BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,629,324	G→T	9.7%	D444Y (GAT→TAT)	creD →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,629,324	0	G	T	9.7%	871.8 / 33.3	279	D444Y (GAT→TAT)	creD	inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (114/131); new base T (6/28); total (120/159)
Fisher's exact test for biased strand distribution p-value = 1.43e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

ACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTA  >  CP009273/4629176‑4629473

aCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGa                                                                                                                                                      >  1:1132034/1‑150 (MQ=255)

aCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGa                                                                                                                                                      >  1:3718221/1‑150 (MQ=255)

    cTCAACTCTGCCGATAGCTCGCTGTTGTTGGGAACCTGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCAGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAg                                                                                                                                                  <  1:688933/150‑1 (MQ=255)

    cTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACTATGAg                                                                                                                                                  <  1:1274798/150‑1 (MQ=255)

      cAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGtt                                                                                                                                                <  2:380581/150‑1 (MQ=255)

      cAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGtt                                                                                                                                                <  2:4141991/150‑1 (MQ=255)

      cAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGtt                                                                                                                                                >  2:3197082/1‑150 (MQ=255)

       aaCTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACTATGAGTTa                                                                                                                                               >  2:83380/1‑150 (MQ=255)

       aaCTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTa                                                                                                                                               >  2:1576315/1‑150 (MQ=255)

        aCTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTAc                                                                                                                                              <  2:4856726/150‑1 (MQ=255)

        aCTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTAc                                                                                                                                              <  2:4327352/150‑1 (MQ=255)

         ctctGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACg                                                                                                                                             <  1:216614/150‑1 (MQ=255)

          tcggCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGt                                                                                                                                            <  1:1460728/147‑1 (MQ=255)

             gCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATc                                                                                                                                         <  2:6442/150‑1 (MQ=255)

             gCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATc                                                                                                                                         <  2:1166028/150‑1 (MQ=255)

              ccGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCt                                                                                                                                        <  2:4314847/150‑1 (MQ=255)

                gATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTgg                                                                                                                                      <  1:4312892/150‑1 (MQ=255)

                  tAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACTATGAGTTACGTATCTGGaa                                                                                                                                    <  1:2115899/150‑1 (MQ=255)

                  tAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGaa                                                                                                                                    >  2:4773922/1‑150 (MQ=255)

                    gcgcgcTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAAt                                                                                                                                  <  2:1809671/150‑1 (MQ=255)

                      gcgcTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATaa                                                                                                                                >  2:4895858/1‑150 (MQ=255)

                       cgcTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAg                                                                                                                               <  1:1812426/150‑1 (MQ=255)

                          tgttgtTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGtt                                                                                                                            <  1:2977596/150‑1 (MQ=255)

                            ttgttgGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGa                                                                                                                          <  2:917907/150‑1 (MQ=255)

                              gttgGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGaaa                                                                                                                        <  2:1965708/150‑1 (MQ=255)

                               ttgGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATACGGTTGaaaa                                                                                                                       >  1:4853172/1‑150 (MQ=255)

                                  ggAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATa                                                                                                                    >  1:2720326/1‑150 (MQ=255)

                                   gAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATaa                                                                                                                   <  2:3464466/150‑1 (MQ=255)

                                    aaCCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATaaa                                                                                                                  <  1:3243042/150‑1 (MQ=255)

                                    aaCCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATaaa                                                                                                                  >  2:4382930/1‑150 (MQ=255)

                                          tgtgCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGgc                                                                                                            <  2:2146250/150‑1 (MQ=255)

                                           gtgCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACTATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGgcg                                                                                                           >  1:3210242/1‑150 (MQ=255)

                                           gtgCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAATCGgcg                                                                                                           >  2:5118571/1‑150 (MQ=255)

                                              cTGGGGGGGGCGCTGGCCGGCTTGTTGTTTGTGACCCTTAATTTCGACTGGTTTGCGTTTTCACTGCCTAAAATGAAAGCCTGTAAATAATTTAAAACGGACGATGAGTTACGTATCTGGAAATATGGTTGAAAAATAAAAACGGCGCTa                                                                                                        <  2:4521514/150‑1 (MQ=255)

                                               tggtggtggCGGGGGCCGGATTTTTGTTTGTGACCCGTAATATCGATTGGTTTGCGTTTTCACTGCCGAAATTGAAAGCCAGTAAATAATTTACAAGGGACGTTTAGTTACGTATCTGGAAATAAGGTTGAAAATTAAAAACGGCGCTaa                                                                                                       <  2:3680357/150‑1 (MQ=255)

                                               aggtggtggCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTaa                                                                                                       >  2:1713959/2‑150 (MQ=255)

                                                tttggtggCGCTGGCGGGCATGTTTTTTGTGACCCTTAATATCTACTGTTATGCTTTTTAACTGCCTAAAATGAAAGCCTTTAAATATTTTACAAGTGAGTATGATTTACTTTTCTGTAAATATGTTTTAAAAATAAAAACGGCGCTaaa                                                                                                      <  2:1103627/148‑1 (MQ=255)

                                                    gtggCGCTGGCCGGCTTGATGTTTGTGACCCTTAATATCGACTGGTATGCGTTTTAACTGCCTAAAATGAAAGCCATTAAATAATTTACAACGGACTATTAGTTACGTATCTGTAAATAAGGTTTAAAAATAAAAACGGCGCTAAAAAgc                                                                                                  <  2:1412025/150‑1 (MQ=255)

                                                     tggCGCTGGCCGGCTTGATGTTTGTGACCCTTAATATCGACTGGTATGCGTTTTCACTGCCGAAATTGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATATGGTTTAAAAATAAAAACGGCGCTAAAAAgcg                                                                                                 <  2:2001332/150‑1 (MQ=255)

                                                           tGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGttt                                                                                           >  2:938464/1‑150 (MQ=255)

                                                           tGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGttt                                                                                           >  1:4406671/1‑150 (MQ=255)

                                                                ggCAGGAGGTTTGTGACCGGTATTATCGACTGGTATGCGTTTTCACTGCCGAAAATGAACGCCAGTAAAGACGTTACAACGGACGATGTGTTAGGTATCTGGAAATAAGGTTGAAAAATAAACACGGCGCTAAAAAGCGCCGtttttttt                                                                                      <  2:213746/150‑1 (MQ=255)

                                                                 gCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTg                                                                                     >  1:5020023/1‑150 (MQ=255)

                                                                   atgatgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGAc                                                                                   >  2:3337617/1‑150 (MQ=255)

                                                                       tgTTTGTGACCCGTAATTTCGCGTGGTTTGGGTTTTCACTGCCTAAAATGAATGCCAGTATTGAAGTTACACCGGACGTTGTTTTACGTTTCTGGAAATATGGTTTAAAATTAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACggtg                                                                               <  2:2175936/150‑1 (MQ=255)

                                                                       tgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACggtg                                                                               >  1:444143/1‑150 (MQ=255)

                                                                       tgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACggtg                                                                               >  1:3282728/1‑150 (MQ=255)

                                                                        gTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCATTAAAGACGTTACAACGGACGATGAGTTACGTAGCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACggtgg                                                                              <  2:4096126/150‑1 (MQ=255)

                                                                           tgtgACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTaa                                                                           >  2:2542653/1‑150 (MQ=255)

                                                                            gtgACCCGTAATATCGACTGTTATGCTTTTTCACTGCCTAAATTTAAAGCCATTAAAGACGTTACAACGGACGTTGTTTTACGTATCTGGAAATATGGTTTAAAAAAAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTaaa                                                                          <  1:2526675/150‑1 (MQ=255)

                                                                             tgACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAg                                                                         >  2:3546675/1‑150 (MQ=255)

                                                                              gACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGc                                                                        >  2:1669145/1‑150 (MQ=255)

                                                                                cccGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCg                                                                      >  1:4700839/1‑150 (MQ=255)

                                                                                  cGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGAt                                                                    >  1:2303506/1‑150 (MQ=255)

                                                                                    tAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGAGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAATCCGATTa                                                                  >  1:3474740/1‑150 (MQ=255)

                                                                                      atatCGTGTGGTATGCGTTTTCCCTGCCTAAAATGAACGCCATAAAATATGTAACAAGGGACTTTGCGTTACTTATCTGTAATTATGTTTTAAAAAAAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAAt                                                                <  1:2248802/150‑1 (MQ=255)

                                                                                       tatCGACTGGTATGCGTTTACACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACTATGAGTTACGTATCTGTAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATc                                                               <  1:4701548/150‑1 (MQ=255)

                                                                                        atCGACTGGTATGCGTTTTCACTGCCGAAAATTAAAGCCAGTAAATAAGTTACAACGTACGATGAGTTACGTATCTGGAATTAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCt                                                              <  2:4617730/150‑1 (MQ=255)

                                                                                         tCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTTACGGTGGGTAAGCCGATTAAtaat                                                             >  1:175540/1‑147 (MQ=255)

                                                                                          cGACGGGTAGGCGTTTTCACTGCCGAAAATGAAAGCCCGTAAAGATGTTACAACGGACGATGAGTTACGTATCGGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTc                                                            <  2:3977012/150‑1 (MQ=255)

                                                                                           gACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTcc                                                           >  2:3860962/1‑150 (MQ=255)

                                                                                             cgggTATGCGTTTTCCCTGCCGAAAATGCACGCCAGTAACGACGTTCCAACGGACGATGCGTTACGTATCTGGAAATAAGGTTGACAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAg                                                         <  2:4585994/148‑1 (MQ=255)

                                                                                             cTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAg                                                         >  2:2241557/1‑150 (MQ=255)

                                                                                               ggTTTGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGTAAATATGTTTGAAAAATAAAAACGGCGCTAAACAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGAt                                                       <  1:1686431/150‑1 (MQ=255)

                                                                                               ggTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAATCCGATTAATCTTCCAGAt                                                       >  1:1003140/1‑150 (MQ=255)

                                                                                                  aTGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCAc                                                    >  2:2944509/1‑150 (MQ=255)

                                                                                                   tGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAACGCGCCGTTTTTTTTGACGGGGGGAAAGCCGCTTAATCTTCCAGATCAcc                                                   >  1:870059/1‑150 (MQ=255)

                                                                                                    gctttttCACTGCCTAAAATTAAAGCCAGTAAACAAGTTAAACCGGACGATTAGTTACTTATCTGTAAATAAGGTTTAAAAATAAAAACGGCGCGAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCg                                                  <  1:750761/147‑1 (MQ=255)

                                                                                                      gTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCa                                                >  2:3626179/1‑150 (MQ=255)

                                                                                                      gTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATATTCCAGATCACCGCa                                                >  1:2238322/1‑150 (MQ=255)

                                                                                                       ttttCACTGCCTAAAATTAAAGCCACTAAAGAAGTTACAACGGACTATGAGTTACTTATCTGTAAATAAGGTTTAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAg                                               <  2:2023031/150‑1 (MQ=255)

                                                                                                        tttCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGATTTTTTTTACGGTGGTAAATCCGATTAATCTTCCAGATCAACGCAGa                                              >  2:4065025/1‑150 (MQ=255)

                                                                                                          tCACTGCCGAAATTGAAAGCCCGTACAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAACACGGCGCTAACAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAg                                            <  2:854274/150‑1 (MQ=255)

                                                                                                          tCACTGCCGAAAATTAAAGCCTGTAAAGATTTAACAACGGACGATGTGTTACGTATCTGTAAATATGTTTTAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAg                                            <  1:3427525/150‑1 (MQ=255)

                                                                                                          tCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTACAGATCACCGCAGAAg                                            >  1:557870/1‑150 (MQ=255)

                                                                                                          ccACGGCCCAAAATCAACGCCATTAAAGACGTAACAACGTCCGTTGTTTTCCGTTTGTGCAAATATGGTTCAAAAAAAAACACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAg                                            <  1:4739109/149‑1 (MQ=255)

                                                                                                           cACTGCCGAAAATGAAAGCCAGTAAAGAATTTACAACGGACGATGAGTTACGTATCTGGAAATACGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGc                                           <  2:2711409/150‑1 (MQ=255)

                                                                                                              tGCCTAAAATTAACGCCATTAAAGAATTAACACGGGACGCTTCTTTACTTACCTGTAAATATGGTTAAAAAAAAAACACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGAt                                        <  1:4773922/150‑1 (MQ=255)

                                                                                                              tGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGAt                                        >  1:2811799/1‑150 (MQ=255)

                                                                                                                 cGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCCCAGAAGCGATAAc                                     >  1:1521657/1‑150 (MQ=255)

                                                                                                                     aaTGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTc                                 <  2:4120944/150‑1 (MQ=255)

                                                                                                                      aTGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCa                                >  2:891521/1‑150 (MQ=255)

                                                                                                                        taaaGCCATTAAATAATTTACAACGGACGATGAGTTACGTATCTGTAAAAAAGGTTCAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCAcc                              <  1:1627769/149‑1 (MQ=255)

                                                                                                                        taaaCCCATTAAATAATTAAAAACGGACGATGAGTTACTTATCTGTAAATAAGGTTTAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCAcc                              <  1:1906929/149‑1 (MQ=255)

                                                                                                                        gAAAGCCATTAAAGAAGTTACAACGTACGATGAGTTACTTATCTGGAAATAAGTTTGAAAAATAAACACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCAcc                              <  2:1877652/150‑1 (MQ=255)

                                                                                                                            gCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGa                          <  2:2359870/150‑1 (MQ=255)

                                                                                                                                  aaaGATGTAACAACGGACTTTTTTTTAGGTATTTGTAAATATGTTTTAAAAATAAACACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAAtggtg                    <  1:2363895/150‑1 (MQ=255)

                                                                                                                                   aagaagTTACAACGGACTATGTGTTACTTATCTGCAAATAAGTTTCAAAAAAAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAAtggtgg                   <  1:4062234/150‑1 (MQ=255)

                                                                                                                                   aagaagTTACAACGGACGTTGCGTTCCTTATCTGGAAATACGGTTTAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAAtggtgg                   <  1:2427189/150‑1 (MQ=255)

                                                                                                                                    agaagTTACAACGGACTATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGc                  <  2:1425732/150‑1 (MQ=255)

                                                                                                                                    agaagTTACAACGGACGATGAGTTACGTATCTGGAAATAAGTTTCAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGc                  <  1:4604823/150‑1 (MQ=255)

                                                                                                                                     gaagTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTTACGGTGGTAAAGCCGATTAATATTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCg                 >  1:3956711/1‑150 (MQ=255)

                                                                                                                                     gaagTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCg                 >  2:4007374/1‑150 (MQ=255)

                                                                                                                                      aagTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCGTCACCGTGACTGGGGGCga                >  2:683471/1‑150 (MQ=255)

                                                                                                                                      aagTTACAACGGACGATGAGTTACGTATCAGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTATTTTGTCGGTGGTAAAGCCGAATAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCga                >  2:2289550/1‑150 (MQ=255)

                                                                                                                                       agTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTGGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCgat               <  1:5098823/150‑1 (MQ=255)

                                                                                                                                         ttACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCgatga             >  2:3588761/1‑150 (MQ=255)

                                                                                                                                          tACAACGGACTATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCgatgat            >  2:3065495/1‑150 (MQ=255)

                                                                                                                                          tACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTTAATGGTGGCgatgat            >  2:533073/1‑150 (MQ=255)

                                                                                                                                            cAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTAACCGTGAATGGTGGCGATGAttt          >  1:3526977/1‑150 (MQ=255)

ACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTA  >  CP009273/4629176‑4629473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`