Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,609,367 A→T 7.0% *441L (TAA→TTA)  deoA → thymidine phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,609,3670AT 7.0% 1254.4 / 62.3 375*441L (TAA→TTA) deoAthymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (142/206);  new base T (11/16);  total (153/222)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 380 total aligned reads displayed.

CCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTC  >  CP009273/4609218‑4609516
                                                                                                                                                     |                                                                                                                                                     
   ggTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACg                                                                                                                                                    <  2:3646791/150‑1 (MQ=255)
   ggTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACg                                                                                                                                                    <  2:3798603/150‑1 (MQ=255)
      aGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGta                                                                                                                                                 <  1:4510178/150‑1 (MQ=255)
        aCGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGtata                                                                                                                                               <  1:629350/150‑1 (MQ=255)
        aCGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGtata                                                                                                                                               <  2:1550800/150‑1 (MQ=255)
         cGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATAc                                                                                                                                              <  1:2016661/150‑1 (MQ=255)
         cGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATAc                                                                                                                                              <  1:361780/150‑1 (MQ=255)
          ggTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATAct                                                                                                                                             <  2:1432732/150‑1 (MQ=255)
            tCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATActga                                                                                                                                           >  2:5144254/1‑150 (MQ=255)
             cAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATActgat                                                                                                                                          <  1:913132/150‑1 (MQ=255)
              aGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATActgatc                                                                                                                                         >  1:4493066/1‑150 (MQ=255)
              aGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATActgatc                                                                                                                                         >  2:4940204/1‑150 (MQ=255)
              aGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATActgatc                                                                                                                                         <  1:3716309/150‑1 (MQ=255)
                cGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATActgatctg                                                                                                                                       <  2:1987905/150‑1 (MQ=255)
                cGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATActgatctg                                                                                                                                       <  1:2948753/150‑1 (MQ=255)
                 gTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATTACGGTATActgatctga                                                                                                                                      >  2:4226180/1‑150 (MQ=255)
                  tCCGCTGGAGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCTAATGCGGTGAATGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATActtatctgat                                                                                                                                     <  2:542674/150‑1 (MQ=255)
                    cGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCa                                                                                                                                   <  1:1928298/150‑1 (MQ=255)
                     gCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCAt                                                                                                                                  >  1:1587545/1‑150 (MQ=255)
                      cTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCAtt                                                                                                                                 <  2:1006753/150‑1 (MQ=255)
                           ggTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAAt                                                                                                                            <  1:2856404/150‑1 (MQ=255)
                             ttATCCACGCGAAAGACGAAGACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATTACGGTATACTGATCTGATCATTTAAAttt                                                                                                                          <  1:4601210/150‑1 (MQ=255)
                              tATCCACGCGAAAGACGAAAACAACTGGCAGGACGCGGCGAAAGCGGTGAAAGCGGCAAGTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTCTCAGCGAATACCGGTATACTGATCTGATCATGTAAAGTTg                                                                                                                         >  2:4501885/1‑150 (MQ=255)
                               aTCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATActgatctgat                                                                                                                                     <  2:1370395/137‑1 (MQ=255)
                                       gAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGa                                                                                                                <  2:3131805/150‑1 (MQ=255)
                                         aaGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGt                                                                                                              <  1:140086/150‑1 (MQ=255)
                                          aGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTa                                                                                                             <  2:3601374/150‑1 (MQ=255)
                                          aGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCAATGAGTa                                                                                                             <  2:2149940/150‑1 (MQ=255)
                                            aCGAAAACAACTGGCAGGAAGCGGCGAATGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCCACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACg                                                                                                           <  2:2523662/150‑1 (MQ=255)
                                            aCGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACg                                                                                                           <  2:2677656/150‑1 (MQ=255)
                                              gAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGga                                                                                                         <  1:343576/150‑1 (MQ=255)
                                               aaaaCAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGgag                                                                                                        >  2:1404790/1‑150 (MQ=255)
                                                   caacTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAAGGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACa                                                                                                    <  1:467136/150‑1 (MQ=255)
                                                    aacTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACat                                                                                                   <  2:2367485/150‑1 (MQ=255)
                                                    aacTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACat                                                                                                   <  2:2260238/150‑1 (MQ=255)
                                                      cTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATTACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACatat                                                                                                 <  1:21569/150‑1 (MQ=255)
                                                        ggCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGa                                                                                               <  1:3173972/150‑1 (MQ=255)
                                                        ggCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGa                                                                                               <  2:840851/150‑1 (MQ=255)
                                                           aGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAAc                                                                                            >  1:2374253/1‑150 (MQ=255)
                                                              aaGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTg                                                                                         >  2:38940/1‑150 (MQ=255)
                                                              aaGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTg                                                                                         >  1:663863/1‑150 (MQ=255)
                                                               aGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGc                                                                                        >  2:3290653/1‑150 (MQ=255)
                                                                gcggcgAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCa                                                                                       <  1:2506960/150‑1 (MQ=255)
                                                                    cgAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATtta                                                                                   <  1:4539184/150‑1 (MQ=255)
                                                                      aaaGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATttatt                                                                                 <  1:3017162/150‑1 (MQ=255)
                                                                      aaaGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATttatt                                                                                 >  2:4301161/1‑150 (MQ=255)
                                                                        aGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTtattat                                                                               <  2:2081300/150‑1 (MQ=255)
                                                                         gCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATg                                                                              <  2:430366/150‑1 (MQ=255)
                                                                          cGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATgg                                                                             <  2:3004635/150‑1 (MQ=255)
                                                                          cGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATgg                                                                             <  2:916331/150‑1 (MQ=255)
                                                                          cGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATgg                                                                             >  1:3796433/1‑150 (MQ=255)
                                                                           ggTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGt                                                                            >  2:3518242/1‑150 (MQ=255)
                                                                           ggTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGt                                                                            >  1:5112294/1‑150 (MQ=255)
                                                                           ggTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGt                                                                            >  1:1622413/1‑150 (MQ=255)
                                                                           ggTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGt                                                                            >  1:4062359/1‑150 (MQ=255)
                                                                            gTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTg                                                                           >  1:1094177/1‑150 (MQ=255)
                                                                            gTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTg                                                                           <  2:2846436/150‑1 (MQ=255)
                                                                            gTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTg                                                                           >  1:3835831/1‑150 (MQ=255)
                                                                             tGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATTACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGc                                                                          >  2:466085/1‑150 (MQ=255)
                                                                              gAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCt                                                                         >  1:40017/1‑150 (MQ=255)
                                                                               aaaGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTg                                                                        >  1:364024/1‑150 (MQ=255)
                                                                                 aGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGa                                                                      >  2:4702582/1‑150 (MQ=255)
                                                                                  gCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGAc                                                                     <  2:4140754/150‑1 (MQ=255)
                                                                                   cGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACt                                                                    <  2:4858929/150‑1 (MQ=255)
                                                                                    ggCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTc                                                                   >  2:5049836/1‑150 (MQ=255)
                                                                                     gCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCa                                                                  <  1:4214979/150‑1 (MQ=255)
                                                                                        aTTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTc                                                               <  2:4813146/150‑1 (MQ=255)
                                                                                        aTTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTc                                                               <  2:1208414/150‑1 (MQ=255)
                                                                                         ttAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCg                                                              <  1:219542/150‑1 (MQ=255)
                                                                                             aCTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCAt                                                          <  1:4900502/150‑1 (MQ=255)
                                                                                             aCTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCAt                                                          >  1:2713459/1‑150 (MQ=255)
                                                                                               ttGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCg                                                        <  1:860499/150‑1 (MQ=255)
                                                                                               ttGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCg                                                        <  2:3239045/150‑1 (MQ=255)
                                                                                                   cGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGgcgc                                                    <  1:1496323/150‑1 (MQ=255)
                                                                                                     aTAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTa                                                  >  2:3972756/1‑150 (MQ=255)
                                                                                                      tAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTAc                                                 <  1:4301951/150‑1 (MQ=255)
                                                                                                      tAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTAc                                                 <  2:1798846/150‑1 (MQ=255)
                                                                                                       aaaGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACa                                                >  1:3348037/1‑150 (MQ=255)
                                                                                                        aaGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACag                                               >  1:1155636/1‑150 (MQ=255)
                                                                                                         aGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACaga                                              >  1:2122629/1‑150 (MQ=255)
                                                                                                           cACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATCCTGATCTGATCATTTCAATTTGAAGCACTGAGTACGGAGAACATATGCAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACagaag                                            <  2:4040594/150‑1 (MQ=255)
                                                                                                               ggAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGc                                        >  1:2959731/1‑150 (MQ=255)
                                                                                                                    gCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAAc                                   <  2:3084518/150‑1 (MQ=255)
                                                                                                                    gCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAAc                                   <  2:3064180/150‑1 (MQ=255)
                                                                                                                         ccAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCttt                              <  1:2278516/150‑1 (MQ=255)
                                                                                                                           aaCTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTgg                            >  2:321029/1‑150 (MQ=255)
                                                                                                                             cTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTg                          >  2:1713709/1‑150 (MQ=255)
                                                                                                                             cTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGGACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTg                          <  1:2822966/150‑1 (MQ=255)
                                                                                                                              tGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGa                         <  2:4619679/150‑1 (MQ=255)
                                                                                                                                 cTATCGCCGTATCAGCGAATTACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGt                      <  1:1306305/150‑1 (MQ=255)
                                                                                                                                   aTCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCg                    <  2:1144042/150‑1 (MQ=255)
                                                                                                                                   aTCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCg                    <  1:1656495/150‑1 (MQ=255)
                                                                                                                                     cGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCggg                  <  1:4588244/150‑1 (MQ=255)
                                                                                                                                       ccGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGc                >  2:3354883/1‑150 (MQ=255)
                                                                                                                                         gTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTg              >  1:1120303/1‑150 (MQ=255)
                                                                                                                                          tATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGGACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGa             <  2:71674/150‑1 (MQ=255)
                                                                                                                                            tCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGaca           <  1:4331804/150‑1 (MQ=255)
                                                                                                                                            tCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGaca           <  1:1774646/150‑1 (MQ=255)
                                                                                                                                                cGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCGTTGGTGACGTCGGGGCTGACACCCt       >  2:3469818/1‑150 (MQ=255)
                                                                                                                                                  aaTAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTgg     >  2:4733720/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACATATGAAACGTGCATTTATTATGGTGCTGGACTCATTCGGCATCGGCGCTACAGAAGATGCAGAACGCTTTGGTGACGTCGGGGCTGACACCCTGGGTC  >  CP009273/4609218‑4609516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑