                                                 tGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACAt                                                                      >  1:2535126/1‑150 (MQ=255)
                                                                                 tGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACAt                                                                      >  1:222733/1‑150 (MQ=255)
                                                                                    cGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGc                                                                   >  2:1063109/1‑150 (MQ=255)
                                                                                    cGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGc                                                                   >  2:758634/1‑150 (MQ=255)
                                                                                       tACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTaa                                                                <  1:2891823/150‑1 (MQ=255)
                                                                                         cTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGc                                                              >  2:4449208/1‑150 (MQ=255)
                                                                                         cTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGc                                                              >  1:2582517/1‑150 (MQ=255)
                                                                                         cTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGc                                                              <  1:2256421/150‑1 (MQ=255)
                                                                                          tGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCTCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCa                                                             <  1:2431535/150‑1 (MQ=255)
                                                                                          tGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCa                                                             >  2:3742882/1‑150 (MQ=255)
                                                                                            tCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACt                                                           <  2:678911/150‑1 (MQ=255)
                                                                                             ccaccaGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACtt                                                          >  1:1197310/1‑150 (MQ=255)
                                                                                              caccaGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTc                                                         <  1:4461910/150‑1 (MQ=255)
                                                                                                    aCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCAc                                                   >  1:3871482/1‑150 (MQ=255)
                                                                                                    aCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCAc                                                   >  2:2108312/1‑150 (MQ=255)
                                                                                                    aCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCAc                                                   <  2:4051688/150‑1 (MQ=255)
                                                                                                      aGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGc                                                 >  2:3682127/1‑150 (MQ=255)
                                                                                                        gTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTc                                               >  2:1575329/1‑150 (MQ=255)
                                                                                                          gagaTGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCAt                                             >  2:1963381/1‑150 (MQ=255)
                                                                                                          gagaTGGCGCAGTTGCGGAACGTTTTTGATAGCACTAAACAGCGCCACCACAAACGCCACCAGGCACGAGGATTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTATGCGGACATGGCTAAGCACTTCGTCAACGCTCAt                                             >  2:3884313/1‑150 (MQ=255)
                                                                                                              tGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGAt                                         <  2:115014/150‑1 (MQ=255)
                                                                                                               ggCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGAtt                                        <  2:253579/150‑1 (MQ=255)
                                                                                                                  gcAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTgcg                                     <  2:2291054/150‑1 (MQ=255)
                                                                                                                   cAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTgcgc                                    >  1:2793280/1‑150 (MQ=255)
                                                                                                                    aGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGcc                                   >  1:3773787/1‑150 (MQ=255)
                                                                                                                      ttGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCAt                                 <  2:4498622/150‑1 (MQ=255)
                                                                                                                       tGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATg                                >  2:2171115/1‑150 (MQ=255)
                                                                                                                                  tttGATAGCAGTAAACAGCGCCACCACAAACGTCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATTAACAGCACTGCTTTGCGGACATGGCTAAGAACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATg                     <  1:3192637/150‑1 (MQ=255)
                                                                                                                                  tttGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATg                     <  1:3192545/150‑1 (MQ=255)
                                                                                                                                    tGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGtt                   >  1:2041345/1‑150 (MQ=255)
                                                                                                                                     gATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTg                  >  1:100130/1‑150 (MQ=255)
                                                                                                                                     gATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGATAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTg                  <  1:4862159/150‑1 (MQ=255)
                                                                                                                                      aTAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGc                 >  1:4289257/1‑150 (MQ=255)
                                                                                                                                        aGCAGTAAACAGCGCCACCACAAACTGCAGCTGGGACGTGGCTTCACCGCCACTCACCGTTATCACTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCgg               <  2:3561338/150‑1 (MQ=255)
                                                                                                                                           aGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGaca            >  1:2385122/1‑150 (MQ=255)
                                                                                                                                            gTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGacac           <  1:658822/150‑1 (MQ=255)
                                                                                                                                             tAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACAcc          <  1:4872677/150‑1 (MQ=255)
                                                                                                                                              aaaCAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACct         <  2:895903/150‑1 (MQ=255)
                                                                                                                                                 cAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCtt      <  1:4125542/150‑1 (MQ=255)
                                                                                                                                                   gcgcCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTca    <  2:4515896/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGG  >  CP009273/4604817‑4605115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑